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For the first time in the Czech Republic, bacteria identified as Pseudomonas marginalis, Pectobacterium carotovorum subsp. carotovorum and Pseudomonas putida were isolated from tubers of Zantedeschia spp. with symptoms of tuber soft rot. The symptoms occurred on mother tubers as well as on new daughter tubers of different calla lily hybrids with yellow spathe, calla lily cv. Mango with bright orange spathe and Zantedeschia rehmanii with pink spathe. The percentage of diseased plants of the total plants in the plot was around 10%. When inoculated into potato tuber slices, strains of P. marginalis and P. c. subsp. carotovorum produced soft rot. Pectolytic activity of P. marginalis strains was less intensive than that of the P. c. subsp. carotovorum strain. The results confirm that bacterial soft rot of Zantedeschia spp. may have several causes.

The role of the water movement and retention during extreme runoff formation was demonstrated in the Modrý Důl catchment (Krkonoše Mts., Czech Republic). A cyclone, which moved from Hungary to Poland, caused an extreme rainfall (120 mm) and subsequent extreme runoff in August, 2002. The precipitation, discharge, air and soil temperatures, tensiometric pressure, and soil moisture were recorded. The maximum retention capacity of the catchment was evaluated (70 mm). Depending on the actual retention capacity and the precipitation amount, two situations were recorded: (1) the precipitation amount lower than the actual retention capacity where the precipitation was fully absorbed in the catchment and the discharge in to the stream was not influenced by rain, (2) the precipitation amount higher than the actual retention capacity where the precipitation caused a saturation excess of the soil profile, generating extreme outflow into the stream. Neither the soil cover in the catchment or fluvial deposits along the Modrý potok stream were able to retain the extreme rain and inhibit the catastrophic flood.

Colic was treated surgically in 576 horses (545 individuals). Twenty-seven horses were subjected to surgery twice and two horses three times during the period of this study. A total of 371 horses (64.4%) were discharged from the hospital, 205 animals (35.6%) died or were euthanised; 16 of them died during anaesthesia, 102 horses were subjected to euthanasia during surgery, 24 patients did not recover from anaesthesia after surgery completion, and 63 horses did not survive the postoperative period. Ileus of the small intestine was diagnosed in 267 cases (46.4%), affection of the large colon in 239 cases (41.5%), lesion of the small colon in 22 cases (3.8%), lesion of the caecum in 19 cases (3.3%), and affection of stomach and rectum in four and one cases, respectively. In 14 animals (2.4%), lesions were located at two different sites of the gastrointestinal tract. In four horses, the cause of colic was located outside the gastrointestinal tract. Three animals were affected by diffuse peritonitis. No gastrointestinal lesion could be identified during surgery in three horses with recurrent colic. The most common causes of small intestine ileus included incarceration in inguinal hernia (50 of 267 horses, 18.7%), hernia of the omental foramen (31 of 267 horses, 11.6%), anterior enteritis (19 of 267 horses, 7.1%), mesenterial volvulus (18 of 267 horses, 6.7%), and ileal impaction (18 of 267 horses, 6.7%). The most common caecal disorder was acute constipation/dysfunction (5 of 19 horses, 26.3%). Frequent causes of the large colon colic were torsion (63 of 239 horses, 26.4%), left dorsal displacement (36 of 239 horses, 15.1%), and right dorsal displacement (23 of 239 horses, 9.6%). The small colon was most often affected by focal obstruction/constipation (9 out of 22 horses, 40.9%). Surgical treatment of colic of the small intestine, caecum, large colon, and small colon was successful in 59.6%, 36.8%, 73.3%, and 63.6% of the cases, respectively.

The agricultural production in less favoured areas (LFA) should, first of all, fulfil the non-production goals, as is the nature and environment protection and job opportunities generation. These goals could be achieved within the conditions of sustainable agricultural production systems. This paper is aimed on the analysis of how the companies operating in marginal conditions realize commercial principles and targets specified for the LFA conditions. The obtained knowledge indicates that the managers of majority of the companies do not respect the recommendations and persist in the intensive production in spite of the unfavourable conditions.

