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Bromus tectorum is a highly invasive annual grass. The fungal pathogen Pyrenophora semeniperda can kill a large fraction of B. tectorum seeds. Outcomes in this pathosystem are often determined by the speed of seed germination. In this paper we extend previous efforts to describe the pathosystem by characterising secondary dormancy acquisition of B. tectorum. In the laboratory approximately 80% of seeds incubated at -1.0 MPa became dormant. In the field, seeds were placed in the seed bank in late autumn, retrieved monthly and dormancy status determined. The field study confirmed the laboratory results; ungerminated seeds became increasingly dormant. Our data suggest that secondary dormancy is much more likely to occur at xeric sites.

A three-year-old male western black-tailed rattlesnake (Crotalus molossus molossus), which was refusing food and losing weight was presented with a swelling on the right side of the head below the eye. An abscess was suspected and treatment with subcutaneous enrofloxacin was started immediately. After identification of the causative agents as Pseudomonas aeruginosa and Stenotrophomonas maltophilia, and following susceptibility testing, enrofloxacin treatment was replaced with marbofloxacin, intralesional gentamicin/betamethasone treatment and gentamicin administered subcutaneously at an increased terrarium temperature of 35 °C. Seven days later, the formed pus plug was debrided and a combined marbofloxacin/gentamicin/betamethasone treatment was continued for an additional seven days. The swelling disappeared. Marbofloxacin was continued for ten more days, after which time microbiology tests were negative for S. maltophilia and P. aeruginosa. The animal began to eat and gain body weight. To our knowledge, this is the first report of an abscess treatment in a rattlesnake and the first to demonstrate the effective treatment of a S. maltophilia-induced infection with a combination of marbofloxacin and gentamicin.

: Nutrient digestibility and parameters of nutritive value for ruminants of two winter wheat (Triticum aestivum L.) cultivars were evaluated by means of an in vivo balance trial performed by the regression method on two groups of heifers with an increasing proportion of grain in DM (from 6 to 46%). Sulamit and Rapsodia, chosen on the basis of the in vitro test from a set of 25 cultivars (grown in 2002-2004) reached significant differences in DM fermentability in vitro (by 43.7-78.6 ml/g DM, P < 0.05). In vivo digestibility of crude protein, nitrogen-free extract, organic matter, parameters of N retention, energy concentrations (metabolisable energy, net energy for lactation and for fattening) and parameters in the PDI system (especially PDIE) increased along with the grain proportion in the diet. At the comparable proportion of grain in the ration the positive differences were significantly higher (P < 0.05) for Sulamit than Rapsodia.

The effects of methanolic extract of Dracaena arborea on mean testicular weight, mean cauda epididymal sperm reserve, and testicular morphology were evaluated. A total of sixty mature male Sprague Dawley rats were divided into three equal groups. The first group (A) received distilled water while the other two groups (B and C) received orally the methanolic leaf extracts of Dracaena arborea in two doses (100 and 500 mg/kg, respectively) daily for 84 days. Following oral administration of the extract, mean testicular weight, mean cauda epididymal sperm reserve, and testicular morphology were determined on days 28, 42, 56, 70, and 84. The extract produced a significant and dose-dependent increase (P < 0.05) in the sperm number. There was also a significant increase (P < 0.05) in the mean testicular weights on days 70 and 84 of the extract administration. The testicular morphology remained unchanged while further testicular histology examination revealed increased spermatogenesis. It was concluded that the methanolic leaf extract of D. arborea has fertility enhancing properties.

The aim was to compare the efficacy, selectivity to sunflower and dissipation of three chloracetamide herbicides (acetochlor, metolachlor and pethoxamid) in dependence on precipitation after application. A small plot field trial was carried out with sunflower in Central Bohemia in 2010 and 2011. The remaining concentration of active ingredient in the soil of all studied herbicides was lower in the year with a higher temperature and a low level of total natural precipitation at the beginning of the growing season. Higher leaching of metolachlor and pethoxamid was recorded in irrigated plots. No leaching was found in the case of acetochlor. The highest leaching was found for metolachlor (9.2-25.5% in soil layer 5-10 cm). The highest phytotoxicity was found for acetochlor (9.8%) > pethox-amid (4.6%) > metolachlor (1.8%). The mean phytotoxicity in the irrigated plots was 6.9%, compared with 3.9% in the non-irrigated plots. The efficacy of the tested herbicide was affected by wet conditions. The highest efficacy on Chenopodium album, Amaranthus retroflexus, Echinochloa crus-galli and Solanum physalifolium was recorded after application of acetochlor and irrigation.

