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Koch method of ornamental trees evaluation is based on interest paid on costs invested in tree-growing up to their maturity stage minus deductions for age, defects and damage. The aim of this paper is to apply the above mentioned method in the economic conditions of the Czech Republic. The paper presents essential economic data generated in accordance with the technological model of tree growing at a permanent site. We also describe the mechanism of the so-called base tree price calculation as a basis for the subtraction of deductions derived from its current condition. The calculated base tree prices are illustratively compared to prices based on the to-date applied calculating techniques and the relevant regulation. We also refer to the method of base price adjustment based on the criterion of landscape tree value. The mechanism of price calculation applied for Koch method testing included two modifications. The first relates to due consideration of growth qualities when calculating development care costs; the other introduces landscaping value indicator as the only base price adjustment to achieve the resulting price, corresponding with the current tree condition.

The paper identifies and analyses the main factors which influenced the wine demand. The average annual wine consumption per capita is the basic factor of the wine demand. The average annual wine consumption, one of the wine demand factors, grew between 2003 and 2007 from 16.3 l to 18.5 l per capita. Following this trend, we can expect the average annual wine consumption 19.0 l per capita in year 2010. A positive feature of this development is also the fact, that there grows the demand after the quality wines. At the same time, there grows also the consumption of the lower quality, cheap wines packed in boxes or PET bottles. A continuously growing tendency can be also noticed in the red-wine consumption. This change of consumer preferences has significantly influenced the growth of the share of the red varieties in the new-planted vineyards. However, there are critical factors in the development of the demand for wine demand here. Specifically, it is the daily feasible ration of alcohol in wine and other alcoholic drinks, especially beer that we can treat as the substitute of wine. The average annual beer consumption moves about 160 l per capita in the Czech Republic. The continuously growing tendency of wine consumption is reduced by the lower price of beer too, because beer is the cheapest alcoholic drink in the Czech Republic. The taste and preferences of consumers have been changing, that means, that the consumers are the ones who decide about the wine quality, and not producers. That is why this paper includes the partial results of the research oriented on the habits and behaviour of consumers in the wine market in the Czech Republic. It is concentrated on the wine sellers opinions on demand development in the wine market.

In the present study, the main purpose was the selection of antagonistic starter cultures for pickle and olive fermentation processes. The chemical and microbiological properties of fermented 70 pickle and 16 olive samples collected from the province of the west part of Turkey were analysed. Subsequently, lactic acid bacteria strains producing bacteriocin-like metabolites were isolated and identified. From 86 samples, 16 isolates were chosen, depending on their partial antimicrobial activity against at least one selected indicator. 13 out of this 16 isolates were identified as Lactobacillus plantarum whereas 3 of them proved to be Lactobacillus pentosus. Moreover, all the relevant isolates were found to be potent acid producers. All these results obviously suggest that the isolated Lactobacillus plantarum 9 and 25 were appropriate for them to be proposed as starter cultures in fermented pickle and olive production.

The mycological conditions on study plots established in forests growing on former agricultural farm lands were studied. In young spruce stand (8-10 years) reduced and unstable spectrum of macromycetes was found. After approximately 50 years of forest growth the situation became stable and spectrum of macromycetes together with development of mycorrhizal status were similar to a situation found in stands on forest soils. Slightly increased occurrence of saproparasitic species of fungi (e.g. Heterobasidion annosum at others) was observed in older growths.

Within the framework of the research project ELISA methods for the quantitative determination of allergenic substances in foodstuff and raw materials were developed. ELISA kits for allergenic proteins of milk (casein, beta-lactoglobulin and BSA) egg white proteins and mustard proteins were validated and collaborative studies were performed to prove the validation of the ELISA methods developed. Various methods of extraction were tested. The parameters as a limit of detection, as a limit of quantification, robustness, repeatability and accuracy were determined. A broad range of zero matrices for allergens were tested as well. The ELISA kits are suitable for the determination of allergens according to EU legislation Directive 2005/26/EC and Directive 2006/142/EC in the laboratories focused on this topic.

