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Results 871 to 900 of 5763:

Anaerobic bio-processing of agricultural waste for the biotechnological production of lactic acid and volatile fatty acid by landfill soil inoculumsOriginal Paper

Andriy Anta Kacaribu, Darwin Darwin, Yuliani Aisyah, Febriani

Res. Agr. Eng., 2025, 71(1):10-21 | DOI: 10.17221/52/2024-RAE


With the increase in the world population and the ensuing surge in organic waste, effective management strategies are crucial to prevent environmental pollution. This study aims to address this challenge by utilising organic waste (OW) as the substrate for the production of lactic acid (LA) and volatile fatty acids (VFAs) through anaerobic bioprocessing. The substrates used, included grass, starch, and fruit wastes inoculated with non-sterile inoculum landfill soil (LS). The anaerobic bioconversion was performed by varying the substrate to the inoculum. The results unveil that a digester loaded with 150 g·L–1 of fruit waste, exhibits the highest concentration of LA, reaching a significance of 25 mmol·L–1. A digester fed with 100 g·L–1 starch, also manifests significant LA production (18.50 mmol·L–1). A digester, supplied with 150 g·L–1 starch waste, showcases the highest VFA (92.5 mmol·L–1). Intriguingly, the anaerobic bioprocessing of the grass substrate did not produce LA at all, yet al. the substrates showcased VFA production, albeit with fluctuating and lower concentrations. This study highlights the potential of incorporating simple sugar for enhanced LA production and starch-based substrates for increased VFA production when utilising LS as the inoculum. The anaerobic bioprocessing shows promising outcomes for the future development in sustainable waste utilisation.

The effects of zinc oxide nanoparticles on the oxidative stress, caspase-3, cytokine and immunity in ratsOriginal Paper

M Gultekin, M Eren, FD Koca, CK Bozbek, N Develi

Vet Med - Czech, 2025, 70(10):379-392 | DOI: 10.17221/11/2025-VETMED

This study was conducted to examine the effects of zinc oxide nanoparticles (ZnO NPs) on the malondialdehyde (MDA) concentrations, superoxidase dismutase (SOD), glutathione peroxidase (GPx) and caspase-3 (CASP3) activities, tumour necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), immunoglobulin (Ig) E, G, M and zinc (Zn) concentrations in the serum/plasma and liver tissues of rats. Forty Wistar Albino rats were separated into five equal groups as the control, 5 and 10 mg/kg, b.w./day ZnO, 5 and 10 mg/kg b.w./day ZnO NPs were administered i.p. every other day for 14 days. The plasma MDA and plasma/liver TNF-α concentrations increased in the 10 mg/kg ZnO, 5 and 10 mg/kg ZnO NPs groups. The plasma SOD, CASP3, plasma/liver GPx activities and serum Zn concentrations increased in all the Zn groups. The highest SOD, GPx and CASP3 activities were detected in the 5 mg/kg ZnO NPs group. The plasma IgG concentrations increased, especially in the ZnO NPs groups. The study findings suggest that 5 mg/kg ZnO NPs could potentially have an ameliorative effect on the possible adverse effects of oxidative stress. These nanoparticles demonstrate their ability to combat oxidative stress by increasing the plasma/tissue SOD, GPx, and CASP3 activities, TNF-α, and IgG concentrations. However, the effectiveness of the nanoparticles may vary depending on the synthesis method, application time, and concentration.

Crystallinity of starch, food composition, and digestibility of starchReview

Evžen Šárka, Petra Smrčková, Marcela Sluková

Czech J. Food Sci., 2025, 43(2):90-104 | DOI: 10.17221/33/2025-CJFS

Starch granules in their native state are insoluble and semi-crystalline. There are three forms of starch/amylopectin in nature: A, B, and C: in cereals (A), tubers and high amylose starches (B), and some varieties of peas and beans (C). Crystallinity and rate of starch hydrolysis depend on the plant species and growing conditions. The changes during food preparation include gelatinisation, the formation of amylose-lipids and amylose-protein complexes, and resistant starch (RS) origin. They are accompanied by changes from crystal to amorphous form and vice versa. Starch in human food is mostly rapidly or slowly digestible. Rapidly digestible starch is formed by gelatinisation, cooking extrusion, breaking down of starch granules, or hydrolysis to maltodextrins. By definition, RS is not digested in the small intestine. This review addresses the influences of biochemical processes in the human body on starch digestibility. It is strongly influenced by the degree of chewing, the activity of α-amylase in the intestine, and transit time through the stomach and small intestine. Resistant starch and endogenous intestinal mucus support the growth of specialist microbes in the large intestine that produce a variety of short-chain fatty acids, causing the perception of satiety, lowering pH, and inhibiting pathogens in the colon.

Carcass traits and meat quality in Balkan goats: A multivariate evaluation of crossbreeding and slaughter weight effectsOriginal Paper

Nikola Stanišić, Bogdan Cekić, Nevena Maksimović, Maja Petričević, Nikola Delić, Slaviša Stajić, Tanja Keškić

Czech J. Anim. Sci., 2025, 70(10):438-449 | DOI: 10.17221/53/2025-CJAS


This study investigated the effects of slaughter weight and breed on carcass characteristics, meat quality, and technological properties in purebred Balkan and F1 crossbred (Balkan × Saanen) male goats. A total of 48 animals, divided into four slaughter weight groups (10, 15, 18, and 22 kg), were analysed for carcass composition, muscle traits, and fat distribution. ANOVA and multivariate analyses (PCA and HCA) revealed significant effects of weight and breed on key carcass and meat quality parameters. Heavier animals exhibited higher intramuscular fat and primal cut yields with superior water-holding capacity (WHC), while lighter goats had leaner carcasses with less muscle pigment. Crossbred F1 goats demonstrated a more balanced muscle-to-fat ratio, whereas pure Balkan goats tended to accumulate fat more rapidly at higher weights. These findings indicate that optimal slaughter weight selection should align with market demands, such as producing leaner meat in lighter Balkan goats, or achieving increased marbling and better economic returns in heavier crossbred animals. Future research should explore genetic effects on muscle and fat deposition and assess consumer preferences to refine breeding and production strategies.

