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The migration and transformation of soil phosphorus (P) are essential for agricultural productivity and environmental security but have not been thoroughly elucidated to date. A 10-year field study was conducted to explore the effects of conventional tillage (CT) and no-tillage with maize residue management (NT-0, NT-33%, NT-67% and NT-100%) on P contents and phosphatase activities in soil layers (0-5, 5-10, 10-20 and 20-40 cm). The results showed that soil available P content and phosphatase activities were higher in no-tillage with maize residue than CT. Soil moisture and pH were significantly positively correlated with soil available P. Higher organic P contents and lower inorganic P contents in the 0-5 cm soil layer were found in the treatment NT-67% compared with other treatments. According to the structure equation model, the source of available P was inorganic P in NT-33%, while organic P in NT-67%. This study demonstrated that the variation of dominant mechanisms involved in soil P migration and transformation were dependent on residue input amounts, and NT-67% might play an important role in the maintenance and transformation of soil organic P.

In field experiments over three vegetation periods (2010-2012) we studied impact of the CULTAN (controlled uptake long term ammonium nutrition) method on yield and yield parameters of kernel maize. The field experiments were conducted at three sites with different soil-climatic conditions. CULTAN treatments were fertilized once with the total amount of nitrogen using an injection machine (at the canopy height of 20 cm) and compared to conventional fertilization with calcium ammonium nitrate application at pre-sowing preparations. In all treatments the amount of nitrogen was the same, 140 kg N/ha. In 2010 at Humpolec site, CULTAN urea ammonium nitrate + inhibitor of nitrification treatment gave by 20.5% higher number of ears compared to CULTAN urea ammonium nitrate treatment. In 2011 at Ivanovice all CULTAN treatments reached statistically significantly higher number of kernels per ear. The higher 1000 kernel weight at CULTAN treatments was observed in 2012 at the Ivanovice site; a statistically significant difference between conventional and CULTAN urea ammonium nitrate + inhibitor of nitrification treatment was observed. Fertilization of maize with nitrogen using the CULTAN method under the conditions of the Czech Republic provides the same yield certainty as the conventional surface application and the CULTAN method of fertilization increases the yield certainty at delayed sowing. Harvest index was statistically significantly influenced by year, fertilization treatment and site.

The impact of non-treated and primed seeds on molasses components, sugar content and technological white sugar yield of the same cultivar of sugar beet root was investigated. The study was conducted in 2012-2014 at the Experiment Field Station of Warsaw University of Life Sciences - SGGW in Skierniewice (51°97'N, 20°19'E) in the central region of Poland. The use of primed seeds resulted in a higher technological white sugar yield with higher sugar content and lower content of α-amino nitrogen in the roots. Also, seed priming increased the technological value of the roots by a lower share in the root yield fractions of the root weight less than 300 g, characterized by lower sugar content and a higher content of α-amino nitrogen.

Within a pilot study, after pedological and mineralogical characterization, various kinetic models were tested to fit lead (Pb)-mobilization kinetics from soils at historic mining and smelting sites. Pb mobilization was obtained by modified electro-ultrafiltration (EUF) after addition of diethylenetriaminepentaacetic acid (DTPA) at variable conditions of extraction. 10 fractions were sequentially produced, under mild conditions at 20°C/200 V (to simulate an initial release) for fractions 1-5, and subsequently harder conditions at 80°C/400 V (to simulate a long-term release) for fractions 6-10. The special samples treated within this work yielded higher extraction rates within the first runs. Closest fits in terms of the coefficient of determination (R²) were obtained from the 2^nd order polynomial model y = a + bt + ct², and in terms of re-calculated results by the parabolic equation y = a + b √t. The fitted constants obtained by the modified EUF method correlated better with soil pH than with organic carbon and clay contents. From this, it remains open, whether the dissolution of the Pb-minerals in the electric field or concentration resp. diffusion of the DTPA is rate-determining.

An oven drying method was used to dry traditional Indonesian crackers, popularly known as kerupuk, applying drying temperatures of 50, 60 and 70°C and three different flavours, i.e., garlic, chili and seaweed. Newton, Page, Two terms, Midilli, Logarithmic and Henderson & Pabis mathematical models were used to fit the best model while the standard error of estimate (SSE), root mean square error (RMSE) and coefficient of correlation (r) were chosen as the criteria to determine the equation of the best fit drying model. The Midilli model was the best fit for all the kerupuk flavours. The effective moisture diffusivity was in the range of 1.0413 × 10^-10-1.6363 × 10^-10 m².s^-1 for the garlic flavour, 1.0007 × 10^-10-1.5619 × 10^-10 m².s^-1 for the chili flavour and from 1.0000 × 10^-10 to 1.6228 × 10^-10 m².s^-1 for the seaweed flavour. The activation energy of the garlic flavour, chili flavour and seaweed flavour kerupuk are 20.64, 20.64 and 22.57 kJ.mol^-1, respectively. Furthermore, in the present study, the physical properties, i.e., the colour and hardness of the kerupuk crackers after the drying process was investigated.

