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The present study examined the results of using hyaluronic acid with autogenetic cancellous grafts in the treatment of experimentally induced osteochondral defects in the stifle joints of dogs. In this study, 10 mature dogs of different breeds, weights and of both sexes were used. General anesthesia and usual operation procedures were followed. A 10 mm deep defect was created on the femoral sulcus of the trochlea with a drill tip of 8 mm in diameter. The defects in the right and left legs were filled with autogenic cancellous grafts taken from the metaphysial region of the tibia. The left legs constituted the experimental group while the right legs served as control group. In the experimental group, 2 mg/kg intraarticular hyaluronic acid was twice administered into the stifle joint, i.e., immediately subsequent to the operation and 1 month afterwards. Parenteral antibiotics were prescribed postoperatively for ten days. Five animals were sacrificed at the third and sixth month after surgery. Macroscopic and microscopic findings obtained from each case were evaluated. On macroscopical examination, trochlear defects were determined to be incompletely filled at the third month in both control and experimental groups. On histopathologic examination, a loose fibrovascular formation in the area where the graft was applied was observed to be present in both control and experimental groups. However, in the experimental group this formation was more superficial, ossification activity was greater and trabeculous bone formation had been initiated. Macroscopical examination carried out in the sixth month determined that in the control group the defect surface did not fill up to the trochlear sulcus level. In the histopathologic examination, in control groups it was found that fibrocartilageous structures were developing in the fibrovascular space even though ossification was incomplete. The macroscopic examination showed that in the experimental group, the defect surface reached the trochlear sulcus level of defects in this month. The histopatologic examination revealed that fibrous tissue comprised a thin layer, under which ossification processes were complete and bone trabeculates fully formed. It was concluded that the usage of autogenic cancellous graft along with hyaluronic acid may be useful in the repair of large osteochondral defects.

In the year before the accession to the European Union, the Slovak agricultural sector reported a loss of SKK 2.4 billion and following a profitable year, the earnings were reduced by SKK 2.8 billion. The situation was caused by a number of reasons, namely reduced sales of agricultural products, damage resulting from adverse weather effects (cold weather, hail, drought and swine fever), as well as widening of the price gap compared to the year before (increasing input prices in agriculture and decreasing purchase prices of agricultural products, especially in livestock production). Legal entities and natural persons experienced mixed business success. While 51% of legal entities made profit, the figure rose to 76% in the group of natural persons. Both the agricultural cooperatives and trading companies performed with a loss. The loss per hectare of agricultural land (a.l.) was substantially lower in the case of business companies. Natural persons - private farmers were profitable over the period. The gap between the profitable and loss-making enterprises has widened. Some 60% of profitable enterprises owned by legal entities made only a small profit below SKK 0.5 million. The loss-making performance was typical for more productive areas of Slovakia. This was related to stronger effects of adverse climate in 2003.

This paper evaluates the effect of partial root zone drying (PRD) and deficit irrigation (DI) strategies on yield and water use efficiency of the drip-irrigated corn on clay soils under the Mediterranean climatic conditions in Southern Turkey. Four deficit (PRD-100; PRD-75; PRD-50; and DI-50) and one full irrigation (FI) strategies based on cumulative evaporation (E _pan) from class A pan at 7-day interval were studied. Full (FI) and deficit irrigation (DI-50) treatments received 100 and 50% of E_pan, respectively. PRD-100, PRD-75 and PRD-50 received 100, 75 and 50% E_pan value, respectively. The highest water use was observed in FI as 677 mm, the lowest was found in PRD-50 as 375 mm. PRD-100 and DI-50 resulted in similar water use (438 and 445 mm). The maximum grain yield was obtained from the FI as 10.40 t/ha, while DI-50 and PRD-100 resulted in similar grain yields of 7.72 and 7.74 t/ha, respectively. There was a significant difference among the treatments with respect to grain yields (P < 0.01). The highest water use efficiency (WUE) was found in PRD-100 as 1.77 kg/m³, and the lowest one was found in FI as 1.54 kg/m³.