In early diagnosed and causally treated cases of secondary glaucoma, it is possible to save the sight in a glaucomatous eye and to preserve the sight in a healthy eye. The most common causes of secondary glaucoma include anterior uveitis, in which diverse pathological processes can lead to an increase in IOP. In cases of treating glaucoma secondary to uveitis with annular posterior synechiae in connection with peripheral anterior synechiae, iridencleisis is the method of choice. This article presents the results of the eight-month treatment of such a case with iridencleisis combined with posterior sclerectomy. This method makes it possible to increase the area of aqueous humour outflow to the subconjunctival space. The treatment resulted in the reduction of IOP in the left eye from the preoperative value of 72 mmHg to 15 mmHg, which was maintained at a constant level during the eight-month period following the surgery. Sight assessment in the operated eye, carried out on the basis of PLR, menace and dazzle response, brought a moderately positive result.

Forty samples of grass silages, made from the five most widely used grass species in ruminant nutrition (Dactylis glomerata L.,Phleum pratense L., Lolium perenne L., Festuca arundinacea L. and hybrid Felina) were tested in the present study. These grass species conserved by an ensiling process were compared among each other according to dry matter (DM) rumen degradability parameters (a = portion of DM solubilized at initiation of incubation, b = fraction of DM potentially degradable in the rumen, c = rate constant of disappearance of fraction b and ED_DM = effective degradability of DM, estimated for each ingredient assuming the rumen solid outflow rates of 0.02 (ED_DM2), 0.05 (ED_DM5) and 0.08 (ED_DM8) h^-1). Based on the chemical composition of grass silages the regression equations for prediction of ED_DM were evaluated. The influence of the ensiling process on dry matter degradability parameters was also assessed. The best values of ED_DM were determined for Lolium perenne (ED_DM2 = 753.2, ED_DM5 = 631.1 and ED_DM8 = 567.7 g/kg DM). The best predictor was NDF (R²-values of 0.757 (ED_DM2), 0.863 (ED_DM5) and 0.906 (ED_DM8)). Using two predictors the accuracy level increased. The combination of CF and NDF gave R²-values 0.892, 0.920 and 0.929 for ED_DM2, ED_DM5 and ED_DM8, respectively. The regression equations based on the most important grass species harvested in different vegetation periods seem to be a useful tool for practical use. No significant P < 0.05) effect of the ensiling process in relation to dry matter rumen degradability parameters was proved.

This paper addressed the spatial distribution characteristics of organic matter, total nitrogen, extractable phosphorus and extractable potassium in agricultural soils of Northeast China. The related factors were explored using geostatistics and geographic information systems. The results showed that the log-transformed data of the four soil nutrients followed a normal distribution. Soil extractable phosphorus had a higher coefficient of variation. The experimental variogram of the log-transformed data of soil organic matter, total nitrogen and extractable phosphorus was fitted with an exponential model, while soil extractable potassium was fitted to a spherical model. Soil samples from smaller slope gradients had higher organic matter and total nitrogen. Soil type affected the four soil nutrients significantly. Soil samples from dry farming land had significantly higher total nitrogen and extractable potassium than soil from paddy fields, while the contrary was found for extractable phosphorus. Along the Yinma River, soil samples from the western part have statistically higher values for organic matter, total nitrogen and extractable potassium than those collected from the eastern part.

The contribution deals with the development of livestock since 1990. Since that year, beef and dairy cattle numbers have dropped by more than 50 percent in the Czech Republic. This decrease has been partly compensated by an increase in efficiency, yet milk production has dropped to 55% in this period and beef production to 60% compared with 1989. The declining amount of cultivated land in the Czech Republic has resulted in a decrease in the stocking rate and the corresponding production. It adversely influences the economy namely in highland areas. In this sense the high rate of permanent pastures with low stocking rate enables to efficiently exploit the EU direct payments and thus the direct payment system discriminates above all the farms in highland areas (potato and oat-growing areas and upland production areas).

The article deals with the analysis of food market on the level of consumer prices structure, through the identification of margins in the selected food verticals and through the analysis of consumer behaviour associated with changes in prices and income of population. The article also discusses the factors which may affect the current growing trend in prices of food in the SR and worldwide and subsequently, the reduction of gap between the Slovak and the EU prices.