The present paper is the first contribution from the biomass quantification series which is realized by Forestry and Game Management Research Institute in the Krušné hory Mts. (Northern Bohemia). This study is aimed at blue spruce substitute stands. Research was done within the blue spruce experiment Fláje II in the Krušné hory Mts. (800 m above sea level in the spruce forest vegetation zone, acidic category). Results showed that the aboveground biomass of the investigated substitute blue spruce stand without thinning amounted to approximately 56 thousand kg of dry matter per ha at the age of 22 years. Wood and bark of branches are the most important parts of the aboveground biomass (ca 40%). Needles and stem wood accounted for approximately 26 and 28% and stem bark only for 6%. At the age of 22 years, the investigated substitute blue spruce stand accumulated: N - 336 kg, P - 28 kg, K - 138 kg, Ca - 159 kg, Mg - 28 kg per hectare. Thinning with the consequent removal of aboveground biomass (54% of trees, 40% of basal area at the age of 16 years) represented a loss of ca 8.7 thousand kg/ha of total biomass, which contained 53 kg of N, 5 kg of P, 22 kg of K, 26 kg of Ca and 4 kg of Mg. The removal of biomass in areas previously degraded by acid deposition may result in the deficiency of Ca and Mg because of their low content in forest soil. On the other hand, thinning supported the faster growth of trees left after thinning and consequently faster biomass and nutrient accumulation.

Our objective was to determine which rhizome extract from Japanese knotweed, Giant knotweed or Bohemian knotweed has the most significant inhibition effect on the germinated seeds. The seeds of white mustard were incubated with the extracts for two days under laboratory conditions. We monitored differences in number of germinated seeds, length of radicles, hypocotyls and root/shoot ratio between the control and experimental seeds. Inhibitory effect of extracts from dried knotweed rhizomes was confirmed, but without differences among tested plants. A higher allelopathic effect was revealed in the case of extract from aboveground parts.

Otiorhynchus sulcatus is considered as an important pest of strawberry fields and on potted ornamental plants. The efficacy to control this pest by new Polish products containing azadirachtin and the entomopathogenic fungus Beauveria brongniartii were tested. The aim of the laboratory investigation was to assess the influence of different forms of azadirachtin (A and B) and the entomopathogen on mortality and physiological development of the insect. Mortality after treatment ranged between 86-93%. There were significant differences in the mean number of surviving stages of the insect between Beauveria and neem treatments, but the final total mortality was not statistically different. Sensitivity of all developmental stages to the fungus was observed. In the neem treatments the physiological development of larvae was completely stopped. The level of control achieved by Beauveria and neem for use in outdoor containers is acceptable for practical application.

The objective of this paper is to evaluate the contents of CF, NDF, ADF and ergosterol in the forage of Fistulolium, Dactylis glomerata and Arrhenatherum elatius from stands harvested at the end of the growing season. The contents of CF, NDF and ADF were analysed using the ANKOM Fibre Analyzer instrument. The ergosterol content was analysed by the HPLC method. The lowest CF content was in the Festulolium forage matter (26.8%). Conversely, the highest content was in the Arrhenatherum elatius forage (30.2%). The CF content gradually increased during autumn from 28.0% to 29.4%. Likewise, the NDF and ADF contents were lowest in Festulolium (58.3% and 32.3%, respectively). The highest NDF content was in Arrhenatherum elatius (60.5%). The ADF contents in Arrhenatherum elatius and Dactylis glomerata were comparable (35.9% and 35.1%, respectively). The NDF content increased during autumn from 56.4% to 62.0% and ADF content from 32.8% to 36.4%. The ergosterol content suggests a lower infestation of the Festulolium forage by fungi. While this content in the Festulolium forage was 94.9 mg/kg of dry matter (DM), in the Dactylis glomerata forage it was 136.7 mg/kg DM and in the Arrhenatherum elatius forage 139.9 mg/kg DM. Forage samples taken in December contained ergosterol on a level of 248.6 mg/kg DM. The species under study and the time of use in autumn exhibited a statistically highly significant effect (P < 0.01) on the contents of CF, NDF, ADF and ergosterol in the forage matter.