Patterns of biomass allocation were determined for seedlings of five provenances of Quercus castaneifolia from west to east of the Hyrcanian forest along a rainfall gradient. Experimental design was executed under controlled conditions at seven different light levels (10, 20, 30, 40, 50, 60, 70 and 100% full light). We quantified the biomass allocation patterns to leaves, stems and roots. For all provenances total mass increased with irradiance at low light levels, reaching an optimum at an intermediate level but decreasing at a high irradiance level. As results show, in drier provenances and at high light levels, the seedlings invest more biomass into root mass to facilitate water uptake and to alter their leaf size to prevent overheating. In contrast, at wetter provenances and low light levels, towards increased light interception, more biomass is allocated proportionally to leaves and the stems but, accordingly, less to roots. The leaf to root ratio (L/R) was negatively correlated with light, with high correlation at wetter provenances compared to drier ones. In contrast, the relationship between the root to shoot (R/Sh) ratio and light was positively correlated with light, but it was weak at drier provenances and became gradually stronger at wetter ones. Such relationships indicated that chestnut oak seedling growth strategies are different along a rainfall gradient to irradiance levels. Despite similar growth conditions in the greenhouse, different growth strategies may be the result of genetic adaptation to the ecological conditions, especially when precipitation regimes prevail in the native habitat.

The aim of the study was to determine the adaptive characteristics of pine needles associated with age and different growing conditions. The length of the needles decreases and its variability reduces with increasing dryness and poverty of the soil. In oppressed trees, the coefficient of variability of the length of the needles on the tree is 8%. The coefficient of variation in the length of needles approaching 20% will indicate the best conditions for the growth of a particular tree. Trends of the dependence of width and thickness of needles on growing conditions were not identified. The area of needles in pine forests with optimal water regime of soils (blueberry, cowberry type) varies in the range of 112-124 mm². In extreme growing conditions pine needles area is reduced by 27-33% and equals 76-86 mm². These ranges of values of the areas of needles are typical for plantings of the third and fourth classes of age. Changing the width and thickness of the needles is aimed at compensating for changes in the length of the needles in the direction of maintaining the optimal area for these conditions needles. In extreme conditions, the area of the assimilating tissue increases, and the area of the conducting tissue (stele) decreases. Correlation dependences of the area of the stele of needles with the cross-sectional area, with the area of conducting beams, with the number of resin canals and with the cover fabric are revealed.

The quantitative determination of four theaflavin monomers by a rapid reversed-phase high performance liquid chromatographic method was developed. A new RP-18 end-capped column with particle size 2 µm and equilibrated to 35°C in a Shimadzu temperature controller module was used. Four theaflavin monomers were successfully separated in 8 min by the new strategy, comparing to 20-85 min by HPLC in the peer literature reports. Linear gradient elution: from 92% mobile phase A (v) to 76% mobile phase A (v) during early 3 min and then 92% mobile phase A (v) till 8 min at elution flow rate 1.5 ml/min. The limits of detection and quantification were in the range of 0.1-0.3 and 0.4-1.1 mg/l. Satisfactory recoveries of theaflavin, theaflavin-3-gallate, theaflavin-3'-gallate and theaflavin-3,3'-gallate were 97.5-102.6, 98.6-102.4, 99.6-105.4, and 95.5-105.4%, respectively. The new method was applied to quantitative analysis theaflavins of tea samples, including 10 black teas, 5 oolong teas, and 5 green teas. This method is suitable for the rapid, accurate and inexpensive quantitative analysis of theaflavins under the basic detection conditions of HPLC.

Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments during which oocytes were repeatedly exposed to 2mM SNAP for a short time (10, 20 or 30 minutes) were more efficient with regard to the activation rate, even when the total exposure time did not exceed 4 hours. Parthenogenetic development was very limited after continuous treatment with 2mM SNAP. A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to the morula stage 0 vs. 18%; development to the blastocyst 0 vs. 7%; P < 0.05). However, this developmental rate was significantly lower (P < 0.05) than the development induced by conventional activation treatment with calcium ionophore (development to the morula stage, 23%; development to the blastocyst stage, 18%). When we combined pulsatile SNAP-treatment with the effect of protein kinase inhibitor 6-dimethyl aminopurine (6-DMAP) (2mM 6-DMAP for 2 hours) or with the inhibitor of protein synthesis cycloheximide (CHX) (10 µM CHX for 2 hours), we observed a significant increase (P < 0.05) in the activation rate when compared to the respective pulsatile SNAP-treatment without 6-DMAP or CHX (63 vs. 78% of activated oocytes for 6-DMAP; 63 vs. 83% of activated oocytes for CHX). However, the development of parthenogenetic embryos was not enhanced when the pulsatile SNAP-treatment was combined with 6-DMAP or with CHX.

One of the main issues addressed by phytology in recent years has been plant tolerance mechanisms for abiotic stress. No combined analysis has been made to identify the genes involved in drought stress tolerance. The meta-analysis of microarray data related to drought stress was analysed by the R software packages and showed 3 029 upregulated genes and 3 017 downregulated genes. The upregulated genes were mostly related to the drought tolerance protein, abiotic stress response, and the Cys2His2 Zinc Finger Transcription Factor (C2H2 zinc finger TF). The downregulated genes were mainly related to the late embryogenesis abundant protein, abiotic stress response, and the basic leucine zipper (bZIP) TF. The common gene ontology (GO) terms in the upregulated and downregulated genes were mainly related to the metabolic process, response to stimulus, cellular metabolic process, and photorespiration. The up and down meta-differential expressed genes (meta-DEGs) mainly belonged to the those following Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including: the biosynthesis of secondary metabolites, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signalling pathway, and RNA degradation. Moreover, in the upregulated and downregulated genes, the TFs with a high percentage mainly belonged to the Teosinte branched1/Cincinnata/proliferating cell factor (TCP), basic helix loop-helix (bHLH) and bZIP. Next, the hub upregulated genes were mainly related to the thiamine biosynthesis protein thiC, 4-hydroxyphenylpyruvate dioxygenase, ribose-5-phosphate isomerase precursor and heat shock protein. The hub downregulated genes were mainly associated with the elongation factor Ts, aldehyde dehydrogenase, and trigger factor. Finally, the data from the present meta-analysis were compared with previous studies on the qRT-PCR results and their up and down expressions were confirmed. Based on the findings of the current study, novel insights into the drought stress molecular response can be provided and various candidate genes can be introduced for barley drought stress tolerance breeding.

Conventional irrigation practices result in a substantial amount of water loss with okra cultivation. Although micro-irrigation can address this issue by delivering water directly near the rootzone, it requires manual operation. These issues, however, can be resolved with the introduction of a smart micro-irrigation system. This study aims to develop a smart micro-irrigation system for okra, in conjunction with the sub-components of drip irrigation, a microcontroller, and a soil sensor. The experiment was laid out with a randomised complete block design (RCBD) having three treatments: (i) control irrigation (T₁), (ii) drip irrigation (T₂), and (iii) smart micro-irrigation (T₃). The experimental field was irrigated based on soil moisture regimes in the crop rootzone. The plant growth, yield, and water use efficiency were assessed to evaluate the system. The results showed no significant differences among these treatments (at P < 0.05). The best water usage efficiency (15.98 kg·m^–3) was observed in the T₃ treatment, which also provided about 13.10% water savings compared to the conventional irrigation. This study indicates that a smart micro-irrigation system could be a promising technology for water-efficient okra cultivation.

The aim of this work was to study the use of different types of wood products by the G20 countries. In the current scenario, every country is concerned about the severe exploitation of forests and the ensuing lack of markets for timber. Production and consumption of wood and wood products by the G20 countries account for 85% of global GDP and two-thirds of the world's population. Data for the study includes eight major categories of wood products in the G20 countries from 2004 to 2021: industrial raw wood, sawlogs and veneer logs, sawn wood, wood fuel, wood-based panels, paper and paperboard, pulp for paper, wood pellets, and other agglomerates. Autoregressive integrated moving average (ARIMA) models were used to predict the production, export, and import behaviour of wood and wood products from 2022 to 2030. According to the findings, the G20 countries would consume 1 644.6 million m³ of industrial raw wood in 2030, followed by 984.8 million m³ of sawlogs and veneer logs, 454.7 million m³ of sawn wood, 882.5 million m³ of wood fuel, 406.2 million t of paper and paperboard, 199.4 million t of pulp for paper, and 51.7 million t of wood pellets and other agglomerates. This study provides decision-makers with an analysis of the production and consumption of wood product markets in the G20 countries, which can be used for policy decisions.