The paper presents the contents of phosphorus and potassium available in soil, soil sorption properties against the activity of alkaline and acid phosphatase. The fertilisation applied involved P, K, Mg, Ca and S: (P K Mg Ca S), (K Mg Ca S), (P Mg Ca S), (P K Ca S), (P K Mg S), (P K Mg Ca) as well as nitrogen fertilisation at the following rates: 0, 50, 100, 150, 200, 250 kg N/ha. The application of high nitrogen rates with no liming applied resulted in an unfavourable increase in hydrolytic acidity and a decrease in the total bases, cation exchange capacity as well as the sorption complex saturation with bases. According to the criteria provided in PN-R-04023, the soil investigated can be classified as the 3^rd class with an average available phosphorus (P_E-R). The analysis of Luvisol salinity demonstrated that the unbalanced mineral fertilisation applied did not trigger any chemical degradation. Significant values of the coefficients of correlation were recorded between the activity of alkaline and acid phosphatase and the parameters investigated (hydrolytic acidity, total exchangeable, base stauration, P_E-R). The calculated enzymatic index of soil pH (AlP/AcP) ranged from 0.11 to 0.72.

The dynamics and forming of European agriculture are determined by many considerably heterogenous and complicated processes and trends which influence mutually and moreover they work in a different way in developed and developing countries. An attention will be paid to basic global trends, the role of the Common Agricultural Policy, the influence of agrarian markets, the promotion of multifunctional agriculture etc.

Soil and water salinization are affecting an increasing number of countries in the world, especially in arid and semi-arid regions, and cause sensible reductions of agricultural land extension and of crop yields. Consociation with halophytic plants is a promising but not yet widely investigated strategy of salt stress reduction in crops. In this experiment, tomato plants were cultivated in saline conditions, alone and in consociation with three different halophytic species (Portulaca oleracea L.; Salsola soda L.; Atriplex hortensis L.). The salinity was brought either by the soil or by the irrigation water. Consociation with P. oleracea gave the best results in terms of increase of tomato growth and yields, while S. soda caused excessive nutritional competition against tomato due to its fast growth, undoing the positive effects of saline ions uptake. A. hortensis gave intermediate results. Salinity of water resulted in causing more severe stress on the plants, and consequently highlighted more the benefical effect of salt uptake performed by the halophytes on the main crop; salinity of soil on the contrary appeared to be less decisive, probably due to the leaching effect of the irrigation water.

Biochar (BC), the carbon-rich by-product resulting from pyrolysis of biomass, is used for bioenergy and increasingly as a soil additive for carbon sequestration and soil improvement. However, information about the effects of BC on forest productivity and reforestation success, especially on boreal and temperate forest soils, is scant. We examined the effects of two BC types (Canadian and Finnish) added in proportions up to 60 vol.% into a common alluvial silty soil on the growth of transplanted Norway spruce (Picea abies [L.] Karst.) seedlings in a growth chamber. We found no marked differences in seedling growth among the binary growing media mixes used. Seedling growth attributes (seedling height, terminal shoot growth, root volume) differed consistently only between the BC types in the highest proportion used. The terminal shoot growth differed overall among the two BC types. These results suggest that BC may be applied into mineral soils without detrimental chemical effects on tree plantation success. Our results provide foundation for further field research on the longer-term impacts of adding BCs to boreal forest soils.

The main role of sperm is the delivery of the paternal genome into the oocyte during fertilisation. However, several lines of evidence have indicated that mammalian spermatozoa contribute more than just their DNA, namely, they also deliver a large range of RNA molecules. Microarray analysis has revealed a complex population of 3000 different kinds of messenger RNA that are delivered to oocytes by sperm and ejaculated spermatozoa are estimated to contain about 0.015 pg of total RNA. Some of the transcripts encode proteins crucial for early embryo development. Messenger RNAs from sperm also help to protect the paternal genes, which have an integral role soon after fertilisation. The molecular participation of the oocyte during fertilisation is well understood but the function of the sperm in this process remains unclear. During spermatogenesis the structure of the male haploid genome is permanently modified. Transition proteins (TNPs), protamines (PRMs) and histones (HILS-spermatid specific linker histone) play a unique role in spermatid chromatin compaction. In this review, the structure and role of sperm RNA as well as chromatin organisation during spermatogenesis are discussed.

