Fulltext search in archive

The effect of some factors (genotype, sex, litter size, age of dam at lambing, month of lambing and year of birth of lamb) on the growth of lambs - crossbreds between the Improved Wallachian (IW) and East Friesian (EF) breed was evaluated in operating conditions during two successive years. Three genotypes were evaluated: IW 50 EF 50, EF 75 IW 25 and EF 87.5 IW 12.5. The evaluation of the effect of genotype on growth showed that this factor did not have a significant effect on the majority of growth traits under study. In the period from birth to 100 days of age the highest daily gain was found in IW 50 EF 50 (263 g). The daily gains of EF 75 IW 25 and EF 87.5 IW 12.5 in this period were lower but identical (244 g). Age of dam had a significant effect on the majority of growth traits under study. Sex of lamb had a significant effect on body weight at 100 days of age and also on the majority of daily gains under study. The factors litter size, month of lambing and year of lamb birth were the most significant sources of variation of daily gains. As far as the non-genetic factors were concerned, the highest daily gains from birth to 100 days of age were recorded in males (259 g), in singles (260 g), in lambs from 4-years-old ewes (269 g), in lambs born in January (263 g) and in lambs born in the first year of evaluation (256 g). The analysis of phenotypic correlations indicated that body weight at birth showed a positive and highly significant (P ≤ 0.01) effect on the majority of growth traits under study. All phenotypic correlations between individual body weights were positive and high (P ≤ 0.01). The majority of phenotypic correlations between individual daily gains under study were also positive and high (P ≤ 0.01).

At present the laying hen rearing is transformed into alternative housing systems which include a deep litter system. As for the poultry nutrition, the replacement of animal protein with vegetable protein in poultry feed is applied. Therefore, in an experiment we evaluated the quality of eggs from laying hens in the cage system as compared to the deep litter system with vegetable protein substituted for animal protein in their feed. 72 ISA Brown laying hens were placed into the experiment. 36 laying hens were kept in the deep litter system and 36 laying hens were kept in conventional cages for laying hens. For the period of 9 months, 36 eggs from the group in the deep litter system were examined, always at intervals of five weeks (i.e. 8 × 36 eggs) and so were 36 eggs from the group in the cage system (i.e. 8 × 36 eggs). We found out that in the deep litter system the mean egg weight was higher (P < 0.01), shell weight was lower (P < 0.01), egg albumen was higher (P < 0.01), yolk weight did not differ (P > 0.05), yolk cholesterol was higher (P < 0.01), yolk colour was darker (P < 0.05), shell strength did not differ (P > 0.05), all in comparison with the cage system of laying hen keeping. The results of the experiment show that where vegetable protein was substituted for animal protein in the nutrition of laying hens, there were differences in the quality of eggs from laying hens kept in the deep litter system and eggs from laying hens kept in the cage system.

Two experiments were conducted to investigate the effects of dietary crude protein level and degradability on milk urea nitrogen (MUN) content. In experiment 1, twelve multiparous lactating cows averaging 176 days in milk were divided according to DIM and milk production into three 4 × 4 Latin squares with four 2-week periods. Cows were fed four diets with different crude protein levels (13.0, 14.0, 15.0, and 16.0%, DM basis) with isocaloric, respectively. Crude protein levels had a low effect on milk yield and composition (P > 0.05), but a significant effect on MUN content. There were significant differences in the MUN content of cows fed either of the two diets (P < 0.01). In experiment 2, fifteen multiparous Holstein dairy cows averaging 91 days in milk were classified according to DIM and milk production into five 3 × 3 Latin squares with three 3-week periods. Cows were fed one of the three isocaloric and isonitrogenous diets with RUP being 30.8%, 36.2%, and 41.6% (CP basis), respectively. Milk yield, milk composition, and MUN content were not significantly affected by protein degradability, and there were no significant differences between any two dietary treatments (P > 0.05). These results indicated that MUN might be used as a parameter to monitor the change in dietary protein levels.