In this study, the influence of microclimatic conditions in different seasons of the year (including the hottest months) on the quantitative and qualitative characteristics of ejaculates of Czech Holstein bulls was assessed. Bulls were kept in the facility with no forced ventilation or air conditioning. To determine the influence, the temperature-humidity index (THI) was calculated based on the microclimatic parameters measured directly in the facility with the animals. Bull sperm was obtained using an artificial vagina on five occasions throughout the year, in different seasons of the year including the hottest months. Semen was assessed for its volume, sperm concentration, and motility by CASA, as well as cellular viability by using flow cytometry. In the present study no statistically significant positive correlations between values of THI and sperm degradation were observed. Obtained preliminary results give grounds to believe that Czech Holstein bulls produced high-quality sperm during the summer, the hottest months of the year.

Staphylococcus aureus is a zoonotic pathogen that can cause various diseases in both humans and animals. This experiment evaluates the antibacterial activity of extracts from Acer truncatum leaves against S. aureus, including the minimum inhibitory concentration (MIC), growth curve, and cell membrane integrity assessment, alongside the identification of the extract components by LC-MS. Results demonstrated that the MIC of the n-butanol extract from the ethanol extract of Acer truncatum leaves against S. aureus was 3.125 mg/ml, with a minimum bactericidal concentration (MBC) of 6.25 mg/ml. Over 16 h, the extracts at concentrations of 0.25 MIC, 0.5 MIC, and 1 MIC effectively inhibited S. aureus growth. The fluorescence staining revealed that the extracts at different concentrations compromised the structural integrity of the cell membrane. The LC-MS analysis identified the principal constituents of the extract as betaine (27.189%), 3,4-dihydroxymandelic acid (16.112%), quercitrin (14.768%), chlorogenic acid (8.778%) and neochlorogenic acid (4.452%). The study indicated that Acer truncatum leaf extract has good potential for application in natural antibacterial drugs.

This study elucidates the expression dynamics and biological functions of oar-miR-214_3p in ovarian and uterine tissues of Liangshan Black Sheep across distinct stages of the oestrous cycle, providing novel insights into its regulatory mechanisms governing ovine reproductive physiology. The study employed RT-qPCR, northern blotting, FISH, dual-luciferase reporter assays, and ELISA to analyse the expression and biological functions of oar-miR-214_3p in the ovary and uterus of Liangshan Black Sheep across different stages of the oestrous cycle (proestrus, oestrus, metoestrus, and dioestrus phases). RT-qPCR and northern blotting revealed that oar-miR-214_3p is expressed in the ovarian and uterine tissues, with significant variations across different stages. Compared with the dioestrus and metoestrus phases, oar-miR-214_3p expression was significantly increased during the proestrus and oestrus phases (P < 0.05), with the highest levels observed during oestrus (P < 0.05). FISH analysis indicated that oar-miR-214_3p is primarily localised in the cytoplasm. We constructed wild-type and mutant vectors for mammalian target of rapamycin(mTOR) and Semaphorin 4D (Sema4D). After intervention for 48 h in granulosa cell cultures, RT-qPCR analysis of mTOR and Sema4D expression revealed that the mimic and inhibitor groups suppressed and promoted the expression of these target genes, respectively. The control and NC groups showed stable expression levels with no significant differences. Dual-luciferase reporter assay confirmed that mTOR and Sema4D are oar-miR-214_3p target genes. ELISA revealed that the mimic and inhibitor treatment groups promoted and suppressed oestrogen secretion, respectively. These findings confirm that oar-miR-214_3p, primarily localised in the cytoplasm, regulates ovarian follicle development, ovulation, oestrogen secretion, and the oestrous cycle via its target genes, mTOR and Sema4D. The study provides valuable insights into improving sheep reproductive performance through molecular breeding techniques. These findings have significant clinical and practical applications for enhancing reproductive efficiency.

The sustainable management of European forest ecosystems necessitates innovative mechanisation solutions to address operational challenges in hilly, mountainous, and ecologically sensitive terrains. Hybrid-drive unmanned vehicles (HDUVs) present a transformative potential by integrating fuel-electric powertrains with autonomous navigation systems, enabling energy-efficient operations with minimal environmental impact. This review synthesises and critically analyses advancements in three critical domains: (i) dynamic modelling and chassis design for enhanced terrain adaptability, (ii) hybrid powertrain optimisation for reduced emissions and extended operational range, and (iii) the integration of unmanned systems for precision forestry tasks. By examining multi-body dynamics, power management strategies, and AI-driven navigation algorithms, we elucidate the role of HDUVs in improving operational efficiency while mitigating soil disturbance and carbon footprint. The review identifies prevailing research gaps and suggests that future work should prioritise the development of standardised testing protocols and foster cross-disciplinary collaboration to align HDUV development with EU biodiversity and climate objectives.

