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Results 1351 to 1380 of 5824:

Insecticidal activity of essential oil from Jasminum sambac (L.) Aiton against Callosobruchus maculatus (Fabricius, 1775) (Coleoptera: Bruchidae)Original Paper

Ruchuon Wanna, Phirayot Khaengkhan, Darika Bunphan, Benjapon Kunlanit, Parinda Khaengkhan, Hakan Bozdoğan

Plant Protect. Sci., 2023, 59(4):369-378 | DOI: 10.17221/69/2023-PPS

Mungbean seeds [Vigna radiata (L.)] are easily damaged by insects during storage, and essential oils (EOs) have proved effective for controlling insect infestation. This research study assessed the bioactivity of EO from Jasminum sambac (L.) Aiton against Callosobruchus maculatus (F.). The chemical composition of EO was analysed by a gas chromatograph-mass spectrometer (GC-MS). All experiments were conducted following a completely randomised design (CRD) with four replications of the residual contact and seed dressing bioassays. Results indicated that J. sambac EO contained 35 compounds (97.587%), with the main component 3-hexen-1-ol benzoate. Residual contact toxicity LC50 values of this EO on adult C. maculatus at 24, 48, and 72 h were 5.01, 4.00, and 3.13 µL/mL, respectively. J. sambac EO at 8 µL/L showed the highest residual contact efficacy against C. maculatus, killing up to 100% in 72 hours. The highest concentration of J. sambac EO (1 mL/kg) gave optimal protection against C. maculatus within 4 and 5 days with 100% mortality. This concentration showed 100% inhibition of adult F1 progeny emergence, with no seed damage and weight loss of V. radiata L. after 30 days of exposure, and not significantly different from 0.8 mL/kg. Results indicated that J. sambac EO effectively prevented C. maculatus infestation and it could be used as an alternative to commercial chemical insecticides.

Understanding the role of ecotypic factors in the early growth of Pinus sylvestris L.Original Paper

Jakub Hejtmánek, Jan Stejskal, Daniel Provazník, Jaroslav Èepl

J. For. Sci., 2023, 69(12):539-549 | DOI: 10.17221/102/2023-JFS

The ecological significance of Scots pine (Pinus sylvestris L.) in Europe, especially in areas devastated by bark beetles, has led to its consideration as a substitute for Norway spruce. This pioneering species boasts sun and drought tolerance, fast growth, and wood industry value. To gauge its potential, we examined two ecotypes across two test sites over two years, focusing on height and growth increment. Through statistical analysis employing R software and linear mixed models, we assessed heritability, genotype by environment interaction, and spatial correlations. Both ecotypes exhibited significant differences in height and increment, varying by year and site. Heritability was higher in the second year, with increment showing greater stability. Genetic correlations between sites were evident, suggesting stable increment ranking across locations. These findings underscore the role of ecotypic variation in Scots pine growth, advocating for its consideration in reforestation. Acknowledging such dynamics is vital for effective forest management and reforestation in Central Europe, promoting sustainability and informed decision-making. Further research will enhance this understanding of preserving and enhancing the region's tree populations.

Label-free proteomics to identify keratins and keratin-associated proteins and their effects on the fleece traits of Inner Mongolia Cashmere GoatsOriginal Paper

Chongyan Zhang, Yuchun Xie, Juntao Guo, Xin Su, Cun Zhao, Qing Qin, Dongliang Dai, Zhixin Wang, Jinquan Li, Zhihong Liu

Czech J. Anim. Sci., 2023, 68(1):17-29 | DOI: 10.17221/93/2022-CJAS


Inner Mongolia Cashmere Goat fleece is derived from a heterogeneous coat. The guard hair is produced by primary hair follicles, and the down hair is produced by secondary hair follicles. Reports have focused on research related to the different hair follicle types, but no related study has addressed the difference in the proteins of down hair (DH) and guard hair (GH), and whether the protein composition of these materials differs remains to be studied. The protein composition of DH and GH was studied. A total of 108 proteins were identified in DH, and 116 proteins were identified in GH; 39 proteins were differentially expressed, of which 18 proteins were upregulated and 21 proteins were downregulated in DH versus GH. In this study, through the measurement of DH and GH samples from Inner Mongolia Cashmere Goats, the diameter of DH relative to GH was downregulated (< 0.05), a property that may be related to the KRT38 protein. Moreover, the strength of GH was significantly higher than that of DH (< 0.05), a property that may be affected by the KRTAP8-1 protein. GO analysis showed that the different traits of DH and GH from Inner Mongolia Cashmere Goats are affected by keratin, actin, and calcium-binding proteins. This study uses a nonlabelled quantitative proteomics method to study the proteins in DH and GH, aiming to identify the keratin family in Inner Mongolia Cashmere Goats and to provide a new direction for studying DH and GH traits at the protein level.

