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Organic farming is a topic often discussed in the scientific community and public space. The current European policy strongly supports the enhancement of organic farming concerning the environment, sustainability, and social aspects. It is generally assumed that a lower intensity of production, yields, livestock utility, fertilisation, and total costs should be compensated by premium prices, subsidies, or higher economic performance. Research revealed that achieving premium prices for organic products on the market is not obvious for organic farmers as some organic commodities have been sold on the conventional market. Our study focused on the price difference reached by the sample of farms on the organic and conventional markets for two organic commodities (winter wheat and milk) and the farm's economic results. We combined and compared data from two unique databases on organic farming in the Czech Republic. We used the statistical methods of ANOVA and regression for analysis. We found the difference between evaluated market prices. Price variability is wide in both types of organic and conventional markets but the influence of the prices on farm performance was not significant.

This study investigates the reciprocal relationship between non-farm employment and mechanisation adoption of smallholders in Vietnam using the longitudinal Vietnam Access to Resources Household Survey (VARHS) 2008–2016 dataset. By employing the correlated random effects with the Mundlak approach to address the selection bias from the unobserved heterogeneity of panel data and the instrumental variables regressions to treat the endogeneity issue of non-farm participation and mechanisation adoption, the findings revealed that non-farm employment and mechanisation adoption have a positive interactive relationship. The mechanisation adoption in agricultural production could save farm labour and allow farmers to engage in non-farm activities. Conversely, non-farm earnings could relax financial constraints and provide opportunities for farmers to invest in mechanisation. The agricultural labour shifting to non-farm work was replaced by hiring machinery services rather than machinery investment when the service market was available and cost-effective. Our study implies practical policies and actionable plans to encourage nonfarm employment and facilitate agricultural mechanisation toward sustainable agriculture and inclusive development in rural communities.

To select tree peony varieties with high ornamental value suitable for the hot and humid environment of the south Yangtze River area, 74 tree peony varieties from the Chenshan Botanical Garden in Shanghai were selected as experimental materials. The experiment involved initial selection for leaf humidity and heat tolerance, assessing the ornamental value during the flowering period of the following year and measuring a series of indicators such as the relative chlorophyll content of the leaves. Methods including principal component analysis, cluster analysis, and comprehensive membership function were employed. The results indicated that among the varieties assessed for humidity and heat tolerance, 43 obtained a composite score of 3.75 or above. Upon further assessment of the flowering period for these 43 varieties in the subsequent year, 14 varieties clustered in the top tier based on their scoring for ornamental value. Then, during the summer season, physiological indicators were measured for the previously selected 14 tree peony varieties. Combining these data with the comprehensive membership function, the humidity and heat tolerance of each variety were quantified. The synthesis of scores, combined with cluster analysis, revealed that varieties including ‘Yinhong Qiaodui’, ‘Shengge Jin’, ‘Linghua Zhanlu’, ‘Baixue Gongzhu’, ‘Di Guan’, and ‘Cunsong Ying’ exhibit both high ornamental value and tolerance to humidity and heat, making them suitable for cultivation in the south Yangtze River area.

Agricultural informatisation (AgI) is hailed as a 'game-changer' for farmers worldwide, even as climate change increases agriculture's vulnerability to climatic risks and threatens sustainable agrifood production. While AgI aspires to help alleviate hunger and poverty in smallholder farm households by improving on-farm productivity through the promotion of sustainable agricultural practices (SAPs), limited empirical evidence exists on the AgI–SAPs nexus, particularly under severe environmental stress such as drought. We analysed data from a survey of maize farmers in central Zambia – a country exemplifying the impact of severe drought, declared a national emergency and disaster – to explore whether and how AgI can optimise SAP adoption and improve crop yields. Given the potential endogeneity of AgI adoption, we employed a recursive bivariate probit (RBP) and endogenous-treatment regression (ETR) to estimate the former and the latter, respectively. We focused on adoption portfolios of three AgI tools – radio, television and mobile phones – and five SAPs: minimum tillage, residue retention, planting basins, improved seed varieties and irrigation. The results reveal that AgI adoption significantly influences SAP adoption, with varying impacts across different AgI and SAP portfolios. Importantly, the adoption of productivity-enhancing SAPs, particularly improved seed and drip irrigation, produced the largest yield effects (124.46 g/capita/day) for AgI adopters. This increase potentially contributes 43.21% towards daily maize-supply quantity, which is crucial for helping households meet the minimum recommended daily caloric intake. The study therefore underscores that AgI plays a critical role in improving yields through SAP adoption, serving as a compelling pathway for agricultural resilience, especially under adverse climatic conditions. These insights align with the United Nations Sustainable Development Goals (SDGs), particularly those aimed at zero hunger, climate action and poverty alleviation, which advocate re-thinking and transforming food-production strategies.

