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This paper analyzes the data on the development of the main factors of the demand for wine and wine consumption in the Czech Republic. The average annual wine consumption, one of the wine demand factors, grew between1991 and 2005 from 14.8 l to 17 l per capita. Following this trend, we can expect the average annual wine consumption 17.4 l per capita in year 2010. However there are critical factors in the development of the demand for wine demand here. Specifically it is the daily feasible ratio of alcohol in wine and other alcoholic drinks, especially beer that we can treat as the substitute of wine. The ethanol consumption in beer accounts for 50% of the total ethanol consumption in alcoholic drinks in the Czech Republic. The negative influence on wine demand growth has been the price of other alcoholic drinks, especially beer that is the cheapest form of ethanol.

In this paper, the authors summarise the land use changes in the upper reaches of the Krupá river catchment, which is a left tributary of the Morava River. During last 70 years, the catchment was exposed to many important historical events that have been inscribed in the physique of the landscape in a very interesting way. The land use changes, which occurred during the last eight decades in the subcatchment of the Krupá river basin, have been analysed using historical maps, cadastral maps, and both historical and recent aerial photographs of the area. The next step is to estimate, through the CN method and DesQ hydrological model, how the runoff processes in the Krupá River catchment could be influenced by the land use changes.

Physical, chemical and microbiological properties of soils were studied in black walnut (Juglans nigra L.) stands and compared with those in stands of natural species composition, in mixed stands of black walnut with linden and in pure oak stands. The objectives were to consider a possibility of black walnut planting at floodplain sites in the alluvial area of Southern Moravia. The first results did not show any worsening of soil properties and soil production potential. A positive amelioration effect of soil- improving species was proved in black walnut stands.

This report describes a clinical case and development of the polyglandular failure syndrome including hypothyroidism and hypoadrenocorticism in a 6-year-old female Black Russian Terrier. The bitch was presented because of hypothyroidism on the first occasion. Oral supplementation with sodium levothyroxine resulted in clinical improvement. Four months later, it was presented again with similar and rather vague clinical signs and diagnosed with adrenocortical deficiency both in mineralocorticoid and glucocorticoid functions. Indirect immunofluorescence to investigate the presence of circulating autoantibodies against the thyroid and adrenal glands revealed only anti-thyroid antibodies (microsomal pattern) at a dilution of 1:40. Since then, the bitch has been on hydrocortisone and fludrocortisone with no adjustments necessary for a year. The combination of two endocrinopathies and the episodic course of hypoadrenocorticism made the final diagnosis difficult, posed a diagnostic challenge and required the owner's compliance with diagnostic and therapeutic procedures.

The present paper deals with derivation of target stocking in forests of Norway spruce vegetation zone. Target stocking in forests with prevailing ecological and social functions is the stocking when the forest fulfils demanded functions in the best way. For forests in the Norway spruce vegetation zone target stocking was derived by original procedures as an optimum stocking in harmonization of demands on the fulfilment of ecological functions (especially erosion control, hydrological and water protection ones), securing static stability and preconditions for the formation and growth of natural regeneration. We investigated the relations between stocking and indicators of static stability (slenderness coefficient and ratio of crown length to tree height), natural regeneration phases, ground and non-wood vegetation coverage and natural regeneration coverage. The most favourable status of these indicators was found out in stocking 0.7 and in the upper forest limit 0.6.

Damage caused by bark peeling and browsing by red deer to Picea abies was investigated in two localities in the Českomoravská vrchovina Highlands. 127 experimental plots of 50 × 50 m in size were monitored in stands aged from 15 to 93 years. In total, 44% of 16,700 inspected trees were damaged by bark peeling and browsing. The area of gaping wounds varied from 50 to 1,650 cm². Stem decay affected 68% of the damaged trees. Some properties of the decay were analysed from 200 sample trees felled on the plots. Bark peeling damage occurred when the trees were 4 to 48 years old. Decayed wood accounted for 22-70% (mean 42%) of the merchantable stem volume of sample trees. The mean rate of the vertical decay spread was 17.4 cm per year (ranging from 4-63 cm per year). Mean decay volume correlated positively and mean spreading rate correlated negatively with the time elapsed from the bark peeling damage occurrence.