The impact of blue spruce (Picea pungens) and reed Calamagrostis villosa) cover on quantity and quality of upper soil layers was investigated. The research was conducted in the Jizerské hory Mts., Czech Republic (altitude 880 m, acidic spruce forest site type - 8K). Mean weight of dry matter of holorganic horizons was similar under both variants. Totally, there were accumulated 153 t/ha of dry matter of humus horizons in blue spruce and 174 t/ha in reed. Soil pH (KCl) varied from 3.7 to 3.2 under blue spruce stand and from 3.6 to 3.3 under reed. The differences of concentrations of nutrients (P, K, Ca, Mg) were not found significant either. Only L horizon showed significant differences: there were higher values of cation exchangeable capacity (T) and higher content of exchangeable bases (S) under reed. We found very similar forest-floor humus properties under both species. Therefore we can not state worsening of the soil conditions under blue spruce compared to areas covered with tested forest weed species.

High quality of planting material is an essential prerequisite for successful artificial forest regeneration. We carried out a detailed investigation aimed at differences between plantable bareroot and container plants of Norway spruce (Picea abies [L.] Karst.). Based on the results of this experiment, there exist marked differences in basic morphological traits between bareroot plants and plugs. The largest differences were observed in root collar diameter and root system volume. Differences in physiological quality (nutrient content, function of assimilatory organs) were also great. The results document that container seedlings of Norway spruce produced by intensive technology in controlled conditions of plastic greenhouses have very good predispositions for successful growth in difficult mountain conditions.

In this paper is presented the comparison of the selected hydrometeorological data from two experimental micro-scale catchments Modrava 1 (0.1 km²) and Modrava 2 (0.17 km²) in upper parts of Bohemian Forest. These catchments differ mainly in the vegetation cover - a dead forest with very young trees (Modrava 1) and primary forest clearings with 10 to 15-year old young forest (Modrava 2). For comparison were used the data monitored close to the catchments outlets during the hydrological year 2007. Average hourly rainfall and runoff data were analysed. During the winter season, snow water equivalents were measured and the maximum value was added to the rainfall amount measured during the vegetation season for the estimation of total year precipitation on each catchment. The data of the air temperature and water conductivity measured in hourly time intervals were also compared. For the estimation of differences between the monitored data sets the cumulative values of the characteristics observed during whole year were computed. It follows from the comparison of the time series that the time rainfall distribution was similar during the year on both catchments with a higher total year precipitation and hour intensities on the catchment Modrava 2. The time distribution and total runoff depth were similar on both catchments. On Modrava 1 a faster recession of hydrographs could be seen which might relate to a lower retention capacity. The value of the water conductivity on the catchment Modrava 1 depended more on the changes of the runoff depth. The maximal values occurred during the peak discharges or in time of hydrographs rising. This fact can be the result of a lower stability of the soil profile in the catchment with dead forest cover.

A 3.5-year-old Boxer breed bitch was referred to our clinic with a history of progressive and painless inguinal mass on the left side with no significant general symptoms. Ultrasonographic examination of the inguinal mass and abdomen revealed a hypo echoic area covered with a hyper echoic capsule in inguinal mass and also two 40-day-old live foetuses, respectively. During ventral midline laparotomy, an incarcerated left uterus horn was detected in an inguinal hernia. A successful herniorraphy and ovariohysterectomy was performed in one session. A dead foetus was detected histopathologically in the herniated uterine horn. The main differential in this case was a severe incarceration and foetal death in the herniated uterus horn with no significant clinical and ultrasonographic findings. This condition did not affect either the general health condition of the bitch or the foetuses localized abdominally.

: A glasshouse pot experiment tested the effects of 14 different combinations of substrate type, pH and nutrient treatments on the occurrence and severity of leaf chlorosis in a susceptible variety of petunia. Plants grown at optimal pH level (4.7) in peat substrate with low limestone dose were symptom-free even without added micronutrients. Severe chlorosis occurred in plants grown at high pH in peat substrate with high limestone (pH 6.7) and in peat-bark-compost (pH 6.2); it was associated with decreased Fe and Mn content in leaves. Regular application of nutrient solution with low concentration of Fe, Mn, and other micronutrients as EDTA chelates greatly reduced chlorosis in plants grown in peat-bark substrate and in peat-bark-compost, and it improved Fe uptake. An exception was peat substrate at high pH level and high limestone where chlorosis was only partially reduced by this treatment. Regular application of Fe, Mn, and other micronutrients as sulphates or citrates had no substantial effect; only application of three additional substrate drenches of 30 mg/l Mn from Mn-EDTA and 90 mg/l Fe from either Fe-EDTA or Fe-EDDHA substantially corrected chlorosis and increased foliar Fe and Mn in cases where plants were grown in high limestone peat substrate.