Geographical variations in soil properties and bacterial community diversity across major lavender (Lavandula angustifolia Mill.) cultivation regions in the Ili River ValleyOriginal Paper

Junnan Jian, Shimin Tang, Shengjun Zhang, Ling Zhu, Bian Ran, Xuechao Zhang, Weikang Luo, Shilei Dong

Plant Soil Environ., 2025, 71(11):805-819 | DOI: 10.17221/377/2025-PSE

The Ili region hosts China’s largest lavender cultivation base, yet soil bacterial diversity in its primary cultivation areas remains understudied. To address this, we compared soil bacterial communities across four major cultivation counties (Chabuchar, Agricultural Research Institute, Yining, and Huocheng). Essential oil profiles, soil properties, and bacterial community characteristics were analysed to elucidate microbial variations and environmental interactions. The results showed that: (1) The essential oil yield (1.14%) and linalool content (41.04%) in the Huocheng County cultivation area were significantly higher than those in other areas, and the essential oil quality was relatively the best; (2) the soil bacterial communities in different main cultivation areas shared certain commonalities. At the phylum level, Proteobacteria, Acidobacteriota, Gemmatimonadota, and Actinobacteriota were the dominant phyla, and their relative abundances varied by region and soil layer, and (3) the redundancy analysis results showed that soil bacterial communities were comprehensively affected by environmental factors such as pH, total nitrogen, total phosphorus, soil organic carbon, longitude, and altitude. The significant positive correlations between the abundance of Vicinamibacteraceae (Acidobacteriota) in Huocheng County soils and both soil total phosphorus and linalool content suggest a putative mechanism whereby this bacterial taxon enhances lavender terpenoid synthesis by facilitating phosphorus cycling. Overall, these results suggest that geographically driven climatic variations dynamically alter the soil bacterial community, thereby influencing lavender growth and the final essential oil quality.

Study on the geographical origin and characteristic differential components of Qianbei Ma lamb based on rapid evaporative ionization mass spectrometryOriginal Paper

Ji Zhang, Rui Hou, Yuhang Luo, Xiaohong Zhang, Hong Luo, Lei Ling, Liping Xiang

Czech J. Food Sci., 2025, 43(1):59-70 | DOI: 10.17221/159/2024-CJFS

A rapid evaporative ionization mass spectrometry (REIMS) method combined with intelligent knife (iKnife) method was developed to explore the geographical origin and characteristic differential components of Qianbei Ma lamb. The REIMS conditions were initially refined, with the cauterization duration of 3 seconds, and the auxiliary solvent flow rate set to 100 μL·min–1 to prevent duplication. A database model was created from raw data through the proposed principal component analysis-linear discriminate analysis (PCA-LDA) in Live ID software, successfully applied to identify samples from 5 provinces in China and the real-time reliable identification rate with confidence higher than 99%. The obtained data by REIMS were used to establish the multivariate statistical models which using orthogonal projection to latent structures discriminant analysis (OPLS-DA), provided strong the discrimination power between composition and content changes of 16 specific ions such as m/z 726.3952 and m/z 744.4050, etc., including fatty alcohols, fatty acids and phosphatidylserines in mutton of different origin and the model displayed validation [R2(Y) = 0.968, Q2 = 0.924].

Proposal of a conceptual framework for creating a model for the implementation of green growth indicators in the conditions of the Slovak RepublicOriginal Paper

Samuel Fekiač, Iveta Hajdúchová, Roman Dudík, Kim Hortenská, Martin Halász

J. For. Sci., 2025, 71(8):373-383 | DOI: 10.17221/22/2025-JFS

Green growth promotes sustainable economic development by protecting the environment and efficiently using natural resources. Supporting green growth enhances long-term sustainability, quality of life, and climate change mitigation. This article aims to propose a conceptual framework for implementing green growth indicators in Slovakia by comparing their perception with that of the Czech Republic. A questionnaire survey was conducted among 3 281 Slovak and Czech forestry enterprises [Nomenclature of Economic Activities (NACE) classification 02.10], with 1 365 responses. The findings highlight significant differences in the approach to green growth indicators between small and large enterprises and between state and non-state enterprises. Additionally, the study examines how Slovakia and the Czech Republic manage these indicators, forming the basis for a conceptual framework for their implementation in Slovakia. The research contributes to the discourse on green growth and sustainability, key concepts in the 21st century.

Assessing essential oil composition in Cinnamomum cassia leaves from different regions of China using GC-MS and FTIR spectroscopyOriginal Paper

Yan Huang, Yujie Liu, Huiping Tan, Yanrong Cheng, Kunyang Tao, Dingze Gu, Huaizu Cai, Chengjie Li, Kaiyi Guo, Cheng Wu, Hong Wu, Yanqun Li

Czech J. Food Sci., 2024, 42(3):151-152 | DOI: 10.17221/197/2023-CJFS

In this study, volatile compounds from Cinnamomum cassia Presl. leaves from different regions of China were identified using gas chromatography coupled with mass spectrometry (GC-MS) and Fourier-transform infrared (FTIR) spectroscopy combined with chemometrics. The results showed that the essential oil yields greatly varied across regions, with the density of oil cells at the accumulation and saturation stages playing a key role in this yield. GC-MS analysis revealed a higher content of trans-cinnamaldehyde in samples from the Xijiang River basin (No. 1–8) than in those from Baise Guangxi (No. 9). Variable importance in projection analysis identified five differential marker components for assessing the geographical origin of C. cassia leaves: trans-cinnamaldehyde, acetophenone, cis-cinnamaldehyde, camphor, and α-thujene. Hierarchical cluster analysis, similarity evaluation, and principal component analysis from FTIR fingerprinting indicated that essential oil compositions of samples No. 1–6 from the Xijiang River basin were closely related. In contrast, the Baise sample (Western Guangxi) significantly differed from the other eight, likely due to the geographical distance. Our results indicate that the methods employed are effective for determining the geographical distribution and assessing the quality of raw cinnamon in herbal medicine.