Ninety eight isolates of Rhizoctonia spp. were obtained from roots of pepper (Capsicum annuum L.) grown in Erzincan, Turkey during the period 2007-2008. The most prevalent multinucleate anastomosis groups (AG) were AG-4 (85.2%), followed by AG-2 type 1 (7.4%), AG-6 (5.0%), and AG-3 (2.5%). The population of binucleate Rhizoctonia spp. comprised AG-A (82.4%), AG-K (11.8%), and AG-G (5.9%). Rhizoctonia solani AG-3 and AG-6, as well as binucleate Rhizoctonia spp. AG-G and AG-K on pepper (C. annuum) were firstly determined in this study. During both in vitro and in vivo pathogenicity experiments differences in virulence level between R. solani and binucleate Rhizoctonia spp. isolates were observed. Isolates of R. solani AG-2 type 1 and AG-4 were the most virulent, binucleate Rhizoctonia spp. isolates of AG-A were less virulent, whereas binucleate Rhizoctonia spp. isolates of AG-G and AG-K were non-pathogenic.

We received a malformed Messinese Black goat foetus for examination. The foetus was part of a triple gestation, from which two male kids presented no morphological abnormalities and underwent regular slaughtering. X-ray examination and necropsy were performed. On the basis of gross and radiological findings the malformation was classified as acardius acephalus. In human medicine, acardius is a rare and severe abnormality reported as a unique complication of monozygotic twin pregnancies, and is known as twin-reversed arterial perfusion (TRAP).

The research results have shown that the enzyme pH index (0.49-0.83) confirmed the neutral or alkaline nature of the soils. Neither the changes in the content of available phosphorus nor in the activity of dehydrogenases, catalase, alkaline and acid phosphatase in soil were due to the factors triggering soil salinity; they were a result of the naturally high content of carbon of organic compounds, which was statistically verified with the analysis of correlation between the parameters. There were recorded highly significant values of the coefficients of correlation between the content of available phosphorus in soil and the activity of alkaline (r = 0.96; P < 0.05) and acid phosphatase (r = 0.91; P < 0.05) as well as dehydrogenase (r = 0.90; P < 0.05). To sum up, one can state that Mollic Gleysols in Inowrocław are the soils undergoing seasonal salinity; however, a high content of ions responsible for salinity is balanced with a high content of organic carbon, humus, phosphorus and calcium directly affecting the fertility of the soils analyzed. The activity of the enzymes depended on the natural content of carbon of organic compounds and not on the factors affecting the soil salinity, which points to the potential of such tests for soil environment monitoring.

A four-year field experiment was conducted in north-eastern Poland. The aim of the study was to determine the direct and residual effects of increasing doses of meat and bone meal (MBM) on the available phosphorus content of soil and the total phosphorus content in crops above ground biomass or grain. Experimental factor I was MBM dose (1.0, 1.5, 2.0 and 2.5 t/ha/year, and 2.0, 3.0, 4.0 and 5.0 t/ha/every second year), and experimental factor II was the year of the study (four consecutive years). The application of increasing MBM doses to slightly acidic soil insignificantly decreased its pH, but it did not change soil classification. The use of MBM as a fertilizer increased the levels of available phosphorus, compared to the treatments with mineral fertilization. The grain of winter triticale and winter wheat and the green matter of maize contained higher concentrations of phosphorus after the MBM application, in comparison to the plants receiving mineral fertilization. Phosphorus uptake by winter wheat and maize plants (dry matter basis) was higher in treatments with MBM (in particular applied every second year) than in treatments with NPK fertilization. Irrespective of the frequency of MBM application, phosphorus uptake by winter rapeseed was considerably lower, compared to the control plants.