This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium avium subsp. hominissuis was diagnosed in two sibling Fjord horses. In the first horse, M. a. hominissuis was detected by qPCR in numbers of 2.89 × 10⁵ and 1.47 × 10⁴ cells per 1 g of intestinal content and mesenteric lymph nodes, respectively; in the second horse, faeces and mesenteric lymph node samples showed numbers of 6.31 × 10⁵ and 3.36 × 10⁶ cells per 1 g of tissue, respectively. Another aim of this study was to comprehensively describe clinical and pathological findings in both animals.

Carbon and nitrogen transformations in soil are microbially mediated processes that are functionally related. The fate of C and N was monitored in a clay-textured soil (Typic Haplocambid) which was either unamended (control) or amended with various plant materials at the rate of 10 g residue C/kg soil. To evaluate C mineralization, soils were incubated for 46 days under aerobic conditions. Nitrogen mineralization/immobilization was evaluated at the end of eight-week incubation experiment. All CO₂ evolution data conformed well to a first-order kinetic model, C_m = C₀ (1 - e^-Kt). The product of K and C₀ (KC₀) was significantly correlated with some chemical and biochemical properties of the plant residues, including N concentration (r = 0.83, P < 0.001), C:N (r = -0.64, P < 0.05) and lignin:N (r = -0.81, P < 0.001). Among the plant residue composition characteristics, N concentration (r = 0.96, P < 0.001), C:N (r = -0.69, P < 0.01) and lignin:N (r = -0.68, P < 0.01) were significantly correlated with the net rates of N mineralization/immobilization (N_m/i).

The effect of three seedling treatments: T₀, normal germination; T₁, cotyledons removed; T₂, cotyledons removed 5 days earlier than in T₁; and two phosphorus levels (P₀ and P₃₀) on nodulation and nodule function in soybean [Glycine max (L.) Merr.] were investigated in nutrient solution culture. The number of nodules formed at P₀ was in the order T₂ > T₀ > T₁, but it was T₀ > T₂ > T₁ at P₃₀. Nodule dry weight per plant had the same tendency as the nodule number. Nodule size (dry weight per nodule) in seedlings ranged from 0.601 to 1.089 mg in the order T₀ > T₁ > T₂, regardless of P level. For example, nodule size in T₀ was larger by 86% and 52% than T₂ at P₀ and P₃₀, respectively. Furthermore, regardless of P level, a specific acetylene reduction activity (ARA, µM C₂H₄/h/g nodule) increased with P content in seedlings, but no significant difference was found (P < 0.05). Leghemoglobin (Lb) content was not significantly affected by P level; however, seedlings (T₀ and T₁) significantly affected the Lb content per unit plant biomass (P < 0.05). All these results suggest that seedling P content plays a key role in nodulation and nodule function of soybean.

The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied on plant cell lines. When plant cell suspension culture of Nicotiana tabacum BY-2 was treated with cisplatin a decrease of viability with its increasing concentration was observed as well as the mitotic index. The structure of nuclei has also been changed in dependence on cisplatin concentration.