Hemp is becoming increasingly popular, and many new varieties are coming onto the market to meet the requirements of different industries. In this study, the seed and stem yield, seed nutritional properties and the biochemical characteristics of the inflorescences of seven European varieties (Fedora 17, Futura 75, KC Dóra, Monoica, Santhica 27, Tiborszallasi, USO 31) were investigated in a 3-year field trial. Futura 75 and Tiborszallasi stand out as varieties with the highest potential in the conditions of the experiment (humid continental climate with oceanic influences, heavy soil). Futura 75 achieved the highest seed yield (505 kg/ha dry matter), stem yield (8 036 kg/ha fresh matter), protein yield (140 kg/ha) and oil yield (181 kg/ha). There were no differences in protein content (average 21.0%) among varieties. The total unsaturated fatty acid content was as high as 87.6% at Tiborszallasi. The best ratio between omega-6 and omega-3 fatty acids was 3 : 1 in Tiborszallasi, which had also the highest oil content (30.2%), the highest total phenolic content (2.8 mg caffeic acid (CA)/g) and the best antioxidant potential (6.69 EC50 DPPH (2,2-diphenyl-1-picrylhydrazyl) mg/L). Most varieties had higher cannabidiol and tetrahydrocannabinol contents in the inflorescence at seed maturity (from 0.22 to 3.3 for cannabidiol (CBD) and from 0.00 to 0.32 for tetrahydrocannabinol (THC)) compared to full flowering (from 0.17 to 4.33 for CBD and from 0.00 to 0.52 for THC, on average 2.64% for CBD and 0.19% for THC), presenting an opportunity for dual-purpose use.

This study examines height growth and its driving factors in stools and standard trees, the two key structural components of coppice-with-standards stands. We focused on sessile oak [Quercus petraea agg. (Matt.) Liebl.] stands over 90 years old at two contrasting sites near Brno (South Moravian Region, Czech Republic): one actively managed and one left unmanaged for more than 40 years as a strict nature reserve. Tree heights were analysed using two-factor analysis of variance (ANOVA) with post hoc tests and multinomial logistic regression. A total of 1 239 trees were measured (584 stools and 655 standards). Standard trees were the tallest on average (21.8 m), significantly exceeding all the stool categories, whereas managed triple-stem stools were the shortest (15.97 m). Across all categories, unmanaged stands generally reached greater heights than managed stands did, except for standard trees. The forest type, exposure and slope had significant effects on height class probabilities, whereas elevation was important only for smaller trees. Overall, the results demonstrate that both the tree type and site conditions strongly influence height growth in Q. petraea coppice-with-standards stands, with management history leaving a clear biological legacy in the present-day stand structure.

Suaeda schimperi, a halophyte native to the Red Sea’s hyper-arid salt marshes, thrives in its extreme conditions (high salinity, minimal rainfall, and elevated temperatures). However, its adaptive tolerance mechanisms to these harsh conditions remain unclear. Herein, we investigated its growth responses and physiological mechanisms after short (5 days after treatment; DAT) and long-term (15 DAT) exposure to 0, 100, 200, and 400 mmol NaCl. Moderate salinity (200 mmol NaCl) enhanced growth, inducing 103.2% (5 DAT) and 40% (15 DAT) higher leaf biomass and 43.33% and 59.6% higher root biomass, respectively, compared to non-saline conditions. Deviation from moderate salinity reduced growth and disrupted ion balance, lowering K⁺, raising Na⁺, and increasing the Na⁺/K⁺ ratio, particularly under high salinity. The moderate salinity-enhanced growth was associated with increased chlorophyll, glycine betaine, glutathione, betacyanin, and betaxanthin, as well as higher antioxidant enzyme activity (polyphenol oxidase, peroxidase, catalase, ascorbate, and peroxidase) at 5 DAT. At 15 DAT, sugar accumulation and unsaturated fatty acids increased, while malondialdehyde and saturated fatty acids decreased. These findings reveal multiple adaptive strategies that support S. schimperi’s physiological stability under extreme environments and highlight its significance in ecological restoration and breeding salt-tolerant crops under escalating soil salinisation and climate change.

The response of carabid beetles to extensive livestock farming and vegetation structure in two traditionally managed 'dehesa' ecosystems was investigated. From March 2011 to January 2012, sampling was done, using pitfall trapping, on two forestry farms located in the Sierra de Hornachuelos Natural Park (Córdoba, Spain), both with hunting use, but one of them also with a heavy livestock load. On each forestry farm, two sampling plots were selected according to the vegetation structure. Faunal differences were proved through the ecological indices, and the faunal uniqueness was checked by the Coldwell and Coddington Complementarity Analysis. To identify the most influential factor on the carabid biodiversity, a Generalised Linear Mixed Model (GLMM) was performed. Results show that abundance and richness are higher in the plots with open vegetation, similarly like the number of recorded tribes. Nevertheless, the ecological indices do not reflect any significant differences. The complementarity between different vegetation structures exceeds that of the exploitation types. In fact, the GLMM analysis indicated that the livestock itself does not have a significant effect on the fauna. In addition, all exclusive, rare or endemic species came from the closed vegetation plots, suggesting that these areas may act as a reservoir of unique species in terms of biodiversity.

