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Autologous lamellar corneal grafts were performed on four dogs with two deep corneal ulcers and two corneal perforations to achieve better visual and aesthetic outcomes. The donor corneal graft was harvested from the relatively normal corneal region of the affected eye and used to cover the corneal defect. At the two-year follow-up examination, ultrasound biomicroscopy was performed to confirm the thickness and endothelial continuity of the transplanted grafts and donor site of the cornea. The evaluations revealed that the dogs had healed without incident and that their vision had been maintained. Furthermore, the owners were satisfied with the aesthetic outcomes in all cases. Our findings show that autologous lamellar corneal grafts are useful and effective in treating canine corneal ulcers and perforations.

The resistive index (RI) is an indirect measurement of arterial resistance by means of a ratio between the peak systolic and end diastolic velocities recorded with a spectral Doppler device, especially used to evaluate the vascular damage in ocular diseases such as glaucoma. Some ocular variables such as the intraocular pressure (IOP), the choroidal thickness, the axial length and the ocular blood flow may be influenced by physical exercise. The purpose of this study was to evaluate the influence of the exercise on the RI of the medial long posterior ciliary artery in dogs, and correlate the data obtained with the IOP values. Ten clinically healthy dogs were subjected to moderate physical exercise on a canine motorised treadmill at different speeds for 45 minutes. A colour Doppler examination was performed and the RI values were calculated for the medial long posterior ciliary artery at rest, immediately after the exercise, and after 60 minutes at the end of the exercise. At the same times, the IOP was recorded by applanation tonometry. The data were analysed by a two-way repeated ANOVA measurement in order to compare the RI and the IOP. Wilcoxon's test was applied for the post hoc comparison. Spearman's rank correlation for non-normal distribution was used to determine a relationship between the RI and the IOP. The at rest RI was 0.722 +/-0.022, IOP 12.38 +/3.21 mm Hg. A significant decrease in the RI was observed immediately after the exercise (0.697 +/-0.035) and during the passive recovery phase (0.682 +/-0.042). A significant decrease in the IOP (11+/3.39 mmHg) was recorded after 60 min of the passive recovery phase; at the end of the exercise, a slight decrease (12.29+/4.26 mm Hg) mm Hg was detected. During the test, a linear correlation between the RI and the IOP was observed. Our results suggest that exercise induces the modification of the ophthalmic blood flow in dogs, presumably related to the compensatory neuro-hormonal mechanisms.

The paper deals with Czech households' subjective views on food waste. It examines households' attitudes to food waste, including the causes of food waste. The primary data were obtained via a questionnaire survey with 1 582 respondents. The analyses allowed the identification of three categories of Czech households with different attitudes to food waste and its causes. These findings provide a basis for a proper selection of corrective measures aimed at amending or even completely transforming the current behaviour.

The decline of soil organic carbon (SOC) has aggravated salinity-related problems in semi-arid irrigation areas of the Awash river basin, Ethiopia. This study aimed at evaluating the performance of potential remediation crops on saline soil and their effectiveness for remediating soil salinity and improving pH, SOC, bulk density (BD) and hydraulic conductivity (HyCo). Rhodes grass (RHG), alfalfa (ALF), sudangrass (SUG) and blue panicgrass (Retz) (BPG) were grown in saline (3-13.9 dS/m) field plots. The crop biomass was incorporated into the soil immediately before flowering. The results show that at high soil salinity levels, BPG and SUG grew well, with the harvesting frequency of BPG being much higher than for SUG. Conversely, the growth of ALF and RHG was strongly inhibited by high soil salinity. Significant (P < 0.05) reduction of soil salinity levels (-3.2 dS/m) and related ionic concentrations, an increase of SOC (0.8% to 1.6%) and improvement of BD and HyCo were observed in BPG plots. The fast-growing nature of BPG in the hot climate of the experimental area resulted in harvests every three weeks and promoted the incorporation of high amounts of biomass to the soil and efficient soil salinity remediation. At moderately saline conditions, ALF also showed a great potential for salinity reclamation (-1.8 dS/m) and SOC accumulation. The cultivation of fast-growing annual crops proved an efficient and low-cost strategy for soil salinity mitigation and the reclamation of salinity-associated soil degradation in irrigation agriculture in Ethiopia.