This paper will focus on the development trends of enterprise information infrastructure which will take into account an effective management of institution as well as integration of the existing technologies and systems. A special focus will aimed at the new Smart Enterprise Suites (SES), which should provide for convergence and integration of the originally single systems as portals, content management and collaboration. The author presented part of these results also at the Agrarian Perspectives Conference 2005 in the applied informatics session.

This paper deals with the analysis of the trends in costs and revenues of selected agricultural products grown and bred under the conditions of organic agriculture. The analysis of the trends in revenues and costs is performed for the cattle breeding, beef cows and for the plant production of spelt, oat and potatoes. Costs are evaluated in the relationship with the direct and indirect costs. Revenues are traced with the help of per hectare yield, efficiency and market prices. Data of the selected file of the organically farming companies for the controlled commodities are compared with the same commodities of the selected file of the conventionally farming companies worked by the Research Institute of Agricultural Economics (RIAE) in Prague.

The article presents original results of research on the humus form dynamics in a semi-natural European beech stand in the National Natural Reserve Voděradské bučiny, in the area of Training Forest Enterprise at Kostelec nad Černými lesy (Czech University of Agriculture inPrague). The accumulation and soil chemical characteristics of particular humus forms were studied: dry matter amount, pH, soil adsorption and exchangeable acidity characteristics as well as plant available and total nutrient contents. The humus form samples were taken in four replications from the particular humus form layers (L, F, H, Ah), and the analyses were performed individually. Samplings were done in the parts of the stand with closed canopy, and they were compared with the state in gaps formed during natural and semi-natural forest regeneration (the stage of stand breaking up left to spontaneous development). The results confirmed marked changes in the quantity and quality of surface humus in the gaps. The amount of dry matter decreased by ca. 25% several years after canopy opening, especially in the H horizon, the pH, base content and base saturation increased, as well as the content of macronutrients (with the exception of total calcium). The results proved considerable changes in the humus forms during the natural and semi-natural forest cycles connected with the stand regeneration.

Sera of 413 dogs and 286 cats from the CzechRepublicwere tested for antibodies to Toxoplasma gondii by the indirect fluorescent antibody test. The IgM antibodies to T. gondii were found in 10 (2.4%) dogs and 8 (2.8%) cats; IgG antibodies were found in 107 (25.9%) dogs and 126 (44.1%) cats. Of the dogs, the most exposed group were pet dogs, followed by police dogs; no antibodies were found in laboratory dogs. No statistically significant differences in prevalence were observed between clinically healthy (n = 115) and diseased pet dogs (n = 80); compare 0.87% and 1.25% for IgM, and 33.9% and 33.75% for IgG, respectively. Although T. gondii is a common parasite in domestic cats and dogs, the clinical importance is low.

In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from pigs, cattle, poultry, and slaughter houses. Three common techniques were used to isolate Y. enterocolitica from the samples - ITC, PSB, and direct on the CIN. Primers A1/A2, Y1/Y2, and rfbC 1/rfbC 2 were used for the specific detection of the pathogenic strains of Y. enterocolitica. Traditional microbiological methods were found to be insufficient for the specific identification of the Y. enterocolitica pathogen. In comparison with PCR which was able to detect 149 strains, the biochemical test could detect only 138 species. These results show that the use of biochemical methods of cultivation did not allow the identification of all Y. enterocolitica pathogens. In total, 149 strains of pathogenic Y. enterocolitica were examined of which 120 were from pigs, 19 from poultry, 8 were cattle strains, and 2 came from the environments of slaughterhouses.

Limited information is available regarding the source-sink alterations on soybean yield under whole plant light enriched conditions. The differential responses of yield components for two old and two new cultivars were investigated. The yield sensitivity of the old cultivars to the changes in source strength and light enriched conditions was much greater than that of the two new cultivars. The yield of the new cultivars was more likely sink-limited, and source-limited for the old cultivars. The increased yield by light enrichment was in part due to an increased branch contribution. Pod number per plant was more responsive than seed number per pod. The reduction in pod number by source-sink manipulation was more severe in the old cultivars. Light enrichment increased pod number in the two old cultivars but not in the two new cultivars. Seed size was responsive to changes in the source-sink ratio and changes in the environment. Both pod number or seed size may be increased or decreased if environmental conditions for seed filling are altered.

