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Different solvents and extraction solutions (distilled water, NaCl, Tween 80, citrate buffer, and acetate buffer) were evaluated for enzymes recovery. The independent variables evaluated were agitation (12-348 rpm), time (4.8-55.2 min), and temperature (13.2-46.8°C) and 10 extraction cycles, using an experimental design (Central composite rotatable design 2³). Pectin methyl esterase maximum recovery by solid state culture of Aspergillus niger was 11 U/g_wm (31 U/g_dm) using NaCl (0.1 mol/l) as solvent at a 5 : 1 (v/w) ratio, 30°C for 55.2 min, at 180 rpm, with one extraction cycle.

The efficacy of Bt maize MON 810-YieldGard^® and of Trichogramma wasp against European corn borer (ECB) (Ostrinia nubilalis Hübner) was evaluated in the period of 2002-2008 in field trials on three localities in the Czech Republic. The efficacy of Bt maize on the reduction of the number of tunnels caused by ECB per 100 maize plants before harvest was always 100% and that in Trichogramma treatment was on average 50%. The mean increase of the yield of 15% and 10% was obtained in Bt maize and Trichogramma treatments, respectively in comparison with the untreated control. The damage curve and economic injury level by ECB on maize was developed for the evaluation of the yield losses and management of the pest control. The higher economic efficacy of growing Bt maize as compared to other control measures is documented.

Temporal changes in the concentrations of plant-available phosphorus (P) in soil (Olsen-P), total soil-P and P activation coefficient (the ratio of Olsen-P to residual-P (i.e. an approximation to total-P)) were measured in plots that received consistent inorganic nitrogen, phosphorus and potassium plus organic fertilizers annually. Maize and winter wheat crops were grown in rotation for 24 years. Olsen-P and P activation coefficient declined significantly in the earlier years (< 12 years) for treatments that did not include any P fertilizer, and increased over the same period for the P-fertilized treatments. The rates of change in the Olsen-P and P activation coefficient values were positively related to P balance. In the later years, the Olsen-P and P activation coefficient plateau values were positively related to the P balance.

The objectives of this study were to estimate linkage disequilibrium (LD), describe and scan a haplotype block for the presence of genes that may affect milk production traits in Portuguese Holstein cattle. Totally 526 animals were genotyped using the Illumina BovineSNP50 BeadChip, which contained a total of 52 890 single nucleotide polymorphisms (SNPs). The final set of markers remaining after considering quality control standards consisted of 37 031 SNPs located on 29 autosomes. The LD parameters historical recombinations through allelic association (D') and squared correlation coefficient between locus alleles frequencies ( r²) were estimated and haplotype block analyses were performed using the Haploview software. The averages of D' and r² values were 0.628 and 0.122, respectively. The LD value decreased with increasing physical distance. The D' and r² values decreased respectively from 0.815 and 0.283 at the distance of 0-30 kb to 0.578 and 0.090 at the distance of 401-500 kb. The identified total number of blocks was 969 and consisted of 4259 SNPs that covered 159.06 Mb (6.24% of the total genome) on 29 autosomes. Several genes inside the haplotype blocks were detected; CSN1S2 gene in haplotype block 51 on BTA 6, IL6 and B4GALT1 genes in haplotype blocks 6 and 33 on BTA 8, IL1B and ID2 genes in haplotype blocks 19 and 29 on BTA 11, and DGAT1 gene in haplotype block 1 on BTA 14. The extension of LD using BovineSNP50 BeadChip did not exceed 500 kb and its parameters r² and D' were less than 0.2 and 0.70, respectively, after 70-100 kb. Consequently, the 50K BeadChip would have a poor power in genome wide association studies at distances between adjacent markers lower than 70 kb.

