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One of the many factors that leads to soil erosion is rainfall erositivity, which is a basic physical factor enabling us to understand the geomorphological processes that take place in a basin. Results worldwide have shown that the erositivity R factor of the Universal Soil Loss Equation (USLE) has a high correlation with soil loss. In the past there have been few pluviometers capable of recording rainfall with continuous measurements. As a result of this lack of accuracy in the available series of rainfall intensity data, the calculation of the R factor has been restricted for a long time and various simplified models were developed on an international scale that relied on information obtained from existing stations. However, the modernisation of stations over the last few decades has provided to be a valuable tool for validating models, as well as for designing others that are more hardwearing and correlate better with the available information. In this paper, we have calculated the rainfall erositivity R factor for a semiarid basin in SE Spain using the formula developed in the USLE model for a series of 20 years of rainfall with 5-minute intervals, obtaining the mean R factor value of 620 MJ/ha∙mm/h per year and maximum values of up to 6000 MJ/ha∙mm/h per year. In addition, a comparative analysis of various simplified expressions was carried out to obtain the R factor. To obtain this value, we came up with a simplified equation based on annual maximum daily rainfall and average monthly rainfall, which resulted in a correlation coefficient of r = 0.936 and a P-value of 0.033 for the basin under study. Thus, from this structure of the equation we have compiled a series of parametric maps which enable us to calculate the R factor from any position within the basin under study.

The impact of vector density and timing of infestation on potato were investigated. Healthy potato plants at different growth stages (4, 5, and 7 weeks after germination) were exposed separately to four different B. cockerelli densities (0, 5, 20, and 40 psyllids per cage) in field cages and Zebra chip (ZC) symptoms, leaf photosynthetic rates, tuber yield, and total nonstructural carbohydrate accumulation in leaves and tubers of healthy and B. cockerelli-infested plants were monitored. Potato psyllid nymph and egg populations reached a seasonal peak at 6 weeks after the exposure to insect. Younger plants at 4-week growth stage after germination were more susceptible to B. cockerelli infestation and ZC expression than older plants. As few as five B. cockerelli adults were enough to transmit the ZC pathogen and cause ZC expression both in foliage and tuber. At the density of 20 psyllids per cage, more than 50% of plants showed ZC symptoms in tubers. Furthermore, B. cockerelli infestation reduced leaf photosynthesis rates (P_n), resulting in less starch and more reducing sugars in tubers, and hence reduced tuber weight and yield, especially when psyllid infestation occurred at the early growth stages. The results indicate that early B. cockerelli infestation of younger plants was associated with more severe ZC expression in both foliage and tubers, leading to earlier dead plants. The data suggest that strategies for controlling B. cockerelli during early potato crop development could thus lessen the severity of ZC development.

Physical properties of soils have a significant influence on their water regime and should be considered when selecting suitable agricultural crops for particular sites, taking into account the crop productivity and its water requirements. Mean daily rates of actual evapotranspiration (ETa) were obtained by collation of measured or otherwise estimated 10-min values for the years 2004, 2006 and 2009 for a partially tile-drained agricultural experimental catchment in the Bohemo-Moravian Highland (Czech Republic). ETa was measured using the Bowen ratio (β) and energy balance (BREB) method at four weather stations located on different soil types (Stagnosols, Cambisols) and terrain relief positions (defined with respect to the groundwater recharge and discharge zones) over different crops (cereals, oil rape and permanent grassland). A systematic influence of soil properties on the evapotranspiration rate was more pronounced during the periods of limited transpiration (soil drought, crop maturity), when the average daily ETa was significantly lower and the corresponding β significantly higher over coarser-textured soils (shallow Haplic Cambisols), namely (year-ETa (mm/day)/β): 2004 - 1.75/1.66; 2006 - 2.44/0.93; 2009 - 2.60/0.81), than over finer-textured soils (Stagnic Cambisols and Haplic Stagnosols), namely: 2004 - 2.92/0.97; 2006 - 3.06/0.44; 2009 - 3.42/0.39). When the transpiration was not limited by soil water deficit, it acted as an equalizing factor smoothing down evapotranspiration from heterogeneous soil areas, whereby the effect of the soil physical properties was masked. With regard to soil water regime and evapotranspiration, the tile-drained Stagnic Cambisol lands behaved similarly as non-drained Haplic Cambisols. The effects of land use and of the terrain relief position could not be tracked independently, because the permanent grassland was situated in the wetter and texturally heavier parts of the catchment and the positions of the recharge/discharge zone coincided with the positions of texturally lighter/heavier soils, respectively.

Peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae), has been a serious pest in the last decade attacking a wide range of fruits in Egypt. The toxicity of Malathion, Diazinon, Methoxyfenozide, and Lufenuron to adult males and females of Bactrocera zonata was studied under laboratory conditions. Diazinon was the most toxic among the tested compounds followed by Malathion, Lufenuron and Methoxyfenozide to Bactrocera zonata at 24 h post treatment, the respective LC₅₀ values were 0.20 ppm, 0.48ppm, 8.97ppm, and 9.73ppm for males and 0.26 ppm, 0.91ppm, 11.26ppm, and 14.12ppm for females. At 48 h post treatment Diazinon was the most toxic followed by Malathion, Methoxyfenozide and Lufenuron to Bactrocera zonata, LC₅₀ values were 0.09ppm, 0.34ppm, 1.60ppm, and 1.88 ppm for males and 0.14 ppm, 0.44ppm, 1.68ppm and 2.17 ppm for females. At 72 h post treatment Diazinon was the most toxic followed by Malathion, Lufenuron and Methoxyfenozide to Bactrocera zonata, LC₅₀ values were 0.02 ppm, 0.13ppm, 0.22ppm and 0.51ppm for males and 0.07 ppm, 0.16ppm, 0.55 ppm and 0.62 ppm for females. It is observed that LC₅₀ values for treated adult females increased more than in the treated adult males at 24 h, 48 h, and 72 h post treatment. It means that the adult males were more susceptible to the tested insecticides than the adult females.

So far, only the chronic effect of sulforaphane (SF) on metabolism was examined. This study sheds more light on SF potential ability of regulating lipid, carbohydrate, and hormonal metabolism during its acute action in in vivo and in vitro conditions. In the in vivo trial, rats were given once intragastrically 10 or 20 mg/kg of SF and were decapitated 4 h after the single intragastric treatment. The serum and the liver were collected to assay lipid, carbohydrate, and hormonal parameters. Additionally, we evaluated the acute direct in vitro action of SF (1.5 h) on basal and insulin-stimulated lipogenesis and basal and epinephrine-induced lipolysis in isolated primary rat adipocytes at 1µM, 10µM, and 100µM concentrations. The SF hormonal action was dose-dependent. In the in vivo trial, the higher dose evoked a significant insulin release (P ≤ 0.01) and showed a tendency to limit the secretion of leptin from adipocytes compared with the control animals. Surprisingly, two applied SF doses did not cause any changes in serum glucose level and liver glycogen content. Both SF doses reduced HDL- and increased LDL-cholesterol level (P ≤ 0.05), evoked a drop of liver triacylglycerol content (P ≤ 0.05) compared with the control rats. In the in vitro study, only 100µM SF evoked elevation of basal- and epinephrine-induced lipolysis and inhibition of basal- and insulin-induced lipogenesis in comparison with the control (P ≤ 0.001). SF adipocyte influence was independent of epinephrine and insulin action. Recapitulating, SF exhibited a tendency towards limiting lipid synthesis in adipocytes as well as in the liver, possibly via Nrf2 pathway. The disturbance in the LDL- to HDL-cholesterol ratio and dose-dependent increase in insulin concentration at normal glycaemia were connected probably with the SF capability to generate temporarily ROS in the pancreas and in the vascular endothelial cells in in vivo trials.

This study is focused on substitute European larch stands in the Krušné hory Mts. (northern part of the Czech Republic). Research was conducted within larch thinning experiment Kalek (780 m a.s.l. in the category Piceeto-Fagetum oligo-mesotrophicum - Calamagrostis villosa). Results showed that the aboveground biomass of the investigated substitute unthinned larch stand represented approximately 102 thousand kg of dry matter per ha at the age of 20 years. Stemwood (ca 59%) is the most important part of the aboveground biomass. Needles, live and dead branches accounted approximately for 6%, 17% and 11%, respectively, and stem bark only for 7%. At the age of20 years, the investigated substitute unthinned larch stand accumulated: nitrogen - 307 kg, phosphorus - 21 kg, potassium - 136 kg, calcium - 122 kg, magnesium - 53 kg per hectare. Thinning with consequent removal of aboveground biomass may result in nutrient losses. Especially, the removal of whole tree biomass by thinning for chipping in areas previously degraded by acid deposition may result in calcium and magnesium deficiency because of their low content in forest soil. On the other hand, thinning supported faster growth of trees left after thinning and consequently faster biomass and nutrient accumulation. Our results supported the recommendation that the use of biomass from thinning for chipping should be limited to stemwood only and the remaining aboveground biomass (mainly needles and branches) should be left in the forest ecosystem for decomposition in conditions of the historically disturbed area of the Krušné hory Mts.