Determination of resistance improving potentials of cotton whitefly Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) biotypes against cyantraniliproleOriginal Paper

Isse Hassan Ali, Utku Yükselbaba

Plant Protect. Sci., 2025, 61(2):191-200 | DOI: 10.17221/112/2023-PPS

Bemisia tabaci is a significant insect pest that causes extensive agricultural damage. The MEAM1 (Middle East-Asia Minor) and MED (Mediterranean) genetic groups of B. tabaci are the most prevalent cryptic species. This study investigated the potential for resistance development in the MED and MEAM1 genetic groups of B. tabaci against cyantraniliprole. Additionally, multiple-resistance development within each genetic group for pyriproxyfen, spirotetramat, and acetamiprid insecticides was determined. To assess the susceptibility of the first-larval stage of B. tabaci, we employed a systemic uptake method with cyantraniliprole. Additionally, we utilized a leaf-dipping method to apply acetamiprid, spirotetramat, and pyriproxyfen to second-instar larvae, adults, and eggs of B. tabaci, respectively, to determine the LC50 values for each insecticide. Each genetic group was subjected to six selections using cyantraniliprole. After six rounds of selection, a 1.8-fold resistance was observed in the B. tabaci MEAM1 population, whereas the MED population exhibited a 1.4-fold resistance. While B. tabaci MED and MEAM1 genetic groups exhibited very low resistance to cyantraniliprole, it's important to note that no multiple-resistance was observed with pyriproxyfen, spirotetramat, or acetamiprid in either group. These findings provide valuable insights for future monitoring and developing insecticide resistance management strategies for B. tabaci.

Factors affecting grain loss reduction efforts among Chinese farmersOriginal Paper

Pan Liu, Jiamin Zhu, Wenwen Pan, Bingjun Li, Qian Liu

Agric. Econ. - Czech, 2025, 71(8):423-432 | DOI: 10.17221/305/2024-AGRICECON

In this study, we used psychology and organisational behaviour theories to determine the main elements affecting Chinese farmers' grain loss-reduction efforts (LREs). We established social factors (SFs), policy factors (PFs) and buyer's strategies (BSs) as the three central factors and constructed a theoretical framework linking these factors to grain producers' LREs. Using this framework, we designed a survey questionnaire and an interview guide; we subsequently collected 1 536 valid questionnaire responses and 25 interview transcripts. The findings revealed that SFs, PFs and BSs had a significance positive effect on the LREs of grain producers. Critically, grain producers from significant Chinese grain-producing regions did not always minimise the effects of the independent variables (SF, PF and BS) on the dependent variable (LRE). This research provides a new viewpoint on grain farmers' LREs activities and may be used to tailor policy and commercial strategies.

Comprehensive comparative study of Chiari-like malformation in veterinary and human medicineReview

JH Jung, H Park, WS Kim, HY Yoon

Vet Med - Czech, 2024, 69(7):217-233 | DOI: 10.17221/125/2023-VETMED

This review aims to enrich our understanding of Chiari-like malformation (CLM) by combining human and veterinary insights, and providing a detailed cross-species overview. CLM is a developmental abnormality characterised by caudal displacement of the hindbrain into the foramen magnum due to an entire brain parenchymal shift caused by insufficient skull volume. This malformation leads to a progressive obstruction at the craniocervical junction, which disrupts the normal cerebrospinal fluid flow, leading to secondary syringomyelia. The clinical signs of CLM and syringomyelia include phantom scratching, head tilt, head tremor, ataxia, tetraparesis, pain, muscle atrophy, and scoliosis or torticollis. Magnetic resonance imaging remains the gold standard for diagnosing CLM, since it allows the visualisation of abnormal findings such as the caudal cerebellar herniation, caudal cerebellar compression from occipital dysplasia, and attenuated cerebrospinal fluid cisternae. Although various medical and surgical interventions, including foramen magnum decompression, can provide temporary symptomatic/clinical sign relief, current literature shows a lack of sustained long-term efficacy. Therefore, additional research is needed to evaluate the long-term effects of existing treatment strategies and to compare different techniques utilised in conjunction with foramen magnum decompression.

Contribution to the turbidimetric method for sulphur determination in arable soilsOriginal Paper

Olya Surleva, Katrin Chavdarova, Vilyana Kazanlaklieva, Lyudmila Angelova, Andriana Surleva

Plant Soil Environ., 2025, 71(10):695-707 | DOI: 10.17221/250/2025-PSE

Sulphur (S) plays an important role in agriculture, being the fourth major contributor to improved quality of crops and increased yields. The applied methods for the estimation of different forms of S in soil aimed at assessing the sulphur availability to plants in various conditions. Nowadays, the wider spreading of regions with sulphur deficiency imposes optimisation of the soil testing procedures in order to increase their availability for laboratories. This study contributes to improving the analytical performance of the turbidimetric method in determining water-soluble sulphate in soil after leaching with the CaCl2 reagent. The modified testing protocol showed: method limit of quantification of 5.0 mg/kg; precision as relative standard deviation less than 3%; recovery of fortified soil samples 103 ± 18%. The expanded uncertainty was 2.3 mg/kg SO42–-S (K = 2, norm.). The proposed testing protocol was inexpensive, fast, used simple equipment and procedures, easily adoptable in regular laboratories, and showed characteristics suitable for the estimation of water-soluble sulfate in arable soils. A set of 546 soil samples was tested, and 74% were found to be sulphur deficient with SO42–-S < 10 mg/kg and sulfur availability index < 6.0. Thus, the availability of laboratory analysis to a broader group of farmers could contribute to effective fertilisation programs, as the newly proposed fertiliser blending technologies are based on adequate estimation of sulfur availability in arable soils.