This study aimed to comprehensively evaluate the prevalence, severity, and risk factors associated with gastric lesions in fattening pigs across all four seasons in Slovakia. A total of 1 944 porcine stomachs were examined post-mortem at commercial slaughterhouses, focusing on the non-glandular region (pars oesophagea). A macroscopic evaluation was conducted using a standardised scoring system (0–3), in which gastric lesions, including parakeratosis, erosions, and ulcerations, were observed in 48% of the examined stomachs. Significant seasonal variation was detected, with the highest lesion prevalence recorded during the summer months, likely due to heat stress and reduced feed intake, and the lowest incidence of pathological changes seen in autumn. The gastric fullness had a notable impact: empty and liquid-filled stomachs were more frequently associated with severe mucosal damage, while full stomachs exhibited a protective effect. Furthermore, the feeding regimen played a crucial role: the pigs receiving wet feed had a significantly lower prevalence of gastric lesions than those on a dry feeding regimen. These results underscore the multifactorial nature of gastric ulceration in pigs and highlight the importance of nutritional and environmental management strategies in intensive production systems.

Xinjiang apricot is favoured by consumers because of its distinctive aroma, high nutritive value, and abundant functional active substances. Carotenoids of apricot are efficient antioxidants that can protect the human body from free radical attack. However, the extraction, quantification, and antioxidant activity of carotenoids from Xinjiang apricots have not been reported. In this work, ultrasound-assisted ionic liquid (ILs) extraction and optimisation of carotenoids from Xinjiang apricots and to evaluate their antioxidant potential. Based on Box–Behnken design (BBD), the best conditions were IL/ethanol (R_IL/E) ratio of 1 : 2, solid-liquid ratio (R_S/L) of 1 : 3, extraction time of 17 min and number of extractions of 3. The content of carotenoid extracted by ultrasonic-assisted [Bmim][BF₄] ILs was 32.98 ± 0.27 μg·g^–1 that of traditional extraction method was 25.05 ± 0.35 μg·g^–1. Moreover, ultrasonic-assisted ILs extraction technology can shorten the extraction time, simplify the extraction steps and increase the extraction amount. Meanwhile, in order to recover and reuse ILs, ILs-ethanolic solution was frozen at temperatures lower than –80 °C, allowing the ILs precipitation and separation from the ethanol solution. Meantime, the antioxidant potential of five Xinjiang apricot varieties were evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] assays in vitro and analysed by UV–vis spectroscopy and Raman spectroscopy. The results showed Shushanggan apricot has the highest carotenoid content and the strongest antioxidant activity. In conclusion, this research further proves the advantage of ultrasonic-assisted ILs in carotenoid extraction and the potential to obtain valuable carotenoids from the apricot industries.

In this study, volatile compounds from Cinnamomum cassia Presl. leaves from different regions of China were identified using gas chromatography coupled with mass spectrometry (GC-MS) and Fourier-transform infrared (FTIR) spectroscopy combined with chemometrics. The results showed that the essential oil yields greatly varied across regions, with the density of oil cells at the accumulation and saturation stages playing a key role in this yield. GC-MS analysis revealed a higher content of trans-cinnamaldehyde in samples from the Xijiang River basin (No. 1–8) than in those from Baise Guangxi (No. 9). Variable importance in projection analysis identified five differential marker components for assessing the geographical origin of C. cassia leaves: trans-cinnamaldehyde, acetophenone, cis-cinnamaldehyde, camphor, and α-thujene. Hierarchical cluster analysis, similarity evaluation, and principal component analysis from FTIR fingerprinting indicated that essential oil compositions of samples No. 1–6 from the Xijiang River basin were closely related. In contrast, the Baise sample (Western Guangxi) significantly differed from the other eight, likely due to the geographical distance. Our results indicate that the methods employed are effective for determining the geographical distribution and assessing the quality of raw cinnamon in herbal medicine.