Pollen viability and germinability in some European chestnut genotypes was assessed in this study. In 10 chestnut genotypes, percentages of pollen viability were generally high and often around or over 80%. The pollen germination percentages of the genotypes were significantly affected by media sucrose concentrations. At optimum sucrose concentrations pollen germination percentages varied between 21.97 and 43.68% in 2004, 3.95 and 31.97% in 2005 and 6.79 and 31.03% in 2006, across all genotypes. The highest pollen germination percentage was obtained from 10% sucrose concentration in all years. Although, in 2006, a highly marked positive correlation (r = 0.80) was determined for the viability and germination percentages, no significant relation between the viability and germination percentages r = -0.54 and r = -0.05, respectively) was found in 2004 and 2005. In 2005 and 2006, germination percentages declined compared to 2004.

A two-years-old, intact female, Shih-Tzu dog weighing 5 kg was presented to the Chonbuk Animal Medical Centre, College of Veterinary Medicine, Chonbuk National University, with the history of bone ingestion before two days. The survey radiographs of the thorax revealed a bone at the caudal thoracic oesophagus. An oesophagram was performed which did not reveal any oesophageal leakage or perforation. The left caudolateral thoracotomy was performed and the caudal oesophagus was found partially necrosed, friable and inflamed. The necrosed, friable part was removed and the oesophageal defect was reconstructed using a full-thickness muscle flap collected from the diaphragm. A part of the omentum was mobilized from the abdomen and sutured over the reconstructed site of the oesophagus to aid healing. Oesophagoscopy after nine days postoperatively showed a good adhesion of the diaphragm flap. The patient showed normal activity after 12 days and no complications were observed during a one-year follow up period. The pedicle flap collected from the left hemidiaphragm in addition to the omentum flap can be successfully used to reconstruct the circumferential oesophageal defect.

Objective of the study was to assess histopathological changes in horses with a clinical history of exertional rhabdomyolysis (ER) with special reference to polysaccharide storage myopathy and to compare histopathological findings in horses with and without a clinical history of ER. In total 39 muscle samples were collected, from horses with a history of repeated episodes of exertional rhabdomyolysis (test group, 10 horses) and from horses without clinical signs of muscular disorders in their history (control group, 29 horses). Frozen muscle samples were stained with haematoxylin and eosin and periodic acid-Schiff with and without amylase digestion. Histopathologic changes (amylase resistant polysaccharide, subsarcolemmal glycogen, intracytoplasmic masses, subsarcolemmal vacuoles, fibre size variation and internal nuclei) were evaluated. There was a statistically significant difference between groups in the presence of subsarcolemmal amylase sensitive glycogen deposits (P ≤ 0.0001), the risk ratio was 5.22. Statistically significant differences between groups were not found regarding the presence of intracytoplasmic masses, subsarcolemmal vacuoles, amylase resistant polysaccharide, fibre size variation and internal nuclei. Presence of amylase resistant polysaccharide within muscle fibres of apparently healthy horses could be a manifestation of different phenotype expression of PSSM but also the insufficient specificity of this diagnostic criterion.

www technologies are developing very fast. The new technologies make possible the easier creation of the web applications, which support business activities. Small and medium size enterprises, which serve as the driving force for innovations and thus for economic development, can utilize these technologies for the creation of relatively inexpensive applications, which can be tailor-made for the given enterprise by using the open source software (OSS). This contribution presents several practical examples of how to create - update - the web site of an enterprise and how to supplement it with an e-shop option. Examples from the area of flower selling have been selected.