The impact of geopolitical risk on agricultural commodity pricesOriginal Paper

Kristína Hudecová, Miroslava Rajèániová

Agric. Econ. - Czech, 2023, 69(4):129-139 | DOI: 10.17221/374/2022-AGRICECON


The escalation of the conflict between Russia and Ukraine had a detrimental effect on the global agricultural and food market and the price movements of essential commodities. In this study, we aim to investigate the effects of geopolitical risk on the prices of selected agricultural and food commodities using the linear and nonlinear ARDL (autoregressive distributed lag) model. Our results show evidence of the asymmetric impact of geopolitical risk on the prices of rapeseed, sugar, sunflower oil, and wheat. The findings also show no long-term link between geopolitical risk and corn, cotton, lumber, milk, oats, rough rice, and soybean prices.

Comparison of gene expression changes in two wheat varieties with different phenotype to strip rust using RNA-Seq analysisOriginal Paper

Congying Yuan*, Yadi Miao, Huihan Zhang, Shiying Liu, Yaoyao Wang

Plant Protect. Sci., 2023, 59(2):134-144 | DOI: 10.17221/125/2022-PPS

The fungus Puccinia striiformis f. sp. tritici (Pst) is an important threat to wheat production because it can cause wheat stripe rust. The present study aimed to identify new stripe rust resistance genes and to provide a theoretical and practical basis for breeding wheat varieties with broad spectrum, stable, and durable resistance. Wheat leaves inoculated with wheat stripe rust fungus Chinese yellow rust 34 were collected at different time points for transcriptomic analysis based on the wheat stripe rust susceptible varieties AVOCET S (AVS) and AVSYr15NIL [near-isogenic line (NIL) derived from AVS]. The results showed that the number of upregulated genes in the two varieties was 294, 364, 398, and 604, and the number of downregulated genes was 520, 178, 570, and 345 on the 1st, 3rd, 5th, and 7th days post inoculation, respectively. Gene Ontology and Kyoto Encyclopedia of Gene and Genomes enrichment analyses found enrichment of differentially expressed genes in the peroxisome proliferators-activated receptor signaling pathways, plant–pathogen interaction, and styrene acrylic acid biosynthesis that encoded protein kinases, signal transduction, transcription factors, and functional protein components. Differentially expressed genes were randomly selected for quantitative reverse transcription PCR analysis, and the change trend was the same as in the transcriptome data. The results of this study suggest that genes in AVSYr15NIL related to the stripe rust response could be valuable for understanding the mechanisms involved in stripe rust resistance.

Effects of phytogenic feed additives on the growth, blood biochemistry, and caecal microorganisms of White Roman geeseOriginal Paper

Shih-Yi Shen, Yuan-Yu Lin, Shih-Chieh Liao, Jhin-Syuan Wang, Sheng-Der Wang, Ching-Yi Lien

Czech J. Anim. Sci., 2023, 68(5):202-211 | DOI: 10.17221/205/2022-CJAS


This study investigated the effects of Lycium chinense Miller stem (LCM) and Origanum vulgare Linn (oregano) essential oil on growth, blood biochemistry, and caecal microorganisms in White Roman goose. A total of 96-day-old White Roman geese were randomly allocated to a control group and three treatment groups. The G 0.05 diet was enriched with 0.05% LCM extract, the G 0.5 diet was enriched with 0.5% LCM extract, and the O 0.05 diet was enriched with 0.05% oregano essential oil. Each treatment was allotted to three pens, with four males and four females per pen. Geese were fed for 83 days. Geese in group O 0.05 had greater body weight gain than those in groups G 0.05 and G 0.5 in the first 28 days (P < 0.05), whereas the geese in group O 0.05 also showed greater body weight gain than those in control and G 0.05 groups in total 83 days (P < 0.05). No significant differences in serum creatinine, glutamic oxaloacetic transaminase, glutamic-pyruvic transaminase, cholesterol, low-density lipoprotein, or high-density lipoprotein were observed. Serum glutathione peroxidase concentrations were significantly higher for geese in group G 0.5 (< 0.05) than for the control group at 83 days of age. At 87days of age, one male and one female per pen were slaughtered, and the caecum contents were collected for gene sequencing by 16S ribosomes. No significant differences in microbial alpha diversity were observed. In conclusion, supplementation with 0.05% oregano essential oil increased body weight gain for 83 days. In addition, supplementation with 0.5% LCM extract increased glutathione peroxidase activity at 83 days of age (< 0.05). In summary, our study showed that phytogenic feed additives improve growth and have antioxidant and immune properties in geese.

Timed artificial insemination protocols in dairy cattle: Functioning, shortcomings, and improvementsReview

Amine Boudaoud

Czech J. Anim. Sci., 2023, 68(6):231-244 | DOI: 10.17221/208/2022-CJAS


Oestrus detection is currently at the top of the factors limiting the success of artificial insemination in dairy herds. This limitation becomes more pronounced as milk production increases because of higher metabolic clearance rates of steroid hormones leading to low circulating oestradiol concentration near oestrus. The standard OvSynch has overcome this problem by enabling fixed-time artificial insemination, for it synchronises follicular wave emergence, luteolysis, and ovulation. However, it did not improve fertility compared to cycles with spontaneous ovulation, which motivated the development of “fertility programs”. These later are nothing but improved OvSynch protocol. This article explains what went wrong with the original OvSynch protocol, discusses the improvements made by “fertility programs”, and highlights the advantages and limits of the newly tested molecules.