Field experiment demonstrated the benefit resulting from biological soil management including the use of N₂-fixing and growth promoting bacteria A. brasilense B-4485 for long-fibred flax. Seed inoculation by A. brasilense B-4485 was equivalent to the introduction of 15 kg/ha of N that provided the possibility of partial flax N requirement supply. Treatment A. brasilense + N₁₅P₆₀K₉₀ is considered to be the most profitable management in regard to flax yield and its quality, and is comparable to N₃₀P₆₀K₉₀ treatment. Biological management allows preventing high concentrations of N in soil, excludes lodging and negative effects on flax yield and its quality. Implementation of biological management for flax nutrition may be profitable for both ecology and economy of long-fibered flax growing.

The objective of this paper was to obtain information about the effect of interactions and different agronomical measures on autumn saved herbage of different sites in Central Europe (Czech Republic, Germany, Hungary, Poland). Within the different sites, the effect of the factors pre-utilisation (accumulation since June, July or August) and winter harvest date (November, December or January) on dry matter (DM) yield and digestibility of organic matter (DOM) was investigated over three consecutive years. All results were examined by analysis of variance and least-significance differences (LSD) were calculated (with P < 0.05). For all years, date of winter harvest was the most important source of variance. The highest yields were attained in November (varied from 2.3 to 0.8 t/ha), the lowest in January (from 1.5 to < 0.2 t/ha). The highest DOM concentration (from 70.4 to 52.4% of dry matter) was reached in November, and it decreased until January (from 59.6 to 35.2% of dry matter). Predominantly caused by the prevailing climatic conditions, the DM yields of the sites varied considerably during winter, showing a significance for the interaction winter harvest date × site as well as for the factor site. The utilisation of autumn saved herbage, pre-utilised in July, might be the best opportunity providing an adequate quantity and DOM for suckler cows until the end of the year, but the prevailing climatic conditions at different sites inCentral Europe finally decide on the practicability of winter grazing.

Physical properties of commercial fertilizers play important role from precision application point of view. Granulometric evaluation is usually performed by sieve separation according ČSN 01 5030 standard. The main subject of this work is the presentation of separation results when vertical airflow is used.

In ten cultivars of apple fruit, ethylene production expressed in μl/kg/h was determined. The cultivar Resista exhibited a higher ethylene production and can be differentiated from other cultivars. The production ranged from 4.2 ± 0.58 μl/kg/h in the case of Meteor cv. up to 131.6 ± 5.5 μl/kg/h in Resista cv. Infected fruit of Topaz cv. had a lower ethylene production at cold storage temperature (3°C) than some healthy fruit. All examined cultivars can be divided into three clusters. Discriminant analysis and canonical correlation analysis of the examined apple fruit led to the determination of healthy and infected fruit. Values of ethylene production were analyzed on intact fruit by using headspace gas analysis by CGC with thermal desorption technique. Carbosieve G was chosen as the adsorbent material for the traps due to its relatively high affinity for light hydrocarbons such as ethylene. For a full trap of ethylene in the enrichment column the sufficient amount of percolating gas is about 0.3 l.

In this study, the compatibility of the pear cultivars PI1/6 and BC Williams with 49 quince genotypes was evaluated. The results showed that the cv. PI1/6 had a good compatibility with several of the quince genotypes, and the cv. BC Williams only with a few of them. Differences were also found in their susceptibility to fire blight (Erwinia amylovora). The cultivar PI1/6 was very susceptible, while the cultivar BC Williams was relatively tolerant.

The consumption of the organic food in the Czech Republic is, in spite of its permanent growth, still on a very low level. The reasons are both on the side of supply and demand of organic food. The reasons on the side of the supply are especially the insufficient amount and the structure of the organic products. Demand is not met especially in the case of the livestock products, further also in the fruits, vegetables, bread, pastry and others. The lack of the organic food is solved by imports. The important factor is also the lack of the processing capacities, especially for meat and dairy products. The sale is provided under the way of short-term contracts. The prices that the organic farmers get for their products fall short of the quality of products. The main distribution channels are the supermarkets. The organic products are here, however, only the marginal products. For the increase of the supply, the advertising and advertisement should be intensified. The demand is obstructed especially by the low consumer awareness, while the consumers are not sufficiently familiar with the quality of the organic products and are not willing to pay higher price for the higher quality of foodstuff.