Piriformospora indica incumbers the incidence and growth of Colletotrichum capsici in colonized chilli plants by direct antagonism and activation of phenylpropanoid pathwayOriginal Paper

Elizabeth T. Jojy, P. Amrutha, Joy Michal Johnson, A. Sajeena, M. Rafeekher, N. V. Radhakrishnan, Ambily Paul

Plant Protect. Sci., 2024, 60(4):354-370 | DOI: 10.17221/119/2023-PPS

Chilli anthracnose, incited by Colletotrichum capsici, is a major disease affecting the quality and quantity of chilli production. Farmers greatly depend on synthetic fungicides for the management of the disease. However, the extensive and non-judicious use of chemical fungicides resulted in the development of fungicide resistance in the pathogen and associated human and animal health risks. Piriformospora indica, a beneficial fungal root endophyte, has been employed as an efficient and safe biocontrol agent for managing bacterial, fungal and viral diseases and enhancing growth and yield. Hence, the present study was carried out to establish the protective role of P. indica against the chilli anthracnose incitant, C. capsici. The enzymes of phenylpropanoid pathway involved in this tripartite interaction were also studied. The study demonstrates that P. indica restricted C. capsici growth in dual culture with 57.22% mycelial inhibition on the 15th day after inoculation. P. indica ‒ colonized chilli plants showed a delay in disease development, and significantly reduced the incidence and severity of chilli anthracnose disease compared to the control plants. Higher activities of defence-related enzymes viz. peroxidase, phenylalanine ammonia-lyase, polyphenol oxidase, 4-coumaryl CoA ligase, cinnamyl alcohol dehydrogenase and total phenol in the P. indica ‒ colonised plants revealed that the endophyte early resistance of plants against further pathogen invasions. The present study revealed P. indica to be an efficient biocontrol agent against chilli anthracnose. The results showed that P. indica reduced the infection of C. capsici by direct antagonism, activation of enzymes involved in plant defence and enhanced growth in chilli plants.

Perception of resilience and its determinants with a focus on farm size: The case of agricultural enterprises in the Czech RepublicOriginal Paper

Ilona Svobodová, Marie Šimpachová Pechrová, Milan Takáč

Agric. Econ. - Czech, 2025, 71(8):458-474 | DOI: 10.17221/479/2024-AGRICECON

The aim of the paper is to examine the resilience of Czech agricultural enterprises through the perceptions of farm managers and to assess the areas where these businesses perceive themselves as resilient and where they feel vulnerable. The analysis is based on data from a survey conducted by the Institute of Agricultural Economics and Information (IAEI) in June 2022, involving 521 Czech agricultural managers. The study focuses on six key areas: climate change and its impacts, agricultural inputs, labour force, market outlets, land prices and availability, and changes in the Common Agricultural Policy (CAP). Across all areas, managers reported that businesses were more frequently vulnerable than resilient, particularly in relation to agricultural inputs and climate change. The results also highlight differences in the perception of resilience across enterprises of varying sizes, with significant variation noted in how changes to the CAP were viewed. Contrary to the common belief that larger enterprises are more resilient, the survey found that Czech medium-sized and large enterprises (with 200 hectares or more of agricultural land) were more frequently perceived as vulnerable.

Temporal variations in flowering of ‘Honeycrisp’ apple grafted on eight different rootstocksOriginal Paper

Oscar Cruz-Alvarez, José de Jesus Ornelas-Paz, Diana Laura Araujo-Pallares, María Nohemí Frias-Moreno, Graciela Dolores Ávila-Quezada, Damaris Leopoldina Ojeda-Barrios, Juan Luis Jacobo-Cuellar

Hort. Sci. (Prague), 2025, 52(3):211-223 | DOI: 10.17221/32/2024-HORTSCI

Malus domestica is one of the world’s most important deciduous fruit trees. Over a four-year period (2017–2020), temporal variations in flowering were evaluated in ‘Honeycrisp’ apple trees on eight rootstocks (G.30, G.969, G.202, G.41, G.11, M.9T337, M.26 EMLA and V.6) planted in 2014 in Chihuahua, México. Among the variables evaluated were the probability of late-spring frost, winter chill units, growing degree days, flowering period, foliar nutrient concentrations, trunk cross-sectional area, number and weight of fruit per tree, and production efficiency. Significantly different chill unit accumulations occurred over the four years, with values falling between 974 and 1 415, where for the latter value, the start of flower opening was earlier, but there was a higher risk of damage by temperatures ≤ –2 °C. There was no effect of rootstock on the time of onset and end of flowering. The most productive combinations were ‘Honeycrisp’ on G.969, G.11 and V.6 with yield estimates of 35 300, 34 200 and 33 600 kg/ha, respectively. The commercial production of ‘Honeycrisp’ apple trees requires the evaluation of their agronomic performance with different rootstocks. Flowering is particularly important since this phenological stage is so closely linked to productivity and is strongly affected by variations in winter temperatures.