Soil salinisation is a key determinant in soil fertility decline, exerting a direct negative impact on soil organic carbon. In the context of global warming, investigating the response mechanisms of soil organic carbon pools with varying salinity levels to climate change is essential for accurately assessing the carbon cycle and emission potential of degraded soils. Based on soil samples (B1–B6) collected along a coastal salinity gradient, indoor incubation experiments were conducted at 15 °C and 25 °C to characterise soil respiration and its temperature sensitivity (Q10). Double-exponential models were used to simulate soil organic carbon (SOC) mineralisation, characterising active and stable organic carbon pools. The results demonstrated that the Q₁₀ value of the stable organic carbon pool (7–8% of SOC mineralisation) was 103% higher than that of the active organic carbon pool (the initial 1% of SOC mineralisation). The Q₁₀ value of the stable organic carbon pool was 32.6% higher at the high-salinity sites (B1, B2) than at the low-salinity sites (B4, B5). Soil organic carbon, total nitrogen (TN), and total salt (TS) were key regulators of Q₁₀. The Q₁₀ of the active organic carbon pool correlated positively with SOC and TN but negatively with TS, whereas the stable pool showed the opposite trends. The stable organic carbon pool exhibits a salinity-amplified Q₁₀, implying that predictive models must account for this mechanism to avoid substantially underestimating carbon losses from degraded saline soils.

This study aims to demonstrate that Andrographolide, an herbal immunostimulant, can influence M1 macrophages to inhibit inflammation, including the growth of endometriosis caused by inflammation. This study evaluates the effects of Andrographolide on suppressing endometriosis through M1 macrophage activity. This post-test-only experimental study involved 42 female Balb/C mice, which were divided into six groups: Group N, KN (endometriosis without therapy), and KP (endometriosis + dienogest), P1 (endometriosis with 0.05 mg/kg), P2 (endometriosis + 0.1 mg/kg Andrographolide), and P3 (endometriosis + 0.2 mg/kg Andrographolide). Peritoneal fluid was aspirated, and the peritoneum was cut and stretched to observe the extent of the endometriosis. This study compared the formation of ectopic endometrial lesions and analysed the M1/M2 ratio in each group that received standard therapy versus Andrographolide therapy. The results from peritoneal fluid flow cytometry indicated that M1 was more dominant than M2 compared with KP. The levels of M2 in the P2 and P3 groups were also significantly lower than in the KN group. This study demonstrated that Andrographolide may enhance the regulation of M1 macrophage differentiation, acting as a precursor to endometriosis growth by suppressing M2 phenotypes that promote the condition.

Biofortification provides a sustainable, pragmatic strategy to address the lack of vitamin A and the associated health complications. The objectives of the study encompassed the quantification of the carotenoid content of 147 maize inbred lines, the identification of variable regions within the ZEP1 gene, the correlation of these observed variances in the presence of this gene with carotenoid content, and the identification of lines harbouring the favourable alleles of the crtRB1 gene. The observed correlations among the carotenoids synthesised by distinct branches of the biosynthetic process were both significant and positive. Utilising gel-based genotyping, 24 lines with contrasting carotenoid profiles were selected, evaluated and sequenced. Analysis of the variation in the sequence classified these lines based on their similarities to give 8 allele groups. The findings highlight that inbred lines both group 1 and group 8 exhibited significant associations with the carotenoid content of the lines. Specifically, ZEP1_7852, a discernible variation belonging to group 8, was found to be significantly associated with zeaxanthin content and total carotenoid content. Furthermore, 25 lines were found to have provitamin A content above 15 μg/g, harbouring the favourable alleles of the crtRB1 gene using KASP SNP zm0016. These lines can serve as parents for source populations and hybrids, leading to the further enhancement of provitamin A in maize.

Osteosarcoma (OSA) is the most prevalent and aggressive malignancy of canine bones. Etoposide is an effective chemotherapeutic agent for cancer treatment, whereas ellagic acid is a naturally occurring compound with antioxidant and anticancer properties; however, both are inhibitors of the topoisomerase enzyme. In this study, the synergistic/additive effect of etoposide, known to have a growth-inhibitory impact in canine osteosarcoma (OSA) cells, and ellagic acid was investigated. The mechanism by which this effect inhibits cell growth at lower etoposide doses was also examined. The IC₅₀ values of both agents were determined, and possible combination doses were generated accordingly and applied to canine OSA cells. The apoptotic effects of the combinations were evaluated based on DNA breaks and the activity levels of caspase 3, 8, and 9. These findings were supported by the expression levels of Bcl-2, Bax, and Bid genes, as well as the AO/EtBr staining method. The effects on cell cycle and proliferation were analysed through survivin and NF-κβ gene expressions. Antimetastatic effects were determined using invasion and migration assays. EA is a potential therapeutic agent for cancer treatment. In combination with ET, a higher anticancer efficacy was demonstrated compared to etoposide alone. Potential treatment side effects can be reduced by enabling the use of lower drug doses.