Experiments were conducted on calves divided into three groups, 6 animals in each, to determine the influence of the intake of non-acidified and acidified milk replacer on the level of acid-base homeostasis in venous blood. The milk replacer was supplied at a dose of 700 g dry matter in 8 l of the liquid in two feedings. The milk replacer was acidified by adding formic acid to ensure the concentration of 0.2% in milk replacer. Venous blood was collected before feeding and 1, 2, 4, and 6 hours after feeding. The samples were analyzed for blood pH, actual bicarbonate HCO₃^- (mmol/l), base excess BE (mmol/l), partial pressure of carbon dioxide pCO₂ (kPa) and partial pressure of oxygen pO₂ (kPa). Significant changes were observed in calves fed acidified milk of albumin type. The mean daily values of acid-base parameters in these calves were significantly lower in comparison with animals fed non-acidified milk replacer and reached the following levels: pH 7.343 ± 0.032 (P < 0.05), HCO₃ 24.49 ± 2.13 mmol/l (P < 0.01), BE 1.11 ± 1.97 mmol/l (P < 0.001). A similar tendency but of a more pronounced decrease in values was recorded in the group of calves fed acidified milk replacer of casein type: pH 7.312 ± 0.022 (P < 0.01), HCO₃^- 21.73 ± 0.75 mmol/l (P < 0.001), BE 96 ± 0.86 mmol/l (P < 0.001). In relation to the time after feeding the group of calves fed non-acidified milk replacer showed a rising tendency in the level of metabolic components (HCO₃^-), compensated by respiratory regulating mechanisms (rise in pCO₂) conducive to the maintenance of optimum blood pH level. The group of calves fed acidified milk replacer (formic acid 2 ml/l) of the albumin type showed metabolic acidosis with subsequent gradual adjustment and compensation by means of metabolic (HCOHCO₃^-) rather than respiratory regulation mechanisms. The calves fed acidified casein type milk replacer displayed metabolic acidosis with insufficient metabolic regulation and more intensive respiratory compensation (decrease in pCO₂). Concurrent investigations of the abomasum acidity and blood acid-base homeostasis reflected the joint action of both the acidifying effect of formic acid and significantly lower production of bicarbonate (HCO₃^-) related to the intake of acidified milk and the tendency to the development of metabolic acidosis.

The Velvet Revolution in November 1989 in the former Czechoslovakia opened the way to the renewal of the democratic political system. One of the most visible aspects of the Czech political development consisted in the renewal of the essential functions of elections and political parties. On the local level, however, the political process - as well as in other post-communist countries - continued to be for a long time influenced by the remains of the former centralized system wherein the local administration used to be subjected to the central state power. Municipal elections took hold in these countries, however, the local government remained in the embryonic state and a certain absence of real political and economic decision-making mechanism on the local level continued to show. The public administration in the Czech Republic had to deal with the changes in the administrative division of the state, the split of the Czechoslovak federation as well as the fragmentation of municipalities whose number increased by 50 percent. Decision making mechanisms on the local and regional level were suffering from the incomplete territorial hierarchy of public administration and from the unclear division of power between the state administration and local administration bodies. Only at the end of the 1990s, the public administration in the Czech Republic started to get a more integrated and specific shape. Citizens participation in the political process represents one of the key issues of representative democracy. The contemporary democracy has to face the decrease in voter turnout and the low interest of citizens to assume responsibility within the political process. The spread of democratising process following the fall of the iron curtain should not overshadow the risk of internal weakness of democracy. The solution should be looked for in more responsible citizenship and citizens political participation. The degree of political participation is considered (together with political pluralism) to be the key element of representative democracy in general terms, as well as of democratic process on the local and regional level. The objective of this paper is to describe the specifics of citizens local political participation in the Czech Republic and to show the differences between rural and urban areas. The paper concentrates on voting and voter turnout but deals also with other forms of citizens political participation.

Farm-sale, also known as direct sale, provides major opportunities to farmers in the future. This kind of sale is of increasing popularity in Europe, but farmers have to be familiar with the regulations concerning processing and sales. Mainly small and medium farmers prefer direct sale. In this activity, they must compete with an increasing number of hypermarkets, supermarkets and wholesale markets. When talking about direct sale, it means that farmers sell their products directly to customers. There are more options: (i) sale in their own shop, (ii) through a catalogue and (iii) delivery to restaurants and shops. It has to be mentioned that the development of special local products means the products representing a common local value and principally those that can be associated with a specific village due to their historical heritage or tradition. There is no standard or official definition for special local products that includes all the possible factors. Efforts of marketing and rural development experts are needed to identify and market these special products to the appropriate consumers. Meanwhile it has to be noticed that, mainly in Europe, the definition and the possibilities of product regulation concerning geographical origin, are clearly defined and well-known. However, the "protection of geographical origin" is not the same issue as "special local products" mentioned above. In a wider sense, these can be described from a marketing point of view as "local product, common product" that interconnect and integrate villages, people and approaches, but are not regulated and protected legally. These products mentioned above reach the consumer in relatively small quantities, through direct sale, and they are often attached to the services of rural tourism. The local products are also developed to ensure high quality products for the consumer or to attract tourists. People can be proud of them since they cannot be bought anywhere else. Advisers are also helping farmers choose the right sales channels to diversify their marketing activities.