Study on the drying kinetics of Rosa flower buds using different drying methodsOriginal Paper

Hamid Reza Gazor, Omid Reza Roustapour

Res. Agr. Eng., 2025, 71(4):213-223 | DOI: 10.17221/43/2025-RAE


This study investigated the drying kinetics of Rosa flower buds (Rosa damascene Mill) under environmental conditions (shade), as well as in direct and indirect solar dryers. The effects of 40 °C, 50 °C, and 60 °C temperatures on the drying of the buds were also examined using a laboratory cabinet dryer. The drying rate of the Rosa flower buds was compared with various mathematical models, and the parameters of these models were evaluated. The results illustrated that drying Rosa flower buds under shade required a lengthy period time of approximately 13 days. In contrast, utilising solar dryers significantly reduced the drying period time for Rosa flower buds. Also, compared to the ambient drying method, the use of indirect solar dryers had the most substantial effect on decreasing the drying period time of the buds up to 86.6%. Furthermore, applying a temperature of 60 °C in the laboratory dryer reduced the drying time of the buds by 76.2% compared to a temperature of 40 °C. The Midilli et al. model (MDM), Page model (PM) and approximate diffusion model (ADM) demonstrated a good fit with the experimental data and can be employed to represent the drying behaviour of Rosa flower buds. The effective of moisture diffusivity of Rosa flower buds during drying was found to be in a range from 6.87 × 10–12 to 1.89 × 10–10 m²·s–1 and the activation energy values were determined as 65.30 and 72.80 kJ∙mol–1 for buds which were dried in the laboratory cabinet dryer and those dried using the ambient and solar dryer methods, respectively.

Effect of agricultural socialisation services on green grain production efficiency: Evidence from Jiangsu Province, ChinaOriginal Paper

Yue-Dong Zhang, Jing-Jing Li, Yi-Fang Zheng, Jia-Xian Xu

Agric. Econ. - Czech, 2025, 71(4):173-184 | DOI: 10.17221/296/2023-AGRICECON

In this study, we examine the effect of Agricultural Socialisation Services (ASS) on green grain production efficiency in Jiangsu Province, China, by using data from the China Land Economy Survey. We used the generalised random forests model in this research to address potential issues of farming household self-selection into ASS and unobserved heterogeneity in treatment effects. The results show that participation in ASS significantly improves green production efficiency, particularly for small-scale farmers. Efficiency gains are most pronounced in critical agronomic operations such as pest control, seeding and planting, whereas smaller efficiency effects are observed in plowing, harvesting and straw treatment. The findings suggest that targeted expansion of ASS could substantially enhance sustainable farming practices, especially for resource-constrained farms. This study provides important policy insights for promoting agricultural sustainability through improved access to and delivery of agricultural services, contributing to more efficient and ecofriendly grain production.

Effect of extruded flaxseed supplementation during the indoor fattening of yearling bulls on beef carcass, meat composition, and fatty acid profileOriginal Paper

Emir Mujić, Javier Mateo, Mahsa Dehnavi, Halil Omanović, Aida Džaferović, Munevera Begić, Selma Čorbo

Czech J. Anim. Sci., 2025, 70(12):518-527 | DOI: 10.17221/5/2025-CJAS


This study investigates the impact of supplementing extruded flaxseed to the diet of yearling bulls during the indoor fattening on beef carcass quality, meat composition, and fatty acid profile. Twenty male crossbred calves (Holstein × Simmental) were divided into two groups: control group and flaxseed-supplemented group, each with 10 calves. The control group received a conventional diet, while the flaxseed group was fed the same diet enriched with 5% extruded flaxseed on a dry-matter basis. The study revealed no significant differences in growth performance, carcass weight, or yield between the two groups. However, the flaxseed-supplemented group exhibited a higher intramuscular fat content, which was likely due to the increased energy intake from the flaxseed lipid content. Additionally, flaxseed supplementation improved the fatty acid profile of beef by increasing the percentage of monounsaturated fatty acids (MUFA) and reducing the saturated fatty acid (SFA) percentage and the n-6/n-3 polyunsaturated fatty acid ratio. Despite these improvements, the study did not reveal any significant increase in the proportion of n-3 fatty acids in the meat. These findings suggest that while flaxseed supplementation enhances the nutritional profile of beef, further research is needed to optimise the balance of energy intake to maximise the increase in n-3 fatty acids.

Do stand structure and admixture of tree species affect Scots pine aboveground biomass production and stability on its natural site?Original Paper

Ondřej ŠPULÁK, Jiří SOUČEK, Jakub ČERNÝ

J. For. Sci., 2018, 64(11):486-495 | DOI: 10.17221/117/2018-JFS

The paper analyses stand structure and production on two experimental forest stand series of mature pure and mixed Scots pine stands, growing on natural Scots pine sites in the Czech Republic. Sessile oak was the main admixed species. In mixed stands, Scots pine constituted the dominant level of the stand, admixed species grew mostly as subdominants. Admixture increased stand densities and aboveground biomass production compared to pure stands. Sessile oak with the 20-30% number share within the Scots pine stand led to an increase of the Scots pine tree dimensions and mean stem merchantable wood (DBH ≥ 7 cm) volume compared to the pure Scots pine stand of similar density. The Scots pine and sessile oak slenderness ratios increased in mixed stands compared to monocultures, however, the stand mechanical stability was not threatened.

