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The measures of Common Agricultural Policy (CAP) and Regional (Cohesion) Policy are aimed at achieving cohesion, i.e. reducing the economic (sectoral), social and territorial disparities at European Union level. The issue of rural areas has become an important part of the CAP measures in Pillar 2. Since the CAP does not have a primarily territorial orientation, it does not contribute to territorial cohesion of rural areas. In a similar vein, regional policy, which is primarily oriented towards territorial development, also failed to give rise to complete, balanced development. Using qualitative analysis, this article summarises references selected on the basis of the authors' experience in policy issues. The types of discourse included on the subject of coherence of common policies are the role of agricultural, rural and regional policy in cohesion and the conflict of cohesion and competitiveness objectives. Modern political and economic efforts of converging competitiveness into the macro concept with spatial implication result in planning and implementation of inappropriate measures and instruments intended to encourage the balanced territorial development of the EU. From this arises a conflict of cohesion and competitiveness, coupled with long-term consequences for the most vulnerable areas.

The paper pertains to the issue of quality assurance methods. Particular emphasis is placed upon the examination of hazards and possible product defects in food processing. At present, Polish regulations oblige any food processing entity to implement HACCP system. The system is perceived as a universal vaccine preventing the emergence of possible hazards or reducing the risk to an acceptable level. As far as defects are concerned, these are either disregarded altogether or do not occur at all. Meanwhile, the practice proves the case is not so. The research aspect of the paper revolves around an attempt to transplant solutions primarily applied in the automotive sector onto food processing industry. The practical aspect of the paper is manifested in the analysis of causes behind defects and hazards conducted on the basis of bakery industry. Potential hazards and product defects emerging from the process were identified in the course of the study. In addition, in order to eliminate these, their level was established with the application of various methods.

Knowing the time of death of animals can be helpful for the forensic determination of death due to cruelty. We aimed to determine the time of death of companion animal carcasses, euthanised at an outpatient's department (Small Animal Clinic) of the University of Veterinary Medicine and Pharmacy in Kosice. The reasons for euthanasia included age of animals, medical state or incurable disease which affected the quality of life. Animal carcasses (33 bodies) were divided into seven groups according to their weight, species and environmental conditions into which they were placed, which were chosen so as to imitate real conditions under which dead animals could be found. We continually measured body temperature until it dropped down to ambient temperature. The post-mortem cooling curve revealed dependencies related to the temperature drop, the weight of carcasses, the place where animals rested and the internal and external environment. Results from the cooling process and obtained time of death may be deduced from a nomogram in field practice.

The poor state of health and increased mortality rate of young roe deer, as reported by South Moravian hunters, caused by the increasing numbers of adult nematodes in the lungs of roe deer prompted us to identify the parasites using a combination of morphological measurements and a phylogenetic SSU rRNA analysis. The study was conducted in a 294 ha game reserve in South Moravia, Czech Republic. Molecular and morphometric techniques were used to identify adult nematodes collected from the respiratory tracts of nine 4-5 months old roe deer in poor health (low body weight of 3-4 kg, poor haircoat quality, and, in some cases, symptoms of diarrhoea). The morphological identification was based on a combination of adult worm characteristics corresponding to Dictyocaulus capreolus. A small subunit rRNA (SSU) partial sequence analysis showed the highest identity scores (99%) corresponding to the sequences of D. capreolus from a roe deer (GenBank: AY168859) from Sweden and the outcomes of the phylogenetic analyses resulted in a tree with a high branch support for two groups, with our sequences forming a well-supported clade with D. capreolus and Dictyocaulus sp. ex Capreolus capreolus (FJ589016) and Dictyocaulus sp. ex Rupicapra rupicapra (FJ589019) sequences from Spain. The examined roe deer have shown symptoms of diarrhoea, anorexia, and respiratory tract inflammation indicating that there might be a connection to the clinical importance of the Dictyocaulus infection.