In the territory of the Czech Republic there are more than 50% of agricultural soils exposed to water erosion; it is a very urgent problem both at present and for the future. It must be solved now when there is still something to be protected. It is rather complicated to describe the soil properties in terms of soil susceptibility to water erosion because it is a complex relation in which many factors participate. For the complex evaluation of all main factors participating in erosion origination it is possible to apply the Universal Soil Loss Equation (USLE). It consists of six factors interacting with each other and participating in the origination of soil erosion. One of these factors is the soil erodibility factor (K-factor), the revision of which for soil conditions of the CR is the subject of this study. In total ca. 5000 soil pits from the whole territory of the country were processed and evaluated in detail. The main results of this study are K-factor values (means and variances) for the soil types, subtypes and varieties (represented in the database) according to the Taxonomic Classification System of Soils of the Czech Republic.

The objective of the study was to evaluate the growth potential of Carpathian birch (Betula carpatica W. et K.) at an environmentally harsh mountain site and a response of this species to altered soil chemistry after dolomitic limestone and basalt grit applications. The Carpathian birch proved to be a suitable species for the replanting of extreme acidic mountain sites. This birch shows a low mortality rate, grows well in the clear-felled patches and soon forms a cover which is necessary for the reintroducing of more sensitive tree species. The application of dolomitic limestone and basalt grit resulted in the slower growth of Carpathian birch plantations. Liming raised soil reaction, sum of exchangeable bases, base saturation, cation exchange capacity and reduced exchangeable Al content. On the other hand, liming decreased an amount of oxidizable soil organic matter and negatively affected soil N, exchangeable P and K. Basalt grit increased exchangeable P and K contents and raised soil reaction, however only slightly. The influence of basalt grit on the sum of exchangeable bases, base saturation and cation exchange capacity was also less pronounced compared to liming. Basalt grit elevated the proportion of exchangeable aluminium and reduced the content of soil N.

The paper summarizes the results of 15-year natural regeneration for beech of five plots with different densities situated in the Western Carpathians Mts. Three of the plots were subjected to differently intensive shelterwood cuttings (plots L, M, H), one plot was clear-cut (CC), and one was left without intervention - as a control (C). The number of one-year-old seedlings decreased proportionally with increasing cutting intensity. The ANOVA results document a significant influence of cutting intensity on the abundance of both one-year-old and older seedlings. The abundance of beech seedlings was initially increasing with increasing cutting intensity, and, having reached the peak on plot M (medium intensity), there followed a decrease in the seedling abundance. Lower numbers of beech seedlings on plots subjected to less intensive cutting (C, L) result from less favourable growth conditions in comparison with plot M. On the other hand, cutting of higher intensity (H, CC) resulted in lower numbers of fructifying parent trees. The medium cut intervention having provided the plot M with stocking of 0.5 (50% of the stand) resulted in a lower number of seed resources (limiting factor for natural regeneration). However, for the other factor - seedling establishment (survival and recruitment) this plot (M) represents an ecological optimum in beech regeneration in the given conditions.