Microalgae in lab-grown meat productionReview

Arturo Nickolay Rojas-Tavara, Alberto Jesus Donayre-Torres

Czech J. Food Sci., 2023, 41(6):406-418 | DOI: 10.17221/69/2023-CJFS


Reports have shown that meat production operations today contribute to the climate crisis, facilitating the occurrence of infectious diseases, and contributing to environmental pollution. Consequently, the public demands alternatives to traditional meat, such as in vitro manufactured meat. Several authors have suggested that improvements should be made in the manufacturing of cell-cultured meat to make a more sustainable and scalable process. They recently proposed using microalgae as a sustainable system to produce important nutrients such as oxygen from cellular waste molecules of animal cultures such as ammonia and carbon dioxide. In this review, we discuss recent advances of different microalgae applications in the production of lab-grown meat, with special emphasis on their use as a replacement for fetal bovine serum (FBS) or culture media, as well as its applicability as a source of cell oxygenation and waste upcycling to extend the life of animal cell cultures. Also, we discuss the implementation and limitations of these algae systems in large-scale in vitro meat manufacturing.

The impact of the integration of science, technology, and finance on innovation in listed agricultural enterprises through the lens of digital transformationOriginal Paper

Fanye Kong, Huini Zhong, Nana Chai, Baofeng Shi

Agric. Econ. - Czech, 2026, 72(2):84-100 | DOI: 10.17221/22/2025-AGRICECON

Digitisation enables the integration of science, technology and finance, and this trend toward integration can in turn enhance the innovation efficiency of listed agricultural enterprises. By leveraging the quasi-natural experiment of China's 2011 pilot program for technology finance – also known as science, technology, and finance, this paper uses the difference-in-differences (DID) model to demonstrate that the science and technology finance policy positively impacts the innovation levels of agriculture-related listed enterprises. That said, the policy's effect varies across different regions and enterprise sizes. Furthermore, moderating-effect analysis reveals that the digitalisation level of agriculture-related listed enterprises is a critical factor, while the evolutionary game model elucidates this factor's underlying mechanism. Based on these findings, policy recommendations are proposed to further develop science and technology finance platforms for agricultural-related listed enterprises.

Forest cover change detection using Normalized Difference Vegetation Index in the Oued Bouhamdane watershed, Algeria – A case studyOriginal Paper

Boubaker Khallef, Rabah Zennir

J. For. Sci., 2023, 69(6):254-265 | DOI: 10.17221/192/2022-JFS

The Algeria forest, particularly in the northeastern region, has undergone profound changes in recent years. The Oued Bouhamdane watershed has a great forest potential, which is threatened by several factors of natural and human origin, resulting in a decrease in forest cover. It requires adequate forest monitoring to support the sustainable forest management of this watershed, which is possible thanks to satellite imagery. The objective of this research is to study the spatiotemporal dynamics of the vegetation cover of the Oued Bouhamdane watershed between 2013 and 2022 using remote sensing data. This study is based on the use of Landsat 8 and 9 images for two dates in 2013 and 2022, and the calculation of the Normalized Difference Vegetation Index (NDVI) to identify changes in vegetation cover between 2013 and 2022. The calculation of NDVI and the realization of the vegetation change map showed a regression of the forest cover between 2013 and 2022 with a rate of –5.53% of the total of the study area with a general negative change of 28.62% of the study area. This regression is essentially linked to natural and anthropogenic factors. This work can be a valuable tool for sustainable management of the forest of this watershed; moreover, the method is also adaptable to other watersheds of the northeastern region of Algeria.

Effect of elicitors and biostimulants on the content of bioactive compounds in raspberry fruitsOriginal Paper

Nayelli Azucena Sigala Aguilar, Jose Antonio Gonzales Fuentes, Luis Alonso Valdez Aguilar, Mercedes Guadalupe López Pérez, Julia Medrano Macias Medrano Macias, Adalberto Benavides-Mendoza Benavides Mendoza, Susana González Morales

Hort. Sci. (Prague), 2023, 50(2):101-111 | DOI: 10.17221/98/2021-HORTSCI

Raspberry has acquired great interest in human health due to its content of bioactive compounds that provide protection against diseases caused by non-communicable diseases. Bioactive compounds are mainly represented by secondary metabolites such as phenols, anthocyanins, and flavonoids. Biostimulants and elicitors are substances or microorganisms that provide protection and defence to the physiological processes of plants. The present study evaluated the effect of two elicitors (hydrogen peroxide, salicylic acid) and three biostimulants (humic and fulvic acids, glutamic acid, seaweed extracts) on the content of bioactive compounds in raspberry fruits, agronomic and fruit yield parameters in plants. Hydrogen peroxide increased the content of bioactive compounds such as flavonoids, anthocyanins, omega 3 and oleic acid. Salicylic acid increased the content of flavonoids, anthocyanins, and citric acid in raspberry fruits; the number of fruit loaders and fruits per plant was also increased. Humic and fulvic acids, glutamic acid, and glutamic acid combined with seaweed extracts increased the content of flavonoids and anthocyanins, without affecting growth parameters and fruit yield. Glutamic acid and seaweed extracts were the only treatments that increased the content of palmitic acid, while seaweed extracts increased °Brix content in fruits.