The paper describes the policy, legislative and organisational context and structure of financing the forestry from public funds in the Czech Republic, Estonia, Poland and Slovenia in the 90's. The first half of the nineties was the period of the most significant changes, whereas more stable and systematic financing of forestry was generally started in the second half of the nineties. The supported activities reflected main problems and challenges facing the forestry in individual countries. In the Czech Republic the substantial support was designed for protective measures and restitution of damaged stands. In Poland, the increase of forest area and restitution of forest stands damaged by air-pollution were the priority. Slovenian budget supported mainly the improvement of road infrastructure, forest management planning, as well as sustainable utilisation of forests through marking of trees for cutting. Estonian forestry received the relatively lowest subsidies allocated mainly to the preparation of management plans and extension services. Subsidies were the most important instruments of support. In Poland, however, tax exemptions and concessions were significantly higher than the value of financial means allocated from the public funds.

The influence of the date of cutting collection and cutting position in the crown on rooting quality was evaluated in cuttings taken from seven-year ortets. The evaluation of various dates of cutting collection in spring demonstrated a possibility of successful propagation by cuttings during a relatively long period from full bud dormancy to flushing onset. The relationship between the development of aboveground parts (flushing, shoot and bud formation) and rooting quality was not established. The exposure of cuttings in the crown of parent tree with respect to the cardinal points did not influence rooting percentage and quality. A somewhat higher rooting ability was observed in cuttings from lower parts of the crown in comparison with cuttings taken from the highest whorls. Differences were more perceivable in generally weak-rooting clones. The comparison of rooting quality in cuttings from seven- and eight-year mother plantations with cuttings from a sixteen-year mother plantation confirmed that not only the rooting of cuttings collected from older mother plantations is weaker but also their growth is slower and their plagiotropic growth continues for a longer time.

Hardfacing is one of the most useful and economical ways to improve the performance of components submitted to severe wear conditions. This study has been made for the comparison of microstructure and abrasion resistance of hardfacing alloys reinforced with chromium carbides or complex carbides. The hardfacing alloys were deposited onto ČNS EN S235JR low carbon steel plates by the gas metal arc welding (GMAW) method. Different commercial hardfacing electrodes were applied to investigate the effect of abrasive particle size on abrasive wear resistance. The abrasion tests were made using the two-body abrasion test according to ČSN 01 5084 standard, abrasive cloths were of grits 80, 120, 240, and 400. Microstructure characterisation and surface analysis were made using optical and scanning electron microscopy. The results show the different influence of abrasive particles size on the wear rate for different structures of Fe-Cr-C system. The structures without primary carbides are of high abrasive wear rate, which increases nonlinearly with the increasing abrasive particle size. On the contrary, the structures containing primary carbides are of low abrasive rates and theses rates increase linearly with the increasing abrasive particle size.

Unit hydrograph is a basic method to show changes in runoff in the watershed. The investigation of runoff changes was carried out in the U Dvou louček watershed situated at the summit part of the Orlické hory Mts., East Bohemia. The waveform ordinates of recession limbs of unit hydrographs obtained using a common approach had to be approximated by the least-squares method. Final hydrographs reflected both drainage treatment and forest stand growth influencing the runoff from the watershed. Both factors increase culmination in synergy and reduce runoff on the recession limb of the hydrograph. We confirmed increased maximum runoff taking up 25-30% of the total runoff time when waterlogged sites were drained. The culmination increased by 0.2-0.8 mm/hour indicates the runoff increased by 2-8 m³/ha/hr.