In the years 1999-2003 the effect of covering rows of early potatoes with non-woven fleece on the yield formation, dry matter content in tubers, temperature of soil and of air in ground layer was investigated. Covering of rows enhanced the market yield of tubers at early harvest 60 days after planting in average by 35.8% in comparison with the uncovered control. At harvest 67 days after planting the difference in advantage of the covered variant were 17.2% and 85 days after planting the difference in yield between variants was already non significant. The yield effect of the fleece was affected by year (higher effect in the years with cold spring) and by variety (Impala responded at 60 days after planting by yield increase under fleece 43.7%, Adora 27.9%). Higher dry matter content of tubers by 0.87% was found in covered plants 60 days after planting in comparison with control. Cover enhanced average air temperature in ground layer by 2.0°C, in soil in depth 100 mm by 1.8°C.

Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype P1.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed.

The present study was conducted to determine the effects of different pelleting temperature on tibia mineralization, calcium, phosphorus content of serum and growth performance of broilers fed phytase-supplemented diets. The basal experimental diet type was typical maize-soybean meal. The basal diet was supplemented with a microbial 6-phytase (Novo CT: coated thermostable granulate, from Novo Nordisk A/S,Denmark) at 500 Phytase Units (FTU) per kg of feed before pelleting. The diets were pelleted at 65, 75, or 85°C except the basal mash diet as the control. The experimental diets were used from 0 to 6 weeks of age of birds. Dry matter, crude ash, Ca, total P, Na, K and Mg, Zn, Fe, Mn, Cu contents of tibia were not affected by the pelleting temperature. No effects of pelleting temperatures on Ca content in the serum were found out. However, P content in the serum was increased by feeding the diet pelleted at 65°C as compared to the control and other treatments. Pelleting at 65 and 75°C had a significant positive effect on body weights and body weight gains of broilers. It was concluded that the best pelleting temperature was 65°C. It is to note that the temperature of 85°C damages enzymatic activity.

The effect of environmental conditions on hatching indices of poultry eggs is sufficiently important that new methods for their improvement are sought, among others through exposing the eggs during hatching to an artificially generated magnetic field of variable frequency. Hatching eggs in this study came from Pharaoh quail in the 4th month of laying. The flock was kept under optimum microclimate conditions and fed a complete feed mix containing 21% total protein and 11.7 MJ ME. The eggs (n = 150), after weighing, were divided into 3 groups equal in respect of numbers: control (I) and two experimental (II and III). The eggs of group II and III were exposed to the action of variable magnetic field of the same intensity but different times of application. The highest percentage of dead embryos during incubation in relation to fertilised eggs was found in control group (11.36%), while the smallest was from group II (4.17%). The highest value of hatching indices calculated in relation to fertilised eggs was found in group II (91.6%), while the smallest was in group III (83.7%) with 86.3% in control group. The results point to the possibility of increasing egg hatchability indices through the use of additional variable magnetic field. The chicks hatched from eggs exposed to the action of this experimental agent had similar body weight. The average weight of one-day-old chicks ranged from 7.82 g (group II) to 8.05 g (group III). In the last week of rearing, mean body weight in both sexes was similar and ranged from 168 (group I) to 172 g (group III) in males, and from 186 g (group I) to 199 g (group III) in females; these differences were not statistically significant. The females of group III reached sexual maturity at 41 days, this being one and three days (non-significantly) earlier than birds in group I and II.

In the course of a 3-year study the natural occurrence of storage diseases in ambient air storage with 1 to 2°C was evaluated on samples of 30 cultivars and advanced selections that were harvested from orchards with and without the use of fungicide treatments. Based upon the frequency of occurrence, bitter rot and grey mould were the most common diseases in this study followed by blue mould and brown rot. Fungicide treatments applied in the orchard with integrated plant protection reduced total fruit rotting with different cultivars two to four times. Significant losses caused by rotting usually took place in the last quarter of the normal storage life of each cultivar, but in the case of samples treated with fungicides it was mostly during the last month. Cultivars Angold, Gala, Florina, Melodie and Meteor proved to be partially resistant to the storage diseases, but the smallest shares of rotted fruits were recorded on apples of Zuzana andMelrose. Still, five advanced selections proved not to be so affected by fruit rotting asMelrose and Zuzana, from which HL 237 and HL 390 were the most remarkable. The majority of selections with resistance to storage diseases as well as Zuzana cv. contain Glockenapfel cv. in their pedigree.