The following updates have been proposed for the XYZ system for the production of hybrid wheat: The waxy characteristics of the grain were used as a classifying mark. The candidate pollen sterility gene TIP2 was detected in silico based on similarity to known pollen sterility genes in rice. In order to maintain a sterile maternal component, the addition chromosome 7H was proposed, carrying wild-type alleles Waxy-H and TIP2-H. The concept of practical production of the commercial F₁ seed was designed.

High-throughput sequencing (HTS) analysis of tomato (Solanum lycopersicum) samples revealed the presence of Potato virus M (PVM) in this crop in Slovakia. Full-length genomes of three PVM isolates were obtained using both HTS and Sanger sequencing validation. While two isolates (T40 and T50) were shown to belong to major Group I, a divergent T20 isolate was phylogenetically unrelated to any known PVM variant, potentially representing a new phylogenetic group. Despite a relatively high intraspecies diversity (17.3 ± 0.3%), no evidence of recombination was detected in the dataset of available complete PVM sequences. Conventional screening of tomato plants in Slovakia using ELISA and RT-PCR further confirmed a frequent occurrence of PVM in this host. Developed RT-PCR showed its polyvalence to detect the PVM Group I isolates, however, in silico analysis of primer binding sites indicated its compromised use for Group II isolates. Our results further pinpoint the significance of HTS for unbiased unveiling of virus diversity and a need for continual optimisation of molecular detection tools.

The present study aimed to evaluate the effect of two dietary Ca levels (3.00% vs 3.50%) in three hen genotypes (ISA Brown, Bovans Brown and Moravia BSL) on their laying performance and quality of their eggs. In order to evaluate the response to dietary Ca, an experiment was conducted with 300 laying hens. One hundred birds from each genotype were divided into 10 replicates (10 birds/cage/Ca level). To reveal the differences, the following characteristics were measured: hen-day egg production percentage, daily feed intake, egg weight, albumen and yolk weights, Haugh unit, eggshell colour, eggshell weight, eggshell thickness, eggshell strength, egg shape index and eggshell index. A higher Ca level significantly increased the hen-day egg production percentage of Bovans Brown and Moravia BSL, while ISA Brown laying hens had the higher egg production percentage at a lower dietary Ca level (85.97% vs 82.41%). No significant effect of dietary Ca level was observed on any internal egg quality measurements except the Haugh unit. However, the dietary Ca level significantly affected some of external egg quality measurements, namely eggshell colour (P < 0.001), eggshell weight (P = 0.003) and eggshell strength (P = 0.012). The results of the present study show that laying performance and egg quality parameters responded differently according to hen genotype and dietary Ca level. Bovans Brown and Moravia BSL laying hens required higher levels of Ca to keep egg production high, while the eggshell quality measurements were better at the lower dietary Ca level. Thus, it is recommended to adjust the Ca levels in feed to the nutritional requirements of laying hens.

Two Hanwoo steers were emergency-slaughtered due to symptoms of urethral obstruction. The steers were of the same age and were raised using the same feed management. The remaining 22 cattle in the finishing period showed no symptoms of urinary calculi until 30 months of age and were examined for the presence of bladder calculi when slaughtered. Bladder calculi were observed in 11 of 22 steers at slaughter (50%) and cystitis occurred in 13 of 22 steers (59.1%). There was a significant relationship between the presence of bladder calculi and the incidence of cystitis (P < 0.05).

European fruit lecanium, Parthenolecanium corni (Bouché, 1844) is among the leading worldwide pests of fruits such as plums, currants, jostaberry, grapevine and many others. This study is focused on testing the effectiveness of natural insecticide treatments compared with conventional insecticides on overwintering nymphs of the European fruit lecanium. In February of 2017, two experiments were conducted under laboratory conditions. The tested products were Boundary SW^® (plant extract), Konflic^® (plant extract), Ekol^® (canola oil) and a tank-mix of Ekol^® + Reldan 22^® (canola oil and chlorpyrifos-methyl) as a chemical standard. The most effective product tested was the chemical standard tank-mix of Ekol^® and Reldan 22^®, which was 100% effective in all experiments. High efficiency was also achieved with Ekol^® (canola oil) in high concentrations. We can summarize, that using canola oil in high concentration has almost the same effect as the chemical standard. Using canola oil against European fruit lecanium meets the principles of Integrated Pest Management (IPM) and also the need to design cropping systems that are less dependent on synthetic pesticides.