This paper addressed the spatial distribution characteristics of organic matter, total nitrogen, extractable phosphorus and extractable potassium in agricultural soils of Northeast China. The related factors were explored using geostatistics and geographic information systems. The results showed that the log-transformed data of the four soil nutrients followed a normal distribution. Soil extractable phosphorus had a higher coefficient of variation. The experimental variogram of the log-transformed data of soil organic matter, total nitrogen and extractable phosphorus was fitted with an exponential model, while soil extractable potassium was fitted to a spherical model. Soil samples from smaller slope gradients had higher organic matter and total nitrogen. Soil type affected the four soil nutrients significantly. Soil samples from dry farming land had significantly higher total nitrogen and extractable potassium than soil from paddy fields, while the contrary was found for extractable phosphorus. Along the Yinma River, soil samples from the western part have statistically higher values for organic matter, total nitrogen and extractable potassium than those collected from the eastern part.

The changes of vitamin C content in relation to Cd, Pb and Zn accumulation in 6 potatoes varieties: very early (Junior, Impala), early (Livera), middle early (Agria), medium-late (Asterix, Desirée) were surveyed in this work. The soil used in pot trial had pseudototal (in soil extract of aqua regia

Tyramine is the most frequent biogenic amine found in cheese and is also frequently found in other fermented foods and beverages. In total 273 different strains of enterococci from various foodstuffs were studied. A multiplex PCR was designed for the genotypic differentiation of various enterococci strains at species level and for determination of the presence of tyramine producing (tyrdc) gene. E. faecalis and E. faecium were found to be prominent strains in dairy and meat products while, E. faecium and E. mundtii were found to be ruling in case of fruits and vegetables. HPLC analysis was carried out for determination of levels of tyramine. 211 i.e. 86.8% of samples were containing about 1000-1500 mg/l of tyramine, while 10 i.e. 4.1% were found to have tyramine in the range 100-500 mg/l. Negative samples showed 0 mg/l of tyramine.

In early diagnosed and causally treated cases of secondary glaucoma, it is possible to save the sight in a glaucomatous eye and to preserve the sight in a healthy eye. The most common causes of secondary glaucoma include anterior uveitis, in which diverse pathological processes can lead to an increase in IOP. In cases of treating glaucoma secondary to uveitis with annular posterior synechiae in connection with peripheral anterior synechiae, iridencleisis is the method of choice. This article presents the results of the eight-month treatment of such a case with iridencleisis combined with posterior sclerectomy. This method makes it possible to increase the area of aqueous humour outflow to the subconjunctival space. The treatment resulted in the reduction of IOP in the left eye from the preoperative value of 72 mmHg to 15 mmHg, which was maintained at a constant level during the eight-month period following the surgery. Sight assessment in the operated eye, carried out on the basis of PLR, menace and dazzle response, brought a moderately positive result.

An incubation experiment with addition of EDTA and alfalfa into soils contaminated with heavy metal over 200 years was carried out in order to evaluate the EDTA effects on microbial properties. Alfalfa was added to soils together with EDTA to examine its abilities to improve microbial activities affected by EDTA. The obtained results showed that the addition of EDTA led to a significant decrease of microbial biomass C during the first 24 days of incubation. At the end of the experiment the microbial biomass C significantly increased quite close to the original level. The EDTA amendment caused, probably due to the toxic effects, a significant increase in respiratory activities and of the metabolic quotient qCO₂. An addition of alfalfa significantly improved the microbial biomass C contents in arable soils treated together with EDTA. Both, respiratory activities and qCO₂ significantly increased after the soil treatment with EDTA together with alfalfa. EDTA alone decreased the microbial biomass, alfalfa alone as organic substrate was mineralised and utilised by soil microorganisms for their metabolism.