Comparison of the effects of Aloe vera gel and coconut oil on the healing of open wounds in ratsOriginal Paper

O Istek, M Tanrisever, S Kucukler, B Karabulut, A Cevik

Vet Med - Czech, 2023, 68(1):17-26 | DOI: 10.17221/101/2021-VETMED

In this study, the effects of Aloe vera gel and coconut oil on wound healing were investigated and compared in rats. Forty-two Wistar albino rats were used during the experiment, in which they were operated on under general anaesthesia to create two full-thickness open skin wounds (created with a 0.5 cm diameter punch biopsy apparatus) on both back sides of the median line. A total of 42 rats were divided into three groups of 14 animals each to receive the topical application of Aloe vera gel (AV group – n = 14), coconut oil (CO group – n = 14) and cold cream (CONT group – n = 14). The medical applications were performed twice a day in all the groups. The wound borders were marked on a transparent sheet every day. Afterwards, this sheet was transferred to the millimetre graph paper. On days 0, 7, and 14, the unhealed wound area was measured in all the groups. On days 7 and 14, seven rats in each group were euthanised. Then, skin samples including the intact skin were taken from the wound sites for histopathological and biochemical evaluations. The topical application of Aloe vera gel showed a significant increase in the healing process of the open wounds in terms of the clinical evaluation, histopathological and biochemical data averages when compared with the coconut oil and cold cream groups of rats (P < 0.05). The results obtained in the present study demonstrate that Aloe vera gel may provide a good alternative for the treatment of open wounds.

Genetic diversity and pathotype profiling of Xanthomonas oryzae pv. oryzae isolates from diverse rice growing ecosystems of Karnataka state of IndiaOriginal Paper

Adke Raghunandana, Devanna Pramesh, Gururaj Sunkad, Chittaragi Amoghavarsha, Manoj K. Yadav, Umakanta Ngangkham, H.D. Pushpa, M.K. Prasannakumar, B.T. Raghavendra, Harischandra R. Naik, Siddepalli E. Manjunatha, S.T Yenjerappa

Plant Protect. Sci., 2023, 59(1):31-47 | DOI: 10.17221/76/2022-PPS


Bacterial leaf blight (BLB) disease of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the significant constraints for rice production. A study was carried out to analyze the virulence, genetic diversity, and population structure of Xoo isolates collected from different rice ecosystems of Karnataka, India. The taxonomic identity of all 54 isolates was confirmed using a Xoo-specific PCR assay. The virulence assay on a set of twenty-eight near-isogenic differentials identified nine pathotype groups (KPthX-1 to KPthX-9). Among them, KPthX-6 was highly virulent, whereas KPthx-2 was less virulent. Differentials with four and five resistance (R) genes (Xa4+xa5+xa13+Xa21, Xa4+Xa7+xa13+Xa21, and Xa4+xa5+Xa7+xa13+Xa21) were effective against all the pathotypes. Further, genetic diversity was deduced using a universal marker set, JEL-1, and JEL-2, complementary to a conserved repetitive sequence IS1112. The amplicon size and numbers varied from 100 bp to 3 Kb and 1 to 25. Based on the amplicon counts, Jaccard’s co-efficient and phylogenetic analyses were carried out and categorized the 54 isolates into three clusters (I to III). Cluster-II contains the maximum number of isolates (27), followed by cluster-I (23 isolates) and cluster-III (one isolate). The structure analysis categorized the isolates into five subgroups, viz. SG1, SG2, SG3, SG4, and SG5 indicate the existence of five subpopulations. The present study has identified the genetic and virulence diversity of the Xoo population and reported the effective R gene/s for different rice ecosystems, which can be used in the breeding program for the eco-specific management of BLB of rice.

Recent development of economic indicators on Czech dairy farmsOriginal Paper

Jan Syrùèek, Ludìk Bartoò, Dalibor Øehák, Magdaléna ©tolcová, Jiøí Burdych

Agric. Econ. - Czech, 2023, 69(2):45-54 | DOI: 10.17221/381/2022-AGRICECON


A reassessment of factors related to milk production economics is needed because of continuing genetic improvements within the main dairy breeds and changes in farm management and the economic environment. Therefore, in this study, we aimed to evaluate the development of economic indicators between 2016 and 2020 on Czech dairy farms that kept either Czech Fleckvieh or Holstein breeds and that had different average milk yields. We used a questionnaire to obtain data from 66 dairy farms from different regions of the Czech Republic. The farms were divided into four groups according to breed and 2016 milk yield. Production costs increased during the period in all groups by 13% to 17% because of increasing milk yields, the associated higher feed consumption and increasing input prices. We observed a higher annual growth rate of costs per litre of milk on farms with above-average milk yields compared with the groups with below-average yields. The highest profitability before subsidies was achieved on farms with Holstein cows and above-average milk yields. Higher-income over feed costs were consistently observed in groups with higher milk yields. Because of low farm gate milk prices, profitability was significantly lower across all groups during 2016 than in other years.