This review aims to enrich our understanding of Chiari-like malformation (CLM) by combining human and veterinary insights, and providing a detailed cross-species overview. CLM is a developmental abnormality characterised by caudal displacement of the hindbrain into the foramen magnum due to an entire brain parenchymal shift caused by insufficient skull volume. This malformation leads to a progressive obstruction at the craniocervical junction, which disrupts the normal cerebrospinal fluid flow, leading to secondary syringomyelia. The clinical signs of CLM and syringomyelia include phantom scratching, head tilt, head tremor, ataxia, tetraparesis, pain, muscle atrophy, and scoliosis or torticollis. Magnetic resonance imaging remains the gold standard for diagnosing CLM, since it allows the visualisation of abnormal findings such as the caudal cerebellar herniation, caudal cerebellar compression from occipital dysplasia, and attenuated cerebrospinal fluid cisternae. Although various medical and surgical interventions, including foramen magnum decompression, can provide temporary symptomatic/clinical sign relief, current literature shows a lack of sustained long-term efficacy. Therefore, additional research is needed to evaluate the long-term effects of existing treatment strategies and to compare different techniques utilised in conjunction with foramen magnum decompression.

Chilli anthracnose, incited by Colletotrichum capsici, is a major disease affecting the quality and quantity of chilli production. Farmers greatly depend on synthetic fungicides for the management of the disease. However, the extensive and non-judicious use of chemical fungicides resulted in the development of fungicide resistance in the pathogen and associated human and animal health risks. Piriformospora indica, a beneficial fungal root endophyte, has been employed as an efficient and safe biocontrol agent for managing bacterial, fungal and viral diseases and enhancing growth and yield. Hence, the present study was carried out to establish the protective role of P. indica against the chilli anthracnose incitant, C. capsici. The enzymes of phenylpropanoid pathway involved in this tripartite interaction were also studied. The study demonstrates that P. indica restricted C. capsici growth in dual culture with 57.22% mycelial inhibition on the 15th day after inoculation. P. indica ‒ colonized chilli plants showed a delay in disease development, and significantly reduced the incidence and severity of chilli anthracnose disease compared to the control plants. Higher activities of defence-related enzymes viz. peroxidase, phenylalanine ammonia-lyase, polyphenol oxidase, 4-coumaryl CoA ligase, cinnamyl alcohol dehydrogenase and total phenol in the P. indica ‒ colonised plants revealed that the endophyte early resistance of plants against further pathogen invasions. The present study revealed P. indica to be an efficient biocontrol agent against chilli anthracnose. The results showed that P. indica reduced the infection of C. capsici by direct antagonism, activation of enzymes involved in plant defence and enhanced growth in chilli plants.

In this study, we examine the effect of Agricultural Socialisation Services (ASS) on green grain production efficiency in Jiangsu Province, China, by using data from the China Land Economy Survey. We used the generalised random forests model in this research to address potential issues of farming household self-selection into ASS and unobserved heterogeneity in treatment effects. The results show that participation in ASS significantly improves green production efficiency, particularly for small-scale farmers. Efficiency gains are most pronounced in critical agronomic operations such as pest control, seeding and planting, whereas smaller efficiency effects are observed in plowing, harvesting and straw treatment. The findings suggest that targeted expansion of ASS could substantially enhance sustainable farming practices, especially for resource-constrained farms. This study provides important policy insights for promoting agricultural sustainability through improved access to and delivery of agricultural services, contributing to more efficient and ecofriendly grain production.

The influence of spermidine (Spd) on wheat ascorbate and glutathione metabolism, copper (Cu) accumulation and photosynthetic performance under Cu stress was studied. The findings displayed that Cu stress boosted reduced ascorbate (AsA) and reduced glutathione (GSH) contents by improving ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), L-galactono-1,4-lactone dehydrogenase (GalLDH) and gamma-glutamylcysteine synthetase (γ-ECS) activities. Nevertheless, Cu stress promoted malondialdehyde (MDA) accumulation and electrolyte leakage (EL) level, and lowered AsA/dehydroascorbic acid (DHA) and GSH/oxidised glutathione (GSSG). Meanwhile, Cu stress promoted Cu accumulation in plant tissues. It declined net photosynthetic rate (Pn), chlorophyll fluorescence parameter maximum photochemical efficiency of PSII (Fv/Fm), chlorophyll (Chl) and carotenoids (Car) contents, and wheat height and biomass. In this way, Cu stresses limited wheat growth. Compared with Cu stress, Spd plus Cu stress enhanced APX, GR, DHAR, MDHAR, GalLDH and γ-ECS activities to 4.75, 5.14, 3.77, 2.96, 3.24 and 2.83 U/g FW (fresh weight), respectively. This way, Spd further increased AsA and GSH contents to 4.62 and 0.78 µmol/g FW under Cu stress. Meanwhile, Spd increased AsA/DHA to 14.60 and GSH/GSSG to 15.97 and declined MDA content to 11.68 nmol/g FW and EL to 17.00% under Cu stress. Besides, Spd declined Cu content in leaves to 68.8 µg/g DW and roots to 152.9 µg/g DW and respectively increased Pn, Fv/Fm and Chl and Car contents to 15.22 µmol/m2/s, 0.74, 1.55 mg/g FW and 0.38 mg/g FW. In this way, Spd promoted wheat growth under Cu stress. Meanwhile, we found that Spd alone also improved the ascorbate and glutathione metabolism, photosynthetic performance, and wheat growth compared to the control. These results illustrated that Spd mitigated wheat Cu toxicity by reducing Cu accumulation and improving ascorbate and glutathione metabolism and photosynthetic performance. Hence, using Spd will be a good strategy to improve the Cu tolerance of wheat crops in the future.