The article describes the competitiveness of Slovak agricultural and food products after the accession of the Slovak Republic into the EU. The analysis has shown that even despite the increased subsidization and full liberalisation of trade, the competitiveness of our products still needs to develop. The main reason behind this is the poor efficiency of production and the lack of innovation activities. Most agricultural commodities are profitable only because of subsidies. Processing industry is the main contributor to the worsening deficit of trade balance. Specific commodities with good sales potential in the EU market include cereals, malt, wheat flour, dairy products, live cattle and sheep meat.

Different transformations of RGB colour space were compared to develop the best method for discrimination of vegetation from the background in open pure cork oak stands in southern Portugal in high-resolution colour imagery. Normalised difference index, i1i2i3 colour space and other indices developed for classic band imagery were recalculated for near infrared imagery and tested. A new method for fully automated thresholding was developed and tested. The newly developed index shows the equal accuracy performance but provides the smallest overestimation error and retains the largest scale of grey levels for a subsequent shape analysis.

In order to determine the environment carrying capacity in terms of biomass production utilizable by game 360 representative sample plots (1 × 1 m) were laid out in the growing season on the basis of typological classification in forest stand of an area of 1,796.49 ha in the studied region of the Soutok Game Preserve, Židlochovice Forest Enterprise, Lanžhot Forest District. On meadows, pastures and others areas producing grass and herbs of a total area of 532.87 ha, other 57 sample plots were laid out and sample of biomass utilizable by game. Quantification of the biomass was carried out on the basis of the area cover of grass and woody undergrowth. In total, forest and non-forest land provides 14,659,851 kg grass and herb utilizable biomass. In forest stands production was found of 6,826,662 kg grass and herb biomass (on average 380 g/m²) and on meadows and pastures 7,833,189 kg (on average 1,470 g/m²). Moreover, production of 1,401,262 kg (on average 78 g/m²). Laboratory analyses were carried out of naturally dried-up samples of biomass and these values available energy were ascertained: the energy of grass and herb biomass amounted to 5.7 MJ/kg, the utilizable energy of wooody origin amounted to 4.03 MJ/kg. In view of the standardized game population size up the available food supply sufficient, because the energy requirement was fully covered by their daily quantitative consumption of biomass.

The quality of water has to be controlled and monitored by drinking water suppliers during all stages of the treatment process from the water sources to the end of distribution systems. The research, performed in Czech Republic from 2006 to 2008, deals with the assessment of the affect of water tanks on the quality of water supplied to consumers, specifically from various points of view: microbiological, biological and physic-chemical changes in water accumulation. Also studied was the influence of the air on the quality of accumulated water (secondary contamination), the influence of the structural layout and hydraulic ratios. In the project quick screening methods (paddle testers and BART^TM tests) were applied in the collection of water samples and scrapings from wetted surfaces of water tanks. The results of the contamination degree discovered in the course of the project solution will serve as basic data for a scale that should evaluate the degree of water tank pollution as well as for resulting corrective measures or optimisation of water tank cleaning. The recommendations of limits for a scraping sample are based especially on the microbiological parameters. Secondary air contamination plays an important role in maintains of biologically stable water. Based on the number of microbial contamination discovered water tanks will be categorised and methods of suitable measures to be taken will be stipulated, operation optimisation as well as cleaning (schedule, methods and frequency of cleaning). The water quality in a storage tanks depends on their maintenance, e.g., to prevent the plaster falling on water surface, the use of antifungal surface coatings (prevention the growth of fungi on walls), the use of ceramics surface of reservoir walls, dark conditions (no windows or blue sheets) in all technological units, the prevention of dust fall out, the selection of suitable air condition and special air filters.

: At present, the coppice-with-standards system has become so rare in floodplain forests that it is considered a natural monument. In 1990, the coppice-with-standards system was not recorded in the territory of the Czech Republic. This state contradicts the increasing interest of nature protection organizations in the relict remainders of the coppice-with-standards system, which is to be considered the closest to naturally preserved lowland forest type, and is, therefore, recommended as the final state of the biocentres and biocorridors in today's floodplain forests. The aim of this paper is to present the results of the inventory dealing with the present occurrence of the coppice-with-standards system in the floodplain forest of the Litovelské Pomoraví Protected Landscape Area (PLA) in a historical context.