The study is a follow-up of the production potential of Douglas fir in mesotrophic sites of the Křtiny Training Forest Enterprise (TFE) (Journal of Forest Science, No. 7, 2008). Production parameters (height, dbh, volume) of Douglas fir are also evaluated, but in acid sites of the Hůrky Training Forest District, Secondary Forestry School in Písek in mature stands. In total, 17 mixed stands with the proportion of Douglas fir aged 88 to 121 years were assessed. Comparing 10 Douglas fir trees with 10 Norway spruce, Scots pine or European larch trees of the largest volume, higher and generally markedly higher production potential of the introduced Douglas fir was always found in all assessed stands. Groups where the volume of Douglas fir trees was two to three times higher than the volume of spruce, pine or larch were not an exception. For example, in stand 22B10, the mean volume of the 10 largest Douglas fir trees was 6.30 m³ but the volume of spruce trees was only 1.93 m³ and the volume of larch trees 2.25 m³. Differences between the mensurational parameters of Douglas fir and spruce (or larch) assessed by the ANOVA test were statistically highly significant. At present (based on annual ring analyses), the volume increment of particular Douglas fir trees ranges at level of 0.06 to 0.10 m³/year (i.e. about 0.6 m³ to 1.0 m³ per 10 years) in mature stands.

The study objectives were to test ready-to-eat food from the retail market in the Czech Republic for the presence of L. monocytogenes and, based on typing methods, to investigate probable causes of contamination. A total 2180 samples of ready-to-eat food (meat, dairy, fish, delicatessen and confectionery products and fresh fruit and vegetables) were analysed qualitatively and quantitatively. L. monocytogenes isolates were characterised by serotyping and macrorestriction analysis after digestion with the restriction enzyme AscI. In 2004-2008 L. monocytogenes was most often detected in delicatessen (5.2%), meat (3.4%) and dairy products (1.8%). In the analysed samples, L. monocytogenes was mostly present at counts lower than 102 CFU/g. Only in 2004, higher counts of L. monocytogenes were found in two heat-processed meat products (103 CFU/g). The obtained macrorestriction patterns helped in tracing the source of contamination and routes of the spread of L. monocytogenes in the manufacturing plant and retail market.

: In chick hatcheries, males of laying hybrids are considered to be "waste" and the majority of these males are killed just after hatching. On the other hand, the interest of consumers in products from alternative systems (organic, free-range) is increasing. The idea was to evaluate the meat quality of these males when they have access to free range because there is not such a study available. The aim of this study was to compare the physical and sensory quality of the meat of layer males with fast-growing broilers at the same age when they had both access to free range and when they were fed to 49 and 90 days of age. Slow-growing ISABROWN (IB) layer males and fast-growing Ross 308 (RS) chickens were kept in free-range conditions to evaluate carcass and meat quality at 49 days and 90 days of age. Live weight, carcass yield, breast meat yield and the proportion of abdominal fat were significantly higher (P < 0.001) in RS at both ages. The proportions of fat in the breast meat were significantly lower (P < 0.01) in IB at both ages. The value of pH 24 h was significantly higher in IB and the meat was darker (P < 0.001) in these chickens. The overall acceptability was significantly better (P < 0.01) in IB at 90 days of age. The laying males are acceptable for an alternative system of poultry meat production from the aspect of meat quality. The quality was comparable or even higher in comparison with fast-growing chickens.

An incubation experiment with addition of EDTA and alfalfa into soils contaminated with heavy metal over 200 years was carried out in order to evaluate the EDTA effects on microbial properties. Alfalfa was added to soils together with EDTA to examine its abilities to improve microbial activities affected by EDTA. The obtained results showed that the addition of EDTA led to a significant decrease of microbial biomass C during the first 24 days of incubation. At the end of the experiment the microbial biomass C significantly increased quite close to the original level. The EDTA amendment caused, probably due to the toxic effects, a significant increase in respiratory activities and of the metabolic quotient qCO₂. An addition of alfalfa significantly improved the microbial biomass C contents in arable soils treated together with EDTA. Both, respiratory activities and qCO₂ significantly increased after the soil treatment with EDTA together with alfalfa. EDTA alone decreased the microbial biomass, alfalfa alone as organic substrate was mineralised and utilised by soil microorganisms for their metabolism.