Different variants of regeneration felling or different light conditions (total site factor 15-95%) and weed control were evaluated in relation to the 4-year development of individuals of regenerated sessile oak. The regeneration density is not dependent on light conditions. The highest mortality occurs on the open area of clear felling. For a certain time in youth, it is possible to consider oak as a shade-tolerating species. With the increasing light intensity the diameter and height growth of oak seedlings increases proportionally, being the highest at 100% light intensity. However, to support at least medium-fast growth, the species requires minimally 50% light intensity (smaller closed clear-felled areas). On the other hand, more extensive unprotected cleared areas appear to be quite unsuitable at the initial stages of regeneration. The positive effect of weed control on the success and growth of natural regeneration is questionable. We recommend developing the regenerated stand by means of a series of small-area (about ≤ 0.3 ha) two-stage felling with a medium-long regeneration period (15-20 years).

In this study, the effects were investigated of salinity, foliar and soil applications of humic substances on the growth and mineral nutrients uptake of Corn (Hagein, Fardy10), and the comparison was carried out of the soil and foliar applications of humic acid treatments at different NaCl levels. Soil organic contents are one of the most important parts that they directly affect the soil fertility and textures with their complex and heterogenous structures although they occupy a minor percentage of the soil weight. Humic acids are an important soil component that can improve nutrient availability and impact on other important chemical, biological, and physical properties of soils. The effects of foliar and soil applications of humic substances on the plant growth and some nutrient elements uptake of Corn (Hagein, Fardy10) grown at various salt concentrations were examined. Sodium chloride was added to the soil to obtain 20 and 60mM saline conditions. Solid humus was applied to the soil one month before planting and liquid humic acids were sprayed on the leaves twice on 20^th and 40^th day after seedling emergence. The application doses of solid humus were 0, 2 and 4 g/kg and those of liquid humic acids were 0, 0.1 and 0.2%. Salinity negatively affected the growth of corn; it also decreased the dry weight and the uptake of nutrient elements except for Na and Mn. Soil application of humus increased the N uptake of corn while foliar application of humic acids increased the uptake of P, K, Mg,Na,Cu and Zn. Although the effect of interaction between salt and soil humus application was found statistically significant, the interaction effect between salt and foliar humic acids treatment was not found significant. Under salt stress, the first doses of both soil and foliar application of humic substances increased the uptake of nutrients.

High-value native timber species are being promoted in reforestation in Southeast Asia. However, slow growth during early establishment, coupled with poor soil fertility, poses challenges for promoting tree planting programs. Field trials were undertaken on agroforestry land in Thailand to examine the efficacy of reforestation treatments. The first trial examined the effect of applying microorganisms and fertiliser on directly seeded Acacia mangium Willdenow, Dalbergia cochinchinensis Pierre and Xylia xylocarpa (Roxburgh) Theobald. After 20 months, a mixed inoculum of arbuscular mycorrhizal and ectomycorrhizal fungi improved survival and basal diameter of D. cochinchinensis by 15 and 43%, respectively. The co-inoculation of arbuscular mycorrhizal fungi and N2-fixing bacteria improved survival by 17%. The second trial investigated the effects of fertiliser and a water retention polymer on directly seeded A. mangium, Afzelia xylocarpa (Kurz) Craib, D. cochinchinensis, Eucalyptus camaldulensis Dehnhardt, Sindora cochinchinensis Baillon and X. xylocarpa. Height was improved by 40% at 20 months. Our findings suggest that reforestation is viable, provided that suitable treatments are used. These principles can be applied for reforestation of nutrient-impoverished soils of continental Southeast Asia.

The chemical composition and content of fatty acids in flaxseed and spelt flour were evaluated. The ratio of ω-6/ω-3 essential fatty acids is also analysed in spelt pasta and pasta with 0, 10, and 20% of flaxseed flour. Flaxseed flour has a better fatty acid profile than spelt flour, with low levels of saturated fat (approximately 8.99 g/100 g of flour) and a high concentration of linolenic acid (57.20 g/100 g of flour) and lower content of linoleic acid (15.98 g/100 g of flour), as well as superior ω-6/ω-3 ratio of 1 : 4. Flaxseed flour in pasta positively contributes to the daily intake of essential fatty acids recommended by nutritionists and the improvement of ω-6/ω-3 ratio. Although a new product is worse in texture quality, it will be acceptable to consumers who want to change their habits related to diet and enrich it with functional components.