The objective of this paper is the analysis of the macroeconomic aspects of regional development in the Slovak Republic. Regional development is a much frequented topic recently. In the pre-accession period, Slovak regions had the opportunity of benefiting from the pre-accession funds and competing for finances through projects and strategies. The EU support did not stop with the accession, it continues with the increasing intensity and variability at present. The real challenge is how efficiently is this support used and if it goes to the destinations where it is most needed. For the purposes of the identification of regional development tendencies, we have tried to analyze the selected macroeconomic characteristics for Slovak regions at the NUTS III level. We analyze the following indicators: GDP per capita, labor productivity, foreign direct investment, so that we can explore the situation of living standards in the regions and the determinants. We use the cluster analysis approach in order to specify and identify the regions with similar development characteristics. Based on our findings, we make some recommendations on the support and development strategies for Slovak regions.

Harvester technologies represent the second most common logging system in the Czech Republic. The high productivity of this technology is very necessary to cover its acquisition and operational costs. A human factor - a machine operator is the most important factor that surely influences the machine productivity. That is why the aim of this study is as follows: to analyze the mutual dependence of machine operator's education and working experience and harvester and forwarder productivity. The analysis proves that the operator's education and even more his working experience are very important. The time of harvesting work stages was measured when the harvester was operated by an operator with two-year working experience and by an operator with no experience. The average time difference between the harvester operators was 64.9 seconds in one work stage. The statistical significance of different operation times was confirmed during technically demanding segments in the working stage (the boom out the felling head and tree processing). Operators with the same working experience in forestry mechanization - about 13 years but with different education carried out the haulage work stage. In this case, the statistical significance between calculated differences was not proved. This fact proves that the operator's working experience is more important than his education.

The main chemoprotective polyphenolic compounds in the Vitis vinifera berries, rachis, and pedicels of 10 cultivars classified for the production of wine and growing in Southern Moravian vineyards, the Czech Republic, were studied. The following compounds were determined in the frozen fresh berries: gallic acid (1.8-13.3 mg/kg), catechin (70.3-659.1 mg/kg), epicatechin (67.1-237.2 mg/kg), trans-resveratrol (0.1-1.5 mg/kg), and pterostilbene (in traces); in the freeze-dried rachis and pedicels: rutin (10.5-68.6 mg/kg), isoquercitrine (29.8-218.3 mg/kg), catechin (283.7-2227 mg/kg), epicatechin (47.2-215.2 mg/kg), trans-resveratrol (2.6-37.1 mg/kg), and pterostilbene (0.01-0.13 mg/kg), respectively. The contents of polyphenolic compounds were different in various cultivars. The highest levels of catechin and epicatechin were found in the grapes of cv. Blauer Burgunder (3195 mg/kg), in which the second highest content of trans-resveratrol (33.2 mg/kg) was also found. The content of pterostilbene in the whole berries or stems was estimated for the first time. The rachis and pedicels could serve as a prospective source of polyphenolic compounds.

This report assesses the structural diversity of the spruce virgin forest in Nefcerka NNR in the Tatra National Park (TANAP). The structure of the virgin forest is evaluated by the indices proposed by Clark & Evans, Füldner and Jaehne & Dohrenbusch. Concerning the spatial distribution of trees (Clark & Evans index), a statistically significant difference was confirmed between the growth stage and the optimum and the breakdown stages. The influence of altitude on the tendency of concentration of virgin forest trees was also confirmed. In the case of complex diversity evaluation by the JAEHNE & DOHRENBUSCH index (1997), a statistically significantly different diversity of the spruce virgin forest between the growth stage and the other stages was found. The analysis of the regeneration processes revealed their good dynamics even at an altitude above 1,400 m, and with the ascending altitude (above 1,300 m) the dead wood and knolls of wind-thrown roots have the greater importance as seedbeds.