β-Hydroxy-β-methylbutyrate (HMB) is a metabolite of the essential amino acid leucine, which can be produced naturally in mammals and is also found in trace amounts in citrus fruits and fish. Studies have shown that HMB plays an important role in maintaining human health by improving muscle health and inhibiting muscle catabolism. This review summarises the synthesis and metabolism of HMB and discusses its potential use as a nutrient, highlighting and analysing the importance of HMB supplementation for athletes' physical recovery and the treatment of muscular dystrophy-related diseases between 2019 and 2025. This study will help us to deepen our understanding of the application of HMB as a dietary supplement for the treatment of different diseases, providing the latest insights into its sustainability.

Curcuma longa L., known as turmeric, is a plant species belonging to the Zingiberaceae family. Turmeric is generally spread in Asian countries. It is used in many sectors, especially spices. It has been predicted that increasing climatic changes will affect the agricultural crop pattern. To provide an alternative crop for countries with sub-tropical climates, such as Turkey, the yield and quality values of turmeric grown under the greenhouse and shade net conditions were determined in the present study. Moreover, morphological traits such as plant height, tillering number, and leaf area were determined. The highest fresh yield (1 333.67 g/plant) was obtained under greenhouse conditions. Furthermore, the highest antioxidant value (3.01 IC50 mg/mg 2,2-diphenyl-1-picrylhydrazyl (DPPH)) was obtained under shade net conditions, while the highest total phenolic content (6.88 mg gallic acid equivalent (GAE)/g) was obtained under greenhouse conditions. Curcumin reached the highest level (1.79%) in greenhouse conditions. While the essential oil ratio varied between 5.22 and 7.32%, ar-turmerone, α-turmerone, and β-turmerone were determined as the main components in the essential oil. According to the results, turmeric can be grown in greenhouse conditions in subtropical regions.

This study examined the effects of chitosan films enriched with various essential oils on the quality characteristics of rainbow trout burgers stored at (4 ± 1 °C) for 21 days. Five groups were prepared: a control group (C), a chitosan film group (CF), and groups of chitosan films enriched with 1% oregano essential oil (OEO), citrus essential oil (CEO), and rosemary essential oil (REO). Microbiological counts and physicochemical factors were assessed. Significant differences (P < 0.05) in physicochemical properties were observed among the treatments, with OEO showing the lowest pH (6.67), the lowest peroxide value (PV; 12 meq O₂·kg^–1), and the lowest thiobarbituric acid reactive substances (TBARS) level [1.159 malondialdehyde (MDA)·kg^–1]. Microbial results indicated that the shelf life of the treated groups was extended by up to 9 days compared to the control. The lowest counts of Enterobacteriaceae, yeast and mould, and lactic acid bacteria in OEO were 5.17, 4.87, and 5.10 log CFU·g^–1, respectively, while the lowest counts of psychrophilic and mesophilic bacteria were observed in the REO group, at 6.34 and 6.29 log CFU·g^–1, respectively. In conclusion, combining essential oils, particularly oregano and rosemary, with chitosan effectively enhances seafood freshness and extends its shelf life.

Optimising the feed composition, especially the forage choice, and reducing costs are essential for improving dairy production efficiency. Different forage sources and proportions were used to formulate rations containing equal energy and protein, and their effects on rumen microbiota and milk production performance of dairy cows were evaluated in two experiments. In experiment 1, thirty lactating cows (235 ±13 d postpartum; milk production 29.1 ± 1.0 kg/day) were divided into Pangola and Bermuda groups. In experiment 2, twenty lactating cows (79.9 ± 8.1 d postpartum; milk production 34.7 ± 0.6 kg/day) were divided into Pangola and oat groups. In experiment 1, the Simpson index for rumen microbiota of the Pangola group was significantly higher than in the Bermuda group (P < 0.05). Analysis of the weighted unique fraction (UniFrac) distances indicated significant differences in the beta diversity of the community composition of rumen microbiota between Pangola, Bermuda and oat groups in both experiments (P < 0.001). The relative abundance of Prevotella brevis was significantly higher in the Pangola group than in the oat group in experiment 2 (P < 0.05). The somatic cell counts (SCCs), C18:0, and C18:1 in milk were significantly higher in the Bermuda group than in the Pangola group (P < 0.05) in experiment 1. On the other hand, milk crude protein (CP) and solids-not-fat (SNF) were significantly higher in the Oat group than in the Pangola group (P < 0.05) in experiment 2; however, milk urea nitrogen (MUN) was significantly higher in the Pangola group (P < 0.05). In conclusion, a switch of forage (Pangola vs Bermuda) at a lower proportion of the diet under the high forage level condition (experiment 1) caused only minor changes in rumen microbiota diversity (Simpson index, beta diversity) and milk production performance (milk SCCs, C18:0 and C18:1). On the other hand, a switch of forage (Pangola vs oat) at a higher proportion of the diet under the low forage level condition (experiment 2) resulted in greater changes in rumen microbiota diversity (beta diversity, relative abundances of bacterial taxa, P. brevis relative abundance) and milk production performance (milk CP, SNF, and MUN).