In this study, the immunohistochemical features of primary hepatocellular carcinomas and their metastases in visceral organs, including the lungs, spleen and kidneys were examined using antibodies against carcino-embryonic antigen (CEA) cytokeratin (CK) 7 and 20, CD4, CD8, minichromosome maintenance protein 3 (MCM3), vimentin, and alpha 1 foetoprotein (AFP). In addition, Mallory's connective tissue stain, van Gieson's stain and Gomori methenamine silver stain were used. The study was performed on liver samples collected post mortem from five mixed-breed dogs aged 9-12 years. The tumours were classified according to the World Health Organization Classification of Tumours. Strong expression of MCM3 and AFP was found in the hepatic cancer cells and in the metastases to the lungs, spleen and kidneys. The primary tumours and metastatic foci did not react positively with the anti-CD4, anti-CD8, CEA, CK7 and CK20 antibodies. The connective tissue in the primary tumour and the metastases showed a positive reaction to vimentin. Canine hepatocellular carcinomas that metastasise are highly-malignant well-differentiated tumours that produce AFP and trace amounts of both carcinoembryonic antigen and cytokeratin. Therefore, the metastasis resembles the primary tumour and has a common phenotype and genotype with the primary tumour.

The development of electronic components in engine regulation leads to the possibility of obtaining a large amount of parameters of control processes. Nowadays, these data can be read in an easy way due to the properties of used networks. The data obtained from the data bus must be processed carefully; otherwise, there is a risk of erroneous results. The article presents the results of measurements performed on four tractors. We focused on comparing the fuel consumption of engines and the accuracy of flow measurement readings from the CAN-Bus of these tractors. Even the same engines may show considerable differences in fuel consumption, taken from the CAN-Bus. The maximum deviation of the measured values ranged up to around 40%. However, the value of about 41% was measured in the range of fuel consumption of around 10 kg/h, the error of that tractor at the consumption of about 40 kg/h fell to 1.5 kg/h. A significant difference occurred in the tractor with a 235 kW engine. The consumption in the range of 50 kg/h showed an error of 8.4 kg/h. An improvement of the results can be achieved using derived regression functions. Based on our experience with the measurement of other types of tractors, we know that a similar situation with the accuracy of CAN-Bus data is also an issue in other categories of vehicles.

Earthworms dominate the soil biota, and different structural and functional features of their biology and ecology have been studied and exploited to evaluate their contributions as ecosystem services. Due to their feeding ecology, burrowing and casting activity, earthworms are involved in the nutrient cycles, and therefore it is opportune to be considered when the biogeochemical cycles of the terrestrial ecosystems are analysed. All structural, microbiological and biogeochemical impacts of earthworms in soil start with their feeding and digestive functions, which end in casting. The casting activity consisting of the excretion of the ingested soil and organic matter after digestion processes depends on earthworm feeding behaviours and ecology, even described in the current literature as a new ecological feature: the casting ecology. The complexity of the chemical relationships occurring inside earthworm casts between main nutrients (organic carbon, nitrogen, phosphorus, potassium, calcium) highlights the complexity of the biogeochemical cycles and the great earthworms’ contribution to these cycles in the ecosystems towards a better understanding of the soil sustainability through the soil biodiversity contribution. Due to this great contribution, the earthworms’ casts should be included as indicators in the integrative conservation management of the ecosystems, as a re-thinking of the concept of ecosystem sustainability.

Sustainable global wheat production requires wheat varieties, that are sufficiently resistant to the main wheat diseases. The economically important fungal pathogens worldwide include powdery mildew (PM), yellow rust (YR), leaf rust (LR) and blotch causing pathogens including Septoria nodorum blotch (SNB) and Septoria tritici blotch (STB). Here, we present the evaluation of winter wheat varieties of diverse origin against the prevalent local populations of PM, YR, LR, STB and SNB under natural infection conditions through image-based phenotyping in two consecutive years (2019 and 2020). We found several varieties to be resistant against multiple diseases. Following the association mapping, we obtained a total of 206 marker trait associations for all the parameters scored which were condensed to 79 quantitative trait loci (QTLs) (eight QTLs for PM, 25 QTLs for LR, 11 QTLs for YR, 19 QTLs for SNB and eight QTLs for STB) based on the linkage disequilibrium among the molecular markers. The known genes present at these QTLs are discussed in detail. The varieties resistant to multiple diseases, identified with the QTLs and molecular markers can be considered as elite raw material for future wheat breeding.