The study analyses the correlations among different economies of selected EU-12 member states based on comparison of agricultural economics variances, namely the output value of the agricultural industry, productivity of input, agricultural gross value added, subsidies on production, agricultural labour input and agricultural income per annual working unit in the period of 2010-2016, based on the Special Program for Social Sciences, as statistical methods. The EU-12 achieved a higher increase in productivity of input, output value of agricultural industry, agricultural gross valued added, as well as agricultural income per agricultural annual working unit compared to the average results of EU-28 for 2010-2016. The output value of agricultural industry and agricultural gross value added per intermediate consumption decreased by 1.35% and by 3.3%, but the factor income - net value added at factor cost - per annual working unit increased by 21%, because of the subsidies on production increased by 3.4% for 2010-2016. In EU-28, the factor income per annual working unit increased, but most of this income was for developing agricultural production technology.

To determine the best phosphorus (P) fertilizer management strategy in chernozem soil in the semi-arid region of Northeast China, a field experiment under film mulched drip irrigation was conducted for two consecutive years. Five P application methods were tested, including no P fertilizer applied (P0); P fertilizer one-time basal application (P1); one fertigation one time (P2); fertigation twice (P3) and fertigation four times (P4). The shoot dry matter weight, phosphorus accumulation, yield and POlsen in soil were measured regularly during the maize growing season. The results demonstrated that P fertilizer application methods imposed significant effects on dry shoot matter, the apparent P loss, P fertilizer use efficiency and yield of maize (P < 0.05). The yield, P agrinomic efficiency and P recovery efficiency of P4 treatment were significantly higher than P1 treatment by 4.2, 39.7, and 66.4% across two year. In addition, P4 treatment significantly enhanced the shoot dry matter weight after V12 stage, post-silking P uptake and reduced the apparent P loss. In conclusion, P fertigation and a rational frequency (e.g., fertigation four times) enable to keep the maximum grain yield, the shoot dry matter, and recovery efficiency of P fertilizer (66.4%) though changing agronomic methods for efficient acquisition of P toward a sustainable and productive agricultural fertigation system.

We investigated the changes in omega 3 enriched carp fillets caused by pan frying. The investigated characteristics were fat uptake, fatty acid (FA) composition, and oxidation. Four different fats were used and fillets were fried plain or battered. The fillet fat content increased during frying and FA composition in the fillets reflected the composition of the frying fat. Frying with sunflower oil negatively influenced the nutritional value by decreasing the n-3/n-6 ratio in the fillets. Frying with rapeseed oil preserved the favourable n-3/n-6 ratio without increasing the saturated fatty acids (SFA). Frying with lard and butter preserved the n-3/n-6 ratio but increased the SFA content. No increased oxidation occurred with the use of rapeseed oil. We concluded that using rapeseed oil for fish seemed to preserve the nutritionally valuable composition best.

Turnip mosaic virus (TuMV) is the most important virus of brassica crops. In our study, we compare the genetic structure of two Czech TuMV populations sampled in the country's 25-year interval of virus presence. The 21 isolates, mainly infecting rutabaga and horseradish, were collected from four farms under organic production, and nearly complete genome sequences, 9 596–9 787 nt in length, were obtained using Sanger sequencing for all of them. The analysis of variability and polymorphism showed differences in genetic structure but the relative stability of both populations and moderate negative selection as a factor affecting the current TuMV population. The newly collected isolates are characterised by a relatively high frequency of intralineage recombinants; interlineage recombinants were not detected compared to the 25-year-old population. The phylogenetic analysis allowed the classification of all Czech isolates into world-B phylogroup, with the prevalence of isolates of subgroup B2. The spread of isolates belonging to the other phylogenetic groups posing higher phytopathological risk, which were present in the old population and some surrounding countries, was not found.