Biodiversity of Vitis vinifera endophytes in conventional and biodynamic vineyardOriginal Paper

Maria Vrublevskaya, Thi Tra My Nguyenová, Lucie Drábová, Petra Lovecká, Blanka Vrchotová, Olga Ma»átková, Markéta Kuli¹ová, Irena Jaro¹ová Kolouchová

Czech J. Food Sci., 2023, 41(1):44-53 | DOI: 10.17221/200/2022-CJFS


Plants are permanently exposed to biotic and abiotic stress and have therefore developed intricate resistance mechanisms, consequently. These include the presence of microbial endophytes, which can promote plant growth and ensure better resilience against unfavourable conditions. These microorganisms colonising plant tissues can directly affect plant growth by producing phytohormones, antioxidants, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, or indirectly by the production of siderophores and antifungal agents. To the best of our knowledge, this is the first study devoted to assessing bacterial endophyte diversity and their plant growth-promoting properties in two utterly distinct vineyards in view of agricultural management (conventional, biodynamic) in the Czech Republic. With these different agricultural approaches, we hypothesised different numerical representations of bacterial endophytes acquired from vine shoots and leaves, which was not proved (P = 0.743, F = 0.129). A total of 470 distinct bacterial endophytes were isolated from the Vitis vinifera plants from the conventional and biodynamic vineyard and from which over 80% were identified by the matrix-assisted laser desorption-time-of-flight mass spectrometry (MALDI-TOF MS). In both vineyards, the dominant bacterial genus was Bacillus, followed by Pantoea, Pseudomonas and Staphylococcus. Plant-promoting endophyte properties varied with respect to the season and type of vineyard. The ability to produce indole-3-acetic acid (IAA) and ACC deaminase was higher in the biodynamic vineyard, in comparison with antioxidant activity, which was found in a higher proportion in isolates from the conventional vineyard.

Thinning effects on growth and occurrence of rotting in aspen standsOriginal Paper

Alina Nasibullina, Marieke van der Maaten-Theunissen, Ernst van der Maaten, Holger Fischer, Sven Wagner

J. For. Sci., 2023, 69(12):525-538 | DOI: 10.17221/103/2023-JFS

Poplar species such as aspen (Populus tremula L.) play a very important role in the forest formation process not only in Eastern European regions. Unfortunately, such aspen stands are often severely affected by fungal diseases, causing mainly core rot. In this study, the indirect effects of thinning on the phytosanitary condition of aspen by promotion of tree growth were investigated. Two thinning methods, manual (thinning from below) and mechanical thinning (schematic), were applied to young stands dominated by Eurasian aspen to study their effects on tree growth and health. All trees were measured at breast height and diameter frequency distribution was determined twice, i.e. three and 24 years after the beginning of the experiment. In addition, during the second measurement, tree-ring samples were obtained from individual trees to evaluate growth and wood decay damage. Neither manual nor mechanical thinning of aspen significantly increased its growth at the stand level, but positive effects on individual trees were observed in plots where mechanical thinning was applied. The thicker the trees, the less decayed they were. The analysis suggests that thinning in general should not be used to increase stand production, but the positive effects of mechanical thinning on individual aspens can be recommended to promote the growth of individual vigorous trees.

Seed yield, nutrient absorption and soil health as influenced by sowing time, nutrient levels and genotypes of garden pea (Pisum sativum L.)Original Paper

Anamika Chandel, Akhilesh Sharma, Parveen Sharma, Surinder Singh Rana, Ranbir Singh Rana, Shilpa

Hort. Sci. (Prague), 2023, 50(2):142-151 | DOI: 10.17221/138/2022-HORTSCI

The choice of variety, sowing time and optimum nutrition are important management options to optimise the seed yield. Accordingly, an experiment comprising of 18 treatments was conducted during the winter 2017–2018 and 2018–2019 in a factorial randomized block design, replicated three times to assess the response of pea genotypes (DPP-SP-6, Him Palam Matar-1 and Pb-89) to the seeding time (26th October and 10th November) and nutrient levels (0, 100 and 125% of the recommended NPK). The individual treatment effects revealed that early sowing, application of 125% NPK and DPP-SP-6 were significantly superior for the seed yield and related traits. The interaction effects indicated a significant response of the 125% NPK on the early sown crop for the seed yield, harvest index and NPK uptake. Significantly highest soil available NPK were obtained at 125% NPK with a respective increase of 41.54, 5.90 and 30.82 kg/ha, respectively, over the initial status. Early sowing of DPP-SP-6 with an application of 125% NPK is a better preposition for enhancing the productivity of peas.