The paper addresses the LEADER approach in the Czech Republic. Using documentary research and content analysis of the appropriate documents and the Local Action Groups information sheets, the paper firstly outlines the evolution of the LEADER approach in the Czech Republic (the paper points out the difference in understanding LEADER in the EU /focusing on capacity building and the use of intangible forms of capital/ and in the Czech Republic EU /focusing on investments/). The paper also analyses the participation of farmers and the farming related actors in the LEADER approach (approx. 30% of local action groups are composed by those actors, however, they mostly do not aim /similarly like non-farming actors/ at developing partnership but want to achieve the investments into production; that is why the Czech local action groups are rather quasi-partnerships; it is also reflected in a very low number of strategies aiming at the "adding value to local products" which is the closest to farmers /but it is the less opted theme: only 6% of projects/). The paper ends with the analysis of projects implemented under the LEADER scheme where the farmers participate. It shows that more than the integrated strategies, the Czech local action groups prefer the strategies of the multi-sector type. The paper also points out that the publicly available information about the activities of the local action groups is not sufficient, although the groups are funded from the public budgets. This fact makes the analysis more difficult as for the scientific merit but also contradicts the principles of democratic governance.

The paper presents the evaluation of data on precipitation totals and on SO₄^{2 -}concentrations and S-SO₄^{2 -} fluxes in lysimetric waters in a submontane beech stand. The records cover continually a 19-year period. The site is situated in the Kremnické vrchy Mountains, belonging to the West Carpathians Mountains. The material was sampled on two plots. The first had been clear cut immediately before the research started (1989), the second was covered with the original forest stand. In total, the highest SO₄^{2 -} concentrations on the open plot were measured four years after the intervention (1993) at the soil depth of 0.25 m (40.50 mg.l^-1). The lowest values were recorded in 1989 on the control plot at the soil depth of 0.25 m (4.54 mg.l^-1). The sulphate sulphur deposition was the highest in 1995 in the surface humus layer on the open plot (51.8 kg.ha^-1.year^-1). A very significant influence of precipitation totals on sulphur amounts deposited in the particular soil horizons was also confirmed. No interannual differences in SO₄^{2 -} concentration and S-SO₄^{2 -} fluxwere proved.

Fraser fir (Abies fraseri) Christmas tree plantations in North Carolina are infested by root rot caused by Phytophthora cinnamomi. This disease kills almost 100% of Fraser fir material and leaves the soil permanently infested. Control crossings of Mediterranean fir hybrids Abies cilicica × Abies cephalonica with Abies fraseri were performed to ensure possibly resistant hybrid material of desired Christmas tree parameters. Pollen of various clones of A. fraseri was shipped to the Czech Republic by NCSU. Control pollinations were performed in April/May 2010 and 2011 in our hybridization seed orchards. Female strobili were isolated in the period of their highest receptibility. Cones were collected during September. Cones were dried and completely disintegrated. Basic parameters of cones and seeds were assessed for each seed lot. Seed samples of the individual seed lots were X-rayed for assessment of the final share of full seeds. In 2010, the most successful combination CZ2 × NC81 brought 7% of viable seeds. In 2011 the most successful combination was CZ2 × NC26 with 18% of viable seeds.

In Europe, glyphosate resistant populations have developed in some weed species in perennial crops, including three species of the genus Conyza documented by the International Survey of Herbicide Resistant Weeds. Conyza spp. biology is reviewed in this paper and related to population dynamics and the development of resistant populations. Suboptimal growth stage at application, improper agricultural practices such as overreliance on glyphosate and long-term use of sublethal doses are identified as the most important factors of resistance development. Current control methods in perennial crops including mixtures of glyphosate with other active ingredients are discussed and effective weed management strategies are described to manage the development and spread of glyphosate resistant Conyza spp. in Europe.

Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. However, if these tools are to be used correctly and accurately, the techniques used must be standardised. In the present study, two molecular techniques for diagnosing Cryptosporidium infection in cows were compared to determine the optimal methods. For each technique, we tested two DNA extraction methods, several annealing temperatures for nested PCR reactions targeting the 18S, SSU rRNA (small subunit ribosomal RNA), and the GP60 (60 kDa glycoprotein) genes, and two types of DNA staining reagents, ethidium bromide and GelRed^TM. We determined that one of the tested protocols yields a higher purity of extracted DNA. Additionally, optimised temperatures for the nested PCR of the 18S and GP60 genes were established. Finally, we determined that the GelRed^TM dye was more sensitive than ethidium bromide, and its low toxicity facilitates handling and disposal and reduces environmental contamination.