The aim of the study was to evaluate the amount of nitrogen taken up from air, mineral fertilizers and soil by yellow lupine (Lupinus luteus L.) and spring triticale (Triticosecale Wittm. ex A. Camus) in their intercropping cultivation. The factor examined in the experiment was percentage of yellow lupine seeds and spring triticale grain in sown mixtures: 100/0, 75/25, 50/50, 25/75 and 0/100, respectively. Yellow lupine yield was reduced by spring triticale when intercropped. In the yield of both plants the percentage share of spring triticale grain was larger and that of yellow lupine seed was smaller than in the sown mixture. Land equivalent ratio informs that yield advantage under intercropping of lupine and triticale was minor. The total protein yield was the highest in lupine cultivation when sown pure, and decreased with increasing share of triticale in their intercropping. The mean percentage nitrogen share from N₂ fixation, fertilizer and soil reserves in yellow lupine mass was: 65.2, 8.8 and 26.0%, respectively, while in the mass of spring triticale it was 10.1, 23.5 and 68.8%, respectively.

In this study, a global agricultural trade network was constructed, and its topological characteristics from 1991 to 2021 were analysed. The influences of nine variables were empirically tested, including factor endowments and economic, geographical, and institutional factors. The research results show that the scale of trade networks is constantly expanding, and trade relations are becoming increasingly concentrated. Further, global agricultural trade patterns are gradually being reshaped. However, European economies and the United States still dominate the power of network control, with a clear ‘core-edge’ hierarchy. Among the factors influencing the global agricultural trade network, differences in arable land areas, agricultural product prices, geographical distances, and financial institutions have proven important. However, their influence varies. Compared to the differences in the endowments of other factors, the comparative advantage of agricultural trade in various nodes worldwide comes more from the arable land areas. The greater the difference in agricultural prices, the closer are the trade ties between nodes. Differences in geographical distance have proven conducive to establishing agricultural trade relations. Finally, the greater the difference in financial systems, the greater the likelihood that agricultural trade links will occur.

Hydrocarbon contaminants have become a global concern due to their long-term adverse effects on soil ecosystems and human health. Successful implementation of phytoremediation to clean up hydrocarbon contaminants requires the identification of the most effective remediation plant species. Twelve native plant species of the Sudd Wetland in South Sudan were evaluated for their potential application as phytoremediators. The treatments included six total petroleum hydrocarbon (TPH) concentrations of 0, 25, 50, 75, 100 and 125 g/kg soil. The twelve native plant species tested were: Sorghum arundinaceum Desv., Oryza longistaminata A. Chev. & Roehrich, Hyparrhenia rufa Nees, Abelmoschus ficulneus L., Gossypium barbadense L., Nicotiana tabacum L., Sorghum bicolour L. Moench, Eleusine coracana Gaertn., Capsicum frutescens L., Zea mays L., Tithonia diversifolia Hemsl. and Medicago sativa L. Significant differences in phytoremediation rates were observed amongst the treatments with exception of the 125 g/kg soil concentration of hydrocarbon that was lethal to all the plant species. Over 50% TPH reduction in the 75 g/kg soil concentration was observed in contaminated soil phytoremediation in H. rufa, G. barbadense, O. longistaminata, T. diversifolia and S. arundinaceum, making them potential phytoremediators of hydrocarbon-contaminated soil in the Sudd-Wetland of South-Sudan.

Grape hyacinths are popular perennial, flowering bulbous plants. In 2018, 2019, and 2020, some plants of three Muscari species showed symptoms similar to those associated with phytoplasma infection in commercial ornamental gardens in Hungary. Symptoms included virescence of flowers and yellowing of leaves. Symptomatic and asymptomatic Muscari plants were sampled at two locations to detect and identify the pathogens involved. Total DNA was extracted from the plants and used as a template in polymerase chain reaction assays to amplify 16S rRNA gene sequences and housekeeping genes (tuf, secY) with phytoplasma-specific primers. The resulting PCR products from symptomatic plants were cloned and sequenced bidirectionally. Homology searching of the obtained sequences against the GenBank database indicated the presence of  'Candidatus Phytoplasma asteris' in the three Muscari species. This is the first report worldwide of C. P. asteris phytoplasma infecting M. botryoides and M. comosum, and its first identification in M. armeniacum in Hungary.