A 10-week-old, 4.5 kg female Weimaraner dog was referred to theChonbukNationalUniversity, Animal Medical Centre with signs of regurgitation after weaning. The cervical oesophagus was palpable as a flaccid, air-filled cavity. The thoracic radiographs revealed oesophageal dilatation cranial to the heart and constriction at the level of third rib. A presumptive diagnosis was made as persistent right aortic arch (PRAA). A left 4th intercostal thoracotomy was performed and the definitive diagnosis was made as PRAA with left ligamentum arteriosum (LA) and an aberrant left subclavian artery (SA). The oesophagus was found dually compressed and severely necrosed. The corrective surgery comprised of transection of the LA as well as resection and anastomosis of the oesophagus, which resulted in a complete alleviation of the clinical signs.

The population dynamics of the horse-chestnut leafminer Cameraria ohridella Deschka et Dimić. was investigated in 1997-2000. The mortality of wintering pupae and the rate of fungal infections were also examined. The mortality of the horse-chestnut leafminer was nearly 40% at the end of spring. The spectrum of fungi on examined dead pupae was as follows: Verticillium lecanii (Zimmerman) Viégas, Cladosporium herbarum (Persoon: Fries) Link, Cladosporium cladosporioides (Fresen.) de Vries, Alternaria alternata (Fr.) Keissler, Acremonium strictum W. Gams, Rhizopus stolonifer (Ehrenberg: Fries) Lind., Arthrinium phaeospermum (Corda) Ellis and Acremonium sp. 1. Verticillium lecanii was the only identified fungus with an entomophagous status. The fungus is, however, a well documented entomopathogen of Homoptera, particularly of aphids, scale insects and whiteflies in tropical and subtropical regions.

The aim of this study is to reveal the morphology of the lyssa in the cat and dog. Eight heads of adult healthy cats and eight heads of dogs of both sexes were used as materials. In the cat the lyssa, yellow coloured, had a helical appearance and its edge facing the oral cavity became sharp; in the dog the lyssa, pinkish white coloured, was more or less J-shaped. The whole body of the cat's lyssa was buried among the intrinsic lingual muscles. In the dog, although aboral, two thirds of the lyssa were squeezed among the intrinsic lingual muscles, its cranial third was placed just under the mucosa to protrude slightly into the oral cavity. In both species, the whole body of the lyssa was determined to have been formed by the nearly adipose tissue in which occasional striated muscles existed. Moreover, in the middle third of the dog's lyssa, dense striated muscle fibres were seen dorsally to the adipose tissue, and we also noticed with interest that the lyssa sheath embracing this part contained few muscle spindle-like structures. It was of interest that in the cat a pyramidal rod encircled by a fine capsule of connective tissue was attached to the ventral edge of the cranial third of the lyssa.

The article investigates the socio-economic importance of collection and use of non-wood forest products (NWFP) for inhabitants in the Czech Republic (CR) in 2005 and brings a comparison with former investigations and research projects. The research project consisted of these parts in 2005: 1. Analysis of hitherto performed research projects and their results in 1999-2003, 2. Investigation of quantities and values of NWFP collected in 2004 and 2005, 3. Specific investigation of socio-economic importance of NWFP collection and use. The results of respective investigations prove NWFP collection being quite important for the inhabitants of the CR. The importance consists in the material value of picked commodities on the average annual level higher than 3,000 mil. CZK. Nevertheless, collecting NWFP is considered as a very important recreational activity. Only a small part of the population collecting NWFP (about 12%) collects NWFP to save money.