Molecular identification of wheat leaf rust resistance genes in sixty Chinese wheat cultivarsOriginal Paper

Zhikuan REN, Zaifeng LI, Lingzhi SHI, Xiaodong WANG, Lin ZHU, Xing LI, Daqun LIU

Czech J. Genet. Plant Breed., 2018, 54(1):1-8 | DOI: 10.17221/6/2016-CJGPB

Common wheat (Triticum aestivum L.) is the major crop cultivated in Xinjiang and Anhui provinces of China. The climate in these two provinces is favourable for wheat leaf rust (Puccinia triticina) (Pt) infection. Here, we demonstrate a detailed investigation on the leaf rust resistance of 60 major wheat cultivars cultivated in these two regions. A mixture of high virulent Pt races (THTT, THTS, THTQ and PHPS) were used to phenotype all the collected wheat cultivars at an adult plant stage. Phenotypic disease severity (FDS) and the area under the disease progress curve (AUDPC) for each of these wheat cultivars were calculated. Among all the tested wheat cultivars, three cultivars (Xindong20, Xindong 29 and 99AR142-1) with the lowest FDS and AUDPC may carry major resistance genes. Twenty-seven cultivars (45% of the total tested ones) showed a relatively lower resistance with an average of 12.52% FDS and 126.3 AUDPC. Minor resistance or slow rusting genes may be present in this group of cultivars. Molecular markers for leaf rust resistance genes Lr1, Lr9, Lr19, Lr24, Lr26 and Lr34 were further used for the genotypic screening. Lr1, Lr19, Lr26 and Lr34 were detected in 19 (31.7%), 1 (1.7%), 12 (20%) and 6 (10%) wheat cultivars, respectively. Neither Lr9 nor Lr24 could be detected in any of the tested cultivars. These results will greatly improve wheat molecular breeding for leaf rust resistance in these areas.

Determination of carcinoembryonic antigen and cancer antigen values with the radioimmunoassay method in healthy females dogsOriginal Paper

V. Ledecky, A. Valencakova-Agyagosova, J. Lepej, Z. Frischova, S. Hornak, V. Nagy

Vet Med - Czech, 2013, 58(5):277-283 | DOI: 10.17221/6810-VETMED

The aim of this study was to determine reference values of carcinoembryonic antigen and cancer antigen in 32 clinically healthy bitches. The average age of the bitches in each group was as follows: small breeds 3.50 ± 2.30, medium breeds 3.83 ± 3.21, large breeds 6.00 ± 3.22 and giant breeds 2.40 ± 2.43. The average weight in each group was as follows: 1st group 7.94 kg ± 1.84, 2nd group 22.38 kg ± 2.77, 3rd group 35.94 kg ± 7.16, and 4th group 52.75 kg ± 5.04. The cancer markers were determined using human kits. The mean values of the carcinoembryotic antigen markers ± SD were as follows: 1st group 0.18 ± 0.03, 2nd group 0.20 ± 0.03, 3rd group 0.22 ± 0.01, 4th group 0.18 ± 0.04. The statistical significance for the carcinoembryonic antigen markers was P = 0.0042**. The values of cancer antigen markers ± SD were: 4.90 ± 1.04, 4.80 ± 1.13, 5.90 ± 1.22, and 4.72 ± 0.97, respectively. The cancer antigen values were statistically insignificant (P = 0.1762). Based on obtained values of the mean 95%, we expect a standard for carcinoembryonic antigen of 0.00-0.23 ng/ml and for cancer antigen 0.0-7.00 IU/ml. The results of the present study show that it is possible to use human kits for the determination of carcinoembryonic antigen and cancer antigen in clinically healthy bitches using the radioimmunoassay method.

Estimation of genetic parameters of fatty acids composition in flesh of market size common carp (Cyprinus carpio L.) and their relation to performance traits revealed that selective breeding can indirectly affect flesh qualityOriginal Paper

Martin Prchal, Marc Vandeputte, David Gela, Marek Doležal, Hana Buchtová, Marek Rodina, Martin Flajšhans, Martin Kocour

Czech J. Anim. Sci., 2018, 63(7):280-291 | DOI: 10.17221/30/2018-CJAS

Fish are a rich source of omega-3 polyunsaturated fatty acids (n-3 PUFAs) and thus, they should be an integral part of human diet at least twice a week. As a result, high attention has been devoted to the improvement of fatty acids (FA) content in the flesh of farmed fish through nutrition. Conversely, there are very few data on the potential of selective breeding to improve FA composition in fish. We estimated genetic parameters of fillet fatty acid content and performance traits in market size common carp cultured under semi-intensive pond conditions. The experimental stock arose through factorial mating of 7 dams and 36 sires. All families were reared communally. Pedigree was reconstructed with microsatellite markers, and 158 individuals were dressed out and selected for flesh FA composition analysis. Heritability estimates of total muscle fat, FA composition in total fat (TF) (n-3 PUFA-TF, PUFA-TF, EPA-TF - eicosapentaenoic acid, n-6/n-3 - omega6/omega3 PUFA ratio), and most performance traits were moderately heritable (h2 = 0.23-0.41), and body weight was highly heritable (h2 = 0.62 ± 0.20). Genetic correlations show that selection for faster growth would indirectly lead to fillet yield improvement (rg = 0.50-0.62) while having little impact on muscle fat (rg = 0.21). However, lipid quality in flesh would be affected: n-3 PUFA-TF would decrease and the n-6/n-3 PUFA ratio would increase. A likely interpretation is that faster growing genotypes consume more supplemental feed, which was poor in the beneficial FAs. For sustainable selective breeding, supplemental feed composition should be modified, so that faster growing carps would maintain an appropriate flesh quality.

The use of Snyder synthetic hydrograph for simulation of overland flow in small ungauged and gauged catchmentsOriginal Paper

Darya FEDOROVA, Pavel KOVÁŘ, Jan GREGAR, Andrea JELÍNKOVÁ, Jana NOVOTNÁ

Soil & Water Res., 2018, 13(4):185-192 | DOI: 10.17221/237/2017-SWR

The paper presents the results of simulated overland flow on the Třebsín experimental area, Czech Republic, using the Snyder synthetic unit hydrograph. In this research an attempt was made to discover a new approach to overland flow simulation that could give precise results like the KINFIL model for a small ungauged catchment. The provided results also include a comparison with the KINFIL model for N = 10, 20, 50 and 100 year recurrence of rainfall-runoff, with the rainfall time duration td = 10, 20, 30, and 60 min. Concerning a small gauged catchment, one of the most accurate and elegant methodologies, Matrix Inversion Model, can be used for the measurement of both the gross rainfall and the runoff. This method belongs to a matrix algebra concept. For the sake of completeness, we designated this model at the end of the present article to show how exact this forward march can be.