The comprehensive risk analysis of a business such as farming entails questions on what is at stake, how important is the risk concern and how to deal with it. We performed a sequential mixed method, with the in-depth interviews in the first stage (n = 35), followed by a survey on the Flemish FADN (n = 614) in the second, to investigate the farmers' risk perception, the attitudes towards risk and the perceived usefulness of the risk management strategies. We find that, rather than the short-term volatility in prices, the longer term co-evolution of expenses versus receipts is of a major concern to farmers, next to the land availability and the policy risks. Farmers are shown to be only slightly risk averse, rather risk neutral even. Further, our results suggest that farmers do not consider extensively studied risk management strategies such as contracts, futures and insurances, a valid option for their farm, and put more faith in internal strategies such as the debt management, the liquidity management and diversification. Last, risk management is to a substantial degree performed at the household level, rather than at the farm level, with strategies such as cutting the private expenses and the off-farm employment. These results hardly differ according to the farm and farmer characteristics.

One of the most significant environmental problems in Europe is the land use change as a result of urbanization. The estimate of future agricultural land takes in the Czech Republic previously published in this journal is alarming; however, this is based on arbitrarily determined assumptions. Our contribution brings a more realistic assessment of the extent of expected land takes (example of the Hradec Králové Region). For this purpose, the data from the municipalities' Planning Analytical Materials (PAM) on buildable areas (and redevelopment areas) and data on the existing expansion of built-up areas are used. Particular attention is paid to the best quality soils included in the 1^st and 2^nd protection class of agricultural land resources (ALR), because some municipalities located in fertile agricultural areas argue about the necessity to build up good-quality land. The Pearson correlation coefficient has been used for the evaluation to what extent the share of the soils included in the 1^st and 2^nd protection classes of ALR out of the total area of the municipality is really related to the share of best quality soils in planned buildable areas. The spatial statistics method ‒ geographically weighted regression (GWR) has been used to find spatial deviations from the global relationship model. There is a clear differentiation between the municipalities as to whether they are able to rather protect the best soil or whether they are planning future construction predominantly on it. E.g. in municipalities with about 30-50% of the land included in the 1^st and 2^nd ALR protection classes, buildable and redevelopment areas are designed from 0 to 100% for these highest classes of ALR protection. However, the total strength of the association (Pearson's r) between these indicators is large, r = 0.80 (or r = 0.95 when "the point-index value of agricultural land" was used instead of ALR protection classes). The results of GWR show that higher deviations from the model value, both positive and negative ones, are not spatially clustered but located next to each other. Greater deviations occur more frequently in the more fertile western part of the region, where there is a higher pressure on good-quality land, which is either intended for development or protected on the basis of local factors (including spatial planning of individual municipalities). Estimation of future developments has revealed a substantial over-dimensionality of planned buildable areas - they will potentially be built up in more than 100 years.

The aim of the submitted work was to analyze the outlook of logging perspectives in the next two decades (2013-2032) for all forests in the territory of the Czech Republic. The analyses made use of the national database of forest management plans and guidelines. For the purpose of these analyses, the forests were divided into commercially exploitable forests and forests commercially exploitable with restrictions. The latter ones included protection forests and the category of special purpose forests, i.e. 31c - forests in the territory of national parks and national nature reserves, and 32a - forests occurring in the first zones of protected landscape areas, and forests occurring in nature preserves and nature monuments. In addition to the potential total volume of timber logging, perspectives were studied separately for coniferous and broadleaved tree species as well as for individual main tree species. The derived model mean annual cut of 15.51 mil. m³ corresponds to the current timber production in the Czech Republic. Results of the analyses indicate that reduced logging of coniferous, namely spruce, timber is to be expected in the next twenty years.