This study has evaluated the genetic characteristics and wheat processing-related properties of four Korean wheat landraces (KWLs). The KWLs were found to possess the vernalization alleles vrn-A1, vrn-B1, and Vrn-D1 and the photoperiod alleles Ppd-A1b, Ppd-B1b, and Ppd-D1a. The Korean cultivated variety Keumgang also shared these alleles with the exception of vrn-D1. With regard to grain hardness, KWL 2 was shown to possess Pina-D1a and Pinb-D1b like Keumgang, while other KWLs were classified as carrying Pina-D1a and Pinb-D1a. All KWLs were found to be non-waxy, carrying the alleles Wx-A1a, Wx-B1a, and Wx-D1a. With regard to the polyphenol oxidase (PPO) genes, all four KWLs carried low-activity alleles, in contrast to the Keumgang sample. The assessment of physicochemical properties revealed that KWL 1, 3, and 4 had a higher amylose content but a lower protein content than KWL 2 and Keumgang. In tests of solvent retention capacity KWL 1 and KWL 2 exhibited the lowest and highest values, respectively, for all four solvents used in the tests. With regard to the dough properties, the results of Mixolab analysis indicated a faster starch gelatinisation in KWL1, while in KWL 2 a high water absorption and the longest dough development and stability times were found. KWL 3 and 4 exhibited similar dough behaviours. Principal component analysis of the four KWL lines revealed distinct clustering based on their physicochemical and dough-related traits.

This review examines bacteriophages as alternatives to antibiotic growth promoters (AGPs) in broiler chickens, with a unique focus on effects observed in studies without experimental bacterial challenges. Driven by global antimicrobial resistance (AMR) concerns and sustainable poultry production demands, phage supplementation, a targeted strategy, potentially improves growth performance and gut health by preserving beneficial microbiota. This literature analysis assesses phage efficacy in healthy broilers under non-challenge conditions, evaluating key variables such as phage concentration, delivery, and targets, as well as outcomes such as feed conversion ratio (FCR) and gut health markers. Crucially, this review extends beyond efficacy to explore phage immunomodulatory capabilities, outlines optimisation strategies, and addresses risks and mitigation. Key findings show mixed efficacy of phages in non-challenged broilers: significant improvements in FCR and gut health were generally observed with high phage concentrations (e.g., ≥10⁸ PFU/g) and continuous delivery protocols, whereas lower doses yielded inconsistent or minimal benefits. Furthermore, choosing which bacteria phages target – for example, aiming at pathogens like Salmonella or managing common gut bacteria such as E. coli – greatly impacts outcomes. While phages show considerable potential as AGP alternatives, the review highlights that further research is vital to resolve inconsistencies, standardise protocols, and understand host genetic and environmental influences to optimise their commercial application.

Order-based agriculture aligns with the fundamental requirements for the high-quality development of modern agriculture in the new era. This study investigates how social capital influences the participation of family farms in order-based agriculture across the mountainous regions of southwestern China. Drawing on survey data from 557 farms and employing logit and Karlson-Holm-Breen (KHB) mediation effect models, the research reveals that social capital significantly promotes participation. Specifically, stronger social networks, heightened trust, and established norms are associated with increased engagement. While access to information mediates this relationship, the direct effect of social capital remains predominant. These findings underscore the importance of enhancing both social capital and information accessibility to encourage greater involvement among family farms. Furthermore, targeting younger and middle-aged farmers engaged in single-product production is crucial for promoting sustainable development.