Proteolysis is the principal and most complex biochemical event occurring during the maturation of the majority of ripened cheese varieties. In addition to softening the cheese body, proteolysis influences the development of cheese flavour via the formation of amino acid and peptides which make a direct contribution to flavour. Goat, cow and sheep cheeses have been elaborated with raw milk and calf rennet. The extent of proteolysis was monitored over six months of ripening and means of HPLC peptide profile analysis. The influence of season on the changes in hydrophobic and hydrophilic peptides and the HO/HI ratio during the ripening of the cheeses were studied.

Total benefit effect of beer is a result of many individual contributions of natural substances present in such complicated biological material. Recently, regional mark "Czech beer" was obtained by EC. This beer is processed by traditional technology using only recommended brewery materials. Presented work is focused on proteomic and metabolomic analysis of some characteristic active substances specific for "Czech beer". Eight beer samples obtained from retail chain and/or from breweries were enrolled into this study. Polypeptides and proteins that influence beer sensory properties originated mainly from malt. The results of our preliminary study indicate that the main protein fractions in most of beers are protein Z, LTP1 and hordein/glutelin fragments. 2D analyses of "Czech beer" differed in several spots when compared with beer made by other technology. Further, individual beer phenolics originated from malt and hop were analysed by HPLC/UV-VIS and LC/MS. Individual beers differed in distribution as well as in content of phenolics. Hence, some phenolic derivatives seem to be useful as potential authenticity markers.

A collection of lactic acid bacteria (38 Enterococcus and 41 Lactobacillus strains) was tested for the antilisterial activity against 15 Listeria spp. strains (two L. monocytogenes, one L. ivanovii and 12 L. innocua strains) using agar spot method. Out of all 79 bacteria only six Enterococcus strains (1/3A, 3/3A, 6/4D, 6/1A, 1282 and EN3) exhibited antilisterial activity against almost all used indicator strains, when their live cells were tested. When their cell free neutralised supernatants (CFNS) were tested against four selected indicator strains (L. innocua Ln-03, Ln-06, Ln-10 and L. monocytogenes CCM5576) only two Enterococcus spp. strains were active - E. faecalis 6/1A strain from raw cow milk of minor interest due to the activity of its CFNS only against L. innocua Ln-06 and thermolability of the compound and E. mundtii 1282 strain from goat raw milk with CFNS active against 13 Listeria spp. strains including L. monocytogenes. E. mundtii 1282 strain produced probably a bacteriocin, because it completely lost the activity after treatment CFNS with proteinase K.

Hardfacing is one of the most useful and economical ways to improve the performance of components submitted to severe wear conditions. This study has been made for the comparison of microstructure and abrasion resistance of hardfacing alloys reinforced with chromium carbides or complex carbides. The hardfacing alloys were deposited onto ČNS EN S235JR low carbon steel plates by the gas metal arc welding (GMAW) method. Different commercial hardfacing electrodes were applied to investigate the effect of abrasive particle size on abrasive wear resistance. The abrasion tests were made using the two-body abrasion test according to ČSN 01 5084 standard, abrasive cloths were of grits 80, 120, 240, and 400. Microstructure characterisation and surface analysis were made using optical and scanning electron microscopy. The results show the different influence of abrasive particles size on the wear rate for different structures of Fe-Cr-C system. The structures without primary carbides are of high abrasive wear rate, which increases nonlinearly with the increasing abrasive particle size. On the contrary, the structures containing primary carbides are of low abrasive rates and theses rates increase linearly with the increasing abrasive particle size.

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of F_ST (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.

Soils were sampled from the plots with four variants of fertilisation: Nil - without fertilisation, NPK - mineral fertilisation, FYM - farmyard manure, FYM + NPK - farmyard manure with mineral fertilisation, and two variants of crop rotation: field IV - classical 9-year crop rotation, field B - 2-year rotation of alternative growing. Determination of urease, CFU of Azotobacter spp. and potential nitrogenase activity was conducted during the period 1999-2004. The urease activity was positively affected by manure fertilisation (FYM) and by the combination of FYM + NPK. The statistically significantly highest counts of Azotobacter spp. and the highest nitrogenase activity were determined on field B in variants FYM and FYM + NPK. The results show that there was a higher amount of accessible nitrogen present on field IV than on field B. This might explain the lower counts of Azotobacter spp. and therefore the lower nitrogenase activity. According to our results, activity of urease, CFU of Azotobacter spp. and potential nitrogenase activity are very closely connected with N inputs.