Allelopathic plants release phytotoxic compounds that contribute to their invasiveness by suppressing nearby species. However, it remains unclear which exact mode of action (MOA) underlies the allelopathy. This study explores the allelopathic mechanisms of Turnera subulata on the recipient indicator plant choy sum using a metabolomics approach. Briefly, T. subulata leaf aqueous extracts (LAEs) at different concentrations (0.0, 0.1, 1.0, 10.0, 50.0, and 100.0 mg/mL) were sprayed at 100 mL/m² on choy sum seedlings at the two to three leaf stage. After 21 days, the Soil Plant Analysis Development (SPAD) values and photosynthetic pigments of the exposed choy sum were measured, and their metabolites were subjected to a gas chromatography-mass spectrometer (GC-MS) analysis. The results revealed a 25% decrease in the SPAD, a reduction of 65% (chl a) and 71% (chl b), and a 45% reduction in the stomatal length at 100 mg/mL. A total of 15 significant metabolites (P < 0.05) with variables important for the projection score exceeding 1 (VIP > 1) were selected as the important biomarkers. These metabolites were identified as amino acids, carbohydrates, and fatty acids. The findings reveal the allelopathic potential of T. subulata and provide insights into the response of choy sum in response to the allelopathic activity of T. subulata LAEs.

Since 2020, the National Agricultural Research Institute (INIA) has conserved a Creole sheep flock at INIA Las Brujas. This study genetically characterised this population and compared it with six others: San Miguel National Park Creoles, commercial farms Creoles, Brazilian Creoles, Corriedale, Merilin and Soay sheep. The analysis included 628 individuals and 31 392 autosomal SNPs. Soay and San Miguel National Park Creoles exhibited the lowest genetic diversity (Ho = 0.266 and 0.279) and highest inbreeding (F_HOM: 0.283 and 0.249; F_ROH: 0.199 and 0.202). Merilin and Corriedale showed the highest genetic diversity (Ho = 0.351 and 0.364), while Brazilian Creoles had the highest Ho (0.327) among Creoles. Short runs of homozygosity (ROH) segments (≤4 Mb) predominated, with San Miguel National Park and INIA Las Brujas Creoles exhibiting the highest numbers of ROH (22 773 and 16 762, respectively). Fixation index (Fst) and Reynolds distances highlighted INIA Las Brujas Creoles and Soay as the most distinct (0.318 and 0.321, respectively). INIA Las Brujas Creoles also showed notable differentiation from San Miguel National Park Creoles (Fst = 0.269; Reynolds = 0.272). Principal component analysis (PCA) revealed clear clustering, with Corriedale and Merilin closely related (Fst = 0.060; Reynolds = 0.068). Admixture analysis indicated distinct ancestries for Soay, Corriedale and San Miguel National Park Creoles, while commercial and Brazilian Creoles showed significant admixture. INIA Las Brujas Creoles exhibited a distinct ancestry with traces of Corriedale. Phylogenetic analysis confirmed the divergence between Creole and Corriedale/Merilin populations. Results suggest the historical genetic exchange among INIA Las Brujas Creoles, Corriedale, and commercial farms Creoles, while high differentiation of San Miguel National Park Creoles reflects their closed status since 1929.

This study reports layered double hydroxides (LDHs) modified wheat straw biochar (W-B), denoted as (LDH/W-B), as an efficient adsorbent material for removal of lead (Pb²⁺) ions from aqueous solution. This study also juxtaposes the adsorptive performance of LDH/W-B with W-B for Pb²⁺ removal. W-B was prepared via pyrolysis of wheat straw in a muffle furnace, using a controlled heating rate of 5 °C per min to reach 600 °C over a duration of three hours. Subsequently, LDH/W-B was synthesised using the co-precipitation method. Both resulting adsorbents were characterised for surface morphology and functional groups by means of scanning electron microscope (SEM) and Fourier transform infrared (FTIR), respectively. The influence of key adsorption parameters on the adsorption efficiency of W-B and LDH/W-B was systematically evaluated. At 60 min, the maximum Pb²⁺ removal efficiency was observed to be 78.21% for W-B and 92.4% for LDH/W-B. An increase in adsorbent dosage from 0.05 to 0.7 g and at a contact time of 1 h further enhanced Pb²⁺ removal, achieving efficiencies of 97% for W-B and 99% for LDH/W-B. The optimal conditions for maximum Pb²⁺ removal were determined to be 0.3 g of adsorbent (W-B and LDH/W-B), an initial heavy metal concentration of 10 mg/L, and a contact time of 1 h. Pb²⁺ removal data of W-B and LDH/W-B best fitted to the Langmuir isotherm and pseudo-second order kinetic model, which confirmed the dominance of chemisorption of Pb²⁺ ions. Additionally, the maximum theoretical adsorption capacity for Pb²⁺ is close to the experimentally obtained values, suggesting that the adsorption of Pb²⁺ primarily occurs through monolayer formation on the surface of both adsorbents. Overall, this study demonstrates that LDH/W-B is a highly promising adsorbent for Pb²⁺ removal in wastewater treatment applications.