The development of Podzols is conditioned by many factors. One of them is vegetation cover. The aim of this study was to examine in detail special chemical properties, micromorphological features and water retention ability of Podzols under two different vegetation covers (spruce forest and grass). The study was performed in the Jizerské hory Mts., which were strongly influenced by atmospheric acidificant depositions in the past. The study was focused on the assessment of a 30-year grass amelioration impact on soils on the former forest land. It was shown that larger differences in the studied chemical properties (pH_KCl, pH_H2O, eCEC, content of Ca, Mg, Al_KCl, Al_H2O, Al(X)¹⁺, Al(Y)²⁺ and Al³⁺ species) were in the surface organic horizons and decreased with depth. Podzolization intensity was higher under the spruce forest than under the grass cover. Higher amounts of potentially dangerous Al forms were detected in the soils under the spruce forest than under the grass. Grass expansion on clear-cut areas (former forest) as a natural amelioration step results in the particular restoration of soil conditions. The micromorphological features studied on the soil thin sections using the optical microscope and soil water retention curves measured on the undisturbed 100 cm³ soil samples showed a significant influence of the organic matter presence on the soil structure and retention ability of H and Bhs horizons. Soil under the grass cover had denser structure (e.g. greater fraction of small capillary pores) and higher retention ability than soil under the spruce forest. Very similar retention curves were measured in the Ep and Bs horizons under both vegetation covers. Micromorphological features studied on the thin soil sections clearly documented a podzolization mechanism (e.g. organic material transport and its accumulation, weathering process and Fe oxidation and mobilization).

The paper is devoted to an analysis of the development of agrarian farms in Visegrad Group (V4) countries, primarily in terms of results and the most important production inputs of production factors and their efficiency in the period from 2004 to 2013 based on the EU Farm Accountancy Data Network (FADN). The results of the analysis show that if farms in the V4 countries want to achieve the same performance as developed countries, they must invest more in purchasing intensification factors and adjust the structure of assets production specification. It will be necessary to stop the reduction in the numbers of livestock and to strive for growth in gross farm income, mainly through the processing of agricultural raw materials.

Off-flavour in soft drinks is one of the main threats to manufacturers, which can result in expensive recalls, and discredit the brand. Off-flavours can occur for a variety of reasons (e.g . chemical contamination of raw material). The presented case study has proved there is a relationship between the identification of biphenyl and its derivatives in the used preser vative (benzoate) and off-flavours in the drink . The project consisted of three phases: (1) the assessment of the probable cause of off-f lavours based on sensor y evaluation and GC-MS -Olfactometr y profiling of volatiles; (2) the quantification of biphenyls and the characterisation of their sensor y properties; (3) the screening of commercially available benzoates for the presence of biphenyls. Based on the odour threshold obtained by GC-O (0.03 mg/l for 4-methyl-1,1'-biphenyl and 0.02 mg/l for biphenyl in an aqueous solution) and the common benzoate content in soft drinks (0.14 g/l), the 'non-obser vable sensory' levels of contamination were determined to be maximally 0.143 mg/g of benzoate for biphenyl and 0.214 mg/g of benzoate for 4-methyl-1,1 '-biphenyl .

The aim of this study was to determine the effect of yeast harvest timing on the process performance, total yeast count and the content of volatile components in beer. The experiments were performed on an industrial scale with fermentation and maturation conducted in three fermentation tanks with a capacity of 3800 hl (cylindro-conical tanks - CCT). All processes were carried out using the same technological conditions. The worts were aerated with sterile air and yeast after the second fermentation (third generation) was added. The duration of the maturation phase and the processes of the yeast harvest were conducted at different times (1^st, 4^th and 6^th day) after finishing the primary fermentation process. During fermentation and maturation, changes in the contents of the extract, yeast, and volatile components were investigated. These experiments showed that the use of different times during yeast harvest had a significant impact on the course of fermentation and maturation and impact on the total yeast count during the maturation process and on the amount of volatile components in beer. With a delay in the start of yeast cropping, the content of acetaldehyde and vicinal diketones decreased and the content of esters increased. The timing of the yeast crop significantly influenced the final beer quality.