The use of modern fermentation techniques in the production of traditional wheat breadOriginal Paper

Pavel Skøivan, Marcela Sluková, Ivan ©vec, Helena Èí¾ková, Iveta Horsáková, Eli¹ka Rezková

Czech J. Food Sci., 2023, 41(3):173-181 | DOI: 10.17221/39/2023-CJFS

A traditional Czech bakery white bread product is commonly made with directly mixed dough with the addition of yeast. In order to be able to reduce the salt content of the final product without compromising its acceptability to consumers, the dough was prepared with the addition of pre-ferments and wheat sourdoughs. The aim was to deepen the flavour background of the product and thus have a positive effect on its sensory properties. Wheat sourdoughs were prepared and tested using starter cultures and matured under different conditions. The resulting products were tested both sensorially and by means of precise physical methods.

High pressure processing for the production of vegetable baby puree with enhanced nutritional, microbial, and sensory qualitiesOriginal Paper

Farah Javed, Hafiz Muhammad Shahbaz, Waqas Ahmed, Habib ur Rehman

Czech J. Food Sci., 2023, 41(4):263-270 | DOI: 10.17221/2/2023-CJFS


High-pressure processing (HPP) was used as a post-processing treatment for vegetable puree. Microbiological, physicochemical, nutritional, and sensory analyses of puree were investigated at room temperature. HPP (600 MPa, 5 min) was compared with thermal treatment (117 °C, 30 min) and fresh samples. Treatments did not change pH or total soluble solids. For both methods treated samples exhibited a lower microbial count (< 1.0 log CFU·g–1) over storage, compared with fresh puree. During storage, other parameters, including total phenolic contents and antioxidants also demonstrated similar or better performance than controls (P < 0.05). Overall, HPP-treated puree received a higher sensory evaluation score. Thus, HPP can be used as an alternative processing technology to improve nutritional quality and microbial safety.

Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe diseaseOriginal Paper

Gang Jing, Youhua Yao, Likun An, Yongmei Cui, Yixiong Bai, Xin Li, Xiaohua Yao, Kunlun Wu

Czech J. Genet. Plant Breed., 2023, 59(4):263-277 | DOI: 10.17221/14/2023-CJGPB

To explore the role of WRKY transcription factors (TFs) in the resistance process of Qingke (Hordeum vulgare L. var. nudum Hook. f.), leaves of the leaf stripe disease-resistant variety Kunlun 14 and the susceptible variety Z1141 were sequenced by transcriptome sequencing (RNA-seq). A differentially expressed gene HvnWKRY1 was identified, and its disease-resistance function was preliminarily analysed. The result showed that the open reading frame (ORF) of the gene was 1 062 bp and encoded 354 amino acids. It contained the conserved WRKY domain (273–351) and belonged to the WRKY protein family. The phylogenetic tree results showed that HvWRKY1 was most closely related to Hordeum vulgare L. The WRKY family of Qingke, barley, maize and rice were divided into categories I, II, and III, among which HvWRKY1 was located in group III. Results of the quantitative real-time fluorescence PCR (qRT-PCR) showed that the expression of HvWRKY1 was significantly (P < 0.01) higher in leaf stripe infected leaves of Kunlun 14 than that of Z1141. In Arabidopsis thaliana transformed with HvWRKY1, resistance to Botrytis cinerea was enhanced. The RNA-seq analysis showed there were 824 differentially expressed genes (DEGs). Data of the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment indicated, that a plant-pathogen interaction pathway was enriched. This study is expected to provide a theoretical basis for further studies of functioning of  the Qingke gene HvWRKY1 in resistance to the leaf stripe disease.

Ultra-high-pressure technology for preservation of fresh aquatic foods: A reviewReview

Jiakun Peng, Chune Liu, Kaikai Bai, Shaohua Xing, Feng Liu

Czech J. Food Sci., 2023, 41(5):313-322 | DOI: 10.17221/87/2023-CJFS


Aquatic foods such as fish, shrimp, and shellfish are important human nutrient sources. However, aquatic foods spoil quickly during processing and storage due to spoilage bacteria and endogenous enzymes. Ultra-high-pressure (UHP) technology, as an advanced non-thermal treatment method, is an effective preservation technique for aquatic foods. The mechanism of UHP technology is to destroy non-covalent bonds by UHP, which leads to the change of cell membrane permeability and the destruction of high-level structure of proteins, making apoptosis and enzyme inactivation. The technology can effectively sterilise and preserve food's colour, taste and nutritional value. The paper provides an introduction to the working principles, types, sources and equipment and describes the progress of the research and application of UHP technology in pascalisation, enzyme inactivation, parasite inactivation and quality modification of aquatic products. Potential limitations and prospects of the technology are also outlined. We hope to lay the theoretical groundwork for using this technique in aquatic product processing and provide guidance for its application in industrial production.