An unprecedented bark beetle outbreak has led to a significant decline in forest cover in Central Europe in the last 10 years, affecting an area estimated at more than 200 000 ha in the Czech Republic. Among the many ecological threats associated with extensive clearings, the potential alteration of hydrological processes is one of the most important. Therefore, after 2022, the precipitation–runoff balance in three catchments in the Jeseníky Mts. area was studied. This study focuses on the rainfall variability within the area, which was measured using 24 rain gauges deployed to cover different altitudes as well as the geographical exposures of the mountain catchments. Precipitation data was evaluated based on seven-day totals within the frost-free period. There was a significant increase in precipitation with altitude (12% increase for every 100 m a.s.l.) but only in less than half (48.7%) of the evaluated periods. No significant trend was demonstrated in the remaining periods, and a negative trend in precipitation with altitude was identified in 8.9% of cases. Additionally, the role of exposure was insignificant, although a tendency towards slightly lower precipitation was found for the eastern exposure at altitudes up to 1 000 m a.s.l., which may be related to the prevailing wind direction. We concluded that even a relatively dense monitoring network is not necessarily sufficient to provide accurate precipitation data in forested catchments, especially in mountain areas. Under such conditions, the use of complex models that also use radar data is recommended.

A root detector is a non-destructive technology developed to indicate the radial distribution of tree roots, which are not often visible on the surface. This study aims to assess the accuracy of the root detector in estimating the radial distribution of both exposed and buried tree roots. Six Agathis loranthifolia Salisb. trees were selected, three with exposed roots and three with buried roots. The Fakopp® root detector, an acoustic-based tool, was used in this study. Root estimation was based on a combination of threshold values (> 400 m·s^–1), average values, and the peak of the sound wave velocity. Soil excavation was manually conducted at a depth of 30 cm within a 100 cm radius of the tree trunk. The results showed that under similar soil conditions, the root detector achieved an accuracy of over 80% in detecting the actual radial root distribution, as validated by the excavation method. Root diameter exhibited the strongest correlation with sound velocity in detecting lateral roots. However, root depth and inclination angle contributed to detection inaccuracies in estimating the radial distribution of lateral roots.

The testing and evaluation of native populations of perennial ryegrasses (Lolium perenne L.) collected from the Republic of Srpska, Bosnia and Herzegovina were conducted from 2020 to 2022 at experimental fields and laboratories of the Institute for Genetic Resources and the Agricultural Institute of the Republic of Srpska in Banja Luka. Six native populations (labelled G1–G6) and two cultivars, Esquire and Tivoli (labelled G7 and G8), were analysed in this study. The following traits were examined: the plant height (cm), number of generative stems per plant, inflorescence length (cm), number of spikes per inflorescence, seed mass per inflorescence (g), and seed yield per plant (g). The results showed that all the native populations exhibited higher average plant heights compared to the Esquire standard (G7). Population G2 had the highest average number of spikes per inflorescence (26.8), while population G4 achieved the greatest average inflorescence length (27.7 cm). Statistically significant differences in the inflorescence length were observed between populations G2, G3, G4, and G6, compared to the standards G7 and G8 (Esquire and Tivoli). The genotype and year had a significant impact on the seed yield per plant. The average yield ranged from 32.4 g (G7) to 53.4 g (G4). The seed yield per plant showed a strong positive correlation with the number of generative stems per plant, the inflorescence length, and the number of spikes per inflorescence. The native populations G4 and G5 achieved significantly higher seed yields per plant compared to the other populations and may serve as highly valuable material for breeding programmes.