Dimethyl sulphoxide has a variety of biological effects that have made it the subject of numerous pharmacological studies. The first dimethyl sulphoxide therapeutic indication approved by the United States Food and Drug Administration (FDA) in 1978 was for intravesical instillation in cases of interstitial cystitis. Since then, due to its distinctive properties, it has been the subject of studies in several areas. This review describes indications, adverse effects and contraindications, as takes a critical approach to the main articles addressing the clinical use of dimethyl sulphoxide.

The present paper summarized some knowledge of modern technologies, applied in apple (Malus domestica) genome studies. New generation sequencing allowed single nucleotide polymorphism (SNP) chip technologies for genotyping, description of functional apple genes, characterization of the evolutionary results in apple genome fragment transition, as well as phylogenetic reconstruction of the genus Malus, being the confirmed progeny of M. sieviersii. Based on these technologies, newly developed putative markers may give the most important biological data such as age, geographical origins, tissue type determination, and external visible characters. The new generation genotyping platforms, representing very high efficiency, are now successfully applied for random apple genome-wide association (GWA) studies as well pedigree-based analysis and marker-assisted selection (MAS).

Downy mildew of sunflower is a recurrent threat for crop production and a permanent challenge for resistance breeding. Since 2016, a new pathotype of the oomycete Plasmopara halstedii has been found in fields of cutting sunflower in Southern Germany. Infection assays based on sunflower differential lines identified the pathogen as pathotype 354 which has not yet been described anywhere else. The new pathotype readily infected the host lines Ha-304, RHA-265, PM13, 803-1, and Ha-335, whereas RHA-274, PM17, HAR-4, and QHP1 remained uninfected. It is the first isolate of Plasmopara halstedii to overcome the Pl-6 resistance of sunflower in Germany.

Excessive nitrogen (N) and water input, which are threatening the sustainability of conventional agriculture in the North China Plain (NCP), can lead to serious leaching of nitrate-N (NO₃^--N). This study evaluates grain yield, N and water consumption, NO₃^--N accumulation and leaching in conventional and two optimized winter wheat-summer maize double-cropping systems and an organic alfalfa-winter wheat cropping system. The results showed that compared to the conventional cropping system, the optimized systems could reduce N, water consumption and NO₃^--N leaching by 33, 35 and 67-74%, respectively, while producing nearly identical grain yields. In optimized systems, soil NO₃^--N accumulation within the root zone was about 80 kg N/ha most of the time. In the organic system, N input, water consumption and NO₃^--N leaching was reduced even more (by 71, 43 and 92%, respectively, compared to the conventional system). However, grain yield also declined by 46%. In the organic system, NO₃^--N accumulation within the root zone was generally less than 30 kg N/ha. The optimized systems showed a considerable potential to reduce N and water consumption and NO₃^--N leaching while maintaining high grain yields, and thus should be considered for sustainable agricultural development in the NCP.

Soil erosion by water has become an issue in the cultivation of maize (Zea mays L.) on sloping lands in recent years. The following three technologies of tillage have been assessed: disc cultivator, strip-till and no-till (raw land). Seeding machine Kinze 3500 was used for sowing maize cultivar Silvinio FAO 210. The experiments were conducted within the years 2013, 2014 and 2016. Erosion was evaluated under simulated rain in three stages of maize growth. The following parameters of each tested tillage treatment were measured: water infiltration (mm), soil loss (converted to t/ha), dry matter yield of the aboveground biomass and grain yield. The results confirmed that no-till technology reduced water erosion to the value of 0.40 t/ha. This technology along with the row spacing of 0.75 m tended to bring higher yields of aboveground biomass (13.40 t/ha). Tillage and phacelia as a catch crop increased water infiltration in the soil.