The effects of land use practices on soil fauna, especially earthworms, are poorly known in coastal saline agricultural soils. Here we compare earthworm communities in six types of land use practice in the coastal region of China's Bohai Bay, namely uncultivated saline soil, two orchard (pear and winter jujube) lands, man-made forests (chinese ash), vegetable land and cropped land (maize). In addition, we recorded selected physicochemical properties of the soil. Soil organic matter content and total N were significantly higher under pear orchard and vegetable land than under the other land use practices, and their lowest values were observed from uncultivated saline soil. Vegetable land and pear orchard land showed a significantly higher abundance of earthworms than the other land use practices, whereas no earthworm was found in uncultivated saline soil. The sites under individual practices supported one to three earthworm species. Aporrectodea trapezoides species was present under four types of land use practice, and the biomass of this species accounted for more than 60% of the community. Vegetable land and pear orchard land supported richer earthworm community than the other land use practices, dominated by Aporrectodea trapezoides and Drawida japonica. These preliminary results indicated that land use practices have substantial effects on the abundance and composition of earthworm communities in saline soils.

Subsoil compaction can result in unfavourable soil physical conditions and hinder the root growth of maize. The effects of deep tillage and conventional tillage on soil physical properties and root growth of maize were studied during 2010-2011 at two sites (loam at Hebi and clay at Luohe) in central China. The results showed that soil penetration resistance, bulk density, water content and root length density were significantly affected by tillage, soil depth and year. Deep tillage had lower penetration resistance and lower soil bulk density, but higher soil water content than conventional tillage across years and depths. Averaged over the whole soil profile, deep tillage not only significantly decreased penetration resistance and soil bulk density, but significantly increased soil water content and root length density on loam, while deep tillage only significantly increased the root length density on clay. We conclude that deep tillage on the loam is more suitable for the root growth of summer maize.

Soil samples were collected at both jointing and maturing stages of maize and wheat to compare the effects of 4-year no-tillage (NT) and conventional tillage (CT) on seasonal variations of microbial biomass carbon (C), metabolic quotient, and invertase activity in a sandy loam soil in North China. Soil invertase activity significantly increased (P < 0.05) from summer to spring of the next year and then significantly decreased (P < 0.05) from spring to summer. With a delay of about 3 months, soil microbial biomass C and basal respiration altered in a similar pattern, while microbial metabolic quotient changed on the contrary. Compared with CT, the NT practice significantly increased (P < 0.05) soil organic C content, and tended to result in higher soil microbial biomass C and invertase activity, as well as lower soil microbial metabolic quotient, especially at the jointing stage of maize. Our results indicated that NT might play an important role in the improvement of soil microbial efficiency, especially at the maize seedling season.

The delimitation of less-favoured areas (LFA) on the territorial and the farm level in the EU member states varies in many ways. The Commission emphasizes the unification of criteria on the territorial level. But the EU countries use very different criteria for the eligibility area definition and for the eligibility on farm level. This makes the comparison very difficult. The CR belongs to the countries with a lower level of differentiation of payments rates. The eligibility area is restricted to the grassland in the CR and degresivity of payments is not applied. In particular, collective farms as the owners' association play an important social role in the country of the Czech Republic and some other countries in the Central and Eastern Europe. It would be necessary to find such differentiation of payment system that would reflect these specific conditions. The holdings with different size and different form of farming as well as the effect of other subsidies to holdings should be taken into account. The support potential within the axis III of the Rural Development Program would be necessary strengthen to elimination of the adverse impact due to further lay-off of the employees in agriculture

The paper characterizes the natural conditions and current state of floodplain forests in the area of Litovelské Pomoraví and proposes the protective management of the area in accordance with the European Natura 2000 system. The paper describes the geographical location and the natural conditions of the area of interest (climate, geology, geomorphology, hydrology and flood regime, soils, and vegetation). Six groups of geobiocene types were identified, classified and described in detail. Assessment of the ecological stability of the forest stand was carried out using biogeographical differentiation of the landscape and the outline of the forest ecosystem management is drawn based on the differentiation of the protected landscape area into zones with various levels of protection.

The production technology of high-quality vodka used in Russia involves filtration through activated charcoal. To approach the quality of renowned Russian vodka, one prominent Czech spirit-producing company installed on its production premises a filtration device including a charcoal column, and launched test runs during which different filtration conditions were tested. Samples collected during the test runs were analysed by GC-FID and GC-MS with the aim to compare their composition; sensory analysis was an integral part of the evaluation. The results documented a positive effect of charcoal filtration on the quality of produced vodka, which was not reduced when higher flow rates were applied.