This research explores the addition of mangrove (Sonneratia alba) fruit to reduce the production of methane and the total population of protozoa. The dosage for adding mangrove fruit is 0% (without addition), 1.5%, 3%, and 4.5% in sugarcane tops-based feed. Results include ruminal product fermentation, gas and methane emissions, total protozoa, microbial protein production, microbial biomass, and nutrient digestibility. The research findings showed that an additional 1.5% to 4.5% dose can reduce methane gas emissions and the total number of protozoa. The total number of protozoa at 4.5% (T3) reached 9.89 × 10⁴ cells/ml and methane gas was 56.1 ml/g DM (dry matter); 8.41 ml/g OM (organic matter). This effect is attributed to the tannin content in mangrove fruit, which exhibits antimicrobial properties. However, increasing doses also reduced nutrient digestibility. The findings suggest that incorporating 1.5–4.5% mangrove (Sonnetaria alba) fruit as a source of tannins causes a positive impact which reduces protozoa populations and methane production without changing the ruminal fermentation product. However, the addition of mangrove fruit in this study caused also reduced nutrient digestibility.

As the strategy for building a robust agricultural nation gains momentum and agricultural science and technology advances, the quality of agricultural products has seen significant improvement, accompanied by an increase in the economic income of agricultural producers and operators. Therefore, the fair and reasonable implementation of the revenue distribution of the agricultural supply chain is of great significance in improving the quality of agricultural products and ensuring the stable operation of the supply chain. The article focuses on the three main bodies of the agricultural supply chain, namely production and price co-integration enterprises, logistics service enterprises and sales enterprises, and utilises the matrix semi-tensor product to establish the Shapley value revenue allocation model of the interval cooperation game, so as to make the revenue allocation of the governance of agricultural products' quality in the digital supply chain more reasonable and scientific. Finally, numerical examples verify the Shapley value model, demonstrating that this revenue allocation scheme, when applied, can boost the overall supply chain's revenue through cooperative agricultural product quality management, elevate agricultural product quality and market competitiveness, and foster collaboration to ensure the stability of supply chain operations.

This paper analyses the accounting and tax treatment of forests as biological assets in the Czech Republic under Act No. 563/1991 Coll., on Accounting, and its implementing regulations. It compares national approaches with the International Financial Reporting Standards (IFRS), focusing on the International Accounting Standard IAS 41 – Agriculture. A comparative-analytical method was used to identify key differences in classification, valuation, and reporting practices. Czech legislation values forests at historical cost and classifies them as non-depreciable land, whereas IFRS requires fair value measurement, capturing biological transformation and the economic potential of forest ecosystems. The findings show that while the Czech framework ensures legal clarity and tax alignment, it lacks integration with environmental accounting frameworks such as the United Nations System of Integrated Environmental Economic Accounts (SEEA) and does not reflect ecosystem services or ESG (environmental, social, governance) reporting needs. In contrast, IFRS provides a dynamic representation of forest assets, supporting transparency and comparability in international contexts. The study contributes to the ongoing harmonisation debate by offering recommendations to improve the alignment of Czech accounting with international standards, thus supporting sustainable forest management and enhancing investment credibility. Its originality lies in combining legislative analysis with international practice review, highlighting the gap between ecological value and financial reporting in forestry.

A free electricity tandem-twin-hybrid-solar-biomass dryer comprised of two drying rooms and operated with solar and biomass energy combustion of 10 kg rubber wood per hour separately to dry Robusta coffee cherries with 3, 6, 9, and 12 cm bed thicknesses were studied with the drying completion time (tc), number of defects (ND), and colour parameters, i.e., lightness (L*), hue angle [H(^o)], and chroma (C), used as the performance indicators. The experimental results indicated that the drying room, bed thickness, and drying room-bed thickness interaction significantly affected the tc and ND and bed thickness only significantly affected C for both the solar energy drying and the biomass energy drying. The solar energy drying generated a drying air temperature of 44.6 ± 3.5 °C with a tc of 70.9–90.2 h for the front drying room and 40.1 ± 2.8 °C with a tc of 77.2–116.5 h for the rear drying room, whereas the biomass energy drying produced a drying air temperature of 57.2 ± 3.6 °C with a tc of 34.1–44.9 h for the front drying room and 45.6 ± 6.0 °C with a tc of 56.3–96.6 h for the rear drying room. Both drying processes produced coffee beans with the NDs less than 11 qualified for Grade 1 with similar colour characteristics.