The ribosomal protein SA (RPSA), also known as 37-kDa laminin receptor precursor/67-kDa laminin receptor (LRP/LR), has been identified as a multifunctional protein, playing an important role in multiple pathologies like cancer and prion diseases. Since RPSA is involved in the binding and internalization of the prion protein, mutations in the ovine RPSA gene, influencing the RPSA-PrP^C/PrP^Sc binding, can potentially play a part in the resistance to prion diseases. Our goal was to further characterize the complex RPSA gene family and to detect structural mutations which can play a role in this disease. In a prior study, 11 ovine pseudogenes were detected experimentally. As the whole genome shotgun ovine genome became accessible, an in silico genome-wide screening was performed and 37 new pseudogenes (36 processed and one semi-processed pseudogene) were detected, bringing the total to 48 ovine RPSA pseudogenes. Additionally, the complete bovine genome was screened in silico and 56 pseudogenes were identified. Once these sequences were known, it was possible to analyze the presence of mutations in the coding sequence and exon-flanking regions of the ovine functional full-length RPSA gene without the interference of pseudogenic sequences. Nineteen mutations were found: one in the 5' UTR, a silent one in the coding region, and seventeen in the exon-flanking regions, including an interesting mutation in the SNORA62 gene, localized in intron 4 of RPSA, leading to potential ribosomal defects. Structural mutations of the RPSA gene can be ruled out to play a role in transmissible spongiform encephalopathies but regulatory mutations still can have an effect on these diseases.

The paper presents selected results of the sociological research focused on social inclusive activities that use and are located in the natural environment of rural areas. The paper is based on the contemporary form of the concept of a fully integrated modern society, and deals with the implementation of this concept within the strategy of social inclusion in both the European and Czech context. The authors have created, based on secondary research, a typology of social inclusive activities within Czech rural areas. Primary research, consisting of a questionnaire survey, was used to study the awareness of these issues among the rural population. Based on the selected results of both the primary and secondary research, the authors conclude that a possible path to social inclusion, is, alongside specific social services, social farming, which harmonizes the potential of the rural natural environment with the attitudes of the rural population (the actors and observers of social inclusive activities within their locality).The ongoing research is focused on monitoring barriers to the development of this business sector, which are crucial for the rural actors.

Hepatitis A and E viruses (HAV and HEV) are recognised as food- and water-borne infections and shellfish are a well-documented source of these viruses. The presence of HAV and HEV in mussels has not previously been investigated in Bosphorus, Istanbul, Turkey, and therefore the aim of this study was to investigate the frequency of HAV and HEV in mussels collected from this location. A total of 92 pooled samples representing 736 mussels originating along the Bosphorus coast were collected from fish distributors. RNA was extracted using the RNeasy Plant Mini Kit and a TaqMan real-time RT-PCR was performed using primers specific to HAV and HEV. Amongst the 92 pooled samples tested, 3 (3.3%) were found to be positive for HAV by the TaqMan real-time RT-PCR. No HEV was detected in any of the mussels tested. A 174 bp product was observed on gel electrophoresis with HAV positive samples detected by SYBR Green real-time PCR after reverse transcription. This product was sequenced and confirmed to contain HAV sequences by alignment using the data in GenBank. This is the first report describing the presence of HAV in mussels harvested in the Bosphorus, Istanbul, Turkey, indicating the potential contamination of aquatic environment and risk to public health.

Foreign bodies that affect airways are a well-recognised problem in small-animal practice. Cats in par­ticular, may be especially at risk of aspiration due to their predatory behaviour. Here, we report a case of an insect as a foreign body, namely a beetle in the tracheal bifurcation of a cat. Radiographs suggested a wide range of possibilities for differential diagnosis, but the presence of a foreign body was initially suspected. A final diagnosis was not pursued by the owner and the cat was euthanised. The presence of a summer chafer was then confirmed. To the authors' knowledge, this is the first time such a foreign body and radiographic findings are reported in the literature.