A pot experiment focused on the study of factors influencing thallium transfer from contaminated soils into kale (green cabbage, Brassica oleracea L. var. acephala, variety Winterbor F1) was evaluated. Three different types of topsoils with naturally low content of thallium (heavy, medium and medium-light soil) were used for pot experiments. The soils were contaminated with thallium sulfate to achieve five levels of contamination (0, 0.52, 2.10, 4.20 and 5.88 mg/kg). There were six replicates for each combination (90 pots in the experiment). The first part of the experiment started in the year of contamination (2001) and continued in 2003. The soil samples and the samples of kale (leaves and stalks were sampled separately) were collected and analysed. Kale was found to be able to accumulate Tl without any influence on yield. The highest thallium concentration was found in the leaves of kale in the first year of the experiment and reached 326 mg/kg dry matter. Bioaccumulation factor (Biological Absorption Coefficient - BAC) was found to be over 80 during the first year of the experiment. In the third year the BAC was around 3 for the soil with the highest pH and the highest organic matter content but as high as 15 for an acid soil with the lowest content of organic matter and the lowest Cation Exchange Capacity (CEC) of soils. The content of thallium in the leaves of kale was found to be 7 to 10 times higher than in the stalks in the third year. In the first year this ratio was up to 18. From these findings it can be concluded that the ability of some plants of Brassicacea family, that are planted as common vegetables, to accumulate thallium is very high and can be a serious danger for food chains. Neutral soils high in CEC and organic matter are able to bind thallium more effectively than poor acid soils and the transfer of Tl into plants from these soils is substantially lower. The uptake of Tl from contaminated soils into kale can be very high and without any negative effect on the plant growth. The transfer of Tl into kale decreases with the time necessary to reach the equilibrium between the added Tl and the soil (ageing of a sample).

During 4 years, 27 cultivars and breeding lines of winter wheat (Triticum aestivum) were tested in small plot experiments for resistance to powdery mildew fungus. The most resistant were Frimegu, RE9607, Runal, Asset, Folke and Wasmo. The cultivars Asta (Pm2,6) and Vlasta (Pm2,6 and another not determined specific gene or minor genes of resistance) fall into resistant cultivars. It seems that the specific genes of resistance Pm2 and Pm6 are still very effective against the present Czech population of powdery mildew on wheat. Resistance of the cultivars Hereward and Tarso, having the gene of resistance Pm8, can be ascribed to an additional undetermined gene that is effective only in mature plants. The cultivars Mikon and Ramiro with partial resistance had a higher infection type and disease severity than resistant cultivars, but lower disease severity than the susceptible cultivar Kanzler.

In 2003 to 2005, Chrysomela populi L. gradated on yearly cut Populus nigra var. italica in street alleys in Brno and elsewhere. Imagoes occurred on trees from the end of April or from the beginning of May. In rearing, the chrysomelid consumed unwillingly leaves of 2-year shoots and laid on average 142 eggs. On leaves of shoots, imagoes damaged 100 to 200 cm² during 5 to 7 weeks, produced 480 to 900 frass pellets and laid on average 506 eggs. Oviposition was continual with breaks amounting to on average 2.6 days. Embryonal development took 6 to 8 (in the laboratory 5) days. Larvae of the 1^st generation occurred from mid-May to mid-July. In the course of 2 weeks (in the laboratory during 10 days) of life, they damaged about 20 cm² leaves and produced about 300 frass pallets. In the laboratory, prepupae took 2 days and pupae 4 days. Imagoes of the 1^st generation occurred from June to September. They damaged on average 113 cm² and produced on average 553 frass pellets and 653 eggs. At a temperature of 24 to 28°C, imagoes damaged on average 84 cm² during 2 to 3 weeks and diapaused until the next year. On growing up leaves, imagoes lived longer showing higher consumption of food and higher fecundity as against new fully-grown leaves. Larvae of the 2^nd generation destroyed on average 2 cm² smaller area than larvae of the 1^st generation. Part or all imagoes of the 2^nd generation diapaused. Imagoes of the 3^rd generation damaged 40 to 70 cm² leaves before departure to wintering grounds. In our natural conditions, the prospective 3^rd generation is always incomplete. Cleonice callida Meig. and Schizonotus sieboldi (Ratz.) rank among important enemies.

The bionomics of Acarus siro was studied at 18 and 20°C and 70 and 80% R.H. on wheat germs, linseed, rapeseed and sunflowers to obtain data for optimisation of biological control of stored product mites on oilseeds. Wheat germs were used as a control substrate as they were considered optimal for development of A. siro. The development of eggs and larvae was longer than that of protonymphs and tritonymphs. Wheat germs appeared to be a better substrate for the development of mites than oilseeds. Generally, the life span of females as well as the oviposition period was shortest on wheat germs while longest on sunflower; also, at the higher temperature the female life span was shorter. The maximum number of eggs laid by a single female (356) was recorded on wheat germs at 20°C and 80% R.H. The development was significantly shorter at 20°C than at 18°C regardless of humidity. There were no significant differences between the effects of the tested oilseeds.