More than 50% of agricultural land is threatened by water erosion in the Czech Republic. With respect to soil erosion, maize (Zea mays L.) belongs to the most problematic crops; one of the possibilities to increase protection against erosion is intercropping. In this study, we attempted to find out the effects of individual intercrops and their mixtures (sown 4-6 weeks after sowing maize) or a mixed culture (maize plus lupine) on the soil losses and surface runoff in the period 2019-2021. The study was realised in a sugar beet growing region (Haplic Luvisol); a field rainfall simulator was used. From the used variants with Lolium perenne L., Trifolium repens L., Vicia villosa Roth, Lolium multiflorum Lam., Festuca arundinacea Schreb., Triticum aestivum L. or a mixture (Vicia villosa plus a Trifolium hybrid diploid), the variants with Lolium perenne, Lolium multiflorum, Vicia villosa or Vicia villosa plus the Trifolium hybrid diploid, established between the maize rows (hybrid maize, cultivar Walterinio) on May 27, were the most efficient in case of both the soil losses and runoff reductions in the year 2019. For example, Triticum aestivum between the maize rows mostly reduced the soil losses and the surface runoff was similar (or higher) compared with the control (maize without any intercrop). The variant with Trifolium repens had mostly higher (or similar) soil loss values (compared with the control); in this variant, the runoff was lower compared with the control. We proved our hypothesis with regards to the higher reduction in the soil losses than with the runoff in the variant with Lolium perenne. The results from the years 2020 (the used variants with Lolium multiflorum, Secale cereale L., Trifolium incarnatum L., Phacelia tanacetifolia Benth., Lolium multiflorum plus Trifolium incarnatum, Lolium multiflorum plus Vicia pannonica Crantz) and 2021 (the variants with Lolium multiflorum, Lolium multiflorum - early sowing, Secale cereale, Trifolium incarnatum, Phacelia tanacetifolia, Lolium multiflorum plus Trifolium incarnatum, a mixed culture = maize plus Lupinus albus L.) showed the variants with Trifolium incarnatum, the mixture (Lolium multiflorum plus Trifolium incarnatum), Phacelia tanacetifolia (in the year 2020) or the mixture (Lolium multiflorum plus Trifolium incarnatum) and a mixed culture (maize plus Lupinus albus) (2021) had the most positive effect - the soil loss and surface runoff values were lower when the maize was > 2 m compared with the maize < 1 m. The results obtained in the period 2019-2021 showed the grasses were the most efficient in decreasing the soil losses when the maize was < 1 m and when the maize was > 2 m with the used mixtures.

Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism, we reconstructed a mapping segregation population (BC₁F₁) of 12 000 plants. Using the population and polymorphism of simple sequence repeat (SSR) molecular markers, we finally mapped Rf(fa) between the two SSR molecular markers MM2000 and RM25658, within a 78.87 kb region. By de novo sequencing of a restoring line of CMS-FA hybrid rice, we obtained the genomic sequence of the mapping region, which provided the basis for the prediction of the candidate gene(s) of the target gene and for the comparison of genomic sequence differences between wild and cultivated rice. Within the mapping region, the genomic sequence of the wild rice was significantly different from that of cultivated rice. There were ten genes in the final mapping region. A pentatricopeptide repeat (PPR) protein gene was predicted as the candidate gene of Rf(fa). Our results laid a solid foundation for the final cloning and molecular mechanism analysis of the gene. The identified molecular markers tightly linked to Rf(fa) will facilitate the marker assisted selection in breeding of CMS-FA hybrid rice.

The genetic characterisation of naturally occurring almond genotypes can guide the selection of genetic resources to be used in the breeding programme. Therefore, this study aims to assess the genetic diversity and population structure of almond germplasm comprising 50 accessions naturally occurring in Van, Türkiye, along with two commercial varieties (Pabuç and Dokuzoğuz). Thirteen inter-primer binding site (iPBS) retrotransposon markers generated a total of 102 bands, of which 95 were polymorphic. The average polymorphic band number per marker was 7.3, with a range of 5 to 13. A formula yielding a maximum of 0.5 resulted in polymorphic information content (PIC) values between 0.27 and 0.43, with a mean value of 0.36. Unweighted pair group method algorithm (UPGMA), principal coordinate analysis (PCoA), and STRUCTURE analysis, based on Bayesian clustering analysis, yielded consistent results, indicating that local populations (Akdamar and Çarpanak) were distinctly grouped, while commercial accessions were clustered with Çarpanak accessions. The diversity metrics and classification analysis utilising 13 iPBS-retrotransposon markers demonstrated that the iPBS-retrotransposon marker system possesses significant promise for evaluating the genetic variety and population structure of almonds.

The main goal of the paper is to evaluate the impact of the Russian import ban on the development of agricultural exports from EU member states. The study is based on a time-series analysis of empirical statistical indicators. The analysed period is between 2009 and 2019. The source of data for individual analyses is UN Comtrade (2021). The evaluation of export performance is extended by hierarchical cluster analysis. The study quantifies the effects of the import ban on the EU and individual member states through two scenarios. Scenario 1 is based on the cumulative loss of the value of exports. Scenario 2 assumes a continuous development of the value of agricultural exports. Based on the results, it is possible to confirm that the application of the Russian food import ban had a significant impact on EU countries. The impact of sanctions varies across EU countries. Four specific clusters could be identified in the period under investigation. In the period after the ban, the distribution of individual countries among individual clusters changed significantly. The applied ban could be understood not only as an attempt at counter-sanctions. Import restrictions also aim to reduce Russia's dependence on food imports and promote national food security.