A quarantine organism, the bacterium Xylella fastidiosa (Xf), is a xylem-inhabiting, vector-transmitted, Gram-negative, and very slow-growing bacterium in the Lysobacteraceae (earlier Xanthomonadaceae) family. The spreading of X. fastidiosa over long distances occurs mainly via import/export human-mediated transportation of mainly latently or symptomatically infected plant material. Short-distance distribution is usually by xylem sap-feeding insects. Until now, the presence of X. fastidiosa has not been reported or studied in Poland. During our study, over 500 individuals from the four families: Cicadellidae, Aphrophoridae, Delphacidae and Membracidae were collected in different geographical regions of Poland. Real-time PCR with primers for rimM gene and nested PCR to detect X. fastidiosa, using DNA extracted directly from selected insects known as potential vectors of X. fastidiosa, did not confirm the bacterium's presence in these insects.

Decision support systems (DSSs) enable dairy farmers to make informed and timely decisions on herd health management. However, the lack of a disease scoring system by category and severity limits the application of this approach. In this study, we developed an innovative approach to dairy herd health management by establishing a novel scoring system for dairy herd health management aimed at providing a more nuanced understanding of disease impact. For this purpose, we retrieved 5-year data from 2 558 disease diary records of 798 primiparous and multiparous cows housed on a Czech farm and classified 125 production diseases into six categories, namely lameness, mastitis, postpartum diseases, digestive system, reproductive diseases and other diseases. Based on this metric, we developed a data-driven DSS for farm management. Using this DSS, we identified markers of disease categories for efficient veterinary monitoring on dairy farms. This DSS highlighted a decreasing trend of average monthly disease scores, yet the prevalence of postpartum and other diseases increased during the same period, due to changes in reproduction management within the herd. These findings underscore the need for data-driven targeted interventions for promoting the herd health. Therefore, our scoring model not only provides a comprehensive framework for dairy herd health monitoring and improvement but also advances dairy farming by providing a decision support system easily applicable to dairy farms based on available data recorded in disease diaries.

Lameness and mastitis are two of the most economically important issues for the dairy industry. This study aimed to obtain a clearer analysis of the link between mastitis and lameness in dairy cows using the Mastitis Score and detecting the pathogens that predominate in dairy cows’ milk samples with claw diseases. During routine claw trimming, milk samples were collected from dairy cows in two Slovak dairy farms. Out of 558 cows presented for claw trimming, 144 cows (25.8%) exhibited lameness from claw diseases. The most prevalent disease in our study was digital dermatitis (43.1%), followed by toe necrosis (41.7%), and Rusterholz ulcer (15.3%). Udder inflammation in clinical form was diagnosed based on clinical examination of individual udder quarters, and sensory evaluation of milk from each quarter and in the subclinical form by the CMT. Mastitis was detected in 80 cows with claw diseases, clinical mastitis in 14 cows (17.5%), and subclinical mastitis in 66 cows (82.5%). On both farms, Mastitis Scores were significantly higher in dairy cows affected by claw diseases than in the non-lame ones. In mastitic cows affected by claw diseases, environmental pathogens dominated the infected milk samples. Our findings showed that cows with claw disease were more likely to have mastitis.

The research was conducted in a long-term stationary experiment established on light grey forest surface-gleyed soil in 1965. Data presented in this study were collected during 2022–2024 growing seasons within the framework of this long-term experiment. The experiment is registered in the NAAS long-term field experiments registry (certificate No. 29) and the Global Long-Term Agricultural Experiments Network (GLTEN). The study examined the effect of growing red clover in a four-field crop rotation on nutrient balance at different fertiliser and lime doses and ratios. Red clover was used for feed and feed-green manure purposes. The research aimed to substantiate optimal methods of utilising this valuable forage crop and optimise fertilisation systems to ensure sustainable agricultural development. Growing the first cut of red clover for feed purposes and the second as green manure with fertilisation (N₁₀₅P₁₀₁K₁₀₁ + organic fertilisers + liming) ensures a positive surface balance of 402 kg/ha of nitrogen, 150 kg/ha of phosphorus, and 204 kg/ha of potassium. These data are almost twice higher than indicators under minimal fertilisation doses. Despite the reduction in symbiotic nitrogen fixation from 217 kg/ha to 147 kg/ha when growing red clover in crop rotation with intensive fertilisation, it remains an effective phytobiological ameliorant.