The relationship between type traits in Holstein cows over generations was analysed. The analysed data set included a total of 12 157 dam-daughter pairs with linear scores of 14 type traits including a final score and general characteristics of type. Significant relationships existed between linear type traits in dams and their daughters. Pearson correlation coefficients ranged from r = 0.20 to r = 0.63 except the rump angle. A negative correlation (r = -0.02) was found for this trait. A similar tendency resulted from the analysis of the relationship between values of general characteristics and the final type score.

Two spring phenophases (bud-burst and leaf unfolding) of a parent stand and naturally regenerated undergrowth of European beech were observed in conditions under different stand density over the last 10 years. The results proved the unequal onset of phenophases of the parent stand individuals in relation to their sociological status. In the case of codominant and dominant trees the delay of 2-5 days was observed in comparison with subdominant trees. The influence of the parent stand structure on the onset of the undergrowth phenophases was also observed. The onset of phenophases differed by 2-20 days among individuals grown under different density of the parent stand. The onset and course of phenophases also differed between the years. The trend of the average onset of leafing in the period 1995-2004 shows a shift to earlier dates by about three days. Temperature summation of average daily temperatures with the base temperature of 8°C, in the framework of the model predicted bud-burst of beech, showed the lowest variability in comparison with other temperatures.

The aim of the present study was to develop a rapid and inexpensive method for the determination of methylmercury in fish tissues based on GC/ECD instrumentation. The new method is based on acidic digestion in hydrochloric acid and subsequent extraction with toluene. Methylmercury is determined by the GC/ECD technique using a DB-608 capillary column. The following parameters of the method were established: detection limit 13 μg/kg, limit of quantification 22 μg/kg, linearity 0.2-200 ng/ml, reproducibility 9.4%, and recovery 90%. The method was developed and verified using CRM 464 reference material and was successfully tested in inter-laboratory comparisons IMEP - 20 "Trace elements in tuna fish" organised by the Joint Research Centre - Institute for Reference Materials and Measurements (Belgium), with the success rate of E_n=0.43.

A modified electrometric method was described in sheep, goats and cattle and used to demonstrate in vitro inhibition of plasma and erythrocyte cholinesterase activities by the organophosphate and carbamate insecticides dichlorvos and carbaryl, respectively. A typical reaction mixture for the measurement of cholinesterase activity contained 3 ml distilled water, 3 ml barbital-phosphate buffer (pH 8.1), 0.2 ml plasma or erythrocytes and 0.1 ml acetylthiocholine iodide (7.5%) as a substrate. The mixture was incubated at 37^οC for 30 min in sheep, 40 min in goats and 20 min in cattle. The pH of the reaction mixture was determined by a pH meter before and after the incubation. The initial pH was measured before the substrate addition. The enzyme activity was expressed as ∆pH/incubation time = (pH1 - pH2) - ∆pH of blank. The method of inhibitor-cholinesterase incubation was used to measure the in vitro inhibition of plasma and erythrocyte cholinesterase activities. Dichlorvos in concentrations of 0.5 and 1 μm inhibited plasma and erythrocyte cholinesterase activities by 24-85%, whereas carbaryl in concentrations of 5 and 10 μm inhibited them by 50-89%. The results suggest that the described electrometric method could be efficiently used for detecting cholinesterase inhibition in ruminants, and further point to the value of the present experimental protocol of in vitro cholinesterase inhibition in preliminary toxicological examinations of anticholinesterase compounds