"The International Year of Microcredit 2005 underscores the importance of microfinance as an integral part of our collective effort to meet the Millennium Development Goals. Sustainable access to microfinance helps to alleviate poverty by generating income…" (Kofi Anan). One of the important factors which influence disbursement of public debts in the LDCs are Microfinancing institutions. The article characterizes the progress of discharge of bankrupt from the macroeconomic point of view, and in the situation for microfinancing industry. One of the main question is when and under what conditions it is the suitable to accelerate the transformation of informal MFIs to formal institutions. In the conclusion, the basic conditions and hypothesis, which are necessary for functioning of formal MFIs, are mentioned.

In case that the climatic or farming conditions have changed in a region, it is possible to anticipate that the core parameters of drainage constructions will not be adequate for the current needs. Some of these constructions might be over-dimensioned, which would be inconsistent with current terms of nature and water resources protection. On the other hand, the valuable contribution the regulation of the water regime of originally waterlogged lands and swamps indisputably brought along and thus enabled the agricultural use of drained lands would be depreciated to a certain extent. In this paper, some scenarios of probable climatic changes in the Czech Republic's territory and the anticipated effect of these changes on the components of the hydrologic water discharge from drained agricultural and forest catchments are studied. The function of drainage systems on selected experimental lands is examined. However, not only probable changes in precipitation, temperature and water runoff should be taken into account, but also changes in the way of farming, i.e. changes in the agricultural conditions and data that played a decisive role in the calculation of the basic parameters of these construction projects, for instance, the spacing of parallel drains or trenches or the depth of their laying. In the Czech Republic about 1.1 million ha of total agricultural land was drained by the end of the twentieth century. In some localities of Eastern and Southern Bohemia up to 80% of agricultural land was drained. To what extent the above-mentioned climatic changes and the changes in the way of farming influence the drainage system and whether adjustments of these systems are required are the questions we tried to answer at least partly in this contribution. The effect of climatic changes on the hydrologic balance and/or on the runoff from the catchments significantly varies up to ± 150% provided that we compare average runoff. The influence of changes in the way of farming on the hydrologic balance manifests itself in a more concrete, but negative way, and that demands a concrete reaction on the part of the water management control. It is therefore vital to take such measures of regulation of the water regime of soil for the existing drainage systems that will ensure both the drainage phase and the phase of runoff retardation. Single-function and obsolete drainage systems should be converted into systems with controlled drainage and irrigation functions - the double-function control systems.

In the current study, intracellular (neutrophil and monocyte) concentrations of free and liposome encapsulated ampicillin in sheep were investigated. Free ampicillin (5 mg/kg b.w.) and liposome encapsulated ampicillin (5 mg/kg b.w.) were administered as a bolus intravenous injection to sheep. After the injections, blood samples (5 ml) were collected into tubes from v. jugularis at 10, 30, 60 minutes and 2, 4 and 8 hours. Neutrophils and monocytes were isolated, and lysed in distilled water. Ampicillin concentrations were measured by high performance liquid chromatography. The results indicate that liposome encapsulated ampicillin caused the higher intracellular concentrations within neutrophil (ratio of liposome encapsulated ampicillin/free ampicillin; from 1.393 to 5.416) and monocyte (ratio of liposome encapsulated ampicillin/free ampicillin; from 0.973 to 2.906) cells than free ampicillin, and liposome encapsulated ampicillin existed a longer length of time within neutrophil (4 hours) and monocyte (4 hours) cells than free ampicillin (60 minutes), as well. This formulation may be beneficial, in that the treatment of intracellular infections are caused by sensitive bacteria.