This study aimed to evaluate the therapeutic efficacy of a ceftiofur-neomycin combination against a pathogenic multidrug-resistant Escherichia coli strain, KECS-0513, isolated from pigs, using a combination of genomic, in vitro, and in vivo approaches. The minimum inhibitory concentration, minimum bactericidal concentration, and checkerboard assays were performed. Time–kill assays were used to assess bactericidal activity over time, and a murine intraperitoneal infection model was used to evaluate survival outcomes following treatment with monotherapies or combination regimens. Whole genome sequencing indicated that KECS-0513 harboured multiple resistance genes, including bla_TEM-1B, sul3, aadA12, aad(3)-IVa, aph(3’)-Ia, and aph(4)-Ia. The resistance genes were localised within a mobile, element-rich plasmid. In vitro checkerboard assays revealed an additive interaction between ceftiofur and neomycin (fractional inhibitory concentration index = 1.0), and time–kill experiments demonstrated enhanced and sustained bacterial clearance with combination therapy. In vivo infection studies in mice demonstrated that the dual treatment resulted in a substantially higher survival rate (66.7%) compared to treatment with either agent alone (33.3% for each). These results support the practical application of ceftiofur-neomycin combination therapy for controlling swine-associated multidrug-resistant E. coli while minimising the risk of resistance emergence.

Protective cultures are selected microorganisms that inhibit undesirable microbiota through metabolic activity, thereby improving the safety and shelf life of dairy products. This study evaluated the effect of protective cultures on the physicochemical, microbiological, and sensory properties of fresh cheese during 21 days of storage at 6–8 °C. Three variants were prepared: a control with the starter culture CHN-19 and two experimental samples containing FreshQ^® Cheese 3 and FreshQ^® 9 (Lacticaseibacillus rhamnosus). The application of protective cultures resulted in a slightly faster acidification but did not significantly affect dry matter or fat content. Microbiological analysis revealed higher counts of lactic acid bacteria and generally lower counts of psychrotrophic microorganisms in samples containing protective cultures on several storage days, while Escherichia coli and moulds were not detected. Sensory evaluation indicated that cheeses with protective cultures, particularly FreshQ^® 9, maintained a pleasant flavour and appearance even after 21 days of storage, whereas the control sample became sensorially unacceptable after approximately two weeks. The application of protective cultures therefore enhances the sensory stability and microbial safety of fresh cheeses without adversely affecting their technological characteristics.

Mediterranean Quercus forests have great ecological importance but face numerous threats, including pests. The spongy moth, Lymantria dispar L., is a major oak defoliator across its geographical range and has a natural enemy complex that may control its population dynamics. This study aimed to investigate candidate predators (Coleoptera: Carabidae) and parasitoids (Hymenoptera: Encyrtidae, Ichneumonidae, Pteromalidae, Braconidae, Bethylidae, Ceraphronidae, Eulophidae, Eupelmidae and Trichogrammatidae; and Diptera: Tachinidae), for the control of L. dispar in two areas in Andalusia (Spain). We studied 10 Quercus stands (Q. suber, Q. ilex, and Q. pyrenaica), with different L. dispar infestation level. Insects were collected using pitfall and cross-vane traps, during the defoliator's larval period. Four genera comprised 92.2% of all the Carabidae predators found: Steropus Dejean (34.1%), Carabus L. (28.4%), Calathus Bonelli (15.9%), and Platyderus Stephens (13.8%); and four Hymenoptera families comprised 93.7% of the parasitoid specimens collected: Encyrtidae (61%), Ichneumonidae (17.5%), Pteromalidae (10.7%), and Braconidae (4.5%). Both the natural enemy assemblage composition and the abundance per tree varied between geographical areas, as well as between levels of defoliator infestation. The candidate enemy complex was markedly diverse and abundant in stands not infested by L. dispar, where no insecticides had been applied. Our results suggest the importance of generalist predators as natural enemies of L. dispar.

Agricultural carbon emissions (ACE) is a critical contributor to global greenhouse gas emissions, which have already become a common challenge for global carbon reduction. As a major agricultural producer and largest carbon emitter, China has made great efforts to reduce ACE. Using the panel data of 30 provinces in China from 2011 to 2022, this study explores the heterogeneous impacts of formal environmental regulations (FER) and informal environmental regulations (IER) on ACE. The results reveal that both FER and IER have significant effect on reducing ACE, with FER showing a more pronounced effect. The mechanism analysis indicates that agricultural technological innovation and planting structure adjustment play important mediating roles in this impact mechanism. The effect of FER is more remarkable in major grain producing areas than in non-major grain producing areas, while the effect of IER is completely opposite. Compared with coastal regions, both FER and IER have significant inhibitory effect on ACE in inland regions. Additionally, the marketisation level may reinforce the inhibitory effect of both FER and IER on ACE. Based on the empirical results, this study suggests to strengthen the synergistic effect of FER and IER, promote agricultural technology innovation, and formulate targeted policies according to regional differences.