Effect of novel carboxymethyl cellulose-based dressings on acute wound healing dynamicsOriginal Paper

A Kruzicova, M Chalupova, G Kuzminova, T Parak, J Klusakova, T Sopuch, P Suchy

Vet Med - Czech, 2023, 68(10):403-411 | DOI: 10.17221/89/2023-VETMED

The clinical implications and efficacy of newly developed modified cellulose materials were evaluated in an acute wound animal model. In the current study, sixty male rats were divided into four groups. A full-thickness circular excision wound was created in the suprascapular area. Newly developed matrices (acidic partially carboxymethylated cellulose; acidic partially carboxymethylated cellulose impregnated with a povidone-iodine solution) were applied in two test groups, while fifteen animals were used as a control group without any primary dressing. Aquacel Ag, a clinically used dressing, was selected as the reference material. To compare the efficacy in vivo, the wound size and production of selected cytokines and growth factors (TNF-α, TGF-β1, and VEGF), which play a key role in the healing process, were measured at two, seven, and fourteen days after surgery. The activity of matrix metalloproteinases 2 and 9, which actively participate in cell signalling and are essential for tissue remodelling, was determined in wound tissue by gelatin zymography. A positive effect of the newly developed dressing materials on the healing process, tissue granulation, and wound re-epithelialisation was demonstrated.


Modelling the tree height-diameter relationship of Macedonian pine (Pinus peuce Gris.) forests in North MacedoniaOriginal Paper

Vladimir Tanovski, Bratislav Matoviæ, Mihajlo Risteski, Pande Trajkov

J. For. Sci., 2023, 69(11):497-513 | DOI: 10.17221/68/2023-JFS

The most crucial individual tree variables in forest management are diameter at breast height (DBH) and height of trees (H). Projection of stand development over time relies on accurate height-diameter functions. The main aim of this paper is to define the best usable model for tree height prediction from diameter at breast height. We explore the place where the Macedonian pine was discovered in the Baba Mountain in the Pelister National Park in North Macedonia. Thus, we established 48 experimental plots (EP) with a circle shape, a radius of 12.62 m and an area of 500 m2 each. The EP were established in pure Macedonian pine stands in an elevation gradient between 950 m a.s.l. and 1 700 m a.s.l. Every tree in the EP was attributed with data for diameter at breast height, tree height, and stand characteristics data (elevation, slope, aspect, coordinate) as well. For predicting the tree height, we used 40 models from many authors which are the most usable in forest practice. Also, we prepared evaluations and tests for all models, in order to choose the best responsive model for the Macedonian pine forest. For predicting the best tree height model for Macedonian pine, we decided on Mamoun's equation, with a high correlation value of 0.85 with 73% out of the observed data. Also, this model showed a lower root mean square error of 32.65, a lower model prediction accuracy of 6.77, and a lower mean absolute percent error of 11.73%. Finally, it can be concluded that the nonlinear connection between DBH and tree height is the most responsive regression model.

Changes in the fragmentation and ecological stability of the Morava River floodplain forest in the course of the 20th century

I. Machar

J. For. Sci., 2009, 55(3):127-136 | DOI: 10.17221/98/2008-JFS

This paper presents the results of an analysis of the changes in the fragmentation and ecological stability of the floodplain forest geobiocoenoses in the Protected Landscape Area Litovelské Pomoraví, Czech Republic. Using GIS methods, it was determined that the fragmentation within the study area had increased slightly and the ecological stability of the landscape had decreased slightly between the years 1938 and 2006, although the latter remained on a fairly high level. The data reflect the anthropogenic conditionality of the floodplain forest ecosystem and show that an anthropogenically conditioned geobiocoenosis may attain a relatively high level of ecological stability, this being particularly characteristic of floodplain forest geobiocoenoses in Central Europe. The results of the study contribute to the documents that will be used in drawing up a management plan for the locality important at the European level which is a part of the Natura 2000 system.

The economic well-being of smallholders and challenges during COVID-19 pandemic: A reviewReview

Nurul Atiqah Binti Mohd Suib, Norlida Hanim Mohd Salleh, Mohd Fazim Ahmad

Agric. Econ. - Czech, 2023, 69(1):35-44 | DOI: 10.17221/344/2022-AGRICECON

The challenges smallholders face in agricultural management between developed and developing countries differ and can also impact the smallholder's well-being. The purpose of this study is to identify factors that affect the well-being of smallholders. We conducted a systematic literature by applying the Preferred Reported Item for Systematic Review and Meta-Analysis (PRISMA) and extracted 30 articles from Scopus and Web of Science. These articles show three main factors that drive well-being: technology, optimal resource, and insurance, and two additional factors: market pricing and tax policy. Findings show that all the above factors will first impact economic well-being and, subsequently, social well-being. When COVID-19 struck, the smallholder's well-being was also affected in addition to the destabilised food chain. Travel restrictions have led to a labour shortage. The smallholders had to turn to e-commerce to sell their products. They also incurred losses and experienced difficulties in procuring agricultural intermediate inputs. The absence of agricultural insurance worsened the impact on the well-being of the smallholders. To safeguard the well-being of these smallholders, the study suggests that stakeholders should encourage local labour participation, increase investment in human capital, adopt e-commerce in marketing, offer agricultural insurance schemes against various disasters and continuously provide or increase subsidies to smallholders.