One of the important issues both in the political discussion about climate change and in forest ecosystem research is carbon sequestration. In this paper, we estimated soil carbon sequestration (SCS) in two Persian oak forest stands of different origin (seed and coppice). Soil samples were taken at two soil depths (0-15 and 15-30 cm) and locations (under the tree crown and open area) in each oak stand. Results showed that surface layers (0-15 cm) had the highest soil carbon sequestration ranging from 41.2 t.ha-1 to 47.9 t.ha-1 for both oak forests. The total SCS was higher (between 79.5 and 89.07 t.ha-1) in open areas of the two forest stands than under the crowns of oak trees. Finally, the amount of total SCS in seed originated forest (SOF) (86.52 t.ha-1) was significantly greater (P < 0.05) than in coppice forest (CF) (77.70 t.ha-1). The results indicate that a relatively large proportion of C loss in CF is due to overgrazing, forest degradation and conversion to coppice forests in the study area.

To analyse the genetic diversity of the Cucumber green mottle mosaic virus (CGMMV) population in Poland and to establish the phylogenetic relationships between the Polish and other isolates described to date, 91 isolates were collected from cucumber plants. The analysis, based on coat protein (CP) gene, revealed the presence of two phylogenetic groups: one consisting of the Polish isolates collected in 2017 and those originated mainly from Asia region and the second including the Polish isolates collected in 2016 and the others from European countries. The sensitive, specific, and rapid one-step loop-mediated isothermal amplification assay was developed for the early detection of genetically diverse CGMMV isolates in seeds and plant material.

The associative effects (AE) between concentrate (C), peanut shell (P) and alfalfa (A) were investigated by means of an automated gas production (GP) system. The C, P and A were incubated alone or as 40 : 60 : 0, 40 : 45 : 15, 40 : 30 : 30, 40 : 15 : 45, 40 : 0 : 60 and 30 : 70 : 0, 30 : 55 : 15, 30 : 40 : 30, 30 : 25 : 45, 30 : 10 : 60, 30 : 0 : 70 mixtures where the C : roughage (R) ratios were 40 : 60 and 30 : 70. Samples (0.2000 ± 0.0010 g) of single feeds or mixtures were incubated for 96 h in individual bottles (100 ml) with 30 ml of buffered rumen fluid. GP parameters were analysed using a single exponential equation. After incubation, the residues were used to determine pH, dry matter digestibility (DMD), organic matter digestibility (OMD), volatile fatty acids (VFA) and ammonia nitrogen (NH₃-N) of the incubation fluid, and their single factor AE indices (SFAEI) and multiple-factors AE indices (MFAEI) were determined. The results showed that group of 30 peanut shell had higher SFAEI of GP_{48 h}, DMD, OMD and total volatile fatty acids (p < 0.05) and MFAEI (p < 0.05) than groups 60, 45 and 0 when C : R was 40 : 60. The group of 10 peanut shell showed higher SFAEI of GP_{48 h}, DMD and OMD (p < 0.05) than groups 70, 55 and 40 and MFAEI (p < 0.01) when C : R was 30 : 70. It is concluded that optimal SFAEI and MFAEI were obtained when the C : P : A ratios were 40 : 30 : 30 and 30 : 10 : 60.

A survey of the pond system in Veľké Blahovo, Dunajská Streda district, Slovak Republic, confirmed the occurrence of tapeworms in carp with a prevalence of 63.5% and mean intensity of 1.7 ± 2.72. The species Khawia sinensis and Atractolytocestus huronensis were identified. We attempted to reduce the occurrence of tapeworms using a medicated feed mixture (granules) with 1% fenbendazole as active substance under controlled conditions. Faeces collected from carp (K2) were examined and the presence of tapeworm eggs was confirmed by the flotation method. Positive carp were divided into three groups; each group, labelled F1 to F3, included 15 individuals. The carp were placed in concrete pools (storage ponds) nourished by fish pond water at the peak of the vegetation period from August 7-17, 2015. The medicated feed mixture was administered to the first two groups (F1 and F2), while the third group was fed with a non-medicated feed mixture and served as the control (F3). A single dose was administered to the F1 group; tapeworm prevalence 24 hours after administration in the F1 group was 26.66% with a mean intensity of 1.3 ± 0.57. The F2 group was fed three times in total with 24-hour intervals between feeding. Tapeworm prevalence 24 hours after the last administration in the F2 group was 13.33% with a mean intensity of 2.0 ± 0.77. The prevalence in the control group was 66.66% with a mean intensity of 2.2 ± 1.62. Khawia sinensis tapeworms responded well to fenbendazole, while A. huronensis tapeworms responded poorly and, despite repeated administration of the active substance, this species remained attached to the intestinal mucosa. When compared with the control group, the reduction in the number of tapeworms was statistically significant (P ≤ 0.05) in the F2 group; however, it was not statistically significant in the F1 group.