The purpose of the present study was to investigate the effects of feeding peat as a supplement in the period after weaning on the performance and health status of pigs. Also to assess the risk of the development of tuberculous lesions in the lymph nodes and parenchymatous organs, caused by conditionally pathogenic mycobacteria present in peat. Twenty Large White × Landrace pigs in equal numbers of barrows and gilts (mean live weight 18.0 ± 1.7 kg) were used in the experiment. The experimental group was fed a diet containing commercial underground peat in the dose of 80 g peat/kg dry matter for 30 days. Subsequently, they were fed an identical diet with the control group without peat for 60 days. A short-time feeding peat did not significantly affect the growth and performance of pigs. From day 21, a statistically highly significant (P < 0.01) increase in the consumption of the experimental diet was recorded, however, without a positive effect on the growth of experimental animals. The conversion of the peat containing diet was comparable to the conversion of the control diet. It follows from the results of biochemical analysis of blood that peat feeding for 30 days did not adversely affect the metabolic profile and health status of experimental animals. No tuberculous or tuberculoid lesions in lymph nodes or parenchymatous organs were detected in any of 20 slaughtered animals. Despite that, mycobacteria were isolated from 10 (25.0%) tissues of 5 (50.0%) pigs from the experimental group. One isolate was identified as Mycobacterium avium subsp. hominissuis (IS901- and IS1245+). Seven of nine isolates were determined as conditionally pathogenic atypical mycobacteria: M. fortuitum (n = 2) and M. xenopi (n = 5). It follows from the present results that feeding of a peat supplemented diet to pigs may be considered as economically non-effective and due to the findings of mycobacteria as risky.

Fungi representing Alternaria spp. are ubiquitous pathogens that may under certain conditions cause spoilage of various food crops. Several Alternaria species are known producers of toxic secondary metabolites in some fruits and vegetables, nevertheless, only limited knowledge is available on the occurrence of these mycotoxins in legumes and/or oilseeds used for human nutrition. In the first part of the presented study, the analytical method employing reversed phase high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD) was implemented to enable the examination of these food commodities for the presence of altenuene (AE), alternariol (AOH), and alternariol monomethyl ether (AME); the limits of detection were 1, 3 and 2 µg/kg for AE, AOH, and AME, respectively. Altogether 122 flax and 84 pea seed samples grown under organic and/or conventional farming conditions were analysed in the years 2002-2003. AME was detected in 20 flax seed samples; AE and AOH were present in only 2 and 4 samples, respectively. More frequent incidence of Alternaria toxins was recognised in fibre flax seeds as compared to linseed samples. Compared to the crops from the conventional farming, the concentrations of these mycotoxins found in positive organic samples were higher. No Alternaria mycotoxins were detected in the pea samples, probably due to the presence of antifungal compounds in the respective crop.

Presented paper is dealing with operation load of three-point hitch (TPH) of agriculture tractor in randomly changeable working conditions and their simulation in laboratory. The basic goal was to obtain experimental data, after which we can judge and analyze problematic of dynamic load of wheel tractor at plowing with orientation on TPH. Another goal was using obtained result from operating measures on their simulation in laboratory conditions by hydrostatic simulator. In presented paper is solved power analysis in each elements TPH of agricultural tractor ZTS 160 45URII A. The tractor during exploiting was in set with four-ploughshare carried and five-ploughshare semi-mounted plough. The measuring was realized for 3 varieties of set speed (gear ratio I/2, I/3, I/1), 3 depths of plowing (20, 25 and 27 cm) and 3 varieties of TPH - positional, mixed and draught control.

In 2002 a study focused on the evaluation of height and density of expanding natural regeneration of Fraxinus excelsior L. (FE) was carried out on Velká hora Hill, a locality in the Bohemian Karst (Český kras). The examined area is located in Karlštejn National Nature Reserve and takes up around 31 ha. The parent rock is limestone. The expansion depends on soil and exposition conditions and relates to the water balance gradient. FE reached the highest densities (up to 6,000 individuals/400 m²) on Velká hora's peak plateau on medium-deep, heavy-textured decarbonized soils. The lowest expansion (508 individuals/400 m²) was found on slopes fully exposed to south (S) with water retention capacity lower than 20 mm. In total, the average density was 1,190 individuals/400 m². FE natural regeneration reached the highest average heights (around 210 cm) on Fageto-Quercetum illimerosum mesotrophicum, the smallest heights on Carpineto-Aceretum saxatile. Average height in the locality was 47 cm. No relation was found between FE natural regeneration height or density and the distance of a fertile specimen. The distance exceeded 70 m only in 3.4%. FE seeds could be detected almost everywhere at the area. FE is capable to establish itself on any location of the studied locality except ecologically extreme parts of rock steppe without forest and Fagus sylvatica L. stands occupying S slopes. On less favourable sites it is capable to use the protection of other tree species and as a low growing tree it can eventually dominate the site. In more favourable conditions it expands on the whole area, where it can dominate the undergrowth already at 1-m height. If the forest sites are left to natural development, a distinctive change in the tree species composition could take place in a short time period. Such a change could have an essential impact on light conditions, energy flux and species composition of plant and animal communities.