An experimental stand was established as a monoculture by planting Norway spruce on a clear-felled area in 1925. In 1958, a research thinning experiment was established there. Control plots and the study of their development without anthropic interventions are of interest in particular. The spruce monoculture nearly disintegrated and the stand-forming role was taken over by species which occurred there originally as admixed ones. The stand became sparser and it was associated with the spontaneous regeneration of broadleaves. Out of the eight control plots three plots were selected differing significantly in stocking and extent of natural regeneration. The development of regeneration was studied in detail on these plots. The undergrowth consisted of 24 woody species in a total number of 6,000 individuals per hectare and about two thirds of them belonged to trees. The results of the study show a possibility to convert a spruce monoculture to a broadleaved commercial forest using the spontaneous regeneration of trees.

The effects of soil conditioners Frisol, TerraCottem, Bi-algeen, and fertilisers Silvamix Forte and Cererit on the welfare of Austrian pine were observed in a seven-year experiment on Loket spoil bank. Simultaneously the economic aspects of growing the Austrian pine until the stage of established plantation were examined. The application of products one year and a half after planting did not cause seedling mortality. The elongation growth of young plants conditioned by the application of Silvamix Forte and Frisol was statistically significant whereas these effects manifested themselves in the second year: they persisted till the end of the experiment for Silvamix Forte, and for three years in total for Frisol. The other products - TerraCottem, Bi-algeen and Cererit - also showed generally better, though statistically insignificant results as compared to those of the control plants. The elongation growth for all products culminated in the fifth year and then declined. The stem diameter growth for the best products reached its maximum in the second year. Regarding the overall height growth after seven years, the average number of the highest plants on the experiment location was on plots treated with Silvamix Forte; these plants exceeded the control plants by 46.2 cm. Pines treated with Frisol were higher by 23.4 cm. However, the application of soil conditioners and fertilisers to the given location proved economically ineffective because there was no need to improve the soil at the location, the same applies to mowing and hoeing since almost no forest weed grew there and the substrate surface remained well-aerated and porous.

The priority of this study was to investigate how selenium influences toxicity of Cd, Zn, Cu and Pb when the metals appear in the hydroponic solution in pairs. As a model subject mustard seedlings (Sinapis alba L.) were used. Except phytotoxicity of individual metals and Se-metal combinations determined through photosynthetic pigments content (chlorophyll a, b, total carotenoids) also metal accumulation in the roots and shoots was determined by the AAS method. Se in all metal combinations reduced the unfavourable effect of other metals tested for chlorophylls content, however, for carotenoids primarily the opposite effect occurred. For metal accumulation in the roots and shoots it was confirmed that Se stimulated only Cd (about 24%) and slightly Cu (about 9%) accumulation in the roots, while in the shoots there was inhibited accumulation of all metals tested. The strongest inhibition was observed in Pb accumulation (84.9%). This fact indicates that Se generates some barriers for metal transfer from the roots to the underground plant parts. In contrast, Cu, Zn, Pb and Cd increased Se accumulation in the roots in the range of 4 (in combination with Cu) to 68% (in combination with Cd) and in the shoots in the range of 11 (in combination with Pb and Zn) to 44% (in combination with Cd). In the shoots only Cu inhibited Se accumulation (about 67%).

Fertilization with tannery materials positively influenced the soil pH and increased the content of alkaline cations in soil. After four years of investigations organic carbon content decreased most in the soil fertilized with untreated sludge, mineral salts and in the control soil. Irrespective of fertilization used and year of studies cadmium occurred mainly in most mobile fractions (mobile, exchangeable and bound to manganese oxides). Copper was mainly bound in fractions less available to plants. After four years of studies an increase in mobile copper forms was registered in all treatment soils. Lead in the soil was present mainly in the form bound to organic matter and amorphous iron oxides. Distribution of lead points to the small share of this metal in the mobile and exchangeable forms, both immediately after fertilization applied and after four years of studies. Chromium became fixed in fractions less available to plants, mainly bound to iron oxides and in residual fraction. On the other hand, an apparent effect of fertilization with materials of tannery origin on this element total forms content was registered.