Grape anthocyanins not only play an important role in the colour quality of red wines but they also have many beneficial effects on human health, e.g., reduction of coronary heart disease incidence, or anticarcinogenic and antioxidant properties. Therefore, a rapid and efficient extraction technique prior to chromatographic analysis is of primary interest. Pressurised Fluid Extraction (PFE) presents a fast, effective, and environmentally friendly extraction method for the analysis of red grape pigments. In this study, PFE in static mode was utilised for the extraction of 3-monoglucoside anthocyanins from the grape skin of highly pigmented variety Alibernet. The effects of the type of the extraction solvent and the extraction temperature were studied. The identification of the above given compounds were performed by high-performance liquid chromatography with diode array detection (HPLC-DAD) based on Synergi C-12 column separation. The wavelength was set at 520 nm. All compounds were determined and identified during 50 minutes.

By reference to the Eternal Rye trial in Halle, Germany, as an example, it is demonstrated that long-term trials provide indispensable information for contemporary and future land use research. These trials serve as tools for the examination of cultivation measures or the effects of climate on nutrient dynamics and mobilization, microbial biodiversity, mineral composition or soil formation processes. They are therefore essential for the evaluation of land-use strategies or climatic change and, because of that, can provide more accuracy in related political considerations.

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8-11.2 × 10⁴ protoplasts/ml in darkness at 25°C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7^th and 11^th day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5-1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA₃, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69-75% in B. oleracea and 2-3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments.

: For the measurement of the infiltration speed under operational conditions, we were equipped by a rain simulator with the measuring surface of 0.5 m². The infiltration speed is determined from the defined rain intensity and water surface runoff from the measured surface. The retained water mass from the surface runoff is recorded at regular time intervals over the whole measuring period. The beginning of the water runoff from the measured surface indicates the beginning of elutriation. The measuring time is finished after the infiltration speed has been stabilised. The beginning of elutriation and infiltration speed stabilisation are typical and mutually comparable parameters for defined soil properties at the site followed.

This article is divided into four parts: the first is concerned with the state subsidy and support policy in the year 2001. The second part of the contribution mentions the subsidy development in production and marginal areas in the period 1996-2001 and its influence on the economic result of an average farm. Another part monitors the grant volume and the economic result at a selective sample of the identical firms in the time period from of the year 1997 to the year 2001 in reference to the farm position. The last part will deal with an evaluation of the grant volume where the subsidies are divided according to the subsidy titles in the year 2001 in an average agricultural firm farming in a certain altitude.

Interstitial pneumonia (2/3 of the lungs were affected) and diffusely enlarged bronchial and mediastinal lymph nodes were diagnosed by gross examination of a dead 16-year-old mare. Based on histopathological examination and the detection of acid-fast rods after staining by the Ziehl-Neelsen technique, tuberculosis was suspected. Mycobacterium avium subsp. avium of serotype 2 and IS901+/IS1245+ genotype was isolated from the pulmonary lymphnode after five-week incubation at 37°C. Due to the fact that horses have a naturally high resistance to mycobacterial infections, the high age of the mare most likely contributed to the development of the disease.

A mixed infection with Mycobacterium avium subsp. paratuberculosis (MAP) and Mycobacterium avium subsp. hominissuis (MAH) in one naturally infected red deer stag from a game park is described. The animal was euthanized because of symptoms of poor condition, weight loss and chronic diarrhoea. In spite of that, pathological lesions were observed only in the mesenteric lymph nodes, which were five to ten times enlarged with confluent caseous granulomas of 1 to 10 mm in size. Mycobacteria were isolated from all studied samples: a mixed infection of MAP and MAH was confirmed by multiplex PCR for the detection of IS 900, IS9011, IS1245 and dnaJ. MAP of the identical IS900 BstEII RFLP type C1 was isolated from all tissue samples and faeces. MAH isolates were detected in six examined tissue samples, including three mesenteric lymph nodes with caseous granulomas. Only minor differences in the band numbers and position of four different IS1245 PvuII RFLP patterns of MAH isolates were found. It follows from these results that red deer may potentially be infected with MAH, when a MAP infection is under way.