The aim of this paper is to show some empirical evidence of the sustainability and decision making in dairy production in Turkey. The main material of the study was obtained from the data collected by the survey conducted in the villages in Merkez, Manavgat and Serik districts of the Antalya province. Within the context of the research, 75 farmers were interviewed in the 2007 production season. The research results indicate that the households are the most important decision maker in terms of farming decisions. 56.3% of the farmers in the small farms, 61.5% of the farmers in the medium-size farms and 41.2% of the farmers in the large farm noted that they could quit dairy production activity if they received a fixed salary.

Dothistroma needle blight caused by Mycosphaerella pini E. Rostrup was observed for the first time in the Czech Republic on an imported Pinus nigra Arnold in 1999. In 2000, it was also found in the open planting. During three years, it became an important pathogen of pines in the Czech Republic. Its occurrence was noticed in more than 50 localities, above all in the region of Moravia and Silesia and eastern Bohemia. In total, it was found on 10 species of pine (P. nigra Arnold, P. banksiana Lamb., P. contorta Loudon, P. mugo Turra, P. leucodermis Ant., P. sylvestris L., P. cembra L., P. aristata Engelm., P. ponderosa P. et C. Lawson and P. jeffreyi Grev. et Balf.). Also Picea pungens Engelm. was noticed as a host species. In the Czech Republic, Pinus nigra is the most frequent host species of M. pini (80% localities) followed by Pinus mugo (27% localities). On Scots pine P. sylvestris, M. pini was noticed at two localities. The critical period for infection is in the Czech Republic from the second half of May until the end of June. The incubation period lasts about 2-4 months depending on climatic conditions. The first symptoms on the needles infected in the current year appear in August being clearly expressed from September to November.In the CR, Dothistroma needle blight spread probably with infected planting stock obtained from import at the end of the 80s and at the beginning of the 90s.

The author analyzes the changes in the tree species composition of Dentario glandulosae-Fagetum Klika 1927 em. Mat. 1964 in the mountain stands of Bieszczady National Park in 1993-2003. The analysis takes into account the changes over a ten-year period in the number and volume structure of each tree species in each stand layer: young natural regeneration, undergrowth, and mature stand. Additionally, after comparing the results with the natural Carpathian forests' structure, the adaptation level of the present tree species composition to the current climate and soil conditions, as well as probable directions of future changes have been assessed. A ten-year period is too short to find significant changes in stand species structure but it is possible to see some trends: beech share in stands is increasing and fir is decreasing.

Geostatistical analysis of bark beetle activity and its use for the improvement of pest control measures are presented. Data on the volume of salvage timber felling due to bark beetle infestation for the period 2002-2004 were used for the analysis. Research was carried out in the northwestern part of Slovakia. The techniques used were variogram modelling, Turning Bands Simulation and selected techniques of simulation post-processing. The maps indicating the probability that a critical volume of timber felling was exceeded in a particular location were produced. The proposed system may be used effectively for the improvement of pest control measures and development of slow the spread strategies. The benefits and shortcomings of this approach in forest protection are discussed.

Knowledge of the content of heavy metals is important because of their impact on the wine stability or on the health of the consumers. The presence of heavy metals in wines is a consequence of an action of various factors such as conditions of the cultivation and processing of grapes and production, stabilisation or storage of wine. In this work determination of heavy metals (Cu, Fe, Zn and Pb) content in red wines was carried out. Selected wine was Plavac mali, Croatian autochthonous sort, produced by various producers during the three consecutive vintages. The aim of this work was to examine whether the wines produced in different vintage, and by various producers, vary according to the content of heavy metals. For the determination of heavy metals content, the atomic absorption spectrometry (AAS) was used. Content of heavy metals ranged between 0.235-1.122 mg Cu/l, 0.809-6.202 mg Fe/l, 0.266-2.434 mg Zn/l and 0.107-0.3355 mg Pb/l. Data analysis showed statistically significant differences only between producers for iron and copper content.