In the years 2005, 2006, and 2007 the described pomological characteristics were evaluated and both heritability and correlation coefficients of the determined characteristics of parents and progeny were estimated for the Minaret × Betinka apricot hybrid population. Estimated heritability of fruit weight, over colour and flesh consistency was approximately 0.9. Heritability of flesh colour, fruit attractiveness and fruit flavour ranged from 0.6 to 0.9. The correlations show a strong dependence of the following characteristics of parent varieties and progeny: over colour and fruit attractiveness. Medium strong dependence was determined in the following characteristics: fruit colour and fruit flavour. Weak dependence was found in the case of fruit size.

The effect of thinning on the structure and accumulation of litterfall and holorganic horizons (L, F, H) in young Norway spruce stands was investigated. The research was conducted on a Norway spruce thinning experiment Polom (established in 1980) in the Orlické hory Mts. (north-eastern part of the Czech Republic). In 1992, the monitoring of litterfall started on an unthinned control stand (plot 1) and on a comparative stand with very heavy thinning from below (plot 3). During the period of observation (age of the stand 27-37 years), the total weight of litterfall ranged between 1,800 and 4,800 kg/ha. The amount of litterfall was partly influenced by climatic factors (precipitation and temperature), growth processes (basal area increment) and thinning regimes in individual years. The results of the investigations showed that dry biomass accumulated under a 36 years old Norway spruce stand ranged from 9,200 to 11,300 kg/ha in horizon L, from 37,000 to 38,200 kg/ha in horizon F and from 138,300 to 146,400 kg/ha in horizon H. The quality (content of basic nutrients) of litterfall and material from holorganic horizons are discussed.

Soil properties of forest ecosystems depend on synergy of both parent material and organisms living in the soil, i.e. tree species communities including related plant and animal species. However these soils were not left intact being converted into agricultural land; addition of both nutrients and organic matter and cultivation using tillage led to increased fertility of topsoil. Even long-term afforested soils show differences which are considered as legacy of past agriculture. The change remains detectable for decades; though the altered properties are obvious especially couple of years after planting (approximately 10 years). We found increased concentrations of nutrients (P, K, Ca, and Mg) and subsequent increased base saturation (V %) in former tilled soil only. Moreover, there were no differences between topsoil and subsoil properties (69% and 72%, respectively). In addition to significantly lower saturation (both 0-10 cm and 11-30 cm layers) detected in the long-term-forest and 50-year-afforested (both covered with Norway spruce stands) soils in comparison with adjacent 10-year-old afforestations, there was found significantly lower base saturation in topsoil horizons compared to underlying ones.

Furan and its derivatives were identified in a small number of heat-treated foods back in the 60's and 70's. In May 2004, US Food and Drug Administration published a report on the occurrence of parent furan in a number of thermally treated foods. Since furan has been classified as "possibly carcinogenic to human" by IARC, a great concern has been addressed to the analysis of this substance naturally-occurring in food. This paper gives a short overview on the mechanistic pathways of the parent furan formation in food by degradation of amino acids and/or reducing sugars, and oxidation of ascorbic acid and poly-unsaturated acids which can be induced by thermal or irradiation treatments; further, it deals with the metabolism and toxicology of furan as well as with the comparison of the methods of furan determination.

The content of arsenic was determined in the rainbow trout muscle (Oncorhynchus mykiss) and in rice samples. The method of atomic absorption spectrometry with hydride generation (HG-AAS) in graphite furnace was used for total arsenic determination. Arsenic speciation was done using the high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS). The rainbow trout muscle was extracted by ultra pure deionised water; trifluoroacetic acid was used for the extraction of rice samples. Total arsenic values ranged from 60.90 μg/kg to 310.00 μg/kg in rice samples and from 0.72 mg/kg to 2.23 mg/kg in rainbow trout muscle. An inorganic trivalent arsenic As(III) was determined as the main species in rice and non-toxic arsenobetaine in the samples of rainbow trout.

The water regime variability in most catchments is frequently influenced not only by the changes of the vegetation cover in the annual cycle but also by its development in the time span of decades. That means that the resulting evapotranspiration depends not only on the actual climatic situation but also on the soil moisture. The simulations of the rainfall-runoff process have been used with the intention to follow the possible role of the developing land cover. The differences between the observed and simulated flows in relatively long periods can be considered as an appropriate tool for the assessment of the water regime changes, in which the evapotranspiration demand variability is a significant phenomenon.