Research in southern Italy assessed the effects of biodegradable mulch on fruit yield and quality of two greenhouse tomato cultivars, 'Coronel F₁' and 'Kero F₁'. Three mulching types (two MaterBi biodegradable black films, MB N2/12 amnd MB N8; black polyethylene film, low-density polyethylene (LDPE)) and not mulched control were compared. 'Coronel F₁' showed higher values of fruit yield, total crop biomass and leaf area index (LAI). MB N8 and LDPE films led to the highest fruit yield and growth indexes, whereas not mulched control to the lowest. Fruit dry residue and soluble solids were highest under MB N2/12 and MB N8, titratable acidity was highest under MB N8. Fruits grown under MB N8 and LDPE mulches attained the highest levels of colour components "L" and "b" respectively, and MB N8 the highest fruit firmness. MB N2/12 and MB N8 showed the highest levels of antioxidants and antioxidant activity. Biodegradable polymers improved root growth conditions and fruit quality, showing suitable features for sustainable vegetable production.

Nowadays, quadrupedal locomotion information is well established for dogs, horses, and pigs, and kinetic systems have been widely used for sensitive and reliable gait analysis. However, few studies have investigated cat locomotion and the data available are confusing and restricted, especially in relation to jumping. In addition, it has been suggested that several factors, including the influence of the body weight (BW), may be associated with the collection of the kinetic data measurements in dogs. This raises the question of whether the BW would influence the kinetic data measurements in cats. Therefore, this study was aimed at comparing the kinetic parameters of the landing during the jumping in cats with different BWs and to determine the associations between the BW and the kinetic parameters. Twelve client-owned cats were sub-divided into two groups based on the BW and were categorised as a thin group and a heavy group. Each cat was encouraged to jump from a table (1.0 m) onto a force plate several times. The trials were considered to be valid if the cat jumped normally onto the plate and then continued to walk forward. The kinetic parameters including the peak vertical force (PVF) and the vertical impulse (VI) were obtained for each limb. In addition, correlations between the PVF, VI, and BW and the symmetry index (SI) of the forelimbs and hindlimbs were also determined. Most of the kinetic parameters of the thin cats were significantly smaller than the heavy cats during the landing and these values increased as the BW increased, while the normalised PVF and VI of the forelimbs were significantly smaller in the heavy cats than in the thin cats. In addition, for both groups, the non-normalised or normalised PVF and VI were significantly larger in the forelimbs than the hindlimbs, and the SI of the PVF was significantly smaller at the forelimbs than in the hindlimbs. In conclusion, the results of this study showed substantial similarities and differences during the landing between thin and heavy cats. These findings should provide more reference data for the biomechanical motion analysis related to jumping in clinically intact cats.

The draft Strategic plan of the Common Agricultural Policy (CAP) for the Czech Republic has provoked the displeasure of many entrepreneurs in agriculture due to the redistributive payments, to which 23% of the total volume of direct payments will be directed instead of the originally planned 10%. According to the creators of the Strategic plan, this should support the fairer distribution of payments, respecting the benefits arising from the scale of production of large companies. Critics of the plan claim that operating subsidies are already degressive and fear an adverse impact on medium-sized companies, which, given the structure of the companies in the Czech Republic, will affect a large part of agriculture. This article aims to identify the farm size for which direct payments will be reduced and the likely degree of the impact on the farm economy. Our results suggest that the new payment system will lead to a reduction in direct payments for farms larger than 313 ha and, from a farm size of 873 ha, direct payments will fall below 85% of the average, which may cover around 50% of the agricultural land in the Czech Republic.

In recent years, progress has been made towards incorporating the sociocultural perspective in the assessment of ecosystem services (ES) to identify the relevant services according to the different needs and perceptions of the populations consulted. New sociocultural assessment methods are being explored to respond to this emerging line of research, without a generally accepted alternative being found thus far. Aiming to contribute to this line of research, this article proposes a new methodological approach for the sociocultural assessment of ES. The new methodological approach is based on combining two different measurement procedures: a traditional Likert scale and the Kano model. The case of ES provided by forests will be considered for its development and application. With the application of the proposed methodology, the sensitivity and consistency of sociocultural assessments of ES would be improved, and the development of longitudinal analysis would be facilitated.