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A reviewReview

S Kumar, MS Chaves, AFB da Silva, WG Vale, STR Filho, JC Ferreira-Silva, LM Melo, VJF Freita

Vet Med - Czech, 2023, 68(2):45-56 | DOI: 10.17221/48/2022-VETMED


Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes.

Timer versus moisture sensor-based irrigation control of soilless lettuce: Effects on yield, quality and water use efficiencyOriginal Paper

F.F. Montesano, M.W. van Iersel, A. Parente

Hort. Sci. (Prague), 2016, 43(2):67-75 | DOI: 10.17221/312/2014-HORTSCI

The study compares the effects of: timer ('Timer') and soil moisture sensor-controlled irrigation on soilless lettuce; two volumetric water content (Θ) thresholds for irrigation (0.30 ('Θ = 0.3') and 0.40 m3/m3 ('Θ = 0.4')). The most nutrient solution (NS) was applied in 'Timer' where the lowest water use efficiency was observed, with 17 and 42% less NS used in 'Θ = 0.4' and 'Θ = 0.3', respectively. Irrigation volumes followed the plant water needs in the sensor-controlled treatments, with little or no leaching, while 18% of leaching was recorded in 'Timer'. Plants in 'Timer' and 'Θ = 0.4' had higher fresh weights (24%) and leaf area (13%) than plants in 'Θ = 0.3'. Similar dry weight was observed among treatments but percentage of dry matter was 20% higher in 'Θ = 0.3'. Gas exchanges and leaf tissues chemical composition were similar in all treatments, but nitrate concentration was lower in the 'Θ = 0.3' plants. Precision sensor-controlled irrigation based on Θ measurements is an effective tool to increase the overall water use efficiency and to improve the quality of soilless-grown lettuce by acting on the substrate moisture level.

Studies on biogas production by anaerobic process using agroindustrial wastesOriginal Paper

P. Elaiyaraju, N. Partha

Res. Agr. Eng., 2016, 62(2):73-82 | DOI: 10.17221/65/2013-RAE

This study investigated the effect of factors namely temperature, pH, substrate concentration on sago and tannery effluents by the anaerobic digestion process for biogas production. Response surface methodology with the Central Composite Design (CCD) experiments verified that the biogas production rates were mainly affected by operating temperature, pH, and substrate concentration. The experiments were carried out by two distinct effluents at different organic loading rate under mesophilic range of temperature 31-33°C. Co-digestion was carried out for a period of 21 days. The gas produced was measured by the liquid displacement system. Meanwhile, the highest biogas yields - 80% of CH4 and 20% of CO2 -produced in the combined effluent were confirmed by the Gas Chromatography (GC) analysis.

Forest visitors' opinions on the importance of forest operations, forest functions and sourcesof their financing

L. ©i¹ák

J. For. Sci., 2011, 57(6):266-270 | DOI: 10.17221/135/2010-JFS

The survey was conducted in three selected areas of the Czech Republic in 2008, with the results processed in 2009. Forests visitors received inquiry forms (face to face interviews, random sample). The total number of visitors on 8 survey days was 7,369. The total number of filled-in questionnaires in the three areas was 1,122. Tree planting and tree protection are considered as the most important forest operations, followed by road and stream bank maintenance. On the contrary, timber transport and harvesting are considered as the least important activities. The nature-protecting function is considered as the most important forest function, followed by soil-conservation, climatic, hydrological and health (recreational) functions. Timber production and non-timber production are the least important functions according to the respondents. 20% of respondents claimed that increased costs needed to improve non-market forest functions, used by the visitors, should be partially or fully financed from the timber sales revenues, while only 6.5% of respondents say the costs should be partially or fully financed from payments by the users of forest functions.

Effects of irrigated agriculture on water and soil quality (case perimeter Guelma, Algeria)Original Paper

Nora KACHI, Slimane KACHI, Houria BOUSNOUBRA

Soil & Water Res., 2016, 11(2):97-104 | DOI: 10.17221/81/2015-SWR

In Algeria, more than 20% of irrigated soils are concerned by the problem of salinity. In the perimeter of Guelma, the results showed that the soils have low salinity despite of high electrical conductivity (1244 µS/cm) of irrigation water and increased organic matter, nitrogen, potassium, and phosphorus contents. It should be noted that the soils had exhibited higher salinity and low organic matter content before starting the irrigation in 1973. The reduction in the salt content is due to the leaching of salts from percolated water after the intensive irrigation; however, the increase in the content of organic matter can be explained by the accumulation of the organic matter transported by irrigation water, conditions of waterlogging, and types of crops (vegetable/cereals). As to the quality of groundwater, nitrate is the dominant nitrogen component entering groundwater and it is very alarming that by 45% of water sources (springs, wells, and boreholes) the nitrate contents are over 50 mg/l. It is a serious problem because it serves as a drinking water supply for the local population. Moreover, this water is highly mineralized (electric conductivity > 1000 µS/cm) because of the intensive use of artificial fertilizers and to the poor quality of water irrigation. The contamination by organochlorine pesticides is below the value admitted by the WHO for water resources. Endosulfan is the most dominant active ingredient with an average concentration of 0.066 µg/l.

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