The analysis comprised purebred Charolais bull calves (CH) and crossbred bull calves born from Hereford (CH × HE) and Simmental (CH × SI) cows. Body weights and daily gains were measured in subsequent periods of the experiment and the post-slaughter evaluation was carried out. Carcass lean weight and dressing percentage were evaluated, as well as the lean content of five prime cuts. Total weight of the five prime cuts was measured and its share in the half-carcass weight was determined. The rib-eye area was also measured. The CH × SI crossbreds reached significantly higher body weights at weaning, 210-day body weights and weights at finishing, as well as significantly higher daily gains in all the periods (P < 0.05, P < 0.01) and were characterized by the heaviest carcasses and most cuts, particularly the round. The crossbreds had the highest total weight of five prime cuts and total lean weight, highest weight of the round and lean weight in the round. Other relationships were found analyzing the share of each cut in the carcass. The share of tenderloin and rump in the carcass of CH × SI was significantly lower compared to the purebred bulls (P < 0.01) and CH × HE (P < 0.05). They also had a significantly lower share of the shoulder, shank and shin, as compared to the other groups, as well as a significantly higher (P < 0.05) share of the neck, as compared to the purebred bulls. The purebred bulls also achieved good results and had the highest share of the prime cuts and the largest mean rib-eye area.

Gas chromatography and IDF Standard method (1999) were used to analyze the fatty acid composition of milk fat of mares originating from the following breeds: 10 of Wielkopolska breed, 10 Konik Polski Horses, and 9 Polish Cold-blooded Horses. Eighty-seven mare's milk samples were collected in the years 2000-2002. Unsaturated fatty acids were shown to prevail in the milk fat of mares of Wielkopolska breed (61.32%) and of Konik Polski mares (52.58%) whereas saturated acids prevailed in the milk fat of Cold Blooded mares (54.95%). The study revealed that the fatty acid composition of the investigated groups of mares was breed-specific. Of course, the impact of other uncontrolled factors such as nutrition is not excluded, either.

Soils were sampled from the plots with four variants of fertilisation: Nil - without fertilisation, NPK - mineral fertilisation, FYM - farmyard manure, FYM + NPK - farmyard manure with mineral fertilisation, and two variants of crop rotation: field IV - classical 9-year crop rotation, field B - 2-year rotation of alternative growing. Determination of urease, CFU of Azotobacter spp. and potential nitrogenase activity was conducted during the period 1999-2004. The urease activity was positively affected by manure fertilisation (FYM) and by the combination of FYM + NPK. The statistically significantly highest counts of Azotobacter spp. and the highest nitrogenase activity were determined on field B in variants FYM and FYM + NPK. The results show that there was a higher amount of accessible nitrogen present on field IV than on field B. This might explain the lower counts of Azotobacter spp. and therefore the lower nitrogenase activity. According to our results, activity of urease, CFU of Azotobacter spp. and potential nitrogenase activity are very closely connected with N inputs.

Enterotoxigenic Bacillus cereus was detected in a variety of meat stuffs (36), ready-to-cook products (5), and swabs (7). The bacterial colonies isolated from PEMBA agar were identified as B. cereus. The 85 isolates were examined for the enterotoxin production using both TECRA-VIA and BCET-RPLA kits (ELISA - 47, RPLA - 38). Thirty two isolates (66%) were positive for enterotoxin using the ELISA test while only 15 isolates (39%) gave positive results in the RPLA test system. In total, 178 (91.8%) and 164 (84%) of the strains isolated in our laboratory (from various foods) were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Parallel enterotoxin positive results obtained using both tests were demonstrated in only 9 isolates from 19 assessed (47.4%). Coincidental negative results from both kits were established for 3 isolates (15.8%) only. The isolates of B.cereus from meat were resistant to cephalothin (57%), clindamycin (14%), oxytetracycline (14%), and erythromycin (7%). The isolates from swabs were resistant to cephalothin (83%), erythromycin (16%), clindamycin (16%) and enrofloxacin (16%).