We here report the diagnosis and surgical outcomes of a cat and three dogs with persistent right aortic arch causing oesophageal enlargement. The chief complaint in all cases was regurgitation just after solid food intake. Barium oesophagographic examinations revealed enlarged oesophaguses cranial to the heart and strictures due to arteriosus ligament constriction of the oesophagus. Surgical correction was carried out using left fourth intercostal thoracotomy and severance of the ligamentum arteriosum. The cases frequently displayed residual clinical signs and still required modified diets after a mean follow-up period of 8.6 months, but owner satisfaction was acceptable.

The objective of the present work was to determine changes in total sugars, reducing sugars and sucrose content in potato tubers under conditions involving an application of biostimulants and herbicides. Research material included table potato tubers obtained in a three-year field experiment. The experiment was a split-plot design with three replicates. The factors were as follows: factor I - three table potato cultivars (Bartek, Gawin and Honorata), factor II - five methods of biostimulant and herbicide application (1. control - without biostimulants or herbicides; 2. Harrier 295 ZC (linuron + chlomazon); 3. Harrier 295 ZC (linuron + chlomazon) and growth regulator Kelpak^®SL; 4. Sencor 70 WG (metribuzin); 5. Sencor 70 WG (metribuzin) and growth regulator Asahi^®SL. Variance analysis demonstrated that total sugars, reducing sugars and sucrose content were affected by the biostimulants and herbicides applied as well as cultivars and weather conditions. Compared with control, a significant increase in total sugars followed an application of Sencor 70 WG as well as Sencor 70 WG and the growth regulator Asahi^®SL whereas reducing sugars and sucrose content significantly increased after Sencor 70 WG and the growth regulator Asahi^®SL were applied.

Influence of plant growth regulators chlormequat chloride, chlormequat chloride + ethephon, ethephon, and mepiquat chloride + prohexadione-Ca + pyraclostrobin + ammonium sulphate (BAS67800F + BAS00800D) on decreasing sunflower height was evaluated. It was determined that sunflower height can be reduced by as much as 30 cm. In the case of BAS67800F + ammonium sulphate, there was a slight difference between application at BBCH 31-33 and BBCH 50-51, whereas for ethephon better application time was at BBCH 50-51. For chlormequat chloride, application at BBCH 31-33 was better, but height reduction did not endure until harvest. Flower head diameter shortly before harvest was not affected at any tested regulator. Flowering was delayed primarily at applications at BBCH 50-51.

Lamb growth performance traits in relation to parental breeding values (BVs) for these traits were evaluated in a purebred Suffolk sheep population in the Czech Republic. The research lasted over 8 years and included 24 886 lambs. Four relevant parental BVs were observed: BV predicted for lamb live weight direct effect (BVLW-DE), BV predicted for lamb live weight maternal effect (BVLW-ME), BV predicted for lamb musculus longissimus lumborum et thoracis depth (BV-MLLT), and BV predicted for lamb backfat thickness (BV-BT). The lamb live weight (LW; kg), musculus longissimus lumborum et thoracis depth (MLLT; mm), and backfat thickness (BT; mm) were assessed at 100 days of age. A dataset was created using the most current parental BVs for each year (2007-2014) and subsequent growth traits of their lambs in the next season (2008-2015). Linear regressions showed an increased tendency when one point in dam BVs was associated with an increase in lamb LW (0.393 kg; P < 0.01 in BVLW-DE and 0.090 kg; P < 0.05 in BVLW-ME), MLLT (0.340 mm; P < 0.01 in BV-MLLT), or BT (0.243; P < 0.01 mm in BV-BT). Lower (but significant - P < 0.01) values on linear regression were detected for sire BVs, when 0.135 kg of LW, 0.217 mm of MLLT, and 0.214 mm of BT corresponded to 1-point increases of BVLW-DE, BV-MLLT, or BV-BT. This was confirmed by ANOVA evaluation, especially for LW and MLLT traits. Maximal differences (P < 0.05) in lamb LW were 1.84 kg or 0.88 kg regarding to dam or sire BVLW-DE groups. Similarly, the difference (P < 0.05) in lamb MLLT reached 0.82 mm in dam BV-MLLT, while 0.57 mm was detected in sire BV-MLLT groups. These results have practical implications for the objectives of selection schemes used in the Suffolk sheep population in the Czech Republic.