Yield and crown structure characteristics in a black locust (Robinia pseudoacacia L.) stand: A case study - Short CommunicationShort Communication

Károly RÉDEI, Beatrix BAKTI, Tamás KISS, Marianna TAKÁCS, Zsolt KESERŰ

J. For. Sci., 2018, 64(2):96-100 | DOI: 10.17221/118/2017-JFS

The paper provides the results of a detailed analysis of timber volume and the most important crown variables of black locust (Robinia pseudoacacia Linnaeus) based on an experimental plot in southern Hungary. At the age of 20 years the crop trees belonged to different height classes. If the volume of the mean tree from height class I is considered as 100%, the volume of the mean tree of class II is 44.0%, and the mean tree of class III is only 30.3%. In case of timber volume per 1 m2 crown surface, the values are 72.9 and 61.7%. The DBH of trees showed a positive linear correlation with crown diameter (r = 0.942). Additionally, there were also positive linear relationships between crown diameter and volume (r = 0.901), between crown length and volume (r = 0.721) as well as tree height and crown length (r = 0.661). The variation of crown indices is height even within the same stand and indicates the importance of following a selective thinning operation method.

Analysis of therapeutic results and complications after colic surgery in 434 horses

J. Mezerova, Z. Zert, R. Kabes, L. Ottova

Vet Med - Czech, 2008, 53(1):12-28 | DOI: 10.17221/1934-VETMED

Out of the total number of 434 horses that underwent colic surgery, small intestine was operated in 195 (44.9%) patients, caecum in 10 (2.3%) horses, large colon surgery was performed in 196 (45.2%) cases and small colon surgery in 14 (3.2%) horses. In 12 patients (2.8%) two different parts of the gastrointestinal tract were affected simultaneously, one horse suffered from peritonitis, torsion of the uterus developed in two mares and three animals had negative surgical findings. Of 434 horses, 371 (85.5%) survived. After small intestinal surgery, 159 patients (81.5%) recovered from anaesthesia and were discharged home as well as seven horses (70%) after caecal surgery, 175 horses (89.3%) after large colon surgery and 14 horses (100%) following small colon surgery. 75 out of 103 horses (72.8%) were discharged home after the small intestinal resection and 89 of 98 horses (90.8%) with small intestinal problems where no resection was needed. In total, 43 of the patients that underwent one surgery did not survive the immediate postoperative period. The most frequent lethal complications in horses following the small intestinal surgery included peritonitis (five horses) and paralytic ileus (four horses) and in horses with large colon problems it was typhlocolitis (six cases). Relaparotomy was indicated in 41 of 434 horses (9.4%) that recovered from colic surgery. 21 out of the 41 (51.2%) relaparotomised colic patients were released from the clinic. All successfully repeated surgeries were carried out to overcome primary small intestine ileus problems, and in 14 of these cases (66.7%) resection and anastomosis were performed. The most common finding, diagnosed in 9 of 21 reoperated horses, was paralytic ileus. Of 20 relaparotomised horses that did not survive, three animals were lost after the introduction of anaesthesia, nine horses were euthanised after the abdominal cavity revision, one horse did not recover after the surgical procedure and seven horses did not survive the postoperative period. In 15 of 20 dead horses, the cause of the first surgical intervention was small intestinal ileus, in other four horses there was a large colon problem and in the last patient, it was a stomach disease. In 13 of 15 (86.7%) horses with small intestinal problems and in three of four (75%) patients with large colon disease, either resection or bypass was performed. In the remaining four non-surviving horses of 20 relaparotomised ones, peritonitis and/or adhesion formation was diagnosed at the second surgery, in three horses anastomosis complications were the main problem. Peritonitis or paralytic ileus led to death or euthanasia in four of seven horses that recovered after relaparotomy.

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cowsOriginal Paper

A. Vernunft, J.M. Weitzel, T. Viergutz

Vet Med - Czech, 2013, 58(4):221-229 | DOI: 10.17221/6760-VETMED

Secretion of adequate levels of progesterone from a proper corpus luteum (CL), which develops out of the cells of a healthy preovulatory follicle, is a key-factor for establishment of a pregnancy. The aim of this study was to investigate the relationship between morphological and secretory characteristics of preovulatory follicles and their corresponding corpus luteum with regard to the post-partum period in high-yielding dairy cows. Therefore, ultrasound-guided aspirations of preovulatory follicles were performed repeatedly, using 20 first lactating cows between 26 and 121 days after parturition. Heat was induced with a PGF analogon followed by administration of a GnRH analogon. The dominant follicle was aspirated 21 h after administration of the GnRH analogon. The diameters of the follicles were estimated at aspiration and the morphology of the resulting luteal tissue was examined on day 14 after follicle aspiration using ultrasonographic examinations. Concentrations of progesterone (P4) and 17-beta-oestradiol (E2) were determined in the follicular fluids (FF) and P4 concentration was estimated at the time of CL examination in plasma. A CL development occurred in 82% after dominant follicle aspiration. The interval of time between parturition and follicle aspiration did not affect the investigated variables. The diameter of the aspirated preovulatory follicle was positively correlated to the cross-section area of the developed luteal tissue (R = 0.60; P < 0.01) as well as to the plasma P4 concentration on day 14 after follicle aspiration (R = 0.47; P < 0.05). Also, E2 concentrations in FF were positively correlated to cross-section area of the luteal tissue (R = 0.54; P < 0.05). Comparing the FF of the follicles that gave rise to a CL after aspiration to follicles that did not, both types had comparable P4, but the former type harboured higher E2 concentrations. In conclusion, preovulatory follicle diameter as well as steroid concentrations in the follicular fluid could be used prospectively to identify cows which will have well-developed CLs and high plasma P4 levels later. On the other hand, CL development after follicle aspiration can be used as a retrospective quality parameter of dominant follicles. These results will help to identify suitable animals for breeding or recipients for embryo transfer.