A patellar luxation and concomitant cranial cruciate ligament rupture is a common pathology in dogs. Diagnosis is based on clinical evidence of a patellar luxation and stifle joint instability. However, diagnostic imaging is required to assess the number of skeletal deformities and signs of instability. Surgical options include both soft tissue and osseous techniques, although, in most cases, a combination of multiple procedures is necessary to correct the patellar luxation and restore the stifle joint stability. Complication rates are generally low, but can include reluxation and implant-associated complications. This article describes the patellar luxation and cranial cruciate ligament rupture signs in dogs, including the clinical presentation and diagnosis, and discusses current treatment options.

The herbicidally active Botrytis strain HZ-011 was isolated from naturally infected leaves of Rumex patientia L., and previous studies found that strain HZ-011 exhibits highly effective herbicidal effects against the broadleaf weeds Amaranthus retroflexus L., Elsholtzia densa Benth, Malva crispa, and Chenopodium album L. In this study, the active components of strain HZ-011 were isolated, purified, and structurally characterised using silica gel column chromatography, thin-layer chromatography, and high-performance liquid chromatography. The extraction test was carried out using four organic solvents with different polarities, and ethyl acetate was ultimately used as the extractant for the bulk preparation of the crude product of the active substances. Thin-layer chromatography yielded 10 different fractions and 26 components, and components B₃ and E₁ showed pathogenic effects on C. album. High-performance liquid chromatography (HPLC) of compound components B₃ and E₁ yielded three fractions with larger signal values and peak areas, and raw measurements of them revealed that component 3 was active. By analysing and comparing the spectral data of component 3 from ¹H-NMR, ¹³C-MNR, ESIMS, and EIMS, component 3 was identified as dibutyl phthalate. The activity of the individual compounds was verified. The results of this study lay a theoretical foundation for the discovery and exploration of lead compounds. They will also provide a theoretical basis for developing this strain into a microbially-derived herbicide.

For 2 years (2019-2020), a field experiment to test the activities of different fertilisation schemes on the yield, colour and health status of five different grass mixtures for football pitches was conducted. Two grass mixtures were composed of different varieties of perennial ryegrass, one mixture was composed of varieties of perennial ryegrass and Kentucky bluegrass, one mixture consisted of the species Lolium perenne, Festuca rubra, Festuca arundinacea and Poa pratensis, and the seeds of only one variety of perennial ryegrass were sowed in one plot. Three different fertilisation schemes were included in the experiment. The first scheme (A) contained an inorganic fertiliser with added soil improvers, the second scheme (B) included an organic fertiliser with added soil improvers and the third scheme (C) was composed of an inorganic fertiliser. According to this study, the grass yield largely depends on the soil temperature, amount of precipitation and soil water content, and the occurrence of the fungus Laetisaria fuciformis is influenced by the fertilisation scheme, as the % infection with the fungus was highest when organic fertilisers with low % nitrogen was used. The selection of fertilisers is seen as an important factor for the turfgrass colour.

Corrigendum in: CJFS. 2023 Feb 27; 41(1):78. doi:10.17221/26/2023-CJFS

Growing consumer awareness is forcing food producers to supply raw material and products of increasingly high quality and health-promoting properties. Knowledge of the genetic background of quality characteristics is taking on great importance, enabling selection based on molecular markers. The increasing throughput of molecular techniques, in combination with an expanding bioinformatics infrastructure, is leading to continual improvement in understanding of the molecular mechanisms influencing meat quality. This has resulted in the identification of polymorphic nucleotides [single nucleotide polymorphisms (SNPs)] showing a relationship with meat characteristics such as tenderness [polymorphism in the calpain (CAPN) and calpastatin (CAST) genes], marbling [diacylglycerol o-acyltransferase 1 (DGAT1)], colour, pH and water-holding capacity (WHC) [CAST, stearoyl-CoA desaturase (SCD) and others], and fatty acid profile (SCD1). An increasingly wide range of methods is used for analysis, from techniques based on amplification of nucleic acids [polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system-PCR (ARMS-PCR)] through Sanger sequencing to high-throughput next-generation sequencing (NGS) techniques. This paper is a review of the literature on polymorphism of genes determining the quality characteristics of meat and molecular methods used to detect them.