Developing and employing new, sustainable, and eco-friendly biostimulants that enhance plant growth and alleviate the harmful effects of environmental challenges is a major focus for many researchers. Salt stress is a critical constraint on plant growth and a limiting factor in crop productivity, particularly during the early developmental stages in the nurseries. Syzygium cumini (L.) Skeels (Java plum) is an important fruit tree and widely cultivated in gardens as an ornamental plant. This study was designed to develop Cassia javanica subsp. nodosa leaf extract (CLE) as a new sustainable and eco-friendly biostimulant capable of triggering the metabolic adaptation to salt stress in Java plum seedlings grown in nurseries. CLE successfully mitigated reductions in growth, biomass yield, and secondary metabolite production caused by salinity. Although salt stress depressed morphological characters and biomass yield, CLE foliar spray enhanced these parameters. Moreover, CLE enhanced the ferric reducing antioxidant potential, catalase, and superoxide dismutase enzyme activities, increased phenolic content, and reduced hydrogen peroxide (H₂O₂) accumulation and lipid peroxidation. Additionally, CLE application increased seedling biomass and stimulated antioxidant activity, osmoprotectant accumulation, and overall tolerance to salinity stress. These observations provide new insights into CLE’s potential as an eco-friendly biostimulant for enhancing salt tolerance in Java plum seedlings.

Changes in the content of mineral nutrients (Ca, Mg, K, Na) and risk elements (Mn, Cd) in the assimilatory organs of selected plant species were studied along the altitudinal gradient of A‒D zones polluted by alkaline emissions from the magnesite factory Lubeník (Slovak Republic). Multivariate statistical analysis and comparison with background values in other studies demonstrate persistent intoxication of some plants by Mg (all study plants), K (Lactuca saligna, Dryopteris filix-mas), Mn (Quercus polycarpa, Carpinus betulus, Betula pendula, Lactuca saligna) and Cd (Quercus polycarpa, Carpinus betulus, Betula pendula, Lactuca saligna). Overall, Lactuca saligna accumulated the highest amounts of Mg, Cd, Na and K near the magnesite plant, suggesting its potential as an effective bioindicator of elemental pollution. Unbalanced Ca/Mg ratios, lower than 1, were recorded predominantly in all plant species sampled near the magnesite plant; unbalanced K/(Mg + Ca) ratios were predominantly in woody species.

Premature ovarian failure (POF) is a significant cause of infertility and is often linked to autoimmune aetiologies. Lipopolysaccharide (LPS)-induced inflammation is a well-established model of autoimmune POF in rodents. Immunomodulatory treatments involving corticosteroids, frankincense, and targeted secondary antibodies have been hypothesised to mitigate the autoimmune response, reduce anti-ovarian antibody (AOA) levels, and restore ovarian function in an LPS-induced POF rat model. A POF model was established in female albino rats via the intraperitoneal injection of LPS. The rats were then divided into groups that received no treatment (LPS control), dexamethasone (DEX-treated LPS-treated rats), methylprednisolone (MP-treated LPS-treated rats), frankincense (Frankincense-treated LPS-treated rats), or secondary anti-ovarian antibodies (secondary Ab-treated LPS-treated rats) for 3 to 4 weeks. The serum levels of AOA, 17β-oestradiol, follicle-stimulating hormone (FSH), and luteinising hormone (LH) were assayed via commercial enzyme-linked immunosorbent assay (ELISA) kits. Ovarian tissues were examined histopathologically to assess structural damage and recovery. LPS induction successfully created a POF phenotype, as evidenced by significantly elevated AOA levels (P < 0.001), reduced 17β-oestradiol (P < 0.001), elevated FSH/LH (P < 0.001 and P < 0.05, respectively), and severe histopathological damage, including follicular atresia. All the treatments restored 17β-oestradiol levels. Secondary antibody therapy was most effective, normalising all hormonal parameters, significantly reducing AOA levels, and demonstrating complete histological recovery with healthy follicles and corpora lutea. MP potently suppressed AOA but paradoxically elevated FSH, without improving ovarian histology. DEX and frankincense showed intermediate efficacy, improving some hormonal and serological markers but failing to achieve full histological restoration. These findings demonstrate that targeted immunotherapy using secondary antibodies is superior to broad immunosuppression or anti-inflammatory treatment for restoring ovarian function in patients with autoimmune POF. While corticosteroids effectively reduce AOA titres, they may not reverse ovarian damage and can disrupt the hormonal balance. This underscores the need for precise, biomarker-guided therapies over nonspecific immunosuppression in patients with autoimmune ovarian insufficiency.