Zemspol, Dešná, Ltd is a large corporation engaged in various aspects of agriculture in the Czech Republic, including agricultural production, the assembly, maintenance and repair of agricultural machinery, the production of feed stuffs and feed mixtures,special agricultural services demanding special entitlement, business related matters and accounting. This paper focuses on agricultural production. The farm manages 2 100 hectares of agricultural land suitable for growing potatoes, although the largest area is used for growing wheat. The range of produce remains constant. Regarding livestock, the corporation specializes in cattle with the steady head count 250 for the past 15 years. These are predominantly red and white cattle, optimized for both milk and meat production. The average annual production is 1 350 000 litres of milk and 250 tons of beef. The agricultural farm can be described as being a strong perfomer with a very stable base, good reputation and very attractive products, both of crops and livestock. In order to keep this enviable status, frequent internal and external environmental analyses are undertaken.

The purpose of the situation analysis of the external environment is to find what is happening in the company environment now and in the future and how these factors will influence the activities and existence of the company. The paper deals with a situation analysis of the external environment of a specific company. This paper describes the external environment of the United Bakeries company using the STEPE analysis and in addition a situation analysis of the external environment was made. Next the probability estimation of the occurrence and influence of the factors is made for today, and for the near and long-term future.

The paper deals with the analysis of the typology of unemployed people in agriculture. Approximately 35-40% of people from this unemployment group have already no more chance to be reintegrated into the labour market. The analysis points to out the regional occurrence of this type of unemployment (less urbanised sub-mountain areas, stagnating and backward regions facing various processes of de-industrialisation, etc) and evaluates its wider socio-economic impacts. After 2000, the fragmentation of employment contracts in the corporative types of farms (agricultural co-operatives and companies) begins to appear in the agricultural sector. The category of seasonal agricultural workers with decreased labour and social protection begins to emerge as well. As a result of the strategy to cope with the situation, a certain self-supplying (subsistence farming) subculture was established in the Slovak rural areas to mitigate the difficult economic conditions of the rural households endangered by income deprivation, including the decreased purchasing power of rural population.

Previous experiences with breeding of lime tree (Tilia cordata Miller) in European countries are shown in this paper. The first results were obtained from the evaluation of juvenile planting of lime tree (Tilia cordata Miller) in the Czech Republic, founded in the School Forest Enterprise Kostelec nad Černými lesy. Lime tree clones (initially cultivated in vitro) were evaluated for growth parameters and also according to the growth form of the different clones. Individual clones were also checked for mortality.

The study analyses the growth of beech seedling fine roots and their development in the first five years. The research plots were established in 1997-2003 in community forests of Starý Plzenec (Western Bohemia, Czech Republic). The data are based on annual reviews of beech samplings extracted from surveyed plots. The whole root volume as well as the fine root volume is significantly (on a 95% significance level) growing during the first years after outplanting and there is not any disruption of growth immediately after outplanting.

The degree of the beech bark necrotic disease was evaluated in three selected localities in southern Poland, in different orographic complexes, Beskid Sądecki, Beskid Niski, and Bieszczady. The degree is expressed by means of the stem necrotising index (I_SN ), crowns necrotising index (I_CN ), and whole tree necrotising index (I_NWT ). The values of the indices laid in the following limits: I_SN 0.73-0.84%, I_CN 0.12%-0.51%, and I_NWT 0.84%-0.90%. The current status of the beech necrotic disease in Southern Poland can be considered as favourable. The values of I_SN recorded in Southern Poland were lower by 30% than the average I_SN for whole Slovakia. We also recorded the frequency of selected insect pests (Bucculatrix ulmella Zeller, Cryptococcus fagi Bärensp., and Ectoedemia liebwerdella Zimm.), which act in the beech stands as vectors of the necrotic disease. With C. fagi, we recorded a high frequency reaching even 100%. In contrast, we did not at all observe the occurrence of B. ulmella and E. liebwerdella, which are common in the beech stands in Slovakia, as well as in Hungary, Romania, and Bulgaria. In the localities studied, we recorded the occurrence of two species of parasitic fungi of the genus Nectria (Fr.) Fr. causing the beech bark necrotic disease, viz. Nectria cosmariospora Ces et De Not. and N. galligena Bres. ex Strasser.