Soil rill erodibility (K_r) and critical shear stress (τ_c) are important parameters in some physical soil erosion models. In the present study, the temporal variations in K_r and τ_c by overland flow were investigated using undisturbed topsoil samples collected from three cropped plots (ryegrass, lucerne, and corn) and a fallow plot. In this study, the potential factors leading to these changes were examined from March to September 2022 in western Sichuan Province, China. The results revealed significant seasonal variations in K_r, τ_c, soil bulk density (SD), soil cohesion (SC), and soil water-stable aggregate (WSA). The K_r values were significantly lower, whereas τ_c values were slightly higher for cropped plots when compared to that for the fallow plot. The mean K_r values for cropped plots were 4.51~17.26 times lower than that for the fallow plot. In contrast, the mean τ_c values for the cropped plots were 1.03~1.08 times higher than that for the fallow plots. The results also indicated a negative correlation of K_r with SD, SC, WSA, soil organic matter content (SOC), and root weight density (RWD), while a positive correlation of τ_c with SD, SC, WSA, and RWD. Furthermore, an exponential decrease in K_r was observed with an increase in SD, SC, WSA, SOC, and RWD. An increase in SD, SC, WSA, and RWD of the experimental plots led to a further increase in τ_c.

Heat stroke is a clinical emergency secondary to a severe temperature increase due to the inefficiencies of heat dissipation mechanisms, causing central nervous system dysfunctions, which may lead to multiple organ dysfunction syndrome. Heat disturbances can be classified as "classic" or "exertional" ones and several predisposing factors are involved in their development. This review provides a broad approach to the pathophysiological mechanisms of this syndrome, and the diagnostic and treatment methods to facilitate their approach in clinical routine and increase the survival rate of patients.

The exact determination of the rotation period is still a current and important essential issue of forestry. It attracts the attention of forest economists, managers and owners worldwide, not only of forest economists but also of forest managers. The rotation period is defined by physical, technical or financial parameters of forest management. Therefore, it is necessary to distinguish between the biological and the economic optimal rotation period. A fundamental challenge in forest management is the need for appropriate determination of the rotation period. The primary interest of our research was to compare the effective legislation for the determination of the rotation period in the Czech Republic and in the Slovak Republic. Scientific methods such as document analysis and questionnaire survey were applied. The results of the legislation analysis and other related documents were compared with the expert opinions of the relevant stakeholders. Those who affect the decision process related to the problem and those where are "affected" by the problem were involved in the study. Results show that respondents do not agree with the regulation of rotation period according to effective law. Moreover, they consider it as not usable as the conditions in forest ecosystems have changed recently, which is not considered in the legislation.

Sulfonamides are widely used in treatment of animals and humans but pose a risk as environmental pollutants. An analysis of 1588 publications focused on sulfonamides is presented here. The review deals with environmental pollution with sulfonamides, described in papers indexed in the database Web of Science from 1938 to 2011. More in depth details are presented regarding publication activity during the last ten years in which 1255 papers have been published by authors from 1100 institutions. Papers, published during the last three years and mainly in 2011, are listed in comprehensive tables, sorted according to five criteria: reviews, contaminated niches, risk of contamination, sulfonamide transformation and methods of analysis. Key words and shortened abstracts direct the reader to the topics of interest. Hyperlinks to full papers, published in open access journals, are another aid in knowledge dissemination. This design of the review article allows easy navigation through vast amounts of information. Finally, a case report illustrates experiences from the author's laboratory with sulfamethazine determination in pig slurry by ELISA. The reported results highlight the need for updating the legal directives for environmental protection.

This study aimed to characterise the specific phenotypic responses and the sensitivity of photosynthetic parameters to progressive drought in modern wheat genotypes. In pot experiments, we tested eight wheat genotypes (Triticum sp.) that differed in ploidy level and country of origin. Water stress was simulated by the restriction of irrigation, which led to a decreased leaf relative water content of up to 70%. During gradual dehydration, changes in the structure and function of photosystem II (PSII) were analysed using the fluorescence parameters derived from fast fluorescence kinetics (OJIP transient). The results indicated that a group of JIP test-based parameters demonstrated sensitivity to drought, including genotype-specific responses. Severe drought stress led to a decrease in the photochemical efficiency of PSII (Fv/Fm), a reduction in the number of active PSII reaction centers (RC/ABS) and a decrease in parameters, indicating overall photochemical performance at the PSII level (performance indices PIabs and PItot). These findings demonstrate that the approaches used in our experiments were useful and reliable in monitoring the physiological responses of individual varieties of wheat exposed to stress conditions, and they have application potential as selection criteria in crop breeding. The contribution of the high-temperature effects on the photochemical responses under water deficit conditions is also discussed.