This 12-year study was conducted to evaluate the performance of new plum cultivars from the Czech Republic. A new cultivar, 'Stáňa', was the most productive, followed by 'Kamir' and the standard cultivar 'Stanley'. The new plum cultivar 'Stáňa' also had the highest mean yield per cubic meter of tree canopy. Next in order of production efficiency were 'Stanley', 'Amátka', 'Kamir' and 'Čačanska lepotica'. According to canopy volume, the most vigorous cultivar was 'Kamir' followed by 'Samera' and 'Stáňa'. The least vigorous was 'Dwarf', having a canopy volume distinctly smaller than 'Amátka', which was next in the order. The shape of tree canopies ranged from 'Dwarf', which had the most upright canopy, to 'Simona' with a more distinctly overhanging canopy at the opposite extreme. The highest percentages of fruiting on first and second year wood were observed on trees of 'Simona' and 'Čačanska lepotica'.

Germinated seeds are rich in various nutrients but are vulnerable to fungal contamination which favours micromycete formation on the sprouts. The main aim of this work was an investigation of strategies to reduce the contamination of sprouted seed foods. Over the course of 96 hours of sprouting, seeds of organic spring wheat (Triticum aestivum L.), winter wheat (Triticum aestivum L.), naked oat (Avena nuda L.), triticale (xTriticosecale) and rye (Secale cereale L.) were irrigated in water filtered using the Pazdroid Med-1500 filtration device with and without 4% ethyl alcohol. Germinated seeds were stored at 18°C for one, three and seven days and the levels of Mucor spp., Penicillium spp., Alternaria spp., Aspergillus spp., Fusarium spp. and Bipolaris spp were determined. Micromycete numbers were greater in sprouted winter wheat and rye but were reduced when these were soaked and irrigated with filtered water and filtered water containing 4% ethyl alcohol. Filtered water led to greater reductions in micromycete numbers in sprouted winter and spring wheat than in other seeds.

The colour, chemical composition, texture parameters, hydration properties and fatty acids profile of fresh wild boar meat and meat after 2 months of freezing were compared. The research material was the M. longissimus dorsi muscle cut out of wild boar carcasses (Sus crofa). Analyses of physical and chemical properties of samples taken from 16 boars were performed. Samples were packed and frozen to -18°C and stored under such conditions for a period of two months. Freezing storage significantly increased the elasticity value and the colour of the meat as indicated by the decrease in L* and b* values. In frozen meat there slightly increased thermal drip and forced drip. The changes in the fatty acid profile under the effect of freezing were found more often in fat from muscle M. longissimus dorsi than in back fat of wild boar. In conclusion, it should be noted that freezing storage in a short time does not affect negatively the quality of frozen meat.

Tritrichomonas blagburni (previously called T. foetus) has been implicated as an aetiological agent of long-term large-bowel diarrhoea in cats in many countries worldwide. The aim of this study was to determine the presence of, and risk factors for T. blagburni among a cohort of cats living in different conditions in the Czech Republic. Samples were collected from 170 cats living in different environments. The InPouch™ TF-Feline medium method was used for diagnosis of feline trichomonosis. A single case (0.6%) with motile trichomonads identified as Pentatrichomonas hominis was found in a cat from a multi-cat household. Our study suggests that trichomonads and in particular, T. blagburni, infection may be much less common in the Czech Republic than in neighbouring countries, despite the inclusion of cats that were likely to be from higher-risk groups. A review of studies of the association of trichomonads and feline diarrhoea carried out in different countries revealed variation in the frequency of trichomonads detected. Different combinations of PCR or culture methods for screening or confirmation have been utilised, with or without species differentiation; however, this could not solely account for the variation in the occurrence between countries. From those studies where differentiation was performed, we calculated from the combined studies that T. blagburni occurred in six cats without diarrhoea (1.1%) and 47 cases with diarrhoea (5%). This finding supports an association with diarrhoea as well as the occurrence of asymptomatic cases. We note that in many studies, including our own, the occurrence of T. blagburni may well be underestimated and suggest that future studies use a combination of PCR screening of both faeces and faecal cultures, with differentiation of trichomonad species.