Disulphide bonds and sulphhydryl concentrations were evaluated to determine the effects on rheological, thermodynamic, pasting, and dynamic rheological characteristics of mixed flours. Gluten samples, first treated with sodium sulphite of different concentrations, were added into flour at a 4% level, which had a significant impact on free sulphhydryl, disulphide bonds, and the ratio of the two indices. There was no relevance between the ratio and other parameters except for free sulphhydryl. The mixed flour doughs had reduced water absorption, dough development time, dough stability time as well as degree of weakening (P < 0.05). Disulphide bonds were associated negatively with the rate of starch gelatinisation (C3-C2), peak, and setback and these characteristics were correlated strongly with dough development time, dough stability time, and progressive protein weakening (C2-C1). The stability of starch gelatinisation and cooking stability of mixed flours did not remain significantly different. The larger the concentration of sodium sulphite, the higher the peak, breakdown, final viscosity, and setback values, but there were no significant differences between samples. For all samples, storage modulus and loss modulus increased with increasing scanning frequency. For mixed doughs, the trend lines of moduli decreased with increasing levels of reduction in added gluten. There was no substantial effect on thermal properties of flours.

The interaction of manganese with cadmium (Cd) toxicity was studied on maize plants grown in hydroponics. Manganese supplied as MnSO₄clearly alleviated the toxic effect of cadmium on the root growth of maize seedlings. The magnitude of alleviation was dose dependant and total abolition of 10µM Cd toxicity on root growth was observed at Mn/Cd ratio of 20:1. The 12 h pre-treatment with 10μM Cd was generally toxic for nitrate uptake and reduction (both determined in Cd-free media). The beneficial effect of 100μM Mn on this toxicity was confirmed for the low-affinity nitrate uptake system, but on the other hand, Mn alone seems to be slightly toxic for high affinity nitrate uptake system and on the nitrate reductase activity.

Forty male lambs of the mountain Greek breed were used to evaluate the effect of age at slaughter on carcass characteristics and composition. The slaughter of lambs was carried out at the age of 30, 45, 60, 75 and 90 days. The slaughter procedure, carcass dissection and carcass composition were realized according to the standard method of CIHEAM-AGRIMED programme. The results of this work showed that the lambs of different age groups did not differ in dressing percentage significantly. The proportion of muscles showed a tendency of increase with the increasing slaughter age but the differences were not significant. The proportion of total fat in carcass increased with the increasing slaughter age from 20.84 to 23.59% for the age group of 30 and 90 days, respectively (P < 0.05). On the contrary, as the age at slaughter increased, the proportion of bones decreased (P < 0.05). Subcutaneous, intermuscular, perinephric and pelvic fats increased with the slaughter age, but only the subcutaneous fat was influenced significantly (P < 0.05). Among the different commercial cuts proportions of long leg, first 5 ribs and shoulder decreased, while those of last 8 ribs-loin and breast-flank increased (P < 0.05). The overall results suggest that the slaughter age of lambs of the mountain Greek breed should be increased from the present ~45 days to more advanced age of 75 or 90 days in order to improve the farmers' profits without serious negative effects on the quality of carcasses or meat of lambs.