The felling and skidding damage to residual trees was investigated in a selectively cutting operation in the Caspian forest of Iran. The logging operation was performed by chainsaw and cable skidder. Prelogging, after felling and skidding operations residual tree injuries (species, DBH and damage) were inventoried by systematic plot sampling. Two types of tree damage were observed: destroyed and injured. In this study felling operations mainly injured trees whereas skidding was the main cause of destruction. The percentage of destroyed and injured residual trees by felling operations was 1.4% and 3.4%, whereas the percentage of destroyed and injured residual trees by skid­ding operations was 5.2% and 11.1%. About 87% of destroyed trees were found in the DBH class smaller than 22.5 cm. Maple and Alder were the most damaged trees among the other trees species. Damage to the lower bole and wood damaged intensity were the most common type of injury. To reduce the stand damage, skid trails should be planned before felling and felling directions should be predetermined. In the selection cutting management, limiting logging damage to residual trees must therefore remain a major objective.

We evaluated changes in the content of total polyphenols and antioxidant activity of frozen spinach, peas, and sweet corn in relation to the storage period. Total polyphenol content and antioxidant activity in analysed samples were determined by colorimetric methods. Both parameters were analysed in frozen samples monthly and the changes were monitored during storage in a freezing box at a temperature of -18°C for 10 months. Freezing had a different influence on the levels of total polyphenol content and antioxidant activity in individual analysed samples. The greatest decrease in antioxidant activity during the entire period of freezing was recorded in spinach (79.4%), while the lowest decrease was observed in peas (26.8%). A relatively significant decrease in antioxidant activity was also found in sweet corn (62.7%). On the contrary, the greatest decrease of total polyphenol content throughout the period of freezing was found in peas (62.0%), and lowest decrease was recorded in sweet corn (only 5.0%). The total polyphenol content in spinach decreased by 43.1% after 10 months of storage.

In semi-arid regions of the United States, Pyrenophora semeniperda kills seeds of the winter annual Bromus tectorum. We report on pathosystem outcomes under manipulated water potential and temperature environments commonly observed within semi-arid environments for dormant and non-dormant seeds. We propose a range of outcomes for infected seeds. During summer, seeds remain dormant and are killed across a range of water potentials. During autumn, seeds survive by rapidly germinating or are killed if radicle emergence is delayed by intermittent hydration. In winter/spring, secondarily dormant seeds can be killed by the fungus. The only likely scenarios where seeds escape death include absence of infection (autumn, spring, or following autumn, germination) or infection in autumn when seeds germinate rapidly.

A two-year old male Syrian hamster (Mesocricetus auratus) was brought to the Surgery Clinic at the Istanbul University, Veterinary Faculty, Research and Practice Hospital, with a non-healing wound on the right upper eyelid. An ulcerated, bleeding wound was observed and diagnosed as an eyelid mass. The eyelid mass was excised and submitted for histopathological examination, which resulted in a diagnosis of meibomian adenoma. After excision of the mass, the right upper eyelid became deformed and adopted an abnormal shape. The surgical site was examined two weeks later. No abnormality was observed and the appearance of the right eyelid had returned to normal. Although there are many reports on benign tumours such as polyps, adenomas, haemangiomas and papillomas in hamsters, to the authors' knowledge, this is the first report on a meibomian adenoma in a Syrian hamster which was resolved by surgery.

The purpose of the studies was to determine the microorganisms communities in the soil under scorzonera cultivation with the cover crops using. The greatest total CFU of bacteria occurred in the soil from the experimental combination where oat ploughed over in spring or autumn was the cover crops. The smallest total CFU of bacteria was obtained after ploughing over the mulch of tansy phacelia. The use of oats and spring vetch had a positive effect on the population of bacteria from the genera of Bacillus and Pseudomonas. The smallest CFU of fungi was obtained from the soil where oat constituted the mulch. Alternaria alternata, Fusarium oxysporum, F. culmorum, Haematonectria haematococca and Thanatephorus cucumeris were most frequently isolated from the soil. The most isolates of the enumerated fungi were obtained from the soil cultivated in a conventional cultivation and after ploughing over the mulch of tansy phacelia plants. The most antagonistic bacteria and fungi occurred in the soil after ploughing over the mulch of oat, while the least in the conventionally cultivated soil.