The epidemiological situation regarding the infectious bronchitis virus (IBV) population in Europe as well as the presence of predominant IBV strains is well described. The aim of this epidemiological study was to describe the real field situation in the Czech Republic and Slovakia, as no data are available for the last ten years. The study was also focused on differentiation between field IBV strains and vaccine/vaccine origin IBV strains in different poultry segments including backyard flocks. Between July 2013 and July 2016, cloacal, tracheal and/or visceral swab samples were collected from 145 Czech and Slovak chicken broiler, breeder and layer flocks. The majority of flocks was kept for production purposes, but to enable a more complete picture of the situation in the field backyard flocks with more than 50 birds were also included. As in other cases which were reported worldwide and based on collaboration with x-Ovo laboratories, samples were analysed using the real-time polymerase chain reaction (RT-qPCR) to detect the presence of the RNA of IBV. When positive, approximately 400 base pairs encoding the hypervariable region of the IBV S1 protein were sequenced. Sequencing results, cycle threshold values and vaccination history were used as criteria to try and distinguish vaccine strains from field strains. A significant percentage of all flocks presented clinical signs suggestive of IBV infection. From the total number of samples examined, 16.5% were negative. In 12.4% of the samples that did contain RNA from IBV, the genotype could not be determined. In most cases, this was due to the recovery of RNA quantities below the lower limit of detection of the sequencing PCR. The remaining positive samples predominantly contained RNA from IBV strains that belonged to the 4/91 - 793B - CR88 (44.7%), Massachusetts (30%), D274 - D207 (11.6%) and D388 - QX (8.7%) genotypes. Estimations indicated that approximately 23.9%, 48.4%, 58.3% and 0% of these detections, respectively, were vaccine strains. Infections with types UKR/27/2011, CK/CH/Guandong/Xindadi/0903 and K33/09 were observed sporadically. The results confirm that IBV infections are highly prevalent in Czech and Slovak chickens and that at least seven different IBV types were circulating during the monitored period. This underlines the necessity of providing flocks with a strong and broad protective immunity against IBV.

Relation between wheat (Triticum aestivum) nutritional status determined at the beginning of stem elongation and during anthesis, respectively, and available content of phosphorus (P-M3) and zinc (Zn-M3) determined by the Mehlich 3 extractant was studied. Both one-year pot experiment with spring wheat and two-year on-farm trials with winter wheat were run on various calcareous soils (pH values of 7.18-7.94, median 7.80, P-M3 1-289 ppm, median 54, and Zn-M3 2-14 ppm, median 4), in the Czech Republic (Central Europe). Phosphorus nutrition index (ratio of phosphorus concentration in shoot biomass to critical phosphorus concentration - P_c) was calculated using the Belanger et al.'s model: P_c = -0.677 + 0.221N - 0.00292N(2), where both phosphorus and nitrogen concentrations were expressed in g/kg shoot dry matter. Unlike phosphorus concentration in shoot biomass, phosphorus nutrition index significantly correlated with P-M3 content in soil. Optimal values of the phosphorus nutrition index were recorded if P-M3 was 51-68 ppm. Zinc concentration in shoot biomass more strongly correlated with P:Zn ratio (M3) in soil compared to Zn-M3 content in soil. P:Zn ratio in shoot biomass of 130:1 did not lead to phosphorus deficiency and corresponded to P:Zn (M3) ratio in soil of 9.3:1-14.3:1.