Political and security stability have the potential to support developing countries‘ efforts to achieve Sustainable Development Goals, as this region is home to the majority of the global population. This study examines the impact of political and security stability and other factors on food security [as measured by the prevalence of undernourishment (PUN)] in developing countries. We analysed 2002–2020 data from 83 developing countries across Africa, Asia, and Latin America and the Caribbean, using the system General Method of Moment (sys-GMM). Our study indicates that political and security stability and the use of the internet can increase food security or reduce PUN in developing countries. Other factors contributed to an increase in PUN: the previous year’s PUN, the cereal import dependency ratio and the unemployment to total population ratio. Meanwhile, other factors have varying effects on the PUN in developing countries.

The need to reduce the content of questionable health preservatives leads to the search for new methods to extend the shelf-life of meat products. The spectrum of possible approaches includes physical methods and the use of additives from natural sources. In this study, we examined the influence of the combination of high-pressure processing (HPP) and the addition of natural antimicrobials on the shelf-life of cooked ham. The samples of cooked ham were produced in a professional meat processing plant. One half of the samples were produced according to a traditional recipe, and the other was enriched with potassium lactate in the form of a commercial product PURASAL^® Hirer P Plus. This product is produced via sugar fermentation and contains high levels of potassium lactate, a compound with high antimicrobial activity. Cooked hams were inoculated by bacteria Serratia liquefaction, vacuum packaged and treated by HPP. Packaged ham samples were stored at 3°C for 40 days and the total microbial count was examined during this storage period in defined intervals. The combination of HPP and potassium lactate from natural sources significantly reduced the total microbial counts in cooked hams and, thus, could be a suitable solution for the meat industry.

Eight less-known juices are characterised and contents are monitored of selected substances and nutritional parameters. 100% juices were produced of Aloe Vera, Aronia, Blackcurrant, Black elder, Cranberry, Malpighia, Pomegranate and Seaberry by pressing, including the flesh. Total phenolic compounds (TPC) and ascorbic acid (AA) content were analysed as other parameters. The juices were measured on total antioxidant capacity (TAC) as the content of trolox, where the highest levels were found for Malpighia 63.2 mM, when the value was significantly different (P < 0.05) compared with all the other juices, while Aloe Vera had lowest levels with 0.4 mM. The Aronia juice possessed the highest level of TPC (8297 mg of GAE/l). TAC levels very well corresponded with TPC levels while AA content actually did not correlate with TAC values. In addition to the objective analysis, the juices were subjected to a sensory evaluation. Blackcurrant presents an attractive product from the sensorial aspect as well as in terms of the nutritional value.

The host resistance level of pure line materials was assessed in the genepool for the purpose of breeding. The highest resistance to the pathogen was observed in bell-type pepper. Moreover, genetic diversity of pure lines was investigated using selected inter-simple sequence repeat (ISSR) primers. Generally, genetic markers showed genetic diversity, so that long-type pure lines were separated from the other accessions. This is the first report on host reactions of Turkish pure lines as breeding material. These results provide significant information for future pepper breeding programs.

The experimental design included seven cover crop species and six kinds of soil tillage in the field cultivation of carrot. The use of cover crops had a positive impact on the yield of marketable roots of carrot in comparison with the cultivation without the cover crops. A significant increase of marketable yield was noted after phacelia, buckwheat, mustard and sunflower. The flat ploughless tillage significantly reduced the marketable yield of roots in comparison with traditional ploughing. The largest marketable yield of roots was obtained from cultivation on ridges after mixing the biomass of buckwheat or phacelia or mustard with the soil, and the smallest, after reduced spring tillage using aggregate without cover crops. The largest marketable yield in flat ploughless tillage was obtained when using grubber before winter, and the biomass of phacelia was mixed with soil. Growing carrot on the ridges had a positive influence on increasing the share of the marketable yield of roots in comparison with other variants of cultivation including the conventional tillage. The all cover crops with the exception of spring vetch significantly increased the share of marketable roots in the yield compared with cultivation without cover crops. The largest percentage of the marketable yield was noted after use of phacelia.