At the end of nineties, within the mountain forests of the Krušné hory Mts., in connection with an increased acid deposition, the symptoms (yellowing) started to be visible, characterizing magnesium deficiency in the assimilation organs, when Mg contents were laying under 300 mg/kg. In mineral soil the Mg content is mostly less than 10 mg/kg. Contents of other basic elements (Ca, K) were also very low. During four years yellowing symptoms of the spruce stands have developed in an extent area of more that 10,000 ha. Since 1999, preventive measures have been adopted to improve the nutrition status of the forest stands. Liquid magnesium fertilizers as MgNsol, Lamag Mg, MgSsol were applied during the period of 1999-2002. Since 2001, in semi-operational experiment, powder Mg fertilizer - Silvamix F4 - of higher Mg content (63.3% MgO) was applied. Application of liquid fertilizer MgNsol has helped to compensate the nitrogen deficiency in the stands of insufficient nutrition in this element. Comparing of different types of liquid fertilizers has confirmed, that the highest increase of magnesium in assimilation organs was find in combined application of MgNsol and MgSsol fertilizers. Application of 300 kg/ha Silvamix F4 has increased the magnesium content in the first needle year class in 73% in average, potassium in 33%, calcium in 16%, and phosphorus in 85%. Besides increased nutrient content in needles also improvement of the health and phosphorus in 85%. Besides increased nutrient content in needles also improvement of the health state, i.e. revitalization of the stands affected can be observed.

A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied utilizes the sequence of the mitochondrial gene coding cytochrome b which is specific for mammals. It uses the common forward primer and the reverse primer species-specific. After electrophoresis, cow DNA was characterised by the fragment of the size of 274 bp, goat DNA by the fragment of 157 bp, and sheep DNA by the fragment of 331 bp. The detection limit of the PCR method described (1%) was determined with model samples made from pure goat cheese with a defined addition of cheese made from cow's milk. The method validated was applied in the analysis of 17 goat cheeses and 7 sheep cheeses obtained from retail trade. Products of Czech, Slovak, French, Dutch, and Italian origin were examined. The presence of undeclared cow's milk was detected in three kinds of goat cheese and in one of sheep cheese.

The effect of nitrogen fertilization on root length (RL) distribution of winter wheat (Triticum aestivum L.) was investigated. The study was conducted in Prague-Ruzyne on clay loam Chernozemic soil in the years 1996-2003. Two (N0, N1) and three (N0, N1, N2) treatments, unfertilized (N0), fertilized with 100 kg (N1) and 200 kg N/ha (N2) were studied in 1996-2000 and 2001-2003, respectively. Nitrogen rate 100 kg/ha had no effect on RL in soil layers (P > 0.1) in years 1996-2000 and 2002-2003 and there was not significant interaction between N treatment and soil layer except for year 1998 (P < 0.01). Nitrogen fertilization affected RL distribution significantly (P = 0.013) only in 2001 due to reduction of root growth in subsoil layers in treatment N2 (200 kg N/ha) in comparison with N0 and N1. The effect of N fertilization on total RL in rooted soil volume was insignificant. There was a significant effect of year on total RL (P < 0.01) but not of interaction of year and N treatment. Roots reached, with the exception of two years, the depth between 100 and 130 cm. Nitrogen fertilization (N1) had no effect (P = 0.59) on rooting depth (RD) in years 1996-2000 but there was a significant effect of interaction between year and N fertilization on RD (P < 0.01). In the second experimental series (2001-2003) N fertilization rate 200 kg N/ha significantly reduced maximum RD (P < 0.01) in comparison with N0 and N1. The year had highly significant effect on RD.

Based on NIRS (near infrared reflectance spectroscopy) measurements carried out in a collection of 262 samples of winter oilseed rape with a different content of fatty acids (FA) in oil, calibration equations for the laboratory instrument Foss-NIRSystem 6500 were developed. Calibration was focused on the possibility of screening seed samples of different composition of oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3) using NIRS analysis. The reference method was gas chromatography (GC). The content of FA in segregating F₂ generations after crossing between lines with different contents of FA and lines with standard content of FA in oil ranged from 32.3 to 82.0% for C18:1, 10.2-26.8% for C18:2 and 3.3-11.8% for C18:3. The verification of a validation equation in 50 randomly selected samples of F₂ generation in the year 2006 proved high correlation coefficients (r) between NIRS analysis and GC values, r = 0.86 for C18:1, r = 0.82 for C18:2 and r = 0.85 for C18:3. Non-destructive NIRS analysis enables rapid and reliable selection of materials with different composition of FA in the seed of oilseed rape (lines with desirable high content of C18:1 and low content of C18:3).

Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size, was used. Apparently healthy palms in two coconut gardens were tested for Ganoderma disease by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the disease. Based on the ELISA results, Pseudomonas fluorescens + Trichoderma viride with chitin amended treatments arrested the multiplication of the pathogen and within 6 months showed an optical density (OD) below the level of infected plants. Integrated Disease Management (IDM) and fungicide tridemorph treated palms showed OD values below infection level within 7 months, and T. harzianum and P. fluorescens + T. viride treated palms showed OD values below infection level in 8 months.

Twenty-four Czech dairy farms were visited to record lameness prevalence and to identify factors associated with high lameness prevalence at the farm level and/or increased lameness risk at the level of individual cows. All cows were checked for lameness and forty cows per farm were examined for overgrown claws, body dirtiness and skin lesions. The farm environment was scored between 1 (excellent) to 5 (very poor) in three different aspects: floor slipperiness, cow care quality, and housing quality. Data on hoof trimming schedules were obtained from farm managers. Lameness prevalence on farms was in a wide range from 6% to 42% (median 22%). At the farm level, floor slipperiness and poor animal care were associated with high lameness prevalence (Spearman correlations, P < 0.05), and the proportion of cows with overgrown claws tended to be associated with it (P < 0.10). The reported time schedules of hoof trimming (continuous trimming applied or not; and time elapsed since the whole herd was trimmed) were unrelated to either the prevalence of overgrown claws or the prevalence of lameness. Within farms, cows with overgrown claws and dirty cows were at an increased risk of being lame (multiple logistic regression, P < 0.05) and cows with skin lesions tended to be more lame (P < 0.10). The risk of lameness had an inverted U-shape dependence on age (P < 0.05), with cows at 7-8 years of age being the most endangered by lameness. We conclude that there is a large potential for lameness reduction on some Czech dairy farms through improving the cow care and reducing floor slipperiness, and that within farms, cows with overgrown claws and also dirty cows and cows with skin lesions should be given special attention since they are more likely to get lame.

In this study the change in amino acid profile in cow's colostrum and transient milk during the first week after parturition was examined in a Hungarian Charolais herd. Experiments were carried out with n = 37 Charolais cows in the same herd in the spring (March-April) of two consecutive years (Experiment 1: 2002, n = 15; and Experiment 2: 2003, n = 22). Colostrum and milk samples were taken by hand milking immediately after delivery, and in 24, 48, 72, and 168 hours post partum. Amino acid contents (%) in samples were measured in milk protein with an automatic amino acid analyser. Data were processed by the software of SPSS.10 statistical program package. In the postpartal period, among essential amino acids significant increases were recorded in methionine, isoleucine, lysine, and phenylalanine, and among non-essential amino acids glutamic acid and proline increased significantly. Simultaneous decreases were recorded in valine, cysteine, aspartic acid, serine, glycine, and arginine. Inconsistent figures were determined for histidine, leucine, tyrosine, and alanine content between Experiment 1 and Experiment 2.

Putting together work operations minimizes the number of machine passes across the plot, which helps to reduce negative soil compaction and to save fuels. However, the combination of working operations also reflects in the increased weight of machines, which - on the other hand - can result exactly in soil compaction. This is why the potential adverse phenomenon can be compensated by using tyres with a larger contact surface with the base. In the case of sowing root crops, some problems may appear with the application of these tyres as a certain part of the stand has been sown in their track. The paper brings an assessment of the possibility to use twin assembly tyres on the tractor model Fendt 822 and on the sowing drill model Monosem NG plus with 18 drilling mechanisms. Parameters to be assessed were soil compaction, and the development of plants sown inside and outside the tractor track. Although the degree of soil compaction was higher in the tractor track, the biological characteristic of plants including yield reached more favourable criteria of assessment. The situation paradoxically resulted from the creation of more favourable moisture conditions in the soil.