The effect of seed priming on field emergence and root yield of sugar beetOriginal Paper

Beata MICHALSKA-KLIMCZAK, Zdzisław WYSZYŃSKI, Vladimír PAČUTA, Marek RAŠOVSKÝ, Agnieszka RÓŻAŃSKA

Plant Soil Environ., 2018, 64(5):227-232 | DOI: 10.17221/136/2018-PSE

The effect of sugar beet seed (primed and non-primed) on field emergence and root yield of sugar beet was examined. The experiment was realized in the years 2012-2014 at an Experiment Field Station of Warsaw University of Life Sciences - SGGW Faculty of Agriculture and Biology in Skierniewice (51°97'N, 20°19'E) in Poland. The experimental factor was diversified seed material of the same cultivar of sugar beet - typical seeds, traditionally prepared for sowing (non-primed seeds) and seeds before sowing, subjected to the process of priming. On average for the three years of the study, no significant effect of seed priming on the field emergence was found. On the other hand, the sugar beet emergence on plots with primed seeds was faster, more even and uniform. Seed priming, on average for the three years of the study, significantly increased the mean root mass during harvest. In contrast, priming the seeds did not cause an increase in the final plant density. No significant effect of seed priming on root yield was found, both on average for the studied period and in particular years of the study.

Mitigation of ammonia volatilization with application of urease and nitrification inhibitors from summer maize at the Loess PlateauOriginal Paper

Muneer AHMED, 3, Weijia YU, Ming LEI, Sajjad RAZA, Jianbin ZHOU

Plant Soil Environ., 2018, 64(4):164-172 | DOI: 10.17221/46/2018-PSE

Field experiments were conducted at three sites: Yangling (YL); Zhouzhi-1 (ZH-1) and Zhouzhi-2 (ZH-2) of the Loess Plateau during summer maize crop, to investigate the effectiveness of N-(n-butyl) thiophosphoric triamide (NBPT) and NBPT + dicyandiamide (DCD) with urea on reducing NH3 volatilization from different soils under different environmental conditions. Four treatments including control (no N), N-220 kg/ha, N-220 + NBPT and N-220 + NBPT + DCD were applied in two splits through the band placement method. Total NH3-N loss observed were 65.8, 40.5 and 20.1 NH3-N kg/ha (accounting for 29.9, 18.4 and 9.2% of N applied) from urea for YL, ZH-1 and ZH-2, respectively. The application of NBPT and NBPT + DCD significantly reduced NH3 volatilization by 80-93% and 75-90%, respectively. The meteorologic factors such as precipitation, air temperature and wind speed significantly affected NH3 volatilization. These results suggested that the amendment of urea with NBPT and NBPT + DCD have potential to mitigate NH3-N losses from alkaline soils in the Loess Plateau.

Functional properties of blade tiller working toolsOriginal Paper

Radek Pražan, Josef Hůla, Pavel Kovaříček, Jakub Čedík, Ilona Gerndtová, Marcela Vlášková

Res. Agr. Eng., 2018, 64(2):63-69 | DOI: 10.17221/106/2017-RAE

After the harvest of winter wheat, the tensile force, cross section of loosened soil and specific resistance of soil were measured on a loamy soil plot. During the comparative measurements, 3 blades were used with chisel width of 20 mm, 40 mm and 75 mm. In case of all blades, the measurements were carried out at the depth of 100, 200 and 300 mm. In the course of measurements, the standards of blades were attached in a measuring frame on six-component hinge with connected tensometric sensor. The selected blades were developed for a new type of tiller destined for primary cultivation of soil. Moreover, in case of a blade equipped by chisel there were used side wings of a width of 110, 150 and 200 mm for tillage into a depth of 100 mm. It was found that the width of blade chisels did not have a statistically significant effect on the values of specific resistance of soil during the soil tillage. At all widths of blade chisels, a trend of an increase in specific resistance of soil was recorded at growing recess into the soil.

Isolation and characterization of polymorphic microsatellite markers in Toxicodendron vernicifluumOriginal Paper

Dinh-Duy VU, Thi Tuyet-Xuan BUI, Thi Hong-Nhung NGUYEN, Syed Noor Muhammad SHAH, Ngoc-Ha VU, Ya-Hong ZHU, Lei ZHANG, Yi ZHANG, Xiao-Hua HUANG

Czech J. Genet. Plant Breed., 2018, 54(1):17-25 | DOI: 10.17221/183/2016-CJGPB

A total 20 074 230 sequencing reads were generated by Illumina HiSeq2500 from three different Toxicodendron vernicifluum tissue samples. In total, 48 693 unigenes with an average length of 703.34 bp were obtained by de novo assembly. 3392 potential EST-SSRs (expressed sequence tag-simple sequence repeat) were identified as potential molecular markers from unigenes with lengths exceeding 1 kb. A total of 80 pairs of PCR primers were randomly selected to validate the assembly quality and develop EST-SSR markers from genomic DNA. Of these primer pairs, 14 primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism within the lacquer tree population in Langao, Shaanxi province, China. There were high genetic diversities (number of alleles per locus (A) = 2.93, polymorphic information content (PIC) = 0.53, observed heterozygosity (Ho) = 0.62 and expected heterozygosity (He) = 0.85) in the lacquer tree natural population. The four loci were significantly deviated from Hardy-Weinberg equilibrium. These results suggested high homozygosity in the population and low or deficiency in heterozygosity (inbreeding coefficient (Fis) = 0.27). These polymorphic EST-SSR markers will provide the base for further studies of genetic structure and breeding in T. vernicifluum.

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