In four-year experiments, hop was treated with 7 biologically active substances in two terms during vegetation: Lignohumate max (a mixture of humic acids and fulvic acids), Lexin (a mixture of humic acids and fulvic acids enriched with auxins), Lexenzym (a mixture of humic acids and fulvic acids enriched with auxins, phytohormones and enzymes precursors), Ascophyllum nodosum seaweed extract, synthetic auxin, humic acids and fulvic acids alone. The chlorophyll content was monitored after the application both in the vine leaves and in the branch leaves. After harvesting of the hops from the individual treatments, the yield of dry hops was determined and the cones were analysed for the content of alpha bitter acids. The results show that the most effective hop treatment was the application of Lexin and Lexenzym. The Lexenzym treatment provided a yield of dry hops of 1.86 t/ha, i.e. 0.47 t/ha higher compared with untreated control. The Lexin treatment provided yield higher by 0.41 t/ha of dry hops compared with the untreated control, while the harvested cones contained the most alpha-bitter acids (4.57%).

While it is known that heat treatment of cereal grains generally improves the nutritional value for ruminants, simultaneous information on rumen degradability and intestinal digestibility of the rumen by-pass is scarce, especially for non-starch constituents. The effect of steam flaking at 90°C for 30 min on protein quality of maize, wheat, and barley was studied. In addition to proximal chemical analyses, protein rumen degradability was determined in vitro and intestinal digestibility of rumen undegraded protein was determined using the mobile bag method. No significant effects of steam flaking on chemical composition of cereal grains (crude protein, acid detergent fibre, neutral detergent fibre, and starch) were observed. The protein fractions that are relevant to rumen degradability were significantly influenced by the steam flaking: the non-protein nitrogen fraction (A) was reduced (P < 0.05) by 43-93% for all three cereal grains, whereas the fraction that represents true soluble protein (B1) was reduced (P < 0.05) for wheat grains (by 77%) and barley (by 93%). Although the difference was not significant, a decrease of 41% for B1 fraction was also observed for maize. On the other hand, steam flaking markedly increased buffer insoluble but neutral detergent soluble protein fraction (B2) by 15-25% for all three cereal grains, whereas effects on B3 fraction were not significant. Steam flaking was also associated with an increase of the rumen undegradable protein fraction (C) in the case of barley, from 6.6 to 11.1% of total N (P < 0.05) and wheat, from 5.2 to 8.4% of the total N (P < 0.05). Overall, the changes of the protein fractions led to a considerable increase of the rumen undegradable protein (RUP) for all steam-flaked cereal grains (P < 0.05). Steam flaking also increased (P < 0.05) intestinal digestibility of RUP of the cereal grains (by 3.6-34.8%), leading to a more efficient protein use in ruminants.

This paper describes the process of digitizing Montenegro's legacy soil data, and an initial attempt to use it for digital soil mapping (DSM) purposes. The handwritten legacy numerical records of physical and chemical properties for more than 10 000 soil profiles and semi-profiles covering whole Montenegro have been digitized, and, out of those, more than 3000 have been georeferenced. Problems and challenges of digitization addressed in the paper are: processing of non-uniform handwritten numerical records, parsing a complex textual representation of those records, georeferencing the records using digitized (scanned) legacy soil maps, creating a single computer database containing all digitized records, transforming, cleaning and validating the data. For an initial assessment of the suitability of these data for mapping purposes, inverse distance weighting (IDW), ordinary kriging (OK), multiple linear regression (LR), and regression-kriging (RK) interpolation models were applied to create thematic maps of soil phosphorus. The area chosen for mapping is a 400 km² area near the city of Cetinje, containing 125 data points. LR and RK models were developed using publicly available digital elevation model (DEM) data and satellite global land survey (GLS) data as predictor variables. The digitized phosphorus quantities were normalized and scaled. The predictor variables were scaled, and principal component analysis was performed. For the best performing RK model an R² value of 0.23 was obtained.