An expert estimate of the weight of emissions produced in agriculture has been up to now made only through a final counting to the total REZZO 4 emission balance in the category of "other mobile sources" The existing situation is however unbearable since a proper methodology to determine the production of emissions in agriculture, i.e. in the department with a considerable consumption of fossil fuels, is still missing. The solution consists in a more precise specification of the weight of generated limited pollutants (CO, NO_x, SO₂, PM and VOC including CO₂) in the department of agriculture on the basis of the measured annual consumption of fuels in agriculture and with using the emission factors of fuels. Calculated results are compared with the original values finally counted for the REZZO 4 category of "other mobile sources" in 2000 and 2001 (Adamec 2002; Adamec et el. 2003). The calculation revealed that the weight production of individual pollutants in 2000 and 2001 reached only 28% and 27% for CO, 52% and 50% for NO_x, 69% and 66% for SO₂, 87% and 83% for PM, and 26% and 24% for VOC of the original estimates with final counts and was therefore much lower. The share of agriculture in the weight production of emissions from mobile sources in 2000 and 2001 was 3.1% and 3.1% for CO, 11.5% and 11.5% for NO_x, 19.8% and 18.8% for SO₂, 38.3% and 34.6% for PM, and 3.5% and 3.6% for VOC. The development of weight production for individual pollutants in the period from 1995-2005 is expressed by means of regression equations. Coefficients of reliability indicate that the measure of reliability of the interval determined by calculation is much higher than that of the reliability interval determined by values estimated through final counting that appear incidental. There are increasing efforts today focused on the replacement of diesel oil as a traditional fossil fuel in agriculture with biodiesel oil as a more environment-friendly fuel. The second part of results includes a monitoring of the impact of biodiesel oil emissions in cases where diesel oil was replaced by this ecological fuel in agriculture in the period from 2000-2005. It follows from the analysis that the weight production of pollutants in 2000-2005 would have been reduced by 4% in CO, by 28% in SO₂, by 52% in PM and by 4% in VOC while an increase by 20% and 32% would have been recorded in CO₂ and NO_x, respectively. Regression equations are used to express the development of the weight production of individual diesel oil and biodiesel oil pollutants in the period from 2000-2005. Reliability coefficients that are of constant character indicate that the development of the weight of pollutants from diesel oil replicates the development of biodiesel oil pollutants. The significance of achieved results consists in the provision of a more accurate general balance of emissions from one of so called other mobile sources in Czech Republic (apart from the department of transport), thus contributing among other things to a more accurate expression of the total weight of emission production within REZZO 4.

The formation of 3-chloropropane-1,2-diol (3-MCPD) released from its esters with higher fatty acids was studied using the recognised precursors of 3-MCPD (tripalmitin, 1,3-dipalmitin, 1-monopalmitin and soybean oil) in the presence of sodium chloride. The precursors were reacted with sodium chloride in an emulsion stabilised with an emulsifier under conditions which modelled the thermal treatment of foods during processing. The highest amount of bound 3-MCPD (released from its esters) was formed from 1-monopalmitin followed by 1,3-dipalmitin, whereas tripalmitin and soybean oil yielded the lowest levels of bound 3-MCPD. Four sets of experiments were then carried out aimed at monitoring the influence of various factors (soybean oil amount, NaCl content, water content, and temperature) on the yield of bound 3-MCPD. The formation of bound 3-MCPD was directly proportional to the concentration of either oil or NaCl. The highest amount of bound 3-MCPD was formed in media containing approximately 20% water. The amount of bound 3-MCPD decreased with increasing temperature over the range 100-230°C and reached its highest value at 100°C. Models with 1,2-dipalmitoyl-3-chloropropane-1,2-diol showed that the decomposition rate of this ester rapidly increased with increasing temperature over the range 100-230°C being the lowest at 100°C and the highest at 230°C.

In 2003, 2004 and 2005 milk fat composition was determined three times in 55 dairy cows of Czech Pied cattle housed in a byre with stanchions and fed under conditions usual on Czech production farms. Fatty acids were determined by a gas chromatographic method, 26 acids out of the total 37 acids observed in chromatograms were identified. The highest proportions were observed for palmitic acid (29.25 ± 2.98%), oleic acid (24.47 ± 3.27%), myristic acid (12.14 ± 1.80%) and stearic acid (8.91 ± 2.44%). The proportions of saturated, unsaturated and monounsaturated fatty acids were 64.71 ± 4.18, 31.96 ± 4.20 and 27.45 ± 3.42% of total acids, respectively. The total proportion of nutritionally undesirable lauric, myristic and palmitic acid was 45.26 ± 4.77%, while that of the desirable group of polyunsaturated fatty acids was 4.51 ± 1.09%. The observed relatively wide ranges of the individual groups of fatty acids indicate that it is possible to improve the milk fat composition in Czech Pied cows.