Early identification of mastitis is a serious challenge for dairy farmers and veterinarians in ensuring the health of an animal and the hygienic quality of the produced milk. The purpose of this study was to detect the occurrence and aetiology of mastitis in a dairy herd of 153 milked cows localised in a farm in west Slovakia. During the complex investigation, 606 quarter milk samples were examined (6 quarters were discarded) and classified based on the clinical status, the presence of abnormal udder secretions, the result of the California mastitis test (CMT), the somatic cell count (SCC) and the bacteriological identification of the pathogens causing the intramammary infection (IMI). The study was augmented by the detection of malondialdehyde (MDA) in the milk and the measurements of the blood enzymatic activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) as potential biomarkers for the udder health screening. A positive CMT score was recorded in 19.5% (118) of the examined quarters and 12.5% (76) of the quarters were infected with bacterial pathogens causing latent mastitis (LM; 1.3%), subclinical mastitis (SM; 8.3%), and clinical mastitis (CM; 2.9%). The most commonly isolated bacteria from the infected quarters were coagulase-negative staphylococci (55.2%), Staphylococcus aureus (11.8%) and streptococci (10.5%). The concentration of MDA and SCC were significantly higher from both the SM and CM cases than in the milk samples from the healthy cows, while the blood activities of SOD and GPx were lower in the cows with CM compared to the healthy cows. The higher MDA concentrations in the SM and CM milk observed in this study showed the presence of an oxidative stress in the infected milk, accompanied by a decrease in the antioxidative enzymatic activity in the blood of the cows. Therefore, the measurement of the milk MDA concentration and the activity of the blood SOD and GPx may prove insightful for the better screening of the udder health in the early diagnosis of mastitis.

A number of variables influences potatoes growing, including natural conditions, used growing technologies and market conditions. The most important parameters for the production of potatoes crops are yield, farmer's price, subsidies and costs. All these parameters can change over time. This means that managers of farms must constantly assess the key parameters affecting the economic outturn and analyse the degree of risk of their achievement. This article analyses the economic risks of potatoes cultivation based on statistical data obtained over the last 10 years. The Monte Carlo stochastic simulation method was used to analyse the risk of gross profits. The results of the calculations confirmed the considerable variability and risk of growing potatoes in the climate conditions of the Czech Republic in general, and especially regarding the first early potatoes and potatoes for starch production.

In this study, a global agricultural trade network was constructed, and its topological characteristics from 1991 to 2021 were analysed. The influences of nine variables were empirically tested, including factor endowments and economic, geographical, and institutional factors. The research results show that the scale of trade networks is constantly expanding, and trade relations are becoming increasingly concentrated. Further, global agricultural trade patterns are gradually being reshaped. However, European economies and the United States still dominate the power of network control, with a clear ‘core-edge’ hierarchy. Among the factors influencing the global agricultural trade network, differences in arable land areas, agricultural product prices, geographical distances, and financial institutions have proven important. However, their influence varies. Compared to the differences in the endowments of other factors, the comparative advantage of agricultural trade in various nodes worldwide comes more from the arable land areas. The greater the difference in agricultural prices, the closer are the trade ties between nodes. Differences in geographical distance have proven conducive to establishing agricultural trade relations. Finally, the greater the difference in financial systems, the greater the likelihood that agricultural trade links will occur.

The nutritional status of winter oilseed rape (WOSR) during its vegetative period is crucial for plant growth and can be used for the seed yield prediction. This hypothesis was verified based on the data from long-term field experiments. The experiment consisted of four potassium (K) treatments based on the progressive K supply potential to plants from soil and fertilizer and two magnesium treatments (-Mg, +Mg) conducted in 2013-2015. The content of nutrients (N_tot, P, K, Mg, Ca, Fe, Mn, Zn, Cu) was determined at the rosette stage (BBCH 30) for leaves and separately for leaves and stems in the late stage of inflorescence growth (BBCH 57-59). The low K content appeared as the key limiting nutrient in WOSR plants in the rosette stage due to the insufficient soil fertility level, depended even more on weather conditions. This negative K nutritional trait persisted through the whole vegetative WOSR growth. Its detection was possible, because stems were included in the diagnostic procedure. The most reliable prognosis of WOSR yield was conducted based on the nutritional status of stems in